LOCUS       NZ_LT598667            22080 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Enterococcus faecium isolate Ef_aus00233 plasmid 5.
ACCESSION   NZ_LT598667
VERSION     NZ_LT598667.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4067785
            Assembly: GCF_900092475.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Lee,Jean., Stinear,Tim., Seemann,Torsten., Kwong,Jason.,
            Buultjens,Andrew., Goncalves da Sivla,Anders., Baines,Sarah.,
            Carter,Glen., Bulach M,Dieter. and Monk,Ian.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2016) UNIVERSITY OF MELBOURNE, Doherty Centre for
            Applied Microbial Genomics, Doherty Institute for Infection and
            Immunity, 792 Elizabeth St, Melbourne, VIC, 3000, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 02:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,254
            CDSs (total)                      :: 3,162
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 36 of 120
            Pseudo Genes (incomplete)         :: 81 of 120
            Pseudo Genes (internal stop)      :: 26 of 120
            Pseudo Genes (multiple problems)  :: 19 of 120
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT598667.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..22080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="Ef_aus00233"
                     /isolation_source="Clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="5"
                     /geo_loc_name="Australia"
                     /collection_date="2011"
     gene            1..>504
                     /locus_tag="BN9748_RS16725"
                     /pseudo=""
     CDS             1..>504
                     /locus_tag="BN9748_RS16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            579..1259
                     /locus_tag="BN9748_RS16730"
     CDS             579..1259
                     /locus_tag="BN9748_RS16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            1457..2062
                     /locus_tag="BN9748_RS16735"
     CDS             1457..2062
                     /locus_tag="BN9748_RS16735"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            2078..2650
                     /locus_tag="BN9748_RS16740"
     CDS             2078..2650
                     /locus_tag="BN9748_RS16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(3060..4019)
                     /locus_tag="BN9748_RS16745"
     CDS             complement(3060..4019)
                     /locus_tag="BN9748_RS16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="WP_000222577.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINFL
                     KEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPISCS
                     VRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEFGHI
                     EGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFKSITF
                     DCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQVDQAF
                     ISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
                     /name="IS30-like element IS1252 family transposase"
     gene            complement(4147..4827)
                     /locus_tag="BN9748_RS16750"
     CDS             complement(4147..4827)
                     /locus_tag="BN9748_RS16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(5055..5330)
                     /gene="prgO"
                     /locus_tag="BN9748_RS16755"
     CDS             complement(5055..5330)
                     /gene="prgO"
                     /locus_tag="BN9748_RS16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(5302..6255)
                     /locus_tag="BN9748_RS16760"
     CDS             complement(5302..6255)
                     /locus_tag="BN9748_RS16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            6867..8360
                     /locus_tag="BN9748_RS16765"
     CDS             6867..8360
                     /locus_tag="BN9748_RS16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            8495..8791
                     /locus_tag="BN9748_RS16770"
     CDS             8495..8791
                     /locus_tag="BN9748_RS16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            8892..9743
                     /locus_tag="BN9748_RS16775"
     CDS             8892..9743
                     /locus_tag="BN9748_RS16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            9764..9976
                     /locus_tag="BN9748_RS16780"
     CDS             9764..9976
                     /locus_tag="BN9748_RS16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            10409..11089
                     /locus_tag="BN9748_RS16785"
     CDS             10409..11089
