LOCUS       NZ_LT985237             6647 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 641 plasmid RCS33_pII, complete sequence.
ACCESSION   NZ_LT985237
VERSION     NZ_LT985237.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588823
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289435.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:30:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 111
            CDSs (total)                      :: 111
            Genes (coding)                    :: 108
            CDSs (with protein)               :: 108
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985237.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="641"
                     /db_xref="taxon:562"
                     /plasmid="RCS33_pII"
     gene            232..390
                     /locus_tag="GO935_RS00590"
     CDS             232..390
                     /locus_tag="GO935_RS00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            888..1115
                     /locus_tag="GO935_RS00500"
                     /old_locus_tag="RCS33_PIICDS774D"
     CDS             888..1115
                     /locus_tag="GO935_RS00500"
                     /old_locus_tag="RCS33_PIICDS774D"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1112..2680)
                     /gene="cea"
                     /locus_tag="GO935_RS00505"
                     /old_locus_tag="RCS33_PII0001"
     CDS             complement(1112..2680)
                     /gene="cea"
                     /locus_tag="GO935_RS00505"
                     /old_locus_tag="RCS33_PII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2940..3377
                     /locus_tag="GO935_RS00510"
                     /old_locus_tag="RCS33_PII0002"
     CDS             2940..3377
                     /locus_tag="GO935_RS00510"
                     /old_locus_tag="RCS33_PII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3458..3877
                     /locus_tag="GO935_RS00515"
                     /old_locus_tag="RCS33_PII0003"
     CDS             3458..3877
                     /locus_tag="GO935_RS00515"
                     /old_locus_tag="RCS33_PII0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4195..4428)
                     /locus_tag="GO935_RS00520"
     CDS             complement(4195..4428)
                     /locus_tag="GO935_RS00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4435..4953)
                     /locus_tag="GO935_RS00525"
                     /old_locus_tag="RCS33_PIICDS4435R"
     CDS             complement(4435..4953)
                     /locus_tag="GO935_RS00525"
                     /old_locus_tag="RCS33_PIICDS4435R"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4991..5689)
                     /locus_tag="GO935_RS00595"
                     /pseudo=""
     CDS             complement(<4991..5689)
                     /locus_tag="GO935_RS00595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5631..5954)
                     /locus_tag="GO935_RS00535"
                     /old_locus_tag="RCS33_PII0006"
     CDS             complement(5631..5954)
                     /locus_tag="GO935_RS00535"
                     /old_locus_tag="RCS33_PII0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6018..6209
                     /locus_tag="GO935_RS00540"
                     /old_locus_tag="RCS33_PII0007"
     CDS             6018..6209
                     /locus_tag="GO935_RS00540"
                     /old_locus_tag="RCS33_PII0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6295..6382)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..341
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.067"
                     /coverage="83.0"
                     /evalue="5.96e-161"
     Typing          6393..6647
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.996"
                     /coverage="100.0"
                     /evalue="3.25e-100"
     Typing          complement(4092..5641)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacgagcatc acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa
       61 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      181 cgctgttggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      301 gtaaggcacg ccttaacgcc actggcagca gccactggta accggattag cagagcgatg
      361 atggcacaaa cggtgctaca gagttcttga agtagtggcc cgactacggc tacactagaa
      421 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      481 gctcttgatc cggcaaacaa accaccgttg gtagcggtgg tttttttgtt tgcaagcagc
      541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc
      601 gatccccgtc agtttagaag aggaggatgg tgcgatggtc cctccctgaa catcacgtat
      661 atagttagcc tgacatccaa caaggaggtt tatcgcgaat attcccacaa aaaatctttt
      721 cctcataact cgatccttat aaaatgaaaa gaatatatgg cgaggtttaa tttatgagct
      781 taagatacta cataaaaaat attttatttg gcctgtactg cacacttata tatatatacc
      841 ttataacaaa aaacagcgaa gggtattatt tccttgtgtc agataagatg ctatatgcaa
      901 tagtgataag cactattcta tgtccatatt caaaatatgc tattgaatac atagctttta
      961 acttcataaa gaaagatttt ttcgaaagaa gaaaaaacct aaataacgcc cccgtagcaa
     1021 aattaaacct atttatgcta tataatctac tttgtttggt cctagcaatc ccatttggat
     1081 tgctaggact ttttatatca ataaagaata attaaatccc taacacctca tttatagtat
     1141 taagtttatt cttatcaata taggagcata gaattcctgt aacaatagca ataccccaaa
     1201 tacctaatgt agttccagca agcaagctaa aaagtaaagc aacaacataa ctcacccctg
     1261 catctgcagc tttcttctct aatgtaagaa atagtggctt ccagtcacct gtatccttaa
     1321 tttttaggat atcagatacc acatcatatc caaaagaaac atgccccgta atcttcaagt
     1381 acttggcaaa ctgatctaaa tgtttagccc agtcatcata cttcaccgat gccaacgcat
     1441 taaaaatagc atcacgatcg gctttgctga atttcttatt taaaacatcc ttgtattttt
     1501 caaaagcagc gagagcttca ttcacattgc cgattttctt acctttagac ttatcagcaa
     1561 gttcctgtgc cattttcgaa tatttttcac catatttttc agtcagcgtt tgataaaagc
     1621 taactgttgc atcaacagca tccttaatct gtgaattaag gagattattc tgtgcttttt
     1681 tcaaattttc ttcagcttca tgaacacgag cgataccggc attacgatta ttactgacct
     1741 gagaaatagc cttctggatc tgagttatat cagcatttat ccggttaata cgtgtttctg
     1801 atgctgttac ctgtttttgt ttttcttctc taatcttacc ggccccaacc cgtcgtctgg
     1861 ttgcttcaaa aaaaggacgg ttctgaagcg gatcattggc tcttggtgat agttttttga
     1921 ccagctcatc cagttcttta tatttagcgg atgcctgagc cagttcattt cgttttccag
     1981 cgagcgtttt catttctgca tcacgggcat ggatactgga gcttaaacga gaattgagag
     2041 tcttaatctc tccatccatt ttcaccactt cagattgtgc agcagaaagt tttttttggg
     2101 cgatctcaac agctttagct tcttcactca atgcagccag tcgtttctct tcagcttcag
     2161 ccagtttcaa ctggcgttct gtttcagcct tctcccgttc aatctcttta cgtcgttgtt
     2221 ctgcttcctg aaaagccttt tctgctgctt ccgcttcttt acgggctttt tcttctgctt
     2281 tcgcaaggcg caaacgctct gcttccgcct gcatagctgc attattagca tgagcaagct
     2341 ctgttgctga aggcgtacgt gaggcattgt gacgaagagc ctcattcacg atatccttca
     2401 ggcgctgagt cagcgcatcc ctgtttgcct ttgctttcgc ctgtgcttcc gctgcagctt
     2461 ttgcccgggc agcctgctct gcctgtgttt tctttaattg agcagtagac catttagcag
     2521 ttgcatgaat agctgcagaa ctttcacttt tactgcctcc ttttccacct ccgccgccag
     2581 agccactccc gtcaggagta ccattcaaaa gagtaataat tacctgtccc ttatcatcat
     2641 aaggaacacc atctttatag tacgctaccg cggtttccat tataaaatcc tctttgactt
     2701 ttaaaacaat aagttaaaaa taaatactgt acatataacc actggtttta tatacagcat
     2761 aaaagctacg ccgctgcatt ttccctgtca agactgtgga cttccatttt tgtgaaaacg
     2821 atcaaaaaaa cagtctttca caccacgcgc tattctcgcc cgatgccaca aaaaccagca
     2881 caaacattac cgttctcaga cctcattatg ttttactgaa actatgagat gagacatcta
     2941 tgggacactg tcactttatg gcatggcaca cactccggga cgcactaaaa atgacaggca
     3001 gatcgcgttc acagttttac cgtgatatgc gcgcaggcct tgtcagttac cgtaccggca
     3061 gggacggacg acgggagttt gaaaccagtg aactgatccg ggcatacggc gaattaaagc
     3121 agaatgagac accagaaagg cacagtgagg gacatgcaga aaatccacat gatcagcaga
     3181 cagaacgcat tctccgggag ctgaatgagc tgaaacaatg cctgacgctg atgcttgagg
     3241 ataaacaggc acaggatatg gatcgcagac gccaggaagc agaacgggaa cagctacaaa
     3301 atgagatagc ccagctcagg caggcactgg aactggaaaa gaaacgggga ttctggtcca
     3361 ggttgttcgg tcgctgaacg ctgtcagaga ctgatgataa aatagtcttc ggataataac
