LOCUS       NZ_LT985285             4054 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 13948 plasmid RCS76_pIII, complete sequence.
ACCESSION   NZ_LT985285
VERSION     NZ_LT985285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588797
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289845.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:52:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 139
            CDSs (total)                      :: 139
            Genes (coding)                    :: 118
            CDSs (with protein)               :: 118
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 9 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="13948"
                     /db_xref="taxon:562"
                     /plasmid="RCS76_pIII"
     gene            complement(464..718)
                     /gene="mobC"
                     /locus_tag="GWA62_RS00060"
                     /old_locus_tag="RCS76_PIII0001"
     CDS             complement(464..718)
                     /gene="mobC"
                     /locus_tag="GWA62_RS00060"
                     /old_locus_tag="RCS76_PIII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            897..3044
                     /gene="mobQ"
                     /locus_tag="GWA62_RS00065"
                     /old_locus_tag="RCS76_PIII0002"
     CDS             897..3044
                     /gene="mobQ"
                     /locus_tag="GWA62_RS00065"
                     /old_locus_tag="RCS76_PIII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_015059934.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKVAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQKWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFERVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATVRRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3290..3990)
                     /locus_tag="GWA62_RS00070"
                     /old_locus_tag="RCS76_PIII0003"
                     /pseudo=""
     CDS             complement(3290..3990)
                     /locus_tag="GWA62_RS00070"
                     /old_locus_tag="RCS76_PIII0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(720..896)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          897..1255
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3292..3990)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.287"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          811..3041
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.326"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attttaatat ccctttttct gtgaatatta ccttaaatca tattaccttt accttaaatc
       61 atattaccta aacaccttaa atcatattac ctttacctta aatcatatta cctatagcgt
      121 tgtaacattt tgttttctat aaggaatttt ggcactaaaa caagtattaa aaacaagatc
      181 ttaaaaacaa gatctaaaaa cttaacgacg agcctgtgga taacttggag tgctgaaaac
      241 aaaaaaccaa taaaaacaat gcgttgcaaa aaagcagaat tgcaagttcc gaatacaccc
      301 cctccaaccc tgtggaaatc cagtattcat gcgggttcag cgcatatcac ctcccgaaaa
      361 accgtgcgta tgtgctcaat caggcgacgg attcacttcc gcttcctcgc ccactcgctc
      421 gctaacgctc ggtcgctggg actgcggcga gcggacgata caatcactgt tctggcagcg
      481 cttccagctc gttaatcagc ggcagtatgc gcgtcacatc agcctgtcgt gtcacctctg
      541 ccttcaggaa ctggagtaac caggcacgca actgcacctc ctccggcttt ctgacctttg
      601 ccagcaatgc tgcacccaca atgaccttct gcgctgtgtc cagactcctg ctcttcgcct
      661 tcaggcgctg taatctggcc tcagcatcag caatcttctg ttcgagtgtt ctgctcatga
      721 tgtaactccg tgcgcgatga aaaatcgcat tttagcgcgt cactggtagt ttaaaaactg
      781 agctggcata atgcacggca aatcacgaag tgcgcactta tacaatctcc actccgtttc
      841 gattgtgtgc gccctgcggg gctgaaagaa aaccacaaaa aggcattacg gcagaaatgg
      901 cgatttatca tctcagcatg aaaatcatct ccagaaacag cggctacagc gccgttgctt
      961 ctgctgccta tcgttccggt tcactgatgc ttgatgagcg taccggactg actcacgact
