LOCUS       NZ_LR898885             1902 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_4I isolate MSB1_4I plasmid 12, complete
            sequence.
ACCESSION   NZ_LR898885
VERSION     NZ_LR898885.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357480
            Assembly: GCF_905071835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:20:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,157
            CDSs (with protein)               :: 5,157
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 84 of 283
            Pseudo Genes (incomplete)         :: 203 of 283
            Pseudo Genes (internal stop)      :: 41 of 283
            Pseudo Genes (multiple problems)  :: 37 of 283
            Pseudo Genes (short protein)      :: 3 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR898885.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_4I"
                     /isolate="MSB1_4I"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="12"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            68..607
                     /locus_tag="JMX41_RS27495"
     CDS             68..607
                     /locus_tag="JMX41_RS27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="WP_201555669.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKGGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQG"
                     /name="firmicute plasmid replication protein RepL"
     gene            614..901
                     /locus_tag="JMX41_RS27500"
     CDS             614..901
                     /locus_tag="JMX41_RS27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049092722.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     Typing          1513..1594
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="100.0"
                     /evalue="2.9e-32"
     Typing          complement(1140..1398)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.923"
                     /coverage="99.0"
                     /evalue="5.01e-120"
ORIGIN
        1 gttgtttcgt actgtacgat ataaatttcg taaggtgcga aatatcattg caggtaagga
       61 tgcaagtatg ttggaacagc aatttgaaga ctttgaacag caaaaacggc gagatcatca
      121 ggtacaagat caatccaaga acgctaagca aaaacgagat cggttgaatc ctccagactg
      181 gtcgcaggtt actaaggctg gttggaatat gttggacgct atatcagaaa aagttcctag
      241 agcatttcgg ttatattctt tttttgccca gaatatagat aaatcatgtg gggctgttgt
      301 atgtgatcaa aaattcttag ctgattattt cggtgttagt ttaagaacga tccaatattg
      361 gattggagat ttagaaagta ttggcgcaat agttaaaata ccagtaggca tatattatgc
      421 ctatgcatta gatccttatc aggtatggcg tggatataac aatactaaaa aatatgcagc
      481 atttaatgca aaaacattaa ctcaaaaagg tggggttata gatcgtaaat tgcggcttat
      541 gctttctagg cgtgaagagt ttgaaagttc cttaaagtac tctaatcatg actcagagca
      601 ggggtgatgc actatgagaa ttaaatatga atcacgttgg agattagaag aactaaaatt
      661 agaaatatgc agttcaatag atgagttaat taataaatat ggtattgaat atgcaacagg
      721 aataaatctt tatatgaatt gtgaagataa agaatttaat cttgttgatg ttatcaatga
      781 agatggaaat aaaataatgt ttgtcgttag caacgatgaa aaaatacaaa agccaaaaaa
      841 aatagcaaag aaaaaaactc cagcacctgt aattgagcta gataaatatc gaaaaaaatg
      901 accattgagc cagcggcagg cggacaatca ggggctacgt gttaacgttc tgaccttgat
      961 tgtctatcct gcattgctct tttgccgcct cgaatccctt gcgtgttttt gttccacgct
     1021 ctccagaacg accgagccgc cacggttccg atagcgcctt gagcgcaacc ggaaaccgtc
     1081 tggggagaac cccagacccc catcgctgta ataccgacgg tctacgacca cctaccagcc
     1141 gaatagctgc ctatgccgtg caaataggct ttgcaggggg ctgacgctgt tcctggcgac
     1201 ctgaaagccc ctggttaggg gtatcggcgc gcctgacctc ctgagagcct ctgtaagcgt
     1261 ttttcgcgcc ttccatgccc tgacgggcat ttaaaacccg tttcgtgcgt ctgagagctt
     1321 ctgagcgcgt ttgaagggct atctgacgac tcatccctga aagaacaaca acgggagcac
     1381 cgacaacatg cgagaggtga caccgctcgc cgcaagtcga tgaccgagcg tagcgagcga
     1441 atcgacgagg aagcggaaga gaaccggaag ccacattgag cacttacgca ctgatgcggg
     1501 gtgtcggggt gaagccctga ccaggtggtg aatgtctgag tgcgcgtgcg cggtccgaca
     1561 ttcccacatc ctgtcccgat ttctgaggct ttttagcggt gaacggacgg caaaaccatg
     1621 atggagttgt tgaacgttga gagcatgaaa tctggcaaaa atcacggctg gtaaggcttg
     1681 cagaggagta agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc
     1741 cgacacccca aaagcagtta ggtagtgtta aatcatgagc agtaaaaaaa cgctttgata
     1801 gatctttaga gcaaaaattg acatttcttt aaaaatcatt gacttaagac accttcatac
     1861 gaaacgttat ttgcatgtgt atacgcaaac atgtttgcat gt
//
