LOCUS       NZ_LT985247            19813 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 720 plasmid RCS48_pII, complete sequence.
ACCESSION   NZ_LT985247
VERSION     NZ_LT985247.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588835
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289525.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:30:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 118
            CDSs (total)                      :: 118
            Genes (coding)                    :: 106
            CDSs (with protein)               :: 106
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985247.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="720"
                     /db_xref="taxon:562"
                     /plasmid="RCS48_pII"
     gene            <1..117
                     /locus_tag="GWB39_RS00035"
                     /pseudo=""
     CDS             <1..117
                     /locus_tag="GWB39_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     gene            complement(879..1097)
                     /locus_tag="GWB39_RS00040"
                     /old_locus_tag="RCS48_PII0001"
     CDS             complement(879..1097)
                     /locus_tag="GWB39_RS00040"
                     /old_locus_tag="RCS48_PII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041626.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFEAESGTRQDAERYTCA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1369..1692
                     /gene="mobA"
                     /locus_tag="GWB39_RS00045"
                     /old_locus_tag="RCS48_PII0002"
     CDS             1369..1692
                     /gene="mobA"
                     /locus_tag="GWB39_RS00045"
                     /old_locus_tag="RCS48_PII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(1917..2296)
                     /gene="lsoB"
                     /locus_tag="GWB39_RS00050"
                     /pseudo=""
     CDS             complement(1917..2296)
                     /gene="lsoB"
                     /locus_tag="GWB39_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /name="lsoB"
     gene            complement(2293..3332)
                     /gene="lsoA"
                     /locus_tag="GWB39_RS00055"
                     /pseudo=""
     CDS             complement(2293..3332)
                     /gene="lsoA"
                     /locus_tag="GWB39_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /name="lsoA"
     gene            complement(3596..3709)
                     /locus_tag="GWB39_RS00615"
     CDS             complement(3596..3709)
                     /locus_tag="GWB39_RS00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(4289..7294)
                     /locus_tag="GWB39_RS00060"
                     /old_locus_tag="RCS48_PII0006"
     CDS             complement(4289..7294)
                     /locus_tag="GWB39_RS00060"
                     /old_locus_tag="RCS48_PII0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            7458..8015
                     /locus_tag="GWB39_RS00065"
                     /old_locus_tag="RCS48_PII0007"
     CDS             7458..8015
                     /locus_tag="GWB39_RS00065"
                     /old_locus_tag="RCS48_PII0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            8198..9058
                     /locus_tag="GWB39_RS00070"
                     /old_locus_tag="RCS48_PII0008"
     CDS             8198..9058
                     /locus_tag="GWB39_RS00070"
                     /old_locus_tag="RCS48_PII0008"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            <9219..9716
                     /locus_tag="GWB39_RS00075"
                     /old_locus_tag="RCS48_PII0009"
                     /pseudo=""
     CDS             <9219..9716
                     /locus_tag="GWB39_RS00075"
                     /old_locus_tag="RCS48_PII0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /name="mercuric reductase"
     gene            9734..10096
                     /gene="merD"
                     /locus_tag="GWB39_RS00080"
                     /old_locus_tag="RCS48_PII0010"
     CDS             9734..10096
                     /gene="merD"
                     /locus_tag="GWB39_RS00080"
                     /old_locus_tag="RCS48_PII0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0006355 -
                     regulation of DNA-templated transcription [Evidence IEA];