                     /locus_tag="BN9748_RS16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(11197..11877)
                     /locus_tag="BN9748_RS16790"
     CDS             complement(11197..11877)
                     /locus_tag="BN9748_RS16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_002323195.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCHT
                     TIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDIWL
                     RKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVKYLN
                     NLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLM
                     GIPA"
                     /name="IS6 family transposase"
     gene            complement(11926..12516)
                     /locus_tag="BN9748_RS16795"
     CDS             complement(11926..12516)
                     /locus_tag="BN9748_RS16795"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_002323194.1"
                     /translation="MVGKFLTTISCACFGGALAVIGSSAYNKIHERPSAANERLEIGNW
                     EADTVAGKTGRARIVTLVDRRSRFSLLGKVEKKISKNVVDCMINLLRNVGEDKCKTVTP
                     DRGKEFSQHSRITLELNGTKVYFPDPHAPWQRGTNENTNGLLREYSPKGEDISGIFDSQ
                     IQEWANKLNTRPRKCLNWKTPYEVFLVLLQSSP"
                     /name="IS30 family transposase"
     gene            <12437..12817
                     /locus_tag="BN9748_RS17620"
                     /pseudo=""
     CDS             <12437..12817
                     /locus_tag="BN9748_RS17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /name="toxin zeta"
     gene            complement(13080..>13820)
                     /gene="erm(B)"
                     /locus_tag="BN9748_RS16805"
                     /pseudo=""
     ARG             complement(13084..13817)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(13942..14025)
                     /locus_tag="BN9748_RS16810"
     CDS             complement(13942..14025)
                     /locus_tag="BN9748_RS16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="WP_031929417.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuator leader peptide
                     ErmL"
     gene            complement(14074..>14153)
                     /locus_tag="BN9748_RS17785"
                     /pseudo=""
     CDS             complement(14074..>14153)
                     /locus_tag="BN9748_RS17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /name="peptide-binding protein"
     gene            complement(14828..15508)
                     /locus_tag="BN9748_RS16820"
     CDS             complement(14828..15508)
                     /locus_tag="BN9748_RS16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(15655..15810)
                     /locus_tag="BN9748_RS16825"
     CDS             complement(15655..15810)
                     /locus_tag="BN9748_RS16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            16427..16648
                     /locus_tag="BN9748_RS16830"
     CDS             16427..16648
                     /locus_tag="BN9748_RS16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            16649..16906
                     /locus_tag="BN9748_RS16835"
     CDS             16649..16906
                     /locus_tag="BN9748_RS16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002377879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(17042..17644)
                     /locus_tag="BN9748_RS16840"
     CDS             complement(17042..17644)
                     /locus_tag="BN9748_RS16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_010729575.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(17701..18381)
                     /locus_tag="BN9748_RS16845"
     CDS             complement(17701..18381)
                     /locus_tag="BN9748_RS16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <18458..18763
                     /locus_tag="BN9748_RS16850"
                     /pseudo=""
     CDS             <18458..18763
                     /locus_tag="BN9748_RS16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            18859..19539
                     /locus_tag="BN9748_RS16855"
     CDS             18859..19539
                     /locus_tag="BN9748_RS16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <19593..19817
                     /locus_tag="BN9748_RS16860"
                     /pseudo=""
     CDS             <19593..19817
                     /locus_tag="BN9748_RS16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(19953..20367)
                     /locus_tag="BN9748_RS16865"
                     /pseudo=""
     CDS             complement(19953..20367)
                     /locus_tag="BN9748_RS16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(20370..20654)
                     /locus_tag="BN9748_RS16870"