     3421 tcaccgagaa taaatacttt aaggtaggga gacactcatg agacgtaccg gaaacaaact
     3481 ttgtcttatc gccatgataa cagcaacagt agctctcaca gcctgtaccc caaagggcag
     3541 cgtggaacaa catacccggc attacgtata tgcttctgat gacggttttg atcccaactt
     3601 ttccacccaa aaagccgaca caacacgaat gatggtgcct ttttttcggc agttctggga
     3661 tatgggagct aaagataaag cgacaggaaa atcacggagt gatgtgcaac aacgcattca
     3721 gcagtttcac agccaggaat ttttaaactc actccggggc acaactcaat ttgcgggtac
     3781 tgattaccgc agcaaagacc ttaccccgaa aaaatccagg ctgctggctg acacgatttc
     3841 tgcggtttat ctcgatggct acgagggcag acagtaagtg gatttaccat aatcccttaa
     3901 ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca gcagacaggt aaaaatggca
     3961 acaaaccacc cgaaaaactg ccgcgatcgc gcctgataaa ttttaaccgt atgaatacct
     4021 atgcaaccag agggtacagg ccacattacc cccacttaat ccactgaagc tgccattttt
     4081 catggtttca ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt tccggctgac
     4141 gctcccgttc cagggataac acatgttcgc gctcctgtat cagccgttcc tctcttatct
     4201 ccagttctcg ctgtataact ggctcaagcg ttctgtctgc tcgctcaagt gctgcacctg
     4261 ctgactcaac tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa
     4321 catcttctgc aattccacga aggcgctctc ccattcgctc agccgctgca tatagtcctg
     4381 ttgcagctgc tctaaggcgt tcagcaaatg tgtttccagc tctgtcactc tgtgtcactc
     4441 cttcagatgt acccactctt tcccctgaaa gggaatcacc tccgctgatt tcccgtacgg
     4501 aaggacaagg aatttcctgt tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt
     4561 cagtttctgc aatgtctctt cctgcttcct gatttcttcc aggttcgcct gtatcctccc
     4621 tccaagatac cagagcgtcc cgccactcgc ggtaaacagg agaaagacta tccccagtaa
     4681 catcatgccc gtattccctg ccagctttaa cacgtccctc ctgtgctgca tcatcgcctc
     4741 tttcacccct tcccggtgtt tttccagcga ttcctctgtc gaggctgtga acagggctat
     4801 agcgtctctg attttcgtct cgtttgatgt cacagcctcg cttacagatt cgccgagcct
     4861 cctgaactcg ttgttcagca ttttctctgt agattcggct ctctctttca gctttttctc
     4921 gaactccgcg cccgtctgca aaagattgct cataaaatgc tcctttcagc ctgatattct
     4981 tcccgccgtt cggatctgca atgctgatac tgcttcgcgt caccctgacc acttccagcc
     5041 ccgcctcagt gagcgcctga atcacatcct gacggccttt tatctctccg gcatggtaaa
     5101 gtgcatctat acctcgcgtg acgccctcag caagcgcctg tttcgtttca ggcaggttat
     5161 cagggagtgt cagcgtcctg cggttctccg gggcgttcgg gtcatgcagc ccgtaatggt
     5221 gattcaccag cgtctgccag gcatcaattc taggcctgtc tgcgcggtcg tagtacggct
     5281 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     5341 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     5401 gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggcggcagc tccttctccg
     5461 cgaacgacag aacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     5521 ggacttcttc cggattaccc tgaagcaccg ttgcgccttc gcggttacgc tccctcccca
     5581 gcaggtaatc aaccggacca ctgccaccac cttttcccct ggcatgaaat ttaactatca
     5641 tcccgcgccc cctgttccct gacagccaga cgcagccggc gcagctcatc cccgatggcc
     5701 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcacctta
     5761 cgggctgtct gattcaggtt atttccgatg gcggccagct gacgcagtaa cggcggtgcc
     5821 agtgtcggca gttttccgga acgggcaacc ggctccccca ggcagacccg ccgcatccat
     5881 accgccagtt gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     5941 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta ccccatgaac agaaatcccc
     6001 cttacacgga ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     6061 tcagaagcca gacattaacg ctgctggaga agctcaacga actggacgca gatgaacagg
     6121 ccgatatttg tgaatcgctt cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac
     6181 gtttcgggga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6241 gtctgtgagc ggatgccggg agctgacaag cccgtcaggg cgcgtcagca ggttttagcg
     6301 ggtgtcgggg cgcagccctg acccagtcac gtagcgatag cggagtgtat actggcttaa
     6361 ccatgcggca tcagtgcgga ttgtatgaaa agtacgccat gccgggtgtg aaatgccgca
     6421 cagatgcgta aggagaaaat gcacgtccag gcgcttttcc gcttcctcgc tcactgactc
     6481 gctacgctcg gtcgttcgac tgcggcgagc ggtactgact cacacaaaaa cggtaacaca
     6541 gttatccaca gaatcagggg ataaggccgg aaagaacatg tgagcaaaag accaggaaca
     6601 ggaagaaggc cacgtagcag gcgtttttcc ataggctccg cccccct
//
