LOCUS       NZ_LS992179             9294 bp    DNA     circular CON 13-FEB-2024
DEFINITION  Citrobacter freundii isolate Citrobacter freundii str. E2614 plasmid
            5, complete sequence.
ACCESSION   NZ_LS992179
VERSION     NZ_LS992179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4830817
            Assembly: GCF_900520335.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Gonzaga-Molto,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2018) Leibniz-Institut DSMZ-Deutsche Sammlung von
            Mikro, Mikrobielle Genomforschung, Inhoffenstrasse 7 B, 38124,
            Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:41:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,327
            CDSs (total)                      :: 5,205
            Genes (coding)                    :: 5,078
            CDSs (with protein)               :: 5,078
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 39 of 127
            Pseudo Genes (incomplete)         :: 82 of 127
            Pseudo Genes (internal stop)      :: 21 of 127
            Pseudo Genes (multiple problems)  :: 15 of 127
            Pseudo Genes (short protein)      :: 1 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LS992179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /isolate="Citrobacter freundii str. E2614"
                     /db_xref="taxon:546"
                     /plasmid="5"
     gene            join(9277..9294,1..360)
                     /locus_tag="CFE2614_RS27200"
                     /old_locus_tag="CFE2614_05395"
     CDS             join(9277..9294,1..360)
                     /locus_tag="CFE2614_RS27200"
                     /old_locus_tag="CFE2614_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(422..721)
                     /locus_tag="CFE2614_RS27205"
                     /old_locus_tag="CFE2614_05396"
     CDS             complement(422..721)
                     /locus_tag="CFE2614_RS27205"
                     /old_locus_tag="CFE2614_05396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(711..974)
                     /locus_tag="CFE2614_RS27210"
                     /old_locus_tag="CFE2614_05397"
     CDS             complement(711..974)
                     /locus_tag="CFE2614_RS27210"
                     /old_locus_tag="CFE2614_05397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(1280..1699)
                     /locus_tag="CFE2614_RS27215"
                     /old_locus_tag="CFE2614_05398"
     CDS             complement(1280..1699)
                     /locus_tag="CFE2614_RS27215"
                     /old_locus_tag="CFE2614_05398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2102..3787
                     /locus_tag="CFE2614_RS27225"
                     /old_locus_tag="CFE2614_05399"
     CDS             2102..3787
                     /locus_tag="CFE2614_RS27225"
                     /old_locus_tag="CFE2614_05399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3797..4054
                     /locus_tag="CFE2614_RS27230"
                     /old_locus_tag="CFE2614_05400"
     CDS             3797..4054
                     /locus_tag="CFE2614_RS27230"
                     /old_locus_tag="CFE2614_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            4139..4288
                     /locus_tag="CFE2614_RS27235"