     CDS             complement(20370..20654)
                     /locus_tag="BN9748_RS16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     ARG             complement(13081..13817)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     Typing          16809..16984
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          2..504
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6868..8360
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17115..17644)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          19595..19817
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.97e-113"
ORIGIN
        1 atggatgaaa aaacggaatt aacagtcaaa tatcaaaatg aattgaattt agtaccgctg
       61 aaaaatttca atgctaagca gatggattta ttttttgcat tatgcgctcg tatgaaagat
      121 cgtggtttga aaaaagttag attttctttc gaggaattaa aagagttaag tgattataag
      181 atgactgcta caaaagcctt tgtcggtgat ttagaaaagc tctataaaga tatgcttaat
      241 cttacttacc gtactgaaga tgaggattct attcgctact ttgttttgtt tactggtttt
      301 gaaatcaata aaacaaaaaa gtttgttgaa gtcagtgtta atccggacct agaatatatt
      361 attaatggtt taacagctga atttagtaga tttgaattat ctgctttcac gtcgttgcgt
      421 tctacgtatg caaaaactct attccgactg cttatgcagt accgttctac tggttattat
      481 gtagttcgta ttgaagagtt taggggttct gttgcaaagt tttaaatcta ctatcaaata
      541 aggtagaata atagaaaaag atagcaggag gaatgacgat gaatcatttt aaaggaaagc
      601 aatttcagca ggatgtgatt attgtagccg tgggctacta tcttcgttat aaccttagct
      661 atcgtgaagt tcaagaaatc ttatatgatc gtggcattaa cgtttctcat acgacgattt
      721 atcgttgggt gcaagaatat ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa
      781 aatcctttta ttcatggaaa atggatgaaa cgtacatcaa aattaaagga aaatggcatt
      841 atttgtatcg agccatcgat gcagatggtt taaccttgga tatttggtta cgtaaaaaac
      901 gggacacaca agcagcctat gcttttctta agcggttagt gaagcagttt gatgaaccga
      961 aggttgtagt cacagataaa gccccctcta ttacaagtgc ctttaagaaa ctaaaagaat
     1021 acggctttta tcaagggaca gaacatcgta ccattaaata cctgaataat ttgattgaac
     1081 aagaccatcg tccagtaaag agacgcaata aattctatcg aagtttacgc actgcctcta
     1141 ccacgattaa aggcatggaa gccattcgag gattatataa gaaaacccga aaagaaggca
     1201 ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc ccagcttaaa
     1261 tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga accaactaga
     1321 ctgtgcaaaa tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga
     1381 cacataaaca aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa
     1441 gttaaggaga tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt
     1501 ggctaaggtt gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa
     1561 aataaatgaa atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt
     1621 ttcggatatt tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt
     1681 tcgatttgca ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca
     1741 aacttctttt gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg
     1801 agaaggaaat ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca
     1861 aaaagttgta gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag
     1921 tcccataaac gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga
     1981 cgatcgcgag ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga
     2041 gtatacgata gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg
     2101 tgtctcaacc gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt
     2161 agagaagaag aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt
     2221 caatcagctg ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg
     2281 atttgctcgt aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt
     2341 aacgatcaag gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac
     2401 acggacgttg ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga
     2461 aggaaaaatg tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac
     2521 aatcacaccg aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga
     2581 agttgaagct ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga
     2641 agaaagttaa aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat
     2701 atgagtctat tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa
     2761 aaaatgtgta tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact
     2821 atattaggat accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat
     2881 atcgttcagc gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata
     2941 atctattgac tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct
     3001 ttcatttaag aaccaaataa ctatcggttc tgttgcaaag ttttaaataa tttgatttgt
     3061 caaattaagc tagacaaaat atcttcattc atgtaactca taaatacttc caggggtgtt
     3121 tgataatcca aggactttcg tggaatattg tttctcttat tcgccactga agagataaaa
     3181 gcttggtcga cttgattaaa atccatgttt tttggcagac catcttttcg cagcaagcca