                     GO:0046689 - response to mercury ion [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="WP_001277456.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDDA
                     ALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHLDA
                     QLASMPAERAHEEALP"
                     /name="merD"
     gene            10093..10329
                     /gene="merE"
                     /locus_tag="GWB39_RS00085"
                     /old_locus_tag="RCS48_PIICDS9832D"
     CDS             10093..10329
                     /gene="merE"
                     /locus_tag="GWB39_RS00085"
                     /old_locus_tag="RCS48_PIICDS9832D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="WP_000993386.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTAG
                     AFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
                     /name="merE"
     gene            10365..11033
                     /locus_tag="GWB39_RS00090"
                     /old_locus_tag="RCS48_PII0011"
     CDS             10365..11033
                     /locus_tag="GWB39_RS00090"
                     /old_locus_tag="RCS48_PII0011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="WP_001300294.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPPG
                     QFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEVKR
                     VLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHLKCC
                     PITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCCAASS
                     "
                     /name="EAL domain-containing protein"
     gene            11108..>12358
                     /locus_tag="GWB39_RS00095"
                     /old_locus_tag="RCS48_PII0012"
                     /pseudo=""
     CDS             11108..>12358
                     /locus_tag="GWB39_RS00095"
                     /old_locus_tag="RCS48_PII0012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            12423..13127
                     /locus_tag="GWB39_RS00100"
                     /old_locus_tag="RCS48_PII0013"
     CDS             12423..13127
                     /locus_tag="GWB39_RS00100"
                     /old_locus_tag="RCS48_PII0013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067834.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YISSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <13177..13353
                     /locus_tag="GWB39_RS00105"
                     /pseudo=""
     CDS             <13177..13353
                     /locus_tag="GWB39_RS00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /name="STAS domain-containing protein"
     gene            13388..>13489
                     /locus_tag="GWB39_RS00620"
                     /pseudo=""
     CDS             13388..>13489
                     /locus_tag="GWB39_RS00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787802.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /name="universal stress protein"
     gene            13545..14242
                     /locus_tag="GWB39_RS00110"
                     /old_locus_tag="RCS48_PII0015"
     CDS             join(13545..13794,13794..14242)
                     /locus_tag="GWB39_RS00110"
                     /old_locus_tag="RCS48_PII0015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_240533048.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKQHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            14244..15764
                     /locus_tag="GWB39_RS00115"
                     /old_locus_tag="RCS48_PII0017"
     CDS             14244..15764
                     /locus_tag="GWB39_RS00115"
                     /old_locus_tag="RCS48_PII0017"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_001301397.1"
                     /translation="MESLPSPQSHITYVNPDFIKISGFTEEELLGQPHNIVRHPDMPPA
                     AFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAKNGSIVEYQSVRTKPEPEQV
                     LAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSAMAAGMLTDTSISSLLLATL
                     MSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGRTDEFGQIEFALRMMQAETG
                     AIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQIATAVNQMVASIQEVASNA
                     QHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQVIHELEGQSNEISKVLDVI
                     RGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAARTQQSTTDIQSMISALQER
                     AQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEITDMNAQIATAVEQQGAVSED
                     INRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELAKQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            16092..16853
                     /locus_tag="GWB39_RS00120"
                     /old_locus_tag="RCS48_PII0018"
     CDS             16092..16853
                     /locus_tag="GWB39_RS00120"
                     /old_locus_tag="RCS48_PII0018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226821.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="WP_002210513.1"
                     /translation="MIPIERHQRILALVEQRGAVSINELTEILGVSHMTIRRDVSKLEE
                     QGLLVSVSGGVRAVSRLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTT
                     LALARAILDRNDLQVVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRH
                     LAIDTAFISASGWDSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPL
                     TRFTTIITDRHLSDAAASHIARHACEVLRAG"
                     /name="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            complement(16874..17734)
                     /locus_tag="GWB39_RS00125"
                     /old_locus_tag="RCS48_PII0019"
     CDS             complement(16874..17734)
                     /locus_tag="GWB39_RS00125"
                     /old_locus_tag="RCS48_PII0019"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase SHV-12"
                     /protein_id="WP_002904004.1"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIEM
                     DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPV
                     SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWET
                     ELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLP
                     AGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALI
                     EHWQR"
                     /name="extended-spectrum class A beta-lactamase SHV-12"
     gene            17871..18575
                     /locus_tag="GWB39_RS00130"
                     /old_locus_tag="RCS48_PII0020"
     CDS             17871..18575
                     /locus_tag="GWB39_RS00130"
                     /old_locus_tag="RCS48_PII0020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            18805..19665
                     /locus_tag="GWB39_RS00135"
                     /old_locus_tag="RCS48_PII0021"
     CDS             18805..19665
                     /locus_tag="GWB39_RS00135"
                     /old_locus_tag="RCS48_PII0021"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     ARG             8199..9055
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             18806..19662
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(16878..17734)
                     /name="blaSHV-12"
                     /gene="blaSHV-12"
                     /gene_name="extended-spectrum class A beta-lactamase
                     SHV-12"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2..117
                     /name="TEM-238"
                     /gene="TEM-238"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="100.0"
                     /coverage="13.29"
     Typing          1135..1360
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.75"
                     /coverage="99.0"
                     /evalue="2.28e-73"
     Typing          complement(361..885)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.075"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(3638..4168)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.796"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1370..1689
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.9500000000000006e-64"
ORIGIN
        1 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
       61 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
      121 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
      181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
      241 tcgttccact gagcgtcaga cccctttctt tcagtttagc cttaaccggc gcataacatc
      301 aagactaact cctctaaatc agttaccaat ggctgctgcc agtggcgata agtcgtgtct
      361 ttccgggttg gactcaaggc gacagttacc ggataaggcg cagcggtcgg cgtgaacggg
      421 tggttcggtg cacacagccc agcttggagc gacgacctac accgggccga gataccaaca
      481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      541 aaacggcagg gtcggaacag gagagcgcac gagggagctt ccggggggaa acgcctggta
      601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      661 gtcagggggg cggagcctat ggaaaaacgc cataggcgcg gccttttcct ggttctcgtt
      721 ttttgctcac atgctccttt tggctttatc ccctgattct gtggatcacc gcattaccgt
      781 ttttgagtga gctgataccg ctcgccgcag ccgaacggcc gagcgtagcg agtcagtgag
