LOCUS       NZ_LT985230            10551 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 730 plasmid RCS37_pI, complete sequence.
ACCESSION   NZ_LT985230
VERSION     NZ_LT985230.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588826
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289415.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:12:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 139
            CDSs (total)                      :: 139
            Genes (coding)                    :: 130
            CDSs (with protein)               :: 130
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985230.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="730"
                     /db_xref="taxon:562"
                     /plasmid="RCS37_pI"
     gene            complement(23..265)
                     /locus_tag="GO913_RS00005"
                     /old_locus_tag="RCS37_PI0001"
     CDS             complement(23..265)
                     /locus_tag="GO913_RS00005"
                     /old_locus_tag="RCS37_PI0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052895112.1"
                     /translation="MALVRSGNKSKIFEADKKFNSVFDTEYQPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPEQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            452..769
                     /locus_tag="GO913_RS00010"
                     /old_locus_tag="RCS37_PICDS452D"
     CDS             452..769
                     /locus_tag="GO913_RS00010"
                     /old_locus_tag="RCS37_PICDS452D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001183575.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGIEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            973..1329
                     /locus_tag="GO913_RS00015"
                     /old_locus_tag="RCS37_PI0002"
     CDS             973..1329
                     /locus_tag="GO913_RS00015"
                     /old_locus_tag="RCS37_PI0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_052895113.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDNSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1372..1770)
                     /locus_tag="GO913_RS00685"
     CDS             complement(1372..1770)
                     /locus_tag="GO913_RS00685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052895114.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARYV
                     GGATEFLKWWPNYGYTGRTVFGICALLKPVTRLSSSPTDLTFEKPPPQAVFSFTEQEIT
                     RTEHGSQEDRKKKVNMSVFIPQKEGFYK"
                     /name="hypothetical protein"
     gene            complement(2440..2631)
                     /locus_tag="GO913_RS00020"
                     /old_locus_tag="RCS37_PI0003"
     CDS             complement(2440..2631)
                     /locus_tag="GO913_RS00020"
                     /old_locus_tag="RCS37_PI0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2696..3019
                     /locus_tag="GO913_RS00025"
                     /old_locus_tag="RCS37_PI0004"
     CDS             2696..3019
                     /locus_tag="GO913_RS00025"
                     /old_locus_tag="RCS37_PI0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_052895110.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNCGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3228..>3659
                     /locus_tag="GO913_RS00710"
                     /pseudo=""
     CDS             3228..>3659
                     /locus_tag="GO913_RS00710"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3697..4215
                     /locus_tag="GO913_RS00035"
                     /old_locus_tag="RCS37_PICDS3697D"
     CDS             3697..4215
                     /locus_tag="GO913_RS00035"