     3241 ttggaatttt cattcagtgc tcgttgcgaa ggagtccctg gatcagcgaa ataaatagcg
     3301 atgtcatgtt ggttacttaa agccttccaa ttagagaatt ctttcccaca atcaaaggta
     3361 atggatttaa ataaatgtct gggaatcgat ccaaaccagt ggttcagagt attctcaata
     3421 tcgatcgcct tgcgcccctc gggcttcaag gtgatgatga ttttcgatag acgttctacc
     3481 aaagttatga cagcactctt atgatgaatg cctacaatgg tatcgccttc gatatggccg
     3541 aactcttctt taaatgtcgg gtaatcagtt tctctttcaa cgatattccg tttaaaagcc
     3601 tgttttcctc ggcgttcttg atgtccattt ggtttccgct tacccttcat gggcaaagtc
     3661 gattcatcga acaccttttc cttgaactgg cgataaaggg ttcttacgga acagctgatt
     3721 ggtttctctt tacgcccaat gataacgtct ggcgtccagc cttgggcaat tttttcttta
     3781 atgtaggact gttgtttctt tggcaagacg atgtttcgtc tgccacaccg tttcttattt
     3841 tctttatact gttggtagta ttcgagggcc gtatggcctt ctttcaagaa attaatgacg
     3901 ttataaatcg gtgttcgaga tcgtttcagg tactcagcaa ttttcacaat tttgatgttt
     3961 tggtggtagt aagactctat catcaccagt tcatccgtgg taagatgggt gtaggtcatt
     4021 cgtgatcact cctatgtttt ctttagtcgg aattacattt tgagtgtagc acgaatggct
     4081 tatttatttg tctagcttaa ttttacaatc ggcgtaaaga ataaaatccc ttacggtatc
     4141 tatgatttaa gctgggattc ccaataatac cttgatttca gtacagaccg aaaacccgaa
     4201 gagagtgcct tcttttcggg ttttcttata taatcctcga atggcttcca tgcctttaat
     4261 cgtggtagag gcagtgcgta aacttcgata gaatttattg cgtctcttta ctggacgatg
     4321 gtcttgttca atcaaattat tcaggtattt aatggtacga tgttctgtcc cttgataaaa
     4381 gccgtattct tttagtttct taaaggcact tgtaatagag ggggctttat ctgtgactac
     4441 aaccttcggt tcatcaaact gcttcactaa ccgcttaaga aaagcatagg ctgcttgtgt
     4501 gtcccgtttt ttacgtaacc aaatatccaa ggttaaacca tctgcatcga tggctcgata
     4561 caaataatgc cattttcctt taattttgat gtacgtttca tccattttcc atgaataaaa
     4621 ggatttttta tttttctttt tccaaatttg atagagtagt ttgccatatt cttgcaccca
     4681 acgataaatc gtcgtatgag aaacgttaat gccacgatca tataagattt cttgaacttc
     4741 acgatagcta aggttataac gaagatagta gcccacggct acaataatca catcctgctg
     4801 aaattgcttt cctttaaaat gattcatcgt cattcctcct gctatctttt tctattattc
     4861 taccttattt gatagtagat ttaaaacttt gcaacagaac catatttagt atagtaaaaa
     4921 ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat attttgaagg
     4981 attaggatta ataaagctaa aaatatagta agtatactaa atatatttac tatatctagt
     5041 atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc ctcgtcgttt
     5101 aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg aatatctttt
     5161 gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac tggttttctt
     5221 tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt aattgaaata
     5281 tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat ttagtttaat
     5341 ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag gtgtataatc
     5401 aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta attctgtaaa
     5461 gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt ggataccatt
     5521 ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa tgtttgattc
     5581 accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa
     5641 aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg tgacaatcgg
     5701 aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt ttaattcttg
     5761 cattgcaata atataatctg cagcagttaa gaatgcacga accacattat ctgttgatgg
     5821 tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa
     5881 ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat taagttcttc
     5941 agatccagga gagccagcca taatatataa atgtggactt acttgagtaa tacctgaaac
     6001 ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa tttgatacgt
     6061 tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca acacgtcttt
     6121 ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct taccaatgcc
     6181 gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt taaatgcagt
     6241 ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag tatacttcat
     6301 atacttaata tattcgatat atctaatata ctccaaattg ataagaattc agactttata
     6361 aggaatatag tggatatact aaatatattt gctatattca gtatatcaga tatattcaaa
     6421 atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga ggtaaactta
     6481 attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag tggtactctt
     6541 taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc agaatcaatt
     6601 atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc
     6661 taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt tattctacca
     6721 aaaaaaggcg agcaactcaa cagaagaaag tttatttagt gtacttaaat taaatattct
     6781 taaacactga agagagaaca catactatgg ctgagattaa agctaaactg tgttctcttt
     6841 ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct attcttaaaa
     6901 acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt catattgatt
     6961 ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat gttgggcgtg