      841 cgaggaagcg gaagagagcc ttatgtgtca ttttctcctt acgcacaggt ataccgttct
      901 gcatcctgtc tggtgccact ttccgcttca aagcgtgtct gtatgctgtt ctggagttct
      961 tctgcgagtt cgtgcagttt ctcacacatg gcggcctgtg catcagcatc gagtgagtcc
     1021 agtttttcga gcagcgtcag gctctggctt tttatgaatt ttgccatgtt gagtgcggtt
     1081 tgctgctgct tgttcatctt tctgttttct ccgttctgtc tgtcatctgc gtcgtgtgat
     1141 tatatcgcgc accacttttc gaccgtctta ctgcaggtat tctgccgacg gacatttcag
     1201 tcagacaaca ctgtcactgc caaaaaacag cagtgctttg ttggtaattc gaacttgcag
     1261 acaggacagg atgtgcaatt gttataccgc gcatacatgc acgctattac aattaccctg
     1321 gtcagggctt cgccccgaca ccccatgtca gatacggagc catgttttat gacaaaacga
     1381 agtggaagta atacgcgcag gcgggctatc agtcgccctg ttcgtctgac ggcagaagaa
     1441 gaccaggaaa tcagaaaaag ggctgctgaa tgcggcaaga ccgtttctgg ttttttacgg
     1501 gcggcagctc tcggtaagaa agttaattca ctgactgatg accgggtgct gaaagaagtc
     1561 atgcgactgg gggcgttgca gaaaaaactc tttatcgacg gtaagcgtgt cggggacaga
     1621 gagtatgcgg aggtgctgat cgctattacg gagtatcacc gtgccctgtt atccaggctt
     1681 atggcagatt aacttcccgg agagaaactg tcgaaaacag acggtatgaa cgccgtaagc
     1741 ccccaaaccg atcgccattc actttcatgc atagctatgc agtgagctga aagcgatcct
     1801 gacgcatttt tccggtttac cccggggaaa acatctcttt ttgcggtgtc tgcgtcagaa
     1861 tcgcgttcag cggtgcgtac aatgagacgt tatggtaaat gtcttctggc ttgatattat
     1921 attggaatgc cttttttcaa agcaaatgat gtggctttgg atagaaggtt tacgttgatc
     1981 ttatcaaagt tttttttaaa gaacgaagcc gagagctcag ataaatcatt atattcatca
     2041 gttttcgtaa ctttgtttaa tgtgtaactt gaaaacttct cgccattaaa tgacgtatag
     2101 acgtaacgat ctttttttcc accgttagga attattaaat caaaaaaaca tcacccttgc
     2161 ttttcttttt cttcaagtcg gattcgattt ttgagaaaaa ttcgctcggg ctataaatat
     2221 cagtagcata gacaataaat aaagttttat ctttattttt tattgcttct atttgatatt
     2281 ttttatcttt tttcataatt tcaacctagc tacgccacca tctattaatt ggcaaacggt
     2341 atcgatgatt gcgattgatt ctaatttgtt aattgtcttc gtgtcagcta ttcctggttt
     2401 catatgaaac aaaccatgcc tgttctcatg ccagtaagtg tagcattcac acaaaacttc
     2461 cgctatttca ccatttatag tttcttggtg tatttctctg attatatatt tgggtttgtt
     2521 ttcagtgaag tattcgccaa ggttctttga tgatgatgga ttgcaaacat cattaagtat
     2581 ttgatatata aagccttcta tggctcttaa tgcagagaag cagtatgttg agtaatcttc
     2641 catttcgaca tctatttttt tcattattag cgagcatgat agctgttttt tgatatcttc
     2701 atggatttta tcgatgcttt ttggtagttt gctatgcaac tcggactcaa tagtttcttt
     2761 tttatgtcaa cattaaattc tttatttttt tgttcgacaa tctctttcat gtttagtatt
     2821 gagcacatga aatcgttaat caaactcgcg atttgaaggt attttccttg gaattgaata
     2881 gagccgcgct tgtaaatttt tgccctgacc ctgtcaccat tgctggtggt cataatatat
     2941 tggtgtttac aattaggatc gttattatta tcttctgtta ttgttatccc ctcttcagaa
     3001 agaaattcaa atagatttgc cctgtcatca tcactgaatt ttggaatggt gtattcaaag
     3061 ttctttgtgt ctgaatacaa acagttttct tttataatca aggcgatttc atcaaagtaa
     3121 gtgttatttt gcccagacgc tcttccgata gtggtatttc cgcctgatgg cattagtttt
     3181 attaagaagt ctattccttt atatgtgcca gatatgtgag tttctctttc gttttttaca
     3241 ttagaggaat agtttgtgac gccattctgc gtcagagcag actcaatctt gtcaatattg
     3301 atatttagtg ctttaaacgg gttctgtgcc attgggtcaa tccgttgttt ttttgaatat
     3361 gtacagatct tgtttttttg tcaacggaat agctgttcgt tgacttgata gaccgattga
     3421 ttcatcatct cataaataaa gaaaaaccac cgctaccaac ggtggttttc tcaaggttcg
     3481 ctgagctacc aactctttga accaaggtaa ctggcttgga ggagcgcagt caccaaaatc
     3541 tgttctttca gtttagcctt aacaggtgca taacttcaag acaaactcct ctaaatcagt
     3601 taccaatggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3661 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3721 tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca
     3781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3901 gccacctctg gcttgagcgt cgcatttttg tgatgctcgt caggggggcg gagcctatgg
     3961 aaaaacgcct gcggcgttgg cttcttccgg tgctttgctt tttgctcaca tgttctttcc
     4021 ggctttatcc cctgattctg tggataaccg tattaccgct tttgagtgag ctgacaccgc
     4081 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aagagcgcct
     4141 tatgtgacat tttctcctta cgctctgttg tgccgttcgg catcctgtcc tgagagttat
     4201 ctctctgtgc tattttctac ttcaaagcgt gtctggatgc tgttctggag ttcttggggt
     4261 ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt ttctgcctct gacgcctctt
     4321 ttaatggtct cagatgtcct ttggtcacca attctgccag cgtgaaggaa taatggccga
     4381 gcatattgat atgtccgtgg caaagcgggg agaggcgtgc gatatcttca tcattcagtg
     4441 tttcaccctg cgcccggaga tgatccaggg ctgcctgcat ataaatagtg ttccataaca