                     /old_locus_tag="RCS37_PICDS3697D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_158538745.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASIEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4222..4455
                     /locus_tag="GO913_RS00040"
     CDS             4222..4455
                     /locus_tag="GO913_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4843..5145
                     /locus_tag="GO913_RS00045"
     CDS             4843..5145
                     /locus_tag="GO913_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158538744.1"
                     /translation="MTHCPVDFISPDFVLRKTIPTIYMAHCPVDFISPDFVLRKTIPTT
                     YMAHCPVDFVSPDFVLRKAIPTIYMAHCSIDLIAHIVSNIHFFYDKAAVNGCYGK"
                     /name="hypothetical protein"
     gene            complement(5173..5529)
                     /locus_tag="GO913_RS00050"
                     /old_locus_tag="RCS37_PI0007"
     CDS             complement(5173..5529)
                     /locus_tag="GO913_RS00050"
                     /old_locus_tag="RCS37_PI0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249939097.1"
                     /translation="MTEGSEENAQMQESQENNSKPPAWRLFYLYLQLTLIYHSIRCIYS
                     VLIGVFMSNELDSLSEVVKGLTKIELPGGGGGETDSPVVEAAIAVGIVGAAAYGIYKLF
                     GRAEPQKDEDKLGF"
                     /name="hypothetical protein"
     gene            complement(5749..6609)
                     /locus_tag="GO913_RS00055"
                     /old_locus_tag="RCS37_PI0008"
     CDS             complement(5749..6609)
                     /locus_tag="GO913_RS00055"
                     /old_locus_tag="RCS37_PI0008"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(6792..7349)
                     /locus_tag="GO913_RS00060"
                     /old_locus_tag="RCS37_PI0009"
     CDS             complement(6792..7349)
                     /locus_tag="GO913_RS00060"
                     /old_locus_tag="RCS37_PI0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            7513..10518
                     /locus_tag="GO913_RS00065"
                     /old_locus_tag="RCS37_PI0010"
     CDS             7513..10518
                     /locus_tag="GO913_RS00065"
                     /old_locus_tag="RCS37_PI0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     ARG             complement(5753..6609)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2268..2355
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(1699..2257)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.078"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3010..4559
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgatctgcc attttctatt cctcactgag cataaacaaa caattgccaa cgaatgttcc
       61 cttgctcggg ggtatgttgg tggtggtttt gtggtgggat tgttttccca cctgcgatag
      121 ttacttcttt accgcaaccg gtacatttat aaatcccttc atattcacat gtttctcccg
      181 gctgatattc agtgtcaaaa accgagttaa acttcttatc tgcttcaaaa atcttgcttt
      241 tgtttcctga acgaacaaga gccatgtaac ctccaaatta ccctctattt tgcacatctg
      301 cactgtgtgt gggtgcattc tacagtatgg ttattggttg cacaagtgca ctgtgctgtt
      361 gtgcatttgt tgtgtttgct gtactatatc ggaggtaaga aggtgatcta aaaaattgcc
      421 aagttgcggc aaaaattgag gtaaaagaaa aatgcagcaa gatcatgaac atgatgattg
      481 ccgcattcag tttcgagacg attggctata ccagttttac aaatatggta aagccaatcg
      541 aaagattcca actgcgatta gtgcttcatt aggtcgcaag ctggatatgc tgaatgctgc
      601 tgataaagtt ggagatttgc ggtctccccc gggaaaccga tttgagaatc tcaaccctcc
      661 attgacaggg tattcatcca tacgggtgaa tgagaagtac cgcttaatat ttaagtgggg
      721 tgaaaacgga atcgaggatt tgtatctcga tccacatgat taccgataga taacaggtcg
      781 tggctgttat cttataaaac aagtaggtga ggcgttccct cacctacact aaaatgtcat
      841 tacaaataat gattaataaa ctaaatgacg tgactatatt taagataatt actcatcgct
      901 acagctggta atagacgaaa tacttatctt cctcatctaa cagatgagaa gtaaaaaatt
      961 taggagtaaa gcatgtccca agcaactaga aagccatcaa ctgtagggga tattattctt