     7021 gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta acgcattgga
     7081 cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg gttaaaggtc
     7141 attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat tcaaaagaga
     7201 accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg ccaacgctaa
     7261 ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca ggctatgtca
     7321 caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc
     7381 tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc ggaattgctc
     7441 gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat tcttttgctg
     7501 actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac
     7561 gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg
     7621 atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt cgaaataatg
     7681 tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat acttgcgaat
     7741 acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca gaattagtaa
     7801 agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa ttcattattg
     7861 aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc attcaacgac
     7921 gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat aaaaatgaat
     7981 ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag ccttatttga
     8041 acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca cttgataaga
     8101 tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga ggaagaaatg
     8161 gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag
     8221 aaaagagact agattattca acaaggatca cagaggtatt tggactcaac gagagcttag
     8281 tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt
     8341 ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccctatacaa tatatctatg
     8401 atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata cttataactg
     8461 cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc
     8521 acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag tgttctgtga
     8581 gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa aaacagttgc
     8641 ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga ctatggatca
     8701 ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa tagctaacga
     8761 gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa aaattggttc
     8821 attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa gaatttaggg
     8881 aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct ctgaaaagaa
     8941 atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa ctttgtagtt
     9001 acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt ggaacggctt
     9061 attacttgat aaatgatccc aataggattg cagcatcaga catttttaca gtgctaatga
     9121 cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa tttaccaggc
     9181 aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt gtaaatgcaa
     9241 ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa ggagtgaagg
     9301 attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt gaagtgggta
     9361 ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca attgaaaaag
     9421 aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt gattttggaa
     9481 gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa tctactgtta
     9541 agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat cgaagaaaag
     9601 agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga aaattgtggt
     9661 tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt agtgatttag
     9721 aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc ctctatacag
     9781 gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt tattcaaagt
     9841 gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca ttgaataccg
     9901 aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa agcaacaaga
     9961 gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga acaattcaag
    10021 gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat aaggaaacgt
    10081 gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt ctcaaaaata
    10141 aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg atagtaaaag
    10201 gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt aaattggagg
    10261 aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag aaattgatcg
    10321 aagatgtgac gaacggttct gttgcaaagt tttaaatcta ctatcaaata aggtagaata
    10381 atagaaaaag atagcaggag gaatgacgat gaatcatttt aaaggaaagc aatttcagca
    10441 ggatgtgatt attgtagccg tgggctacta tcttcgttat aaccttagct atcgtgaagt
    10501 tcaagaaatc ttatatgatc gtggcattaa cgtttctcat acgacgattt atcgttgggt
    10561 gcaagaatat ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa aatcctttta
    10621 ttcatggaaa atggatgaaa cgtacatcaa aattaaagga aaatggcatt atttgtatcg
    10681 agccatcgat gcagatggtt taaccttgga tatttggtta cgtaaaaaac gggacacaca
