LOCUS       NZ_LT985246             4772 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 720 plasmid RCS48_pIII, complete sequence.
ACCESSION   NZ_LT985246
VERSION     NZ_LT985246.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588835
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289525.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:30:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 118
            CDSs (total)                      :: 118
            Genes (coding)                    :: 106
            CDSs (with protein)               :: 106
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985246.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="720"
                     /db_xref="taxon:562"
                     /plasmid="RCS48_pIII"
     gene            1500..2051
                     /locus_tag="GWB39_RS00005"
                     /old_locus_tag="RCS48_PIII0003"
     CDS             1500..2051
                     /locus_tag="GWB39_RS00005"
                     /old_locus_tag="RCS48_PIII0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(2250..2465)
                     /locus_tag="GWB39_RS00010"
     CDS             complement(2250..2465)
                     /locus_tag="GWB39_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137420.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMFGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2469..2954)
                     /locus_tag="GWB39_RS00015"
                     /old_locus_tag="RCS48_PIII0006"
     CDS             complement(2469..2954)
                     /locus_tag="GWB39_RS00015"
                     /old_locus_tag="RCS48_PIII0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_089624851.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKVAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(2967..3668)
                     /locus_tag="GWB39_RS00020"
                     /old_locus_tag="RCS48_PIII0007"
     CDS             complement(2967..3668)
                     /locus_tag="GWB39_RS00020"
                     /old_locus_tag="RCS48_PIII0007"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_159373672.1"
                     /translation="MPCWKRVQTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGK
                     PEEVRELIDASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWV
                     EHQDKGRLELNFLIPNMELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENR
                     RALVTPSALPKTKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSIS
                     IADPDGGETSD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3631..3954)
                     /locus_tag="GWB39_RS00025"
                     /old_locus_tag="RCS48_PIII0008"
     CDS             complement(3631..3954)
                     /locus_tag="GWB39_RS00025"
                     /old_locus_tag="RCS48_PIII0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957080.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLAEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4592..4675
                     /locus_tag="GWB39_RS00635"
     CDS             4592..4675
                     /locus_tag="GWB39_RS00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058652899.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="hypothetical protein"
     Typing          complement(4056..4243)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.864"
                     /coverage="87.0"
                     /evalue="5.83e-49"
     Typing          4189..4701
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.829"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2147..2994)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.753"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3232..3641)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.401"
                     /coverage="83.0"
                     /evalue="3.5000000000000004e-70"
ORIGIN
        1 tatttggtga catgtctcgc acaagactgt taccacggtt agagaatctg ccaggattaa
       61 ctaaccttcg aaaaaaccac ctgccagggt ggttttttcg tttctgagca agagactacg
      121 acgagccact gatgatctca agtgttcatt tctgatattc acagcagaat aactgttagg
      181 atatagggta tctatagcca ggagcttcaa gaaatccaga cacagcaatg gattccaggg
      241 ttatcgaaaa aaattagtat ataggttgca accaaaatgt tggttgcaaa tctacatcac
      301 gatgtttatg attattgtac tgcaagagca ttcgcagggg gcatcaacca gtaataggcc
      361 ttctgtgccg ggcagtcaga tggctggtca tctgccgggt agttacgaca agcgtcggtt
      421 tctgaaccct atgtgctaag gaaatgctgc gactaccaac gaattcataa agggcgcttt
      481 ttagcgccct atttttttat tcaatatgac caaccaaagt accgtgcgcc gtccttcgtg
      541 tgtacgcagt cacaacgaca tagatccaac ctgactcagt ttcaggcgct tctctgggct
      601 caagaattac gatgcctacc gacgacttta gcactgcagg ccgatgttta ccgcttgtac
      661 tgttgaactc gcaatatatt ggtacgccac gacgaataat atcccggaca aaacgagcca
      721 catcattaac ggtcgcatat cccagcttaa ccagttcctg ctcgtgctca gcccagatat
      781 ggcgaacacc aaagccccgg ttagggccag tatgcctacc aacacgcaag aagatgtcgc
