LOCUS       NZ_LT985255             4074 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 580 plasmid RCS49_pII, complete sequence.
ACCESSION   NZ_LT985255
VERSION     NZ_LT985255.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588836
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289585.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:29:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 137
            CDSs (total)                      :: 137
            Genes (coding)                    :: 105
            CDSs (with protein)               :: 105
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 22 of 32
            Pseudo Genes (incomplete)         :: 12 of 32
            Pseudo Genes (internal stop)      :: 0 of 32
            Pseudo Genes (multiple problems)  :: 2 of 32
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985255.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="580"
                     /db_xref="taxon:562"
                     /plasmid="RCS49_pII"
     gene            64..318
                     /gene="mobC"
                     /locus_tag="GWB06_RS00090"
                     /old_locus_tag="RCS49_PII0001"
     CDS             64..318
                     /gene="mobC"
                     /locus_tag="GWB06_RS00090"
                     /old_locus_tag="RCS49_PII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            850..1542
                     /locus_tag="GWB06_RS00095"
                     /old_locus_tag="RCS49_PII0002"
     CDS             850..1542
                     /locus_tag="GWB06_RS00095"
                     /old_locus_tag="RCS49_PII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1800..3959)
                     /gene="mobQ"
                     /locus_tag="GWB06_RS00100"
                     /old_locus_tag="RCS49_PII0003"
     CDS             complement(1800..3959)
                     /gene="mobQ"
                     /locus_tag="GWB06_RS00100"
                     /old_locus_tag="RCS49_PII0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(3602..3960)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3961..4074
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.899999999999999e-53"
     Typing          851..1542
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.821"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1804..4046)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagttttta aactaccagt gacgcgctaa aatgcgattt ttcatcgcgc acggagttac
       61 atcatgagca gaacgctcga acagaagatt gctgatgctg aggcgagatt acagcgcctg
      121 aaggcgaaga gccggagcct ggacacagcg cagaaggttg ttgtgggtgc agcattgctg
      181 gcaaaggtca gaaagccgga ggaggtgcag ttgcgtgcct ggttactcca gttcctgaag
      241 gcagaggtga cacgacaggc tgatgtgacg cgcatactgc cgctgattaa cgagctggag
      301 gcgctgccag gacagtgatt gtatcgtccg ctcgccgcag ccccagcgac cgagcgttag
      361 cgagcgagtg ggcgaggaag cggaagtgaa tccgtcgcca ggttgagcac atacgcacgg
      421 tttttcggca ggtgatatgc gctgaacccg catgaatact ggatttccac agggttggaa
      481 ggggagtatt cggaacttgc aattctgctt tcttgcaaca cattgttttt attggttttt
      541 tgttttcatc cccccaagtt atccacaggc tcgccgttaa gtttttagat cttgttttta
      601 agatcttgtt tttaatactt gttttagggg tgaaattcat taataaaatc aatatgttat
      661 agtggtatat gtggaggaat ttaaggtaaa ggtggaggaa tttaaggtgt ttaggtggag
      721 gaatttaagg taaaggtgga ggaatttaag gtgttattca cagaaatgtg aggatataaa
      781 aaaaaacatt ccacatttct tatttgtggg gcacattaaa gcatcaccac atttgaacgg
      841 gatctttgta tgtcggactt gattgcctac aagtcgaatg ctttggttga ggccagctac
      901 aagttgaccc tacaggaaca acgttttctg ttgctgtgta tcagtcgatt gaaatctggt
      961 gctgatgctg aattgcaaaa gacaatgacc attaccgctg cggaatattt tgattcattc
     1021 cctgacatgg ggcggaaaaa tgccgaggtt cagttacagg aagcgatcga tcgtttatgg