     4501 cgacggcgtt agtgaccagc cccagtgtgc ccagttgatc ttcctgaccg tcggtatatc
     4561 gttttcttat ctcacctttt tgaccgtgac agatggctct ggcaacggca tggcgacttt
     4621 ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc ttcatcatca atataattaa
     4681 gcagatacag cgttttgttg atgcgcccca cttcaatgat tgcctgagtc agtccggaag
     4741 gacgttcact tttcagcaat gaacggacca gcactgaaac ctgtactttg cccagcttca
     4801 gggagccagc ggtccggatc atttcgtccc actgaaggac tatttttcgg ggatctgatt
     4861 gccctctggc aatatcattc agcacgccat agtcggcatc atggtccatt cgccagaaaa
     4921 ccgaagcacc ggcatcagcc aggcgtggag aaaactggta tcccagcagc cagaaaaggc
     4981 caaagacaag ttcgctggca cctgctgtat cggtcataat ttcggttgga ttcagcccgg
     5041 tctcctgttc cagaagacct tccagcacaa agatagagtc cctcagcgtc cccggtataa
     5101 cgatgccatg aaagccggaa tactgatcgg acacaaagtt gtaccaggtg atccctctgt
     5161 tattaccaaa gtatttgcgg ttcggtccgg cattgattgt tctgactggc gtaacaaagc
     5221 gcattccatc tgcagatgcc acttctcctc caccccatat ctgtgccagt ggcagcgttg
     5281 cctgaaaatc aaccagtctg gcattagcgc tggtgatagt ttcagcccgc agatagttcg
     5341 cttttgtcca gttcagccgg tgtcgggtca gtgcaggaac atttgatctg atcagtggtt
     5401 ccagaccgat attgcaggct tcagccatca gcacggcgct gatgctgacg ggcagatcat
     5461 caactctggc actggcttca ctagcatgga aaaactcatc agcaaatccg gtatgggcgt
     5521 taatttcgag cagcaactcc gttaaatcca ccggagggag tagatcactg atcattttgc
     5581 tcagtcgttt cagactgtcc ggctcatcaa gactggcgag gggagaaatt gtcaaccggg
     5641 gcttcgggcc agaaacatcg agttcgacag cctcattttc gcaaagacgt gcagcaacct
     5701 gtctgtaacg actatcaagc tgatgaccca gagattttat tgcttcctgc gggtctgtcg
     5761 ggtgccccaa agaacgataa accttaatcc ggtttgcctg ccagtcagca ccctgtagta
     5821 atcttgcacg aggatctccc caccggttac tgccggtaac gtagacatcc ctccgcctca
     5881 gactatcctg cagtttactg agaaagcaga gcgtgtatcc cctgcgggtg atatgttttt
     5941 ccttgttaat caccagccgt ttccatgacc gactgataat ttccgttggt gcgtcgtcaa
     6001 aaaactgccg ccgtgagctg aactcccggc tgaggtagtc acaggcattc agagtggtaa
     6061 ccccggcagg tgcggatgaa aatttaacgg tattcagcag atggggcagg aaacgacgaa
     6121 cgcgcccgta ctgctccacc atttcttcat gaaaattatc gtctgagggc cgggcaattt
     6181 cacggacaag cgtgatgatt tcagccagct tttgccttgg gatgtagctg aacacctcag
     6241 cacgaatcga ttcgtccggt gtttcttctt tcagcaggta cgaacatgcg ctggcgagcg
     6301 ccaatgcaga tttatccaga tccttcagcg agcggagccg ttttttctgc ccaatctttc
     6361 tggcgtcacg gatgataacg gccagcatgg cgtccagaac gtccaatgca tcatccagcg
     6421 ccagcgtttc ccatgcaagg acaaaggcaa ccagaaccgc catccttttc tgcggtgaca
     6481 tcctggcaat attgaacacc gaagtcatac cagcataacg tgcgagattt ttcaggcgca
     6541 cagccgggag tgtactcagg ttttcagcat gcaggccaaa atcgttcaga gttttccagc
     6601 gttcaattgc ttcattaaac gccggaccac tgatggtcac agggcccttt ttcagtgatt
     6661 ccagtaaaga caggcggctg caatcagttg gccccagcag catctccagc tgtgaacgct
     6721 gttcggctga cggtatcagt gccagtttgt tccacaggcg caacgtcgcc ttttccctta
     6781 cctctgaaat caaccgggtc agcgtagtgg ctccggggag aataatacga tgttgcataa
     6841 gccaccctgt cgccagatcg aaaagcaggc caggacgttc gttgcttatc cagctccggg
     6901 tatataaaag acgggtaagg cgaaatgtcc agggccaggc aaattcacga tactgatagt
     6961 gctgacgtat cagcgctgca tgctcacggc gggtattttc cctctgaccg tattctgcaa
     7021 gaacggtgat atcacgaatc ccgagctgtc tggcggtaaa atgccggacg ccggaaggaa
     7081 tatgattcat atcggtgagg aaggtgccca gaaaacggac acatccaatt tgcagggcaa
     7141 tgcccagacg gttgtgatca cctctgcttt ttccgataaa ttccttgtct gcttcatcaa
     7201 ggtgaaaata tcgtgccagc tgaagctcat ccggttcacc ggtgaatctg ccatagcttt
     7261 cagtctgctc agtggtcaga aagtcaacgg gcatatcggc ctccctgcct gacgggcatt
     7321 tagtaacatt tttccaaccg tacgaaatgt tataaattat cggacatcgt aaaactgtta
     7381 cattaatatg tctattaaat cgtaaatttg taataataga catgagttgt ccgatattcg
     7441 atttaaggta catttttatg cgactttttg gttacgctcg ggtctcaacc agtcagcagt
     7501 ctcttgatct tcaggtcaga gcactcaaag acgcaggtgt gaaagcaaac cgtatattta
     7561 ccgataaggc atccggcagt tcaacagacc gggaagggct ggatttgctg aggatgaagg
     7621 tggaggaagg tgatgtcatt ctggttaaga agctcgaccg tcttggccgc gacactgccg
     7681 atatgatcca actgataaag gaatttgacg ctcagggcgt ggcagtccgg ttcattgatg
     7741 acgggatcag taccgacggt gatatggggc aaatggtggt caccatcctg tcggctgtgg
     7801 cacaggctga acgccggagg atcctagaac gcacgaatga gggccgacag gaagcaaagc
     7861 tgaaaggaat caaatttggc cgcaggcgta ccgtggacag gaacgtcgtg ctgacgcttc
     7921 atcagaaggg cactggtgca acggaaattg ctcatcagct cagtattgcc cgctccacgg
     7981 tttataaaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     8041 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     8101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8161 ctggtaaatg cttcaataat attgaaaaag gaagagtatg agtattcaac attttcgtgt