    10741 agcagcctat gcttttctta agcggttagt gaagcagttt gatgaaccga aggttgtagt
    10801 cacagataaa gccccctcta ttacaagtgc ctttaagaaa ctaaaagaat acggctttta
    10861 tcaagggaca gaacatcgta ccattaaata cctgaataat ttgattgaac aagaccatcg
    10921 tccagtaaag agacgcaata aattctatcg aagtttacgc actgcctcta ccacgattaa
    10981 aggcatggaa gccattcgag gattatataa gaaaacccga aaagaaggca ctctcttcgg
    11041 gttttcggtc tgtactgaaa tcaaggtatt attgggaatc ccagcttaaa tcatagatac
    11101 cgtaagggat tttattcttt atttaaaact ttgcaacaga accttctgtt gcaaagttcc
    11161 ccaaaagagt atagaagtcc tagtttaata ttttgactat gctgggatcc ccatcagaac
    11221 tttaatttca gtcgacaccg aaaatccgaa gagcgttccg tttcttcggt tctttttgta
    11281 tattcctcga atagtctcca tgcccttaat cgtggttgag gcagttcgta aacttcgata
    11341 aaatttatta cggcgcttaa ttggacgatg gtcctgctca attaaattat tgagatactt
    11401 aactgtccga tgttctgtct tggtatataa accgttaatc tgtagctttc tgaatgcaga
    11461 gctaatcgat ggtgctttat cggttacaat cactctaggt tgtccaaatt gtttatggag
    11521 ccgttttaag aaagcataag ccgcctgagt atcccgtttc tttcgtaacc aaatgtctaa
    11581 agttaatcca tcagaatcaa tcgcacgata gagataatgc caatggcctt ttattttgat
    11641 ataggtctcg tccattttcc acgagtagaa ggactgtcta tttttcttct tccaaagatg
    11701 atagaggatt ttactgtact cttgaaccca acgataaatc gtagtgtgac aaacatttat
    11761 tccgcgatca tacagtaatt cctgaacttc acgatagctc agattgtaac gcaggtagta
    11821 accaacagcg acaataatca catctttttg aaattgtttc cctttgaaat gattcatctg
    11881 tctaccctca ctctcgtttt cttacatttt acaataaatc aggagttatg gggaactttg
    11941 caacagaacc aaaaatactt cataaggggt tttccaattt agacatttcc gtggtcgcgt
    12001 gtttaattta ttcgcccatt cttgaatttg actatcgaaa atacctgaaa tatcttcacc
    12061 tttgggtgaa tattctcgta ataaaccatt agtattttcg tttgtaccac gttgccatgg
    12121 agcatgtgga tctggaaaat acactttagt gccatttaat tctagcgtta ttcgactatg
    12181 ttgggagaat tcttttcctc tatcgggtgt aacggtttta catttatctt ctcctacatt
    12241 tcttagtaga ttgatcatac aatcaaccac atttttagag attttctttt caaccttacc
    12301 aagtagggag aaacgagatc tacgatcgac tagagttaca atacgagccc tacctgtttt
    12361 accggctaca gtatcagctt cccaattacc aatctcaagg cgctcattgg cagcagatgg
    12421 cctttcatgg atcttattgt atgcagatga tccaatgaca gctagggcac caccaaaaca
    12481 agcgcatgat attgttgtca aaaacttacc gaccaattta gaaacccttc ataaaacggg
    12541 cttatttagc gatataaggc tttacaatag agaaggagtg aaactctatt caagcttaga
    12601 aacgccttcc attagtccga aagagacctt agaaagagaa ttaaatcgta aagtatcagg
    12661 gaaagaaatt caaccgactt tggagagaat agagcaaaaa atggttcaaa atcaacacca
    12721 agaaacccct gaatttaaag caattcaaca aaaattggaa agcttgcagc cacctacacc
    12781 accaataccc aaaacaccta aacttccagg tatttaaacc ttaaaaaagg aaaagagtag
    12841 ttaccaaaaa acggtaacta ctcttttttt ataaaaacat ttcctcatgt tggggtcccg
    12901 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgtttttttc
    12961 taatttcatt ttgaggtgaa ttaattggta gttattagag gtgccctata cctaataatt
    13021 tatctacatt ccctttagta acgtgtaact ttccaaattt acaaaagcga ctcatagaat
    13081 tatttcctcc cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta
    13141 acggtactta aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta
    13201 aacagttgac gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa
    13261 tatttatctg gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt
    13321 ggtttaggat gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg
    13381 tgcaagagca accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca
    13441 acaatcagat agatgtcaga cgcatggctt tcaaaaacca cttttttaat aatttgtgtg
    13501 cttaaatggt aaggaatact cccaacaatt ttatacctct gtttgttagg gaattgaaac
    13561 tgtagaatat cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat
    13621 aagttgaata gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt
    13681 ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa
    13741 ttcaattgtt ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat
    13801 tttatatttt tgttcatgta atcactcctg aagtgattac atctataaac aaatacagaa
    13861 gttaaacgat ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg
    13921 gtaggaatta atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgtttcaa
    13981 aacagtagat gttttatcta cataacgcat ttggaatacc aacatgacga atccctcctt
    14041 cttaattaca aatttttagc atctaattta acttcaattc ctattataca aaatttttaa
    14101 tgcactatca acacactctt aagtttgctt ctaagtctta tttccataac tttagggtta
    14161 accatacgca agaccaatca ctctcggaca atactcatga ttttaatgat aaaatccatg
    14221 ttaaacccat agataagaaa tacacctgca ataaccgtta cctgtttgtg ccaatttaaa
    14281 aatgcaccac ttttttataa ttaaaacggt tgaaaactgt accactaata actcacaata
    14341 gagagatgtc accgtcaagt taaatgtaca aaataacagc gaaattttta aatctatttc
    14401 ttatcgatac aaattcctcg taggcgctcg ggaccccatg ttttaaaagc tgatcgagaa
    14461 ataaaacaca caacggtatt ctttcttttt tcagttggta cattttgcag tctgtaacgt
