LOCUS       NZ_LT985294             3002 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 650 plasmid RCS64_p, complete sequence.
ACCESSION   NZ_LT985294
VERSION     NZ_LT985294.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588845
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289765.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:21:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 105
            CDSs (total)                      :: 105
            Genes (coding)                    :: 101
            CDSs (with protein)               :: 101
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985294.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="650"
                     /db_xref="taxon:562"
                     /plasmid="RCS64_p"
     gene            join(2714..3002,1..20)
                     /locus_tag="GO897_RS00005"
                     /old_locus_tag="RCS64_PCDS2714D"
     CDS             join(2714..3002,1..20)
                     /locus_tag="GO897_RS00005"
                     /old_locus_tag="RCS64_PCDS2714D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134632.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_072842281.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNNWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSIDTLLEMVFKMGYKLDMDFNPLKTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(37..369)
                     /locus_tag="GO897_RS00515"
                     /old_locus_tag="RCS64_PCDS40R"
     CDS             complement(37..369)
                     /locus_tag="GO897_RS00515"
                     /old_locus_tag="RCS64_PCDS40R"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013857758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072842283.1"
                     /translation="MSNDDTEYTFKPVNAQALKALRSLPEWVQVAFVHALDSVFYGVDP
                     GMPIVHLTSIGPGVVELKINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATAT
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(955..1278)
                     /gene="mobA"
                     /locus_tag="GO897_RS00015"
                     /old_locus_tag="RCS64_P0001"
     CDS             complement(955..1278)
                     /gene="mobA"
                     /locus_tag="GO897_RS00015"
                     /old_locus_tag="RCS64_P0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1563..1757
                     /locus_tag="GO897_RS00020"
                     /old_locus_tag="RCS64_P0002"
     CDS             1563..1757
                     /locus_tag="GO897_RS00020"
                     /old_locus_tag="RCS64_P0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1886..2041)
                     /locus_tag="GO897_RS00520"
                     /old_locus_tag="RCS64_P0003"
     CDS             complement(1886..2041)
                     /locus_tag="GO897_RS00520"
                     /old_locus_tag="RCS64_P0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_197032755.1"
                     /translation="MSVGFCDARQGGGAYGKTPAALASPGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            2228..2374
                     /locus_tag="GO897_RS00550"
     CDS             2228..2374
                     /locus_tag="GO897_RS00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077251022.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKAPLAAAIGKRVYKILLT
                     QSS"
                     /name="replication initiation protein"
     Typing          complement(1288..1525)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.75"
                     /coverage="99.0"
                     /evalue="1e-116"
     Typing          1793..2297
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.907"
                     /coverage="95.0"
                     /evalue="1.06e-159"
     Typing          complement(959..1278)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.96e-62"