     8221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8401 cacttttaaa gttctgctat gtggtgcggt attatcccgt gttgacgccg ggcaagagca
     8461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8581 tgataacact gctgccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     8761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9181 ttcgttccac tgagcgtcag acccctacaa gcgtggaaca catctacgcc gcaggcgact
     9241 gcaccgacca gccgcagttc gtctatgtgg cggcagcggc cggcactcgc gccgcgatca
     9301 acatgaccgg cggtgacgcg gccctgaacc tgaccgcgat gccggccgtg gtgttcaccg
     9361 acccgcaagt ggcgaccgta ggctacagcg aggcggaagc gcaccatgac ggcatcaaaa
     9421 ctgatagtcg cacgctaacg ctggacaacg tgccgcgcgc gctcgccaac ttcgacacgc
     9481 gcggcttcat caaactggtg gttgaagaag gcagcggacg actgatcggc gtgcaggcag
     9541 tggccccgga agcgggcgaa ctgatccaga cggccgcact ggcgattcgc aaccggatga
     9601 cggtgcagga actggccgac cagttgttcc cctacctgac gatggtcgaa gggttgaagc
     9661 tcgcggcgca gaccttcaac aaggatgtga agcagctttc ctgctgcgcc gggtgaggac
     9721 aaggaggtgt gcgatgagcg cctacacggt atcgcaactg gcccataacg ctggggtgag
     9781 cgtacatatc gtgcgcgact acctggtgcg cggcttgtta cggccggtgg cctgcaccac
     9841 gggcggctac ggcgtgttcg acgatgcggc cttgcaacgg ctgtgcttcg tgcgcgcggc
     9901 cttcgaggcg ggtatcggcc tggatgccct ggcgcggctg tgccgtgcgc tcgacgcagc
     9961 ggacggcgca caagccgcag cgcagcttgc cgtgctgcgc cagttggtcg agcggcggcg
    10021 cgcggcgttg gcccatctgg acgcgcaact ggcctccatg ccagccgagc gggcgcacga
    10081 ggaggcattg ccgtgaacgc ccctgacaaa ctgccgcccg agacgcgcca acccgtttcc
    10141 ggctacctgt ggggtgcgct ggccgtgttg acctgcccct gccatctgcc gattctcgcc
    10201 gccgtgctgg ccgggacgac cgccggtgcc ttccttggcg agcattgggg tgttgccgcg
    10261 ctcgcgctga ccggcttgtt cgttctggcc gtaacgcggc tgctgcgcgc cttccggggc
    10321 ggatcatgac gagttcgcag cccgccggat ggacggcggc cgagttggcg caggcggcgg
    10381 cgcgcggaca gcttgacctg cattaccagc cgctggtcga tctgcgcgat caccggatcg
    10441 ctggcgcgga agcgttgatg cgctggcggc atccgaggct tggcctgttg ccgcccggcc
    10501 agttcctgcc gctggccgag tcgttcggcc tgatgccgga aataggcgcg tgggtgctgg
    10561 gcgaggcctg tcgccagatg cacaagtggc aaggaccggc atggcaaccg ttccgtcttg
    10621 ccatcaatgt gtccgccagc caggttgggc caacgttcga cgacgaggta aagcgggtgc
    10681 tggccgatat ggccctgccc gccgagcttc tggagatcga actgaccgaa tcggtcgcat
    10741 tcggcaatcc agccctgttc gccagtttcg acgccttgcg cgccatcggc gtgcgcttcg
    10801 ccgccgacga cttcggcacc ggctattcct gcctgcaaca tctgaaatgc tgccccatca
    10861 ccacattgaa aatcgaccaa tcctttgtcg ccaggctccc ggatgatgcc cgtgaccaaa
    10921 ctatcgtgcg ggcggtgatc cagctcgcgc acgggctggg catggatgtc attttcagaa
    10981 gacgactgca ccagttgatt gggcgtaatg gctgttgtgc agccagctcc tgacagttca
    11041 atatcagaag tgatctgcac caatctcgac tatgctcaat actcgtgtgc accaaagcga
    11101 ggtgagcatg gcgacggaca ccccacggat tccagaacaa ggcgtggcca ctctgcctga
    11161 tgaggcttgg gagcgtgcgc gccgtcgtgc ggagatcatc agtccgttgg cgcagtcgga
    11221 gacggtcggg cacgaagcgg ccgatatggc ggctcaggcg ctgggcttgt ctcggcgcca
    11281 ggtatacgtt ctgatccggc gtgcccggca aggcagcggc ctcgtgacgg atctggtgcc
    11341 cggccagtcc ggtggaggta aaggtaaggg gcgcttgccg gaaccggtcg agcgcgtcat
    11401 ccacgagcta ctgcaaaagc ggttcctgac caagcagaag cgcagcctag cggcctttca
    11461 ccgcgaagtc actcaggtgt gcaaggctca aaaactgcga gtgccggcgc gcaataccgt
    11521 ggccttacgg atcgctagcc ttgacccgcg caaggtcatc cgccggcggg aaggccagga
    11581 tgccgctcgt gacctacaag gtgtgggcgg cgagcctcct gccgtgaccg cgccgctgga
    11641 gcaggtgcag atagaccata cggtcatcga cctgatcgtg gtcgatgacc gcgaccggca
    11701 acctattggc cgcccgtacc tgaccctcgc catcgacgtg ttcacccgct gcgtgctcgg
    11761 catggtcgtc acgctggaag cgccgtctgc cgtttcggtt ggcctgtgcc tcgtgcatgt
    11821 cgcctgcgac aagcgccctt ggctggaagg actgaacgtg gaaatggatt ggcagatgag
    11881 cggcaagccc ttgctgctct acctagacaa cgcggccgag ttcaagagcg aggccctgcg
    11941 ccggggttgc gagcagcatg gcatccggct ggactatcgc ccgctgggac agccgcacta
    12001 tggcggcatc gtggaacgga tcatcggcac ggcgatgcag atgattcacg acgaactgcc
    12061 gggaacgacc ttctccaacc ctgaccagcg cggcgactac gattccgaaa acaaggccgc
    12121 cctgacgctg cgcgagctag agcgctggct cacattggcg gtcggcacct accacggttc
    12181 ggtgcacaac ggcctgctcc aaccgccggc cgcgcgctgg gccgaggccg tggcgcgtgt
    12241 cggcgtaccg gccgtcgtca cacgcgctac ttcgttcctg gtcgattttc tgccgatcct
    12301 ccggcgcacg ctgacccgca ccggctttgt catcgaccac atccactact acgccgatgg
    12361 gcactgttgc aaatagtcgg tggtgataaa cttatcatcc ccttttgctg atggagctgc