    14521 gtcatttact gtttgttacc ttaacatatc cagtcgaaaa cacaagggta tataaacgag
    14581 caagctcgcc cttgtgtttt ctttgtgtct atgttgcgta ctcgtcacag caaacgacac
    14641 gaacacactg gcgaaaatga accaacaaaa cgaaaaaaga aaggagcgta cactgtaatt
    14701 tatttctagc ttttaaaaca tgaggaaaac agcttcactt cctcaaacgc ctaacaaaaa
    14761 tggttgtcaa aaaggttctg ttgcaaagtt ttaaataaag aataaaatcc cttacggtat
    14821 ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc gaaaacccga
    14881 agagagtgcc ttcttttcgg gttttcttat ataatcctcg aatggcttcc atgcctttaa
    14941 tcgtggtaga ggcagtgcgt aaacttcgat agaatttatt gcgtctcttt actggacgat
    15001 ggtcttgttc aatcaaatta ttcaggtatt taatggtacg atgttctgtc ccttgataaa
    15061 agccgtattc ttttagtttc ttaaaggcac ttgtaataga gggggcttta tctgtgacta
    15121 caaccttcgg ttcatcaaac tgcttcacta accgcttaag aaaagcatag gctgcttgtg
    15181 tgtcccgttt tttacgtaac caaatatcca aggttaaacc atctgcatcg atggctcgat
    15241 acaaataatg ccattttcct ttaattttga tgtacgtttc atccattttc catgaataaa
    15301 aggatttttt atttttcttt ttccaaattt gatagagtag tttgccatat tcttgcaccc
    15361 aacgataaat cgtcgtatga gaaacgttaa tgccacgatc atataagatt tcttgaactt
    15421 cacgatagct aaggttataa cgaagatagt agcccacggc tacaataatc acatcctgct
    15481 gaaattgctt tcctttaaaa tgattcatcg tcattcctcc tgctatcttt ttctattatt
    15541 ctaccttatt tgatagtaga tttaaaactt tgcaacagaa cccgtcatgc tataatcacc
    15601 ttaattgaac aacagtctaa cagcaaaagc agtgcgccaa cactaatgct tttgctattt
    15661 tttttgctca ttttcctttc cgttagcatt aacccaaatt gtaataagac cagacttact
    15721 aagacccatt tgtttcgcaa ttttatcaag attatctgcc acttccttag taagcgttac
    15781 aacgactctc tttttttcat tactgatcat aattcgtatc ctccaacgac tttttcttaa
    15841 aaaccatagt aacaaaaaag taactaaggt gcaacagttt agatactaat tagcgaataa
    15901 taggccttaa acccttggca ttactgattt tgtcaaaaaa aaacgctgaa acccttggga
    15961 gagtaaggct aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc
    16021 cccccctcct ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc
    16081 cccgaacccc gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc
    16141 agctatcggc ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca
    16201 ttctcgcttc tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa
    16261 atcgcttaaa acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc
    16321 gataatataa aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc
    16381 gacgtactca cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa
    16441 atcagaaaaa ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga
    16501 ttacaaccaa gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata
    16561 tcgctctgcc ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa
    16621 agtcataacc accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca
    16681 actagaaaaa ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt
    16741 cgaagaaacc accgacctct ttgacctact tctcacaaat attctggaat taaactctga
    16801 agaacaaaaa gagcataagg gaaaactgat agcaccttcc tacaggaagg cggctaagtc
    16861 ggctgtgcca actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat
    16921 ccattttgga cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg
    16981 tagttttggt catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct
    17041 cttatccttt atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt
    17101 gcccgataat ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg
    17161 acattgcata gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca
    17221 tcatctctct aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt
    17281 ctgcaccacc atgagcgata atatcacttt tagcatattg aaatttttct ggattatcca
    17341 tatgagcaaa atatcgcacc ataccttttg cattagcaca tttttgcggg tttggagacc
    17401 gaatcttttg agttatctca cgtatttgat catacgtctt tttaccgcta aacattatca
    17461 aaacatgcca atgaggcttt ttcaattcac cgtcaggatt cacatcttta tcatgaagtg
    17521 gactctcaac ccaaggaaca tgtaactcat ctaaaaactc cctccaattt tcaggagccg
    17581 attcaggata gactacaaac gtccaattcc ttgtgcgttc atctttagtt tttctactcg
    17641 tcatgcggtt ctgttgcaaa gttttaaata aagaataaaa tcccttacgg tatctatgat
    17701 ttaagctggg attcccaata ataccttgat ttcagtacag accgaaaacc cgaagagagt
    17761 gccttctttt cgggttttct tatataatcc tcgaatggct tccatgcctt taatcgtggt
    17821 agaggcagtg cgtaaacttc gatagaattt attgcgtctc tttactggac gatggtcttg
    17881 ttcaatcaaa ttattcaggt atttaatggt acgatgttct gtcccttgat aaaagccgta
    17941 ttcttttagt ttcttaaagg cacttgtaat agagggggct ttatctgtga ctacaacctt
    18001 cggttcatca aactgcttca ctaaccgctt aagaaaagca taggctgctt gtgtgtcccg
    18061 ttttttacgt aaccaaatat ccaaggttaa accatctgca tcgatggctc gatacaaata
    18121 atgccatttt cctttaattt tgatgtacgt ttcatccatt ttccatgaat aaaaggattt
    18181 tttatttttc tttttccaaa tttgatagag tagtttgcca tattcttgca cccaacgata
    18241 aatcgtcgta tgagaaacgt taatgccacg atcatataag atttcttgaa cttcacgata