     1021 tatgaatatc tcgaaccaag tggtttaaaa atcaatgact tggctgaaat gttgaatgtg
     1081 catagaaact ctgccagtgc tttagttaac aataatagaa agctgtctac cgatatggct
     1141 tttaggttgg ctaaagcctt cgataccact gcaaagttct ggctaaatat acagcagtct
     1201 gttgatttat gggaggttac tagtagccct cgattgcaag aagaaataga cagagtgatt
     1261 cccgctcatg aaatcataga aaggaaacac attgttgata attctgagaa gaaacatgct
     1321 attgcatgat tttttttaaa aaatataagt tatccctcaa aaagaaggct tctatttata
     1381 gaagccttct ttttgaggga taaaaacact catgttaact ttcttcttac gatcttcttg
     1441 agatccgtgc tctgtacgcg taatctcttg ctctgtaaac gaaaaaaccg cctggggagg
     1501 cggtttttcg aaggttaagt cagttgggga actgcttaac ctggtaactg gcttcagcag
     1561 agcgcagata ccaaatactg tccttccagt gtagccgtag ttaggccacc acttcaagaa
     1621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1681 tggcgttaag gcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1801 cgaactgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1981 tcggtttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct gcggcgtggc
     2041 cttcttcctg ttcctggtct tttgctcaca tgttctttcc ggccttatcc cctgattctg
     2101 tggataactg tgttaccgtt tttgtgtgag ttagtaccgc tcgccgcagc cgaacgaccg
     2161 agcgtagcga gtcagtgagc gaggaagcgg aaaagcgctt ggacgtgcat tttctcctta
     2221 cgcatctgtg cggcatttca cacccggcat ggcgcacttt tcatacaatc cgcactgatg
     2281 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca gggctgcgcc
     2341 ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2401 tcacaggcaa gctgtgaccg tctccgggag ctgcatgtgt cagtggtttt caccgtcatc
     2461 cccgaaacgt gcgaggcagc tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca
     2521 gatatctgcc tgttcatctg cgtccagttc gttgagcttc tccagcagcg ttaatgtctg
     2581 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttagtca ctgatgcctc
     2641 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgcg agaggatgct
     2701 cacgatacgg gttactgatg atgaacatgc tcgtttgctg gaacgttgtg agggtaaaca
     2761 actggcggta tggatgcggc gggtctgcct gggggagccg gttgcccgtt ccgggaggct
     2821 gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcgggaata acctgaatca
     2881 gacagcgagg aaggtgaact gcgggcagtg gtcttccggt gaccgggttc aggtggtggc
     2941 cgcactgatg gccatcgggg acgagctacg ccggctgcgt ctggccgtca gggaacaggg
     3001 gacgcgggat gatagttaag tttcatgccc ggggaaaagg tggtggcagt ggtccggttg
     3061 attacctgct gggaagggag cgtaaccggg aaggggcaac ggtgcttcag ggtaatccgg
     3121 aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc
     3181 tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg
     3241 agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag cattctgtgg gtggagcacc
     3301 aggacaaggg gcggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa
     3361 aacgcctcca gccgtactac gaccgcgcag acaggccgag aattgatgcc tggcagacgc
     3421 tggttaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgctga
     3481 cactccctga taatctgccg gaaacgaaac aggcgcttgc tgagagcgtt acacgggggc
     3541 ttgatgtgct ttatcgtgcc ggagagataa aagaccgtca ggatgtgatt ctggcactca
     3601 cagatgtggg agttgaggtt gtcagggtga cgcgaagcag tatcagcatt gcagatccga
     3661 acggcgggag gaatatcagg ctgaaaggag cattttatga gcaatctttt acagacgggc
     3721 gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac
     3781 gagttcagga ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc
     3841 agagacgcta tagccctgtt caccgcctcg atagaggaat cgctggaaaa acaccgggaa
     3901 ggggtgaaag aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg
     3961 ggcatgatgt tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg
     4021 tatcttggcg ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg
     4081 cagaaactga acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc
     4141 cttgtccttc cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta
     4201 catctgaagg agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag
     4261 cagctgcaac aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg
     4321 cagaagatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag
     4381 ttgagtcagc aggtgcagca cttgagcgag cagacagaac gcttgagcca gttatacagc
     4441 gagaactgga gataagagag gaacggttga tacaggagcg cgaacatgtg ttatccctgg
     4501 aacgggagcg tcagccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     4561 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccatc
     4621 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg
     4681 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgatgaacgt attctggcgg
     4741 tgcgtacaaa acagatgtta tggtaaatat gatttctttt tgcagggttt gccccgcaaa
     4801 ttatctgatc tcatattgcg gaagaccgtt ccaaccgttt acatgactca ctgtcctgta
     4861 gattttattt cccctgattt cgtattgagg aagaccattc caaccatcta catggctcac
     4921 tgtcctgtag attttatttc ccctgatttc gtattgagga agaccattcc aaccacctac
     4981 atggctcact gtcctgtaga ttttgtctcc cctgatttcg tactgaggaa ggccattcca
     5041 accatctaca tggctcactg ttctatagat cttattgctc acattgtctc caatatccat
     5101 ttcttttatg acaaggccgc agttaacgga tgttatggta aatgaatgcc gttttgatgc
     5161 tgccttatgg ttctagaaac cgagtttgtc ctcatctttc tggggttctg cgcgaccaaa
     5221 aagcttataa atcccatatg cagcagcccc tacaatgcca acagctatag ctgcctcaac
     5281 tacagggcta tcggtttcac cgccaccgcc gccaggcagc tctatctttg tgagtccttt
     5341 aactacttct gaaaggctgt ctaattcatt actcataaaa actcctatca agactgagta
     5401 gatacagcgg atgctatggt aaatcagtgt cagctgtaga tataaataaa aaagccgcca
     5461 ggctggcggc ttgctgttat tttcttggct ttcctgcatc tgagcgtttt cctctgaccc
     5521 ttccgtcatt gttacggtca cgaggttgag ttccgccgcc tggtttagga acgacaacgt
     5581 gtgggttgag cggtcgacga tggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5641 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5701 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5761 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5821 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5881 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5941 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6001 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6061 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6121 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6181 cggtcctccg atcgttgtca gaagtaagtt ggcagcagtg ttatcactca tggttatggc
     6241 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6301 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6361 gtcaacacgg gataataccg caccacatag cagaacttta aaagtgctca tcattggaaa
     6421 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6481 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6541 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac gaaaatgttg
     6601 aatactcata ctcttccttt ttcaatatta ttgaagcatt taccagggtt attgtatcat
     6661 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6721 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6781 aaataggcgt atcacgaggc cctttcgtct tcaagaattt tataaaccgt ggagcgggca
     6841 atactgagct gatgagcaat ttccgttgca ccagtgccct tctgatgaag cgtcagcacg
     6901 acgttcctgt ccacggtacg cctgcggcca aatttgattc ctttcagctt tgcttcctgt
     6961 cggccctcat tcgtgcgttc taggatcctc cggcgttcag cctgtgccac agccgacagg
     7021 atggtgacca ccatttgccc catatcaccg tcggtactga tcccgtcatc aatgaaccgg
     7081 actgccacgc cctgagcgtc aaattccttt atcagttgga tcatatcggc agtgtcgcgg
     7141 ccaagacggt cgagcttctt aaccagaatg acatcacctt cctccacctt catcctcagc
     7201 aaatccagcc cttcccggtc tgttgaactg ccggatgcct tatcggtaaa tatacggttt
     7261 gctttcacac ctgcgtcttt gagtgctctg acctgaagat caagagactg ctgactggtt
     7321 gagacccgag cgtaaccaaa aagtcgcata aaaatgtacc ttaaatcgaa tatcggacaa
     7381 ctcatgtcta ttattacaaa tttacgattt aatagacata ttaatgtaac agttttacga
     7441 tgtccgataa tttataacat ttcgtacggt tggaaaaatg ttactaaatg cccgtcaggc