    12421 acatgaaccc attcaaaggc cggcattttc agcgtgacat cattctgtgg gccgtacgct
    12481 ggtactgcaa atacggcatc agttaccgtg agctgcagga gatgctggct gaacgcggag
    12541 tgaatgtcga tcactccacg atttaccgct gggttcagcg ttatgcgcct gaaatggaaa
    12601 aacggctgcg ctggtactgg cgtaaccctt ccgatctttg cccgtggcac atggatgaaa
    12661 cctacgtgaa ggtcaatggc cgctgggcgt atctgtaccg ggccgtcgac agccggggcc
    12721 gcactgtcga tttttatatc tcctcccgtc gtaacagcaa agctgcatac cggtttctgg
    12781 gtaaaatcct caacaacgtg aagaagtggc agatcccgcg attcatcaac acggataaag
    12841 cgcccgccta tggtcgcgcg cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg
    12901 ttgaacaccg acagattaag taccggaaca acgtgattga atgcgatcat ggcaaactga
    12961 aacggataat cggcgccacg ctgggattta aatccatgaa gacggcttac gccaccatca
    13021 aaggtattga ggtgatgcgt gcactacgca aaggccaggc ctcagcattt tattatggtg
    13081 atcccctggg cgaaatgcgc ctggtaagca gagtttttga aatgtaaggc ctttgaataa
    13141 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccc atttttggga tattaccgcg
    13201 gtatcggccc tcgacaaggt agtgattaaa ttccgccgcg aggggactga ggttgagttg
    13261 attggcctta acgaagccag tgccaccata gtcgataaat ttggggtaca cgataagcca
    13321 gaagaagttg aaaaaatgat gtctggtcac taagccatta gcaacacaac aacaaaggaa
    13381 taaaacgatg acaaatgtga ttgcttgtat tgacggttca aatgtgacat ccgctgtatg
    13441 tgatgccagt ggctgggccg cctttcaact gaatgcccct gtaatcctag gtgatgctgc
    13501 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
    13561 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
    13621 gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca
    13681 ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat
    13741 gccgggcaac cgcccgcatt atgggcgttg gcctcaacac gattttccgc catttaaaaa
    13801 actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc
    13861 ggaaatggac gaacagtggg gatacgtcgg ggctaaatcg cgccagcgct ggctgtttta
    13921 cgcgtatgac aggctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc
    13981 gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga
    14041 tggctggccg ctgtatgaat cccgcctgaa gggaaagcag cacgtaatca gcaagcgata
    14101 tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg
    14161 gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct
    14221 gaacataaaa cactatcaat aagttggagt cattaccctc ccctcaaagc catatcacgt
    14281 acgttaatcc tgacttcatt aaaatcagtg gtttcactga ggaagaacta ttaggccagc
    14341 ctcacaacat cgtaagacac ccagatatgc cgcctgctgc atttgagcat atgtggagta
    14401 cattaaaatc tggccgctca tggatggggc tagtaaaaaa tcgctgtaaa aatggcgacc
    14461 actattgggt aagtgcttat gtaacgccaa tagctaagaa tggttcgatt gttgaatacc
    14521 agtctgtaag gaccaagcct gaacctgagc aggttttggc tgcggaaaaa ttatatgctc
    14581 aattgagaag cgggaaggcc gcgaggccga aattggctgc tagcttttcc gtgaaaatac
    14641 tcttgctcat atggggtagt attatatcaa gcgcaatggc tgccggcatg cttactgata
    14701 catcaataag cagcttattg ttagccactt taatgtcagg aagcttaagc tctgttagtg
    14761 ttttggctat tctctctcct cttggaagac tggttgaaag agccaggaat atttccaata
    14821 acccattaag tcaatccctc tacactgggc gcaccgatga gtttggccaa atagagtttg
    14881 ctttacgaat gatgcaagct gaaacaggcg ccatagtagg tcgcataggt gatgcatcaa
    14941 atcggcttag cgaacacacc cgaggcctac taaaggatat tgagtcaagc aatgtactta
    15001 cagttgagca gcaggcagag acagatcaaa tagcaacggc agtaaaccaa atggtggcaa
    15061 gcattcaaga ggttgcgagc aatgcacagc atgctgcaga tgcggccgga agagcagaca
    15121 ctgagacggc atctggccag cgtctggtag cccacacaag ccagtcaatc actgcccttg
    15181 aaggtgaaat taggcaagcc actcaggtta ttcatgagct tgaaggtcaa agtaacgaga
    15241 tatcaaaagt tcttgacgtt atacgaggga tcgccgagca aacgaatttg ttggcactca
    15301 acgcagcaat tgaggccgcg cgtgctggtg agcaggggcg tggttttgct gttgtcgccg
    15361 atgaggttcg cagtcttgct gctcgcacac agcaatcgac aacggatatt caaagcatga
    15421 tcagcgctct acaagagcga gcgcaatccg ctgttacagt catggagcaa agtagtcggc
    15481 aagcgcacac gagtgtagct cacgcagagg aagcagctac agctcttgat ggaattggcc
    15541 aacgcgttaa cgaaattacc gacatgaacg cgcaaatagc gactgcggtc gagcagcagg
    15601 gagcagtaag tgaagacata aaccgcagta ttatcaatat acgcgatgct gctgatacca
    15661 atgtacagac cgggcagaat aatttgcaaa gtgcgaaatc tgtcgctcag ttaactagcg
    15721 ctctgagcga actggcaaaa cagttttggg aaaaacgagg ataacgcttt tcagtatctc
    15781 gacagggata acttacttta ccatcggtta tcccttttag ccgcacaaat tttagccatg
    15841 gcttttcagg aagtcagggc tatcagaatg gccttagaaa gcctagtcaa agagctgtca
    15901 cgagaacacc gttagcttag cgtacgattt tttccgaatt ctgcggttcc cccaaagagg
    15961 ctgcacagcg gcgattcttg ccctaaaata gcgtaaaaaa cagcggcaaa gcgcgataac