    18301 gctaaggtta taacgaagat agtagcccac ggctacaata atcacatcct gctgaaattg
    18361 ctttccttta aaatgattca tcgtcattcc tcctgctatc tttttctatt attctacctt
    18421 atttgatagt agatttaaaa ctttgcaaca gaaccgggta actgaccctg cattaagaga
    18481 cttgctaaca aatttagtca tagaagtgtt tagctatgtt gctgaaaacg aacggaaaaa
    18541 aatcaaagag cgtcaaaaac aaggcattga aattgcaaag aaaaatggtc gttatactgg
    18601 taaaaagatt caataccatg ccaatgcaac aggaaaaaac aaattgattt acgatgaaat
    18661 tgttgatggt ctgaagaaaa aagaaactat ttctaaaata gcaaagaacg caggagttac
    18721 aagaaaaacc gtttatcgag taaaaagaga tttaggaatg taatatgttc cagttagaaa
    18781 gaagggttct gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag
    18841 atagcaggag gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt
    18901 attgtagccg tgggctacta tcttcgttat aaccttagct atcgtgaagt tcaagaaatc
    18961 ttatatgatc gtggcattaa cgtttctcat acgacgattt atcgttgggt gcaagaatat
    19021 ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa
    19081 atggatgaaa cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat
    19141 gcagatggtt taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat
    19201 gcttttctta agcggttagt gaagcagttt gatgaaccga aggttgtagt cacagataaa
    19261 gccccctcta ttacaagtgc ctttaagaaa ctaaaagaat acggctttta tcaagggaca
    19321 gaacatcgta ccattaaata cctgaataat ttgattgaac aagaccatcg tccagtaaag
    19381 agacgcaata aattctatcg aagtttacgc actgcctcta ccacgattaa aggcatggaa
    19441 gccattcgag gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc
    19501 tgtactgaaa tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat
    19561 tttattcttt atttaaaact ttgcaacaga accagtttag gagcttaatg gatatcccag
    19621 aatactatca aatgggtaac attgatcaaa aagtattaaa tcctgcatta cgagaactaa
    19681 aaaaatactt caatgatctt aaagtcacaa aaataaaagc aaaaaagggc aataaaatcg
    19741 ccaaattaga atttactttt tcaagcctaa cgtcagattt gccaaagatt tctctgcgta
    19801 actggttaga agaataaata aaaaagaact cgccccctta cattaagcaa gtagacaagt
    19861 cccttacaac cgactaagat tgctagtaca ttctagcata ttgtatgggg cttctaaata
    19921 aaaaacagat aggaataact cctatctgtt ttttatcgtt ttagaaaatt tgttgggtcg
    19981 aaatttctag aagtgactgt ttgactggtt tctttttgat gattctttgg tcgatttcga
    20041 tttgatggat ttagtttagc ttcgattttt tctttcactt cttgatatac tttagaattt
    20101 gtgccaataa gaacgcctgt atcttttaga tataaatcag caaattctgc gatgatttca
    20161 gtcatgatga aatttctttt cccatagcat ttagttttgc ctgatgctgg tagtggtcgt
    20221 gttggtatcg ggttcataac tcatacataa aaagcgaatg gcttccgagt agttattgat
    20281 atctggattt ttttcgatga tggaatctaa aacgtcaaca tgactagggt acaaagtaac
    20341 atgtttttgg acagaagttt ttctcatggt tatatcattc ctccgataag catttcaatg
    20401 atttgattat ttgtgtagac atactcgctt aatatttgag tattttctgt ttggatagca
    20461 ataaatttat cgagcaataa atcgaattgg tcttttttgt aagtcgtaag ttgacggtca
    20521 aaatcacttt cgattaaaac ttttagatat ttatttcgac tccatttttc tcctgtttta
    20581 ttcgtgagtt cttggcaacg tttgtctatt gttgcaacaa actttggatc aatattccga
    20641 actaaaatgt ccataaaaaa tctccttttg caattatgta taatttatgt ataccataca
    20701 aatactatat ttgtatagct gttttttgcc aaaatgctat acataagcta cccacgatgg
    20761 cgaaaaagtg atatcaaatt tttttctgag aaacgtgata ggcacagcct atctacccac
    20821 tttttttaga aagtgggtac acgtttcctt atgctcttta aaattcgggg gggagtgctg
    20881 cacgtgtaat cgataaccaa atttaacaga cgaaaaattg ttggaatata aaaatatgac
    20941 acccgaaaga aaacgatcgt tggaaaaatt aatgcaacta gtgaacgaag acggcggaat
    21001 ttcagacgca gcttttgacg catggagcga atttcatgga gaatataatt aagtgggttt
    21061 aaatggctct aaaatcgctt ctaagagttt tttagcttgg accaatgaaa tacaccaaaa
    21121 actaaataaa acgtcttaaa tcgaaaaata cgagctctca gagcgacttt atttgtaaat
    21181 gttgcatata agaaataatt acggatttta gacttttgta ggatattttt gaaataaata
    21241 tttatttctg ttataatttg attgcaaata agtgaacacg aaacacacga aatccccaga
    21301 gaggtcggat tctctggggc tttttttgtg ggctgagtcg ccttaattaa gttttacttc
    21361 ttgtttcggt tattcaacca gtaactgaac aaagcgctta aaagcccaac aagaagggag
    21421 caaataaagt tagtgaacac gaaacacacc tcccttccag tcgcaagact gtcggatagg
    21481 cgacaatcaa ttatacaata aaaaagcaaa aataagccta agttttttat tgccttgggg
    21541 ttgttagggc ttgccctgac cgcctgtaag gcgctttaga acgaaggaca tagtttttat
    21601 cgaatcttgt cttagaacgg cttagacgag ctctcaggac gccacaaagg agtccctaat
    21661 gaattatttt tggcgaagga gcctaaaggc gactgctgca agtaaaatgt gagagcgtag
    21721 cgatcactcc ttcttacagc gaccataggg agctagattg gcagtttaat atcatgcgcg
    21781 aacctgttta ttttaaaaag tgggtgattt tttggcggga aattttgctc gcgcgcgcgc
    21841 gtttatttta tttatttttt aaagattttt tagaaccctt tttagggggc agttgagcct
    21901 tactctccca acggatacag aagggtacta acaacttttt atctgagtac taacaacttt
    21961 ttatctgagt actaacaact ttttatctga gtactaatat ccttttgtat aataacaacc
    22021 aacctattta cgagttggtt gttaaaggtt attatttatt tgtaaggggg gaagaaagga
//