     1021 acacccgcaa aagcggtgtg gctgaagctg tgattctgac tcccgcgaca gcccctgcct
     1081 ggtgtacgaa ccgtgccgaa ctctggaatg ccgttgagaa ggccgaacgc cggaagaact
     1141 cacaacttgc gcgggaaatt gaactcgcca tcccccacga actgccgcag gatgccgcac
     1201 gggaaaccgt tctcgccttt gtcagagaaa actttgtcag tcagggcatg attgctgatg
     1261 tggcgttcca tcacatggat aaaacaaatc cccatgcgca catcatgctg accacgagag
     1321 ctgtcggacc tgctggattc ggtggaaagg tcagggactg gaacgaccgg acacatgccg
     1381 agacgtggcg ggcatcatgg gctgaccatg caaacagggc actggcgaac gccggttatc
     1441 aggaagagat agaccatcgt tcatacgagc gacaggggct tgagaaaacg cctggtattc
     1501 acctcggaaa gtctgcctgt gcgatggaaa cacgcggtat cgaaacagaa cgcggcgaac
     1561 agaatcgtct gattaacagg cttaacctgg aaatacagat ttcccgcacc gaactcagga
     1621 acggagggct gacagagcct gcccgccgtg tggctgacct tctgaatatc gggcttcctg
     1681 acaacgcaac ttcgtacaca ctacgtgaca tcatcgaagc gttgccgaag gacagtaact
     1741 cggcatggaa actcaccagt aatgccatgt cgatgatggc agatatgcag gcgacgcgca
     1801 gacgctggga agagctgaac accgaacgaa aggtagcgat gagccatgct gccagcctga
     1861 aaaaatcatc gccgttttcg tccggttttt cccgtattcc cctgatgcaa tgggcggcac
     1921 cggaataccg tagtgagcag ggaaaaatcc agtctctgag ccagcaaatg gaaaaattgc
     1981 gacagcatta ccggaaggtg gagaaacagg acattcccgc gagtcagatg gcatttgaaa
     2041 aacagtggca gaagtggggg gaatccggcc ttgcagctct cagagcccag ttgcttaaac
     2101 gtgaagagga attgcgccgg aaggaacagg aagatactga acgtagtcgc gcggagcagc
     2161 tcaggaaaaa tgacaatgcc gttatggatt caggggtact gagcgcagtc gttacggggt
     2221 atggcgaggc ccccatgcct ggtaacgggg aaatgacctg ttaccttctg cttcacaacc
     2281 ggaacggaga atatacgctc tgggggaacg aactggagaa ataccgcgcc agaatctttg
     2341 agcgtgttga tttaatgcgt gacaggagtg gttatatctg tgaccgaagc gaaatcggcc
     2401 aacgtccgcc actgcaacgt gtttattcct ctgcgacctt tgagcaactg ctgacgcagg
     2461 tttgccagac atggccgcaa catacccgca atctccgcca gccgaagaca tggccggagt
     2521 ctttctgtct tggagaagac cgccagccag ccatgccatc actggcagca aggaaggtgg
     2581 attttacgca gggacgtctg ccaccgacgc tgatgccggt aatgagttct gtagaccggg
     2641 aaacccgaca acttcagctt ttgctggtca tgggcgtgga tgactctctg ggcggtgttg
     2701 tccggcttaa tggtacgctg tatcccgcgt ttgccgttcc ctcagccgat aacagccagc
     2761 tggtcatcag tgcactgacc gataaggggt tacggtatgc agggtatggt gttgccgtaa
     2821 atcacgatgc agacagccac atatcacctg caccagaact gatgcagttt catctgaaaa
     2881 cccgtgaggc accgcttttt gctgccgtca acacaccaga aaaacagccg gatcatctgt
     2941 tccgctcgct gggttttaat cgtacctggg acgaatggcg gcgcgaggaa gatgcacgaa
     3001 cacacgccac cgtgaggaga catgatcgcg gttggtcaca gtgaatgtga acaatatttt
     3061 gttttgtgta acatcgtgtt atgttcgccg atgttcatcg acagggatga atgcaagaag
     3121 cacactcaca taacgcaaac atagttttgt ttttccgccc ccaaaattgg gggttctttt
     3181 ttggcctctc aaatcaattc tgacgcaatt tattgccaaa gcagtaccat cgcatacctg
     3241 acaccaaaaa aacgcaccag agaacgtttt agaggcctta aaatcgattc tagatcgtca
     3301 tcttgatctg tttgtcttcc ttgaagcgaa aatgcagggc tacaaccgtg cgcccctttt
     3361 tgatcgtctc aaccgttaca accagatcgc ttttttgttc agttcgtcaa tgcagggtct
     3421 taataattgc tggttaagtg atttatatgt ttgatattta tgctctatat caagcattga
     3481 gcgaaaatca tcgagagcga taatacgttc gccagtactg cggaactgct ggaggagttc
     3541 gtatatcctt atactgtaac ttcttgataa tctagatata ttcttgatta caactctagt
     3601 aaattgccct tgtagttgtg tcaaataagg cattactgcg tcagaaaatg ttatttgtgc
     3661 tctggcctcg cctttagcgt actgtgcccg atactggatc cagcggaact cctcacgttt
     3721 ctcgtcatcc ttgagaatga ttgacctgtc ccacaaacga tcaattgctt cctgtaactg
     3781 aacctcggca tttttccgcc ccatgtcagg gaatgaatcg aaatattccg cagctgtaat
     3841 ggtcattgtc ttttgcaatt ttggagattc tgcatcagcc ccagacttca atcgaccgat
     3901 gcaaagtaac aaaaatcgct gttcctgtag ggtcaattta taactggctt caaccagcgc
     3961 attagattta taggcaatta gatccgacat agagcatccc tccccaaaag gtaatgaata
     4021 aaagataaac atacccccag cgaaaggcaa tgcc
//