     CDS             4139..4288
                     /locus_tag="CFE2614_RS27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5769..7733
                     /locus_tag="CFE2614_RS27245"
                     /old_locus_tag="CFE2614_05403"
     CDS             5769..7733
                     /locus_tag="CFE2614_RS27245"
                     /old_locus_tag="CFE2614_05403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            7733..8464
                     /locus_tag="CFE2614_RS27250"
                     /old_locus_tag="CFE2614_05404"
     CDS             7733..8464
                     /locus_tag="CFE2614_RS27250"
                     /old_locus_tag="CFE2614_05404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            8471..9001
                     /locus_tag="CFE2614_RS27255"
                     /old_locus_tag="CFE2614_05405"
     CDS             8471..9001
                     /locus_tag="CFE2614_RS27255"
                     /old_locus_tag="CFE2614_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9029..9208)
                     /locus_tag="CFE2614_RS27260"
                     /old_locus_tag="CFE2614_05406"
     CDS             complement(9029..9208)
                     /locus_tag="CFE2614_RS27260"
                     /old_locus_tag="CFE2614_05406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..8787
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4392..4970)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7734..8461
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agacaggcga cggaactgac gggtaaagcc cgaagttccc tgtatcggga tatggcgaaa
       61 ggccgcgtat cataccgtac ggaagctgat ggcggccggg tggtggatac cagcgagctt
      121 atccgggtgt acggtgagtt aagacaggat gagactcacg agcgggacga gttgagactg
      181 tcgggtgaga ctgaaaaaat ggcctccgac gcgttgatcg cggagataaa agcactcagg
      241 gaggaggtgg ccgggctacg gcaggaaatg cagtccatga gactgctgga acacaaaccg
      301 gagacagcag agaccccggc cggaacaccg cgccggtggt ggccgtgggg aaaacgctga
      361 cgcgctgtgc ttgtccaacc ggcgacggcc acgcaatgaa ttgcgtcccc gtcaccggtt
      421 attagagaac aatatgggga ttctggcgct ggccgaccac ggccagaacc cggacttttc
      481 tggtctggtc atcaacatca tagaggacat gctgtcccat cagactgatc cggcgaacgt
      541 cctcgccata gcgtgagtcc acttcataga gtcgcgggaa gtcggccagt ttgtccgccg
      601 cagcgagaac ccggtcggta acgttctgcg catcccacag ggatgaattt tccgcgatat
      661 agtcaaacag cgtcgttaca tcctcgatgg ctccctgctt ccattccacg tcatacggca
      721 tcgcgcagtt cctgataacg gccatgcagg cgctggcgga gttgttccgt ggtcataccc
      781 ggacgcgtgt cagcacgggc ggcggtcact ttggcgcgaa cctgatcttc atactctgcc
      841 tgggccagtt cttcgttagc cagtcgctgc aacagcgcag ctttgtccgg gccaccttcc
      901 ggcagtatgc ggggcaggct ttcagccagt tcccgaagaa agttggcgtt ttcgtagtgt
      961 gtagtgttgc tcattgcgga gcctcccggg ttaatgcggc aatatgaagg aagtttagca
     1021 gggggcggtc tgaaagaata tggtcgtgtc agtcaggctg atgaagcaga aagtcggtaa
     1081 aaaagggctt acggcatcca ttttacgtca aaacatatgc catgcagttg cgtgcatagg
     1141 catgcattag gataaaattt atcgggcgcg tttccggcag ttttccgggg gggttgttgc
     1201 ctgttttatc ccgtagccgc cggaaacgcc ctgagcccgt ctgagcggtg cgcgtaatga
     1261 cgcgttatgg taaatccact tactgtctgc cttcatagcc atcgagataa acagctgaaa
     1321 tggtgtcagc cagtagcctt gattttttcg gtgtgaggtc tttgctgcgg taatcggtac
     1381 ccgcaaattg tgttgtgcct tggagtgagt ttaaaaattc ctgactgtga aactgctgaa