    16021 aacaccgggt gtcgccaggg ccgtagcctg ggtggtgacg cctggtgtga aaaaatggta
    16081 aggggagagc ggtgattcca atagaacgac atcagcgtat tttagccctg gtggagcagc
    16141 gtggggcggt aagcattaac gagctgacgg agatcctcgg cgtgtcccat atgaccatcc
    16201 gtcgggacgt cagtaaactg gaggagcagg ggctgctggt cagcgtctcg ggcggcgtac
    16261 gcgccgtcag ccggctggcc gcggaaccca gtcatctggt gaaaagcacg ctgcagagtg
    16321 aggagaaaca ggcgatcggc gcgctggcgg cgagtcatat cgctaaaaac agttgcatct
    16381 atctggatgc cgggactacc accctggcgc tggcgcgggc gatcctcgac cggaacgatc
    16441 tccaggtggt caccaatgat tttgagataa cccagctgct gatcgatgcc agccagtgcg
    16501 gcgtgatcca caccggcggc actctgtgcc gggagaaccg ctcctgcgtg ggcgaatcgg
    16561 cggcgcgtac gttacgccac ctggctatcg atacggcctt tatctccgcc agcggctggg
    16621 acagtcgcgg gatattcacc cctgatgaga acaaggttac cgtcaaggag accgtcagcc
    16681 aggtcagcgc gagaagcatc ctgctgtgcg acagttcgaa atacaatcag gtggccacgt
    16741 ttatggcgtt acctttgacc cggttcacca ccatcattac cgaccggcat ctttccgatg
    16801 ccgccgccag tcatatcgcc cggcacgcct gcgaggtgct gcgggccgga taacgcgcgc
    16861 ggccaccgcc gggttagcgt tgccagtgct cgatcagcgc cgcgccgatc ccggcgattt
    16921 gctgatttcg ctcggccatg ctcgccggcg tatcccgcag ataaatcacc acaatgcgct
    16981 ctgctttgtt attcgggcca agcagggcga caatcccgcg cgcaccccgc ttgctagctc
    17041 cggtcttatc ggcgataaac cagcccgccg gcagcacgga gcggatcaac ggtccggcga
    17101 cccgatcgtc caccatccac tgcagcagct gccgttgcga acgggcgctc agacgctggc
    17161 tggtcagcag cttgcgcagg gtcgcggcca tgctggccgg ggtagtggtg tcgcgggcgt
    17221 cgccgggaag cgcctcattc agttccgttt cccagcggtc aaggcgggtg acgttgtcgc
    17281 cgatctggcg caaaaaggca gtcaatcctg cggggccgcc gacggtggcc agcagcagat
    17341 tggcggcgct gttatcgctc atggtaatgg cggcggcgca gagttcgccg accgtcatgc
    17401 cgtcggcaag gtgtttttcg ctgaccggcg agtagtccac cagatcctgc tggcgatagt
    17461 ggatctttcg ctccagctgt tcgtcaccgg catccacccg cgccagcact gcgccgcaga
    17521 gcactacttt aaaggtgctc atcatgggaa agcgttcatc ggcgcgccag gcggtcagcg
    17581 tgcggccgct ggccagatcc atttctatca tgcctacgcg gcccgacagc tggctttcgc
    17641 tttgtttaat ttgctcaagc ggctgcgggc tggcgtgtac cgccagcggc agggtggcta
    17701 acagggagat aatacacagg cgaatataac gcataaccac aatacatcct tgagtgaagg
    17761 ccgataaagg cgagtaaaga agcgacaaat aagaataacc cggcgttggc actgttgcaa
    17821 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
    17881 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
    17941 acggcatcag ttaccgtgag ctgcaggaga tgctggctga acgcggagtg aatgtcgatc
    18001 actccacgat ttaccgctgg gttcagcgtt atgcgcctga aatggaaaaa cggctgcgct
    18061 ggtactggcg taacccttcc gatctttgcc cgtggcacat ggatgaaacc tacgtgaagg
    18121 tcaatggccg ctgggcgtat ctgtaccggg ccgtcgacag ccggggccgc actgtcgatt
    18181 tttatctctc ctcccgtcgt aacagcaaag ctgcataccg gtttctgggt aaaatcctca
    18241 acaacgtgaa gaagtggcag atcccgcgat tcatcaacac ggataaagcg cccgcctatg
    18301 gtcgcgcgct tgctctgctc aaacgcgaag gccggtgccc gtctgacgtt gaacaccgac
    18361 agattaagta ccggaacaac gtgattgaat gcgatcatgg caaactgaaa cggataatcg
    18421 gcgccacgct gggatttaaa tccatgaaga cggcttacgc caccatcaaa ggtattgagg
    18481 tgatgcgtgc actacgcaaa ggccaggcct cagcatttta ttatggtgat cccctgggcg
    18541 aaatgcgcct ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg
    18601 cctcatcgct aactttgcaa cagtgcccta tttttatagg ttaatgtcat gataataatg
    18661 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    18721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg gtaaatgctt
    18781 caataatatt gaaaaaggaa gagtatgagt attcaacatt ttcgtgtcgc ccttattccc
    18841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    18901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    18961 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    19021 ctgctatgtg gtgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
    19081 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    19141 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgct
    19201 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    19261 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    19321 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
    19381 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    19441 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    19501 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    19561 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    19621 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    19681 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    19741 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    19801 gcgtcagacc cct
//