      841 cagcaggaat aacatcacct ttagaattaa tttcagggac acggccatag caatcaagcc
      901 catcagggtg agctatagcg ctgttaggtg aaccaactac accgtaattg tttggagcag
      961 gcacaaaacg atccttattg agtagagtcc gcatatggta ccagagagga aacaagcgga
     1021 aggagcccaa gaaaaaagca gagacgataa aaagaatcct tatggattat ttttttgtcc
     1081 tttcatcctt ggcgcataag gatatcacac ctgaaaatgt gacgtgcgaa tcgccctagc
     1141 agctagattt accataacat cccttgcccg caccgctccg gacgggttca gcgtgaaagc
     1201 ggcgttagcc cggtaaaacc ggcagtaaac tccccgctaa accgcgcatt ctgcgcctga
     1261 taaatttcac gcggctgcat agccatgcag ccacgtgaat ggctcatgct ttcaatgtat
     1321 atcctcgtcc aagcccctgt tttaaccggt ttatcaaaaa aacaaacagg aagccttatc
     1381 actaacaata aaagaggtag ggggccgaat tttatcgcat aaaaaacatt tacaacacga
     1441 attctatcgt atagatttca taaaaaaatg aaattgactc acctattaag ggtttctata
     1501 tgaaagatat gaaagctgtt gtggttttta ccgggaagga tttgaacata atgagaactg
     1561 agggggggtc aggctactgg catgccagaa cagacaggct caacgatgcc gattacctca
     1621 tagcagtaag aaacagaagg gaaacgtggg cagtaaaaga cctggaacac ggcacagcat
     1681 tcctgatcgc caaaattacc gggtgtttta aaagccctga ttacgacgac aggaacgtca
     1741 taacctttga tgaatacgca gaaattcaca ctccaaaagc atggaaaatg ctaaccgatg
     1801 gtcagcgcta tccggttgct tacctgagcg cacaggaagc ttttttacgt attggggtaa
     1861 caccggaaca actggaatgg aagaaattcc atccttcatc cccatcggtt cctaatacag
     1921 tgattcctgg tctggctgaa gagaaaacag aaaaactctc ccttaacgag gcaattgaaa
     1981 gagccaaaaa ggatattagc aacgcaacag ggattgatag ctcagccata acaataagca
     2041 tcaagatctg atgaagcgtg aacaacaaga tagcagcctc atgttctaga actgaaccta
     2101 tgcgccagaa acagcaagcc catgcttttc cggcgcacag attcaaagtg acggaccgtt
     2161 gtacgttcgc tgtcgctgag atcgttccag ctccagcgct ttctgatgct gcgccgcccg
     2221 ttcctgctca cgatgtgcct ctatcacgtt cagccttgtg acaaccggcg cagctgctcg
     2281 ctcaaactct gcacctgcct gctcaagccc gtgacgcgct cgctcagcgc cgcgttctcc
     2341 cgttgcataa gaccaaacat cgtccgccat tccgcgaagg cgttctccca ctcgtccagc
     2401 cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt ttccagctcc
     2461 gtcatgtgct atttccccgc cagtatcatc cagctcgaat cctctccgaa ccgtcccgct
     2521 tccgggttca ccctcacgca gcggcgtctc tgctctccgc aggtcgagag ctgcacgccg
     2581 ctgttcctct cggacagcat ggcctgcgtg ctcttctgct cccggatgat cgtataattg
     2641 tcgagtatct gctgcccctg ccaccataga atgcttgcgc ccgatgctgt cagcagtgta
     2701 gacaccagga cgatggtcag ccacgtctgg ctgaccatac gcaccatgcc tttcgtgcgc
     2761 tggctcatgg ctgaggacag cttccggtcg tggtcgagga tggcggcgct gattctcttc
     2821 tcgctttcgc tcagttccgc tctgacagac ttctcgtgct cgctgaatgc gactttcagc
     2881 atctcgccgg tactctgctg ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt
     2941 aaaagactgt tcatagatgg ctcccttcag tcggatgttt cgcccccgtc cgggtcggca
     3001 atgctgatgc tgctttttgt ggtgcgcacc acctcaaaac ctgcgctttc tagcgcctca
     3061 gtgacgtcct ggcgggtttt cagctccccg gacgaggcta aggccagtaa accccgtgta
     3121 attgcctgag cggcttcctg cttcgttttc ggcagcgcgg acggcgtgac cagcgcccgc
     3181 cggttttcag gtgcgttcgg gtcatgcagc cccagcctgc cgttcacgat ggtctgccag
     3241 gcatcaatgc gcggacggtc ggcgcggtcg taatacggct ggagacgcct gcctgtcagc
     3301 agctccatgt tcgggatcag gaagttcagc tccagccgcc ccttatcctg gtgctcaacc
     3361 cacagcacgc tgtactggtc tttatcgagt ccgggcatca gaacccgttc aaagctcgcc
     3421 atcagctttt cgcgctgccc gggcggtaaa tccttttcgg caaaggacag gacgccggat
     3481 gtgtactttt ttgcgtaggg cgaggcgtcg ataagctccc tgacttcttc cggcttcccc
     3541 tgcagaacgg tggcgccgtc gcgctgtcgg tctttcccca gcagataatc caccggaccg
     3601 gcgccaccgc cgcgccctcg cgggtgaaac ttaacgatca tcgtctgcac ccttttccag
     3661 cacggcatgc cgcagccgct cgagtccggc atctatggcc atcagcgcgg cgacgacctg
     3721 cacgcggtcg tgcccgctgc cgccaccggc gttaacctgc cgggcaatct ggttgaggtt
     3781 attccccatg ccggcaagct gacgaagcag cgccggcgag atcgagggaa gcttgccggc
     3841 gcgggcgggt ttctcggcca ggcacgtctg gcgcatccag gctgcaagct gtttaccgtc
     3901 gcagcgttcc agcagacggc ggtgctcatc ttcggtcacc cacatggtga gcatcttgtt
     3961 ccgtttctct gacaaaccga tctcctgata acgccgggac gggcgggaaa tcttttccag
     4021 gtcggcgcgg tgttggacaa gccgcaggcg cgtcagtggt tttagcaggt ttcggggcgc
     4081 agccctgaac cagtcacgta gcgctagcgg agtgtatact ggctcaatat gttacggcgg
     4141 agtaacacgt aatgaaagtg cttcatgtgg caggggaaaa atgcagctgc agggcgtaca
     4201 ggagaaaatg gaggttacgg tgatatgacg cctcctcgct cactcggtcg cttccgctcc
     4261 tgacgttcgg ctgcggcggg cgttacctgc aaacggataa gagcaaaggc atacggcatg
     4321 gcaagcagga caggaaaagg atgcggcgct gccgtttttc cataggctcc gcccccctga
     4381 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gacttaaaga
     4441 taccaggcgt ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     4501 accggtgtca ttccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcagttc
     4561 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     4621 ctgcgccttg tccggtaact atcgtcttga gtccaacccg gatagacacg actaatcgcc
     4681 attggcaacg gccactggta agcaggatgt gcgagagatg taacgagagt tcttgaagtg
     4741 gcggcctaac tgaggctaca ctgaaaggac ag
//