     1441 tgcgttgctg cacatcactc cgtgattttc cggtcgcttt atcctttgcg cccatatccc
     1501 agaactgccg gaaaaaaggc accatcattc gtgttgtgtc agctttctgg gtggaaaaat
     1561 tgggatcaaa accgtcatct gaagcatata cgtaatgccg ggtatgctgt tccacgctgc
     1621 cctttggagc acaggcagtg agagttgctg tcgctgttat catggcgata agacacagtt
     1681 tgctgccgga acgtctcatg agcgtctcct tacctttaat tcttttttct cattgagtta
     1741 tatatccgca ggctatctta tcatcagttt ctgaccgtgt tcagtgaccg aacaacccga
     1801 accagacccc ccgtttcttt tcctgtcccg gagggggctt tctggtatct catataatga
     1861 tttcagtaaa acatactgag gccggagaac ggcgccgttt gtgctggttt ttgttgcatc
     1921 tggaggggat tgcgcatggt gtgagtggct gtttttttga tcattttcac aaaaacgcaa
     1981 atttacagac ttgacatgaa aaacaaggca gcgtaatttt tatactgtat ataaaaccag
     2041 tggttatatg tacagtattt tattttagtt tattgtttta aaagtcaaag aggaaaagta
     2101 tatgagtggc ggagacggac gaggtccggg taattcaggt ctgggacaca atggtggtca
     2161 ggccagtggg aatgtgaacg gtacatcagg taaaggtggc ccttcatcag gtggtggtac
     2221 ggatccaaac agcgggccgg gctggggtac gacgcataca cctaacgggg atattcataa
     2281 ctataacccg ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gcggtaacag
     2341 cggcaatcat agcgggagct ctggtggcgg acagtcttcg gccaccgcga tggccttcgg
     2401 tctgcctgcc ttggccactc cgggggctga aggactggct ttatccgttt ccggcgatgc
     2461 gttgtcggcc gctgttgctg atgtgctggc tgccctgaaa ggtccgttta agtttggtct
     2521 gtgggggatt gcgatttacg gagtgctgcc ttctgagata gcaaaagatg atccgaaaat
     2581 gatgtcaaaa atcgtgacgt cattgccggc agacacggta acggagacgc cggtcagctc
     2641 cctgccgctg gaccaggcga cggtcagcgt cactaaacgt gtggcggata ttgtgaagga
     2701 cgagcggcag catattgcgg ttgtcaccgg ccggccaatg agtgttcctg tggtggatgc
     2761 gaaaccgaca aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca
     2821 ggtgagcgta cctaaaggtg ttccggccgc gaaagccccg ccaaaaggca ttattgctga
     2881 aaaaggtgat tcacgtccgg ctggttttac ggccggtggt aactcccgtg aagccgttat
     2941 tcgtttcccg aaagagagcg gacagaaacc ggtctatgtg tcggtgacgg atgttcttac
     3001 tccggcacag gtaaaacagc gtctggagga agaaaagcgt cgccagcagg catgggacgc
     3061 tgctcacccg gaagaggggc tgaaaagaga gtatgataaa gcgaaagctg agctggatgc
     3121 tgaagataaa aatattgcga ccttaaacag tcgcattgca tcgacagaga aggcgatccc
     3181 cggtgcaagg gctgctgttc aggaagccga taaaaaggta aaagaggcag aggcaaataa
     3241 agatgatttt gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt
     3301 tcgctatctt gataaggata ttcagaatca gaatgagaaa ttaaaagctg cccagacatc
     3361 tttaaacgaa atgaatgaat ccttatccag ggataaggct gcgctttccg gggcgatgga
     3421 gagccggaaa caaaaggaga aaaaagcgaa ggatgcagaa aataaattaa atgaggaaaa
     3481 gaaaaaacct cgtaagggaa ctaaagatta cggccatgat tattttcctg atcccaagac
     3541 tgaagatatt aaggggttgg gagagttgaa agagggtaaa cctaaaaccc ctaaacaagg
     3601 tggtggtggt aagcgggccc gatggtatgg tgataaaaag cgtaaaattt atgaatggga
     3661 ttcccagcac ggtgagcttg aagggtaccg cgccagtgat ggcgaacacc tcggggcatt
     3721 cgatccaaaa acgggtaagc aggttaaagg gccggatcca aaacgaaaca ttaaaaaata
     3781 tctttaagag gtaaatatgg ggcttaaatt acatattcat tggtttgata agaaaaccga
     3841 agagtttaaa ggtggtgaat actcaaaaga cttcggtgat gatggttctg tcattgaaag
     3901 tctggggatg cctttaaagg ataatattaa taatggttgg tttgatgttg aaaaatcatg
     3961 ggtttcgata ttacagccac actttaaaaa tgtaatcgat attagtaaat ttgattactt