     7501 agggaggccg atatgcccgt tgactttctg accactgagc agactgaaag ctatggcaga
     7561 ttcaccggtg aaccggatga gcttcagctg gcacgatatt ttcaccttga tgaagcagac
     7621 aaggaattta tcggaaaaag cagaggtgat cacaaccgtc tgggcattgc cctgcaaatt
     7681 ggatgtgtcc gttttctggg caccttcctc accgatatga atcatattcc ttccggcgtc
     7741 cggcatttta ccgccagaca gctcgggatt cgtgatatca ccgttcttgc agaatacggt
     7801 cagagggaaa atacccgccg tgagcatgca gcgctgatac gtcagcacta tcagtatcgt
     7861 gaatttgcct ggccctggac atttcgcctt acccgtcttt tatatacccg gagctggata
     7921 agcaacgaac gtcctggcct gcttttcgat ctggcgacag ggtggcttat gcaacatcgt
     7981 attattctcc ccggagccac tacgctgacc cggttgattt cagaggtaag ggaaaaggcg
     8041 acgttgcgcc tgtggaacaa actggcactg ataccgtcag ccgaacagcg ttcacagctg
     8101 gagatgctgc tggggccaac tgattgcagc cgcctgtctt tactggaatc actgaaaaag
     8161 ggccctgtga ccatcagtgg tccggcgttt aatgaagcaa ttgaacgctg gaaaactctg
     8221 aacgattttg gcctgcatgc tgaaaacctg agtacactcc cggctgtgcg cctgaaaaat
     8281 ctcgcacgtt atgctggtat gacttcggtg ttcaatattg ccaggatgtc accgcagaaa
     8341 aggatggcgg ttctggttgc ctttgtcctt gcatgggaaa cgctggcgct ggatgatgca
     8401 ttggacgttc tggacgccat gctggccgtt atcatccgtg acgccagaaa gattgggcag
     8461 aaaaaacggc tccgctcgct gaaggatctg gataaatctg cattggcgct cgccagcgca
     8521 tgttcgtacc tgctgaaaga agaaacaccg gacgaatcga ttcgtgctga ggtgttcagc
     8581 tacatcccaa ggcaaaagct ggctgaaatc atcacgcttg tccgtgaaat tgcccggccc
     8641 tcagacgata attttcatga agaaatggtg gagcagtacg ggcgcgttcg tcgtttcctg
     8701 ccccatctgc tgaataccgt taaattttca tccgcacctg ccggggttac cactctgaat
     8761 gcctgtgact acctcagccg ggagttcagc tcacggcggc agttttttga cgacgcacca
     8821 acggaaatta tcagtcggtc atggaaacgg ctggtgatta acaaggaaaa acatatcacc
     8881 cgcaggggat acacgctctg ctttctcagt aaactgcagg atagtctgag gcggagggat
     8941 gtctacgtta ccggcagtaa ccggtgggga gatcctcgtg caagattact acagggtgct
     9001 gactggcagg caaaccggat taaggtttat cgttctttgg ggcacccgac agacccgcag
     9061 gaagcaataa aatctctggg tcatcagctt gatagtcgtt acagacaggt tgctgcacgt
     9121 ctttgcgaaa atgaggctgt cgaactcgat gtttctggcc cgaagccccg gttgacaatt
     9181 tctcccctcg ccagtcttga tgagccggac agtctgaaac gactgagcaa aatgatcagt
     9241 gatctactcc ctccggtgga tttaacggag ttgctgctcg aaattaacgc ccataccgga
     9301 tttgctgatg agtttttcca tgctagtgaa gccagtgcca gagttgatga tctgcccgtc
     9361 agcatcagcg ccgtgctgat ggctgaagcc tgcaatatcg gtctggaacc actgatcaga
     9421 tcaaatgttc ctgcactgac ccgacaccgg ctgaactgga caaaagcgaa ctatctgcgg
     9481 gctgaaacta tcaccagcgc taatgccaga ctggttgatt ttcaggcaac gctgccactg
     9541 gcacagatat ggggtggagg agaagtggca tctgcagatg gaatgcgctt tgttacgcca
     9601 gtcagaacaa tcaatgccgg accgaaccgc aaatactttg gtaataacag agggatcacc
     9661 tggtacaact ttgtgtccga tcagtattcc ggctttcatg gcatcgttat accggggacg
     9721 ctgagggact ctatctttgt gctggaaggt cttctggaac aggagaccgg gctgaatcca
     9781 accgaaatta tgaccgatac agcaggtgcc agcgaacttg tctttggcct tttctggctg
     9841 ctgggatacc agttttctcc acgcctggct gatgccggtg cttcggtttt ctggcgaatg
     9901 gaccatgatg ccgactatgg cgtgctgaat gatattgcca gagggcaatc agatccccga
     9961 aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa
    10021 gtacaggttt cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact
    10081 caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat
    10141 gatgaagatt accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc
    10201 gttgccagag ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt
    10261 caggaagatc aactgggcac actggggctg gtcactaacg ccgtcgtgtt atggaacact
    10321 atttatatgc aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa
    10381 gatatcgcac gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc
    10441 ttcacgctgg cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag
    10501 gcagaaaacg ttgcttaacg tgagttttcg ttccactgag cgtcagaccc c
//