ORIGIN
        1 taaaaaaggc gatggtgtaa gcatcgcctt tatggattac aacaacttgt agcgtgatgt
       61 tgcggttgcc atattcttct tatcgggtcc ttgacacgtt ttcacgaacg catgcagcaa
      121 gtccacttga cctggagctt tgctgcgata aacgcaacga taagcagggc gaccattgat
      181 tttcaactca acaacaccgg gtccgatgga cgtcaagtgc acgatgggca tccccggatc
      241 cactccgtag aaaactgaat ccaatgcgtg cacgaatgca acctgaaccc actcagggag
      301 tgatctcaat gcctttaaag cctgggcatt aacaggtttg aacgtatatt cagtgtcgtc
      361 gtttgacata gcaatcctct cttaggttat tgatgtttaa caaattaact accgtattga
      421 tatttcgcat aaatactccg attaattgat ctgtgagcat aaaaaaacca caccgcccta
      481 ttttttaggt cagtgcgcgt atggtcatta gctcgtagaa ccaataatta ttggcaatct
      541 cgccaacact gacacagtat acaaatactt atatgcaatc aagttttatc aaaggtatac
      601 aaaactaacc gctcaaaaaa aagcggttta ccttaagtca tgacactaaa gagggttatt
      661 taggcaaaca gaactgtagc cagagaagcc cgattgctag ataaaaacca atgaagaaac
      721 ctcgccaatg atagatttac cataatccct taattgtacg caccgcagaa acgcgctgaa
      781 cgggattctg ccgcagacac cgcaaaaaga gatgtgttac caagcgaaaa ccggaaaaat
      841 gcgtcaggat cgttttcagc tcgctgcata gctatgcatg aaagcgaatg gtgatcactt
      901 tgggagctta cggtgttcat accgtctgtt ttcgacagtt ttctctccgg gaagctaatc
      961 tgccataagc ctggataaca gggcacggtg atactccgta atagcgatca gcacctccgc
     1021 atactccctg tccccgacac gcttgccgtc gataaagagt tttttctgta acgcccccag
     1081 tctcataact tctttcagta cccgatcatc agtcagtgag ttaactttct taccgagagc
     1141 tgccgcccgt aaaaaaccgg aaacggtctt gccgcattca gcagcccttt ttctgatttc
     1201 ctggtcttct tctgccgtca gacgaacagg gcgactgata gcccgcctgc gcgtattact
     1261 tccacttcgt tttgtcataa aacatggctc cgtatctgac atggggtgtc ggggcgaagc
     1321 cctgaccagg gtaattgtaa tagcgtgcat gtatgcgcgg tataacaatt acacatcctg
     1381 tcctgtcagc aagttcgaat taccgacaaa ggcacggcag ttttttggca gtgacagtat
     1441 tgtcaggcag aaatgcccgt caaagagtca gacggcagaa taccggcggt aaaacagtcg
     1501 aaaagtggta cgcgatataa tcacacaacg cagatgatag acagaacgga gaaaacgaaa
     1561 agatgaacaa gcagcagcaa gccgtactca acatggccgg attcataaaa agccagagcc
     1621 tgacgctgct cgaaaaactg gacgcactcg atgctgacga acaggccgcc atgtgtgaga
     1681 agctgcacga actcgcggaa gaacttcaga acagcataca gacacgcttt gaagcggaaa
     1741 atcgcaccgg gatataacgc atccggacag gatgcagaac ggcatagagg agcgtaagga
     1801 gaaaatgtca cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg
     1861 ttcgactgcg gcgagcggtg tcagctcact caaaagcggt aatacggtta tccacagaat
     1921 caggggataa cgccggaaag aacatgtgag caaacagcaa agcaccggga gaagccagcg
     1981 ccgcaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa ccgacgctca
     2041 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2101 tccctcgtgc gctctcctgt tcctgccttt cggtttacca atgtcattcc gctgttatgg
     2161 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     2221 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg gtaactatca
     2281 acttgagtcc aacccggaaa gacacgacaa agcgccactg gcagcagcca ttgggaagag
     2341 ggtgtacaag atattactga cgcagagttc ttgaagtcag agccaaacta cagctaaact
     2401 aaaaagacag aatttggttt cagcggtctt gtacaccctg ttaccatggt tcagaagttc
     2461 cccaactcac tgaaccctca aaaaaaccgc ctccccaggc ggtttttttg tttcttgaac
     2521 aagactactc tttgcttaag agtcacgtca ataccatcat tgttacttgc aagatcaaat
     2581 gcaattcaca tctttttctc ttgaaagaga tatcattact tgtataatgc cccgcccata
     2641 tatatatata taaggagttc cttgtcggaa cttttatcat cgaggtacag gtaatccaac
     2701 atggagctgt tctatgaaaa attcatttga gctgataagt accgacccgg tgaaattcaa
     2761 cacatggtcg ataaaatcaa aactcataat aataatcaga aagttaatga tcgataacaa
     2821 ttggtctcaa tctgaagcag ctgaaaagtt aggtatcagt cagccccgag taagtaacat
     2881 gctgaacggg aagctagaca aattctccat cgacacattg ttagaaatgg tgttcaagat
     2941 gggttacaag ctagatatgg atttcaaccc attaaaaaca gaatcaccac tcacaatggt
     3001 tg
//