     4021 tgtatccttt gtttaccggg atggtaactg gtaagcaaaa gttatcaagg atgatttgaa
     4081 atacagactt ccggtgttca gggatgaata ccggggtttt taaatacagg gaaggtatat
     4141 gaaaaaggta aaggttgttt ttctttttat attgattttt tcaggctttc ttctggtcgc
     4201 atgtcaggca aactatatcc gggatgttca gggtggaacg gtggcaccat cgtcctcctc
     4261 tgaactgacg gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt
     4321 tttctgcgcg taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc
     4381 gaaggttaag tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc
     4441 aaccaaatct gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt
     4501 gtttagctaa acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt
     4561 ttgcgtgctt ttccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg
     4621 gctgaacggg gggttcttgc ttacagccca gcttggagcg aacgacctac accgagccga
     4681 gataccagag tgtgagctat gagaaagcgc cacacttccc gcaagggaga aaggcggaac
     4741 aggtatccgg gaaacggcag ggttggaaca ggagagcgca agagggagcg attcatcgga
     4801 aacggtgatg atctttaagt cctgtcgggt ttcgccactc ctgactgatt catggttgag
     4861 ccaccggctc ccacagatgc accgaaaaag cgtctgttta tgtgaactca gtcaggaggg
     4921 cggagcctat ggaaaaacgc cacctgcgtg gccttgccgt tttgcctcac atgttagtcc
     4981 cctgcttatt cacggaatct gtgggtaact ttgcatgtgt ccgcagcgcc cgccgcagtc
     5041 tcacggccgg agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctacaa
     5101 gacggacacc ggacgcgctg atgtggcata ttgtcccctg attaagtctt tatattccct
     5161 gataataaat taccaggata ggtgaatcat atcggttttt cgctgaacat tttacgttaa
     5221 cctatctctg ttgctttttt ctccttacgg agaaatacag gtcatccccg cgagggcgtg
     5281 gctgacctca gggggatatc ccctgcttac gctggctgga cgccaccgac gcacggtctc
     5341 tccccctctt tctggctccg tccggctgga cgccggccac gccagaaatt cacccggccc
     5401 ccgtcaggct cacgcctgac cacccacaaa aatccacggt gctggacgca cctggcgaag
     5461 cagcaaagct gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg
     5521 gggccggaac accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg
     5581 cgtgctcgtt gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct
     5641 ccgtggtgaa agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc
     5701 atgtccgcgg cttgtgcagc gggggctgca catcacgccg gcaaaccatc gtctgaagga
     5761 gagagaacat gtttaatacg gattcgcttg cctggaagtg gcacattccg gagctggccc
     5821 ggcagattta tccgtgggtc agctggctgt accaggagta ttacggtctg gccggggctt
     5881 tcctagttat cggcctgttt ttcggtctgg ctgaactttg ggccggacgg atgcgttttg
     5941 tggggctgag tcataaggag cgacgggagt atgccgatcg ctataagcgc gataataaac
     6001 ggccagcgcc attttcggtg ctggcctcgc tggattcgcc cgggcggatg gtcagggcac
     6061 gtcgacatgt ctggaactac gcgtactggc tgaccggcgc ggccggtctg gcccttctgg
     6121 gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc
     6181 tctacgcaaa cgcagattat tacctgctgc agccggctaa cctgttcggg attgcctggt
     6241 gcgcgggcgg gctggctgtc gggattgtgt tggcgcggct gctggcgttc ctgctgcttg
     6301 acggtcattt tgcggctgct gacgaggcgg tgaacgcgaa actgaatcag gagagccggc
     6361 gcagcagcca gcggacagga gagatgaccg acgtgcgcca cctgcatttc ggggagccgg
     6421 taccggttaa tgcgctggcc gatttttcct cggagcaggc ccggcaggag caggcggtgt
     6481 ttctgggtaa ggatgagcag ggcaagccgg tgctggttcc gcgtgatacc tggcgcaaga
     6541 ccaacattca gatcctcggc ctgcccggga gcggcaagag cgtgatggcc accaacgcgc
     6601 tcattcgctg cgtacgcgat ttcggggacg cggtggtgta cttcgacccc aaaggggacg