     1081 gacaggtcga tcattctcaa gaatgacgag aagcgtgagg agttccgctg gatccagtat
     1141 cgggcacagt acgctaaagg tgaggccaag gctcgaataa ccttttctga cgcggtaatg
     1201 ccttatctga cgcaactgaa ggggcaattc acgcgcgttg ttataaaaaa catatcaggt
     1261 ttatcaagta gttacagtat aagaatatac gagttcctcc agcaattccg cagtacggga
     1321 gagagaatta ttgctcttaa tgactttcgt tcaatgttag ggattgaaaa taaatacaaa
     1381 caattcagag acttaaataa aatactaata aaaccctgtg ttgacgaact gaacaaaaaa
     1441 agcgacctgg ctgtaacggt tgagacgatc aaaaaggggc gcacggttgt agccctgcat
     1501 tttcgcttca aggaagataa acagatcaag atgacgatct aaaatcgatt ttaaggcacc
     1561 taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa
     1621 aaattgcgtc aaaaacgctc tgatgcagcg aaaaaagaac ccccaatttt tgggggcgga
     1681 aaaacaaaac tatgcttgca ttatgtgagt gtgcttcttg cattcatccc tgtcgatgaa
     1741 catcgaagaa catcgaagaa cataacacga tgttaaacaa aacaaaatat tgttcacatt
     1801 cactgtgacc aaccgcgatc atgtctcctc tcggtggtgt gtgttcttgc atcttcctcg
     1861 cgccgccatt cgtcccaggt acgattgaaa cccagcgagc ggaacagatg atccggctgt
     1921 ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac gggttttcag atgaaactcc
     1981 ataagttccg gcgcaggtga tatgtggctg tctgcatcgt gatttacggc aacaccatac
     2041 cctgcatacc gtaacccctt atcggtcagt gcactgatga ccagctggct gttatcggct
     2101 gagggaacgg caaacgcggg atacagcgta ccattaagcc ggacaacacc gcccagagag
     2161 tcatccacgc ccatgaccag caaaagctga agctggcggg tttcccggtc gacagaactc
     2221 attaccggca tcagcgtcgg tggcagacgt ccctgcgtaa aatccacctt ccttgctgcc
     2281 agtgatggca tggctggctg gcggtcttct ccaagacaga aagactccgg ccatgtcttc
     2341 ggctggcgga gattgcgggt atattgcggc catgtctggc aaacctgcgt cagaagttgc
     2401 tcaaaggtcg cagaggaata aacacgttgc agtggtggat gttgaccgat ttcgcttcgg
     2461 tcacagatat aaccactcct gtcacgcatt aaatcaacac gctcaaagat tctggtgcgg
     2521 tatttctcca gctcgttccc ccagagcgtg tattctccgt tccggttgtg aagcagaagg
     2581 taacaggtca tttccccgtt accaggcatg ggggcctcgc cataccccgt aacgactgcg
     2641 ctcagtaccc ctgaatccat aacggcattg tcatttttcc tgagctgttc cgctcgtcgc
     2701 tccgcgcgac tacgttctgt atcctcctgc tccttccggc gcaattcctc ttcacgttta
     2761 agcaactgga ctctgagagc tgcaaggccg gatgcccccc actgctgcca ctgtttttca
     2821 aatgccgtct gactcgcggg aatgtcctgt ttctccacct tccggtaatg ctgtcgcaat
     2881 ttttccattt gctggctcag cgactggatt tttccctgtt cgctacggta ttccggtgcc
     2941 gcccattgca tcaggggaat acgggaaaaa ccggacgaaa acggcgatga ttttttcagg
     3001 ctggcagcat ggctcatcgc ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc
     3061 gtcgcctgca tatctgccat catcgacatg gcattactgg tgagtttcca tgccgcatta
     3121 ctgtccttcg gcaacgcttc gatgatgtca cgtagtgtgt acgaagttgc gttgtcagga
     3181 agcacgatat tcagaaggtc agccacacgg cgggcaggct ctgtcagccc gctgttcctg
     3241 agttcagtgc gggaaatctg tatttccagg ttaagcctgt taatcagacg attctgttcg
     3301 ccgcgttctg tttcgatacc gcgtgtttcc atcgcacagg cagactttcc gaggtgaata
     3361 ccaggcgttt tctcaagccc ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa
     3421 ccggcgttcg ccagtgctct gtttgcatgg tcagcccatg atgcacgcca cgtctcggca
     3481 tgtgtccggt cgttccagtc cctgaccttt ccgccgaacc cagcaggtcc gacagccctc
     3541 gtggtcagca tgatgtgcgc atggggattt gttttatcca tgtgatggaa cgccacatca
     3601 gcaatcatgc cctgactgac aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg
     3661 gcatcctgcg gcagttcacg ggggatagcg agttcaattt cccgcgcaag ttgtgagttc
     3721 ttccggcgtt cggccttctc aacggcattc cagagttcgg cacggttcgt acaccaggca
     3781 ggggctgtcg cgggggtcag aatcacagct tcagccacac cgcttttgcg ggtgtagtcg
     3841 tgagtcagtc cggtacgctc atcaagcatt agtgaaccgg aacgataggc agcggaagct
     3901 acggcgctgt agccgctgct tctggagata attttcatgc tgagatgata aatcgccatt
     3961 tctgccgtaa tgcctttttg tagttttctt tcagccccgc agggcgcaca caatcgaaac
     4021 ggagtggaga ttgtataagt gcgcacttcg tgatttgccg tgcattatgc cagc
//