     6661 catgggcgcc gcacgtcttc cgggcgcact gtccggactt caccctgctg gatctgcggc
     6721 cggggaaacc ggcgcagctg aatctgttcc gcgatctcga ccagtacgcg ctgaaaaacc
     6781 tgctgattgc cgggcttaac ctgggcgaga ccggcgacgt ggcggaccac taccgcatca
     6841 gcgagcagaa agcggcgaag ctgattgcgg agcagtttcc gcagggggca aacattcagc
     6901 aggtgctggc ggcggcgtac gcgctgccgg aggccctgaa aaaggacgtg aaggggttga
     6961 tcactaggct ggaaaacgtc gctgacctga gcgtcctgca gacggacagt ggcatcgatg
     7021 tggcggggat tatcaacggc ggcggctgcc tgtacgttat cggctcgatg gacgacgagg
     7081 cggtgatccg tgtgcaaaaa atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg
     7141 acgagttccg ggagtggcca cacgtcagcg tgatgctcga tgaaattaaa tatctgctgt
     7201 cgaagtatgt actgaacgca ctggggacgc tgcgcagccg ggactgcaat ctgttgctcg
     7261 cgcaccagtc gctgggcgat ttcgggcagt gcgggcagga tctgcccgct gaatttgtga
     7321 aaaccacggt actggataac acgccgatcc gctggtttta ccgggcggcc agcctggagt
     7381 ccgcgcagtg ggcggcgggg cagaccgggg aaatcagggt ggacgtggag cgccgccgtg
     7441 ccagccggga ggcggggaac gtggagcata tcagcgggga cagcttcatc cagaaggagg
     7501 cgcggccgct gtttgacgtg aacaccctgc aacacctgcc ggacgggttc gcggtcatga
     7561 ccgggctggg tgtggcccgg atggcgttca gcagtccgct gcgggttgag cgccgggaaa
     7621 tcccgttgca gcgtttcccg gtgctggcga aaaccgatcc cctggcggag ttccggcccc
     7681 cggccgacgg cggcgggccg aaagcggatg atgattttgc ggggatttac tgatggcgct
     7741 ggagctgtac aacgtcagcc acgcgaaacg acaggcgcgg aacgcggaga aaacccggct
     7801 gaccctgcgc tggttgcggg aagagctgtg ttccacggcg gagctggttg cgcggcggct
     7861 ggggattgcg gcggtgcagc cggtctaccg cttcctcgac agcctggtgg cgaaagggct
     7921 gctggtccgg accaaatatc cggtggacgg ccgtcaggtc agcgtgtggg ggctgacgcc
     7981 gcatggcgtg gcgttctcgt tcgacgagga cgaaccgctg gcggatgtta ttccgtttca
     8041 gccgtcaagg gtgtcggcag cacatctgcc gcaccggctg gccgtgcaat ccctgcgcct
     8101 tgatatggaa gccagagggg ccaccggctg gcgcattctg caccgtctga gtcttaaagg
     8161 catgaaagtg ccggacgccc tggcggagct ggacggtaaa acggtggcgt ttgaggtgga
     8221 gcgcaccgtc aagagccgtc gccggtatca ggaagtggtg tccgggtatc tgttcaaccg
     8281 caaagctaac ggcatcgatg aaatctggta catctgcccg gaccgggcga cgcaggtcag
     8341 ggtgcagcgg gcaatcctgt cggtggatga aatcgtgaac ccacagaccg gcgaggcccg
     8401 caaaacggca gagctggatc gtgaacggtt gtttgcgtgc tttaaattta tgacaacgga
     8461 gtgagggatt atggcgctgt acatggtatt tattgaagac gcggaggacc ggaacggcga
     8521 gcgctgcatc atgccgacca gcgagcatct ggaagatatg gtggtgctcg cctggcagac
     8581 cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat
     8641 cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcactggtaa
     8701 aaatccgcaa tgggaacgga acaaacccac aggaatgaag ctgtgtgatt acctctatcg
     8761 caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc
     8821 accggagcag attaacgcga tgattgacgt ggtgagtgag cggcacgggg aaagcgcggt
     8881 gaacgagaag ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc
     8941 ggtacctgaa ctgccggagg acaaacctgc ccgccggtgg tggccgttcg gcaaaaaata
     9001 aatgccagca ccggccatgc cggtgctgtt attcctcccc gagcttatcc cgcatcacct
     9061 gccagagtgt ttccgcatcc tcgtgtagcc gctcgcacat gtcggccggg gtatccatat
     9121 ccagctcatt cagtttatcg agcaatagca gcgactgctc cttaataaat ttagccatat
     9181 tgagggctgt tttttcctgt tttgtcatga ttctttcctt ccgggcatgg tgtgcgcctg
     9241 tcaggtatac tgtctcaaaa atagcggggt gagacaatgg cgtgggtgac ggtg
//
