LOCUS       NZ_LR898883             4072 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_4I isolate MSB1_4I plasmid 10, complete
            sequence.
ACCESSION   NZ_LR898883
VERSION     NZ_LR898883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357480
            Assembly: GCF_905071835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:20:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,157
            CDSs (with protein)               :: 5,157
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 84 of 283
            Pseudo Genes (incomplete)         :: 203 of 283
            Pseudo Genes (internal stop)      :: 41 of 283
            Pseudo Genes (multiple problems)  :: 37 of 283
            Pseudo Genes (short protein)      :: 3 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR898883.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_4I"
                     /isolate="MSB1_4I"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="10"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            join(3667..4072,1..188)
                     /locus_tag="JMX41_RS27470"
     CDS             join(3667..4072,1..188)
                     /locus_tag="JMX41_RS27470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012537770.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="hypothetical protein"
     gene            complement(199..390)
                     /locus_tag="JMX41_RS27965"
     CDS             complement(199..390)
                     /locus_tag="JMX41_RS27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225879636.1"
                     /translation="MCLMGTVCAQTVRHSFSCIAMQWAENDPDAFFRFSLGKTSLFAVS
                     ASESRSVRFSGARNATLW"
                     /name="hypothetical protein"
     gene            961..1155
                     /locus_tag="JMX41_RS27475"
     CDS             961..1155
                     /locus_tag="JMX41_RS27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1820..1933
                     /locus_tag="JMX41_RS27970"
     CDS             1820..1933
                     /locus_tag="JMX41_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531573.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="replication initiation protein"
     gene            2144..3073
                     /locus_tag="JMX41_RS27480"
     CDS             2144..3073
                     /locus_tag="JMX41_RS27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_251123212.1"
                     /translation="MIEMDMPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYI
                     WSQKASSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHGDFIHNINKA
                     KKRYENEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEASRALWPR
                     LTYRVLKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIR
                     EKINKIEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTIL
                     DQLYALTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     Typing          complement(447..702)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.023"
                     /coverage="89.0"
                     /evalue="1.24e-101"
     Typing          complement(827..925)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(1236..1321)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          1334..1892
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.187"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caattataag ccttcgttat cagtcaaata atactgttaa ggtgacacat gaaacactga
       61 gaagctatac aggtgtccag ccaaaccaga ttaggttcgg cttagatgta ctgtactgta
      121 gtcgcctgat acatctgcaa ccaaaagaag atagagagtc aaacatctat gaaataatcg
      181 gcctatgagg aaatgcattt accataacgt cgcattacgc gcaccgctaa aacgcactga
      241 acgcgattct gacgcagata ccgcaaaaag agatgtttta cccagtgaaa accgaaaaaa
      301 tgcgtcagga tcgttttcag cccactgcat agctatgcat gaaaatgaat gacgaacggt
      361 ttgggcgcat acggtgccca tgagacacat tttcggacat tttcagttcg tgaatccctg
      421 actcattgct acatatctga acagacaccc ctggcagcgc ccccaggggc gtcctgtaaa
      481 aaaacacacc gcgccgctag cagcacccct gatataaaat gatgtttttt ataaaaatag
      541 tcagtaccac ccctacaaag cggtgtcggc gcggtgttgt agctgcgccg gcaccgcttt
      601 tttaaatatc gcaaagagag caagagaaac taatttttca taactatcta tttataaaga
      661 aaaatcagca aaaacttgtt tttgcgtggg gtgtgggcat ttcaggaatg agaacagact
      721 cgcgtttttc cggagtatca gcggaattat tgattaagaa acactcgacg cagagcgaaa
      781 ttttatgcaa cagcggctgc actgcacagg gattatttat cagttgatat gctgtgccat
      841 gatacgtcct aaaaaaacag gacaggatgt gcaattgtaa taccgtcgcc cgcaacgtct
      901 tacaattacc acctgctcag agtccccccg acacccggaa ctgacggaga acaacggaag
      961 gtgaacaaac aacaacaaac tgcgctgaat atggcgcgat ttatcaaaag ccagagcctg
     1021 acactgcttg aaaaactgga tgctctggat gccgacgaac aggcggccat gtgcgaacga
     1081 ctgcacgaac tcgcggaaga actccagaac agcatccagg ttcgctttga gaccgaaagt
     1141 gaaacaggaa cataacgaag ctgacggagg cgctcacggc ttgtccgcga gcgttttcgg
     1201 cagcagacaa gcccgtcagg gcgcgtcagg gggttttagc gggtgtcggg gcgcagccat
     1261 gaccaagtca cgtagcgata gcggagtgta tactggctta accatgcggc atcagtgcag
     1321 attgtatgaa aagtgcccca tgccgggtgt gaaattccgc acaaatgcgt aaggagaaaa
     1381 tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     1441 ctgcggcgag cggtactgac tcacacaaaa acggtaacac agttatccac agaatcaggg
     1501 gataaggccg gaaagaacat gtgagcaaaa gaccaggaac aggaagaagg ccacgtagca
     1561 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatagac gctcaagtca
     1621 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1681 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1741 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt
     1801 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1861 cggtaactat cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac tggcagcagc
     1921 cactggtaac tgatttagag gagtttgtct tgaagttatg cacctgttaa ggctaaactg
     1981 aaagaacaga ttttggtgac tgcgctcctc caagccagtt accttggttc acagagcgcc
     2041 aactctttga accttcgaaa aaccgcccgc caaggcggtt ttttcgttta acactctaca
     2101 actatccata cgtgtatggc aacatccaat caccactaag tgtatgatag agatggatat
     2161 gccaaataca attcattacc ctcatgtaat accatttata tcccaaggaa aaataaatgc
     2221 tataaaaagc acgtttggta ataacctgtc tgatagggag tgctacggga tatatatatg
     2281 gagccagaaa gcctcatctg ccatatatcc attactgcaa caactcgaag ttactttgag
     2341 aaactcgatt gataaagaag cgacaaaact aataggccaa aaatggtggg ataatgtata
     2401 caccgacaca tcaaaaagca aacacggtga cttcatacat aatataaaca aagcaaaaaa
     2461 aagatatgag aatgaattca agaaaaaaaa cccatcaatg gcaaatacca aaataattgc
     2521 acctcatgat gacatcattg cacacacaga tttttacaca tggcaagcgg ttttgagcga
     2581 tgcttttcat acacaatcaa gatctgaagc atcaagagcc ttatggccca ggctcaccta
     2641 cagagtactg aagggattag atagaagcaa agatgaaggc acagccagaa ttgatttctt
     2701 aaacgagctg aatgagatca gaaactacag aaataggtta tcgcataatg attgcatatg
     2761 gataaaaata cactctaaaa atttgcaatc tgcagtagaa acaataagag aaaaaataaa
     2821 caaaatagaa ggactgataa aaacgataaa cccacaggta catctatcat taacgaagtg
     2881 gggttccttt tatcacgcca aaagaatatg ttctcaaaaa gaggccgaat tgcatctagg
     2941 caaaggaata atcaatagca caacagatga gatgaacaca attctagatc aactatacgc
     3001 actaactgct gacggaaagc taacgggtgt tgtaaaacga aataccaaca atatcgcatt
     3061 tcacaagttt tagatcacaa aatagaaaag gcccgacagt ttcctgtcga gcctcatcca
     3121 aatctcttgc cattatcacc tttcggtgga gcgtggcctt aatgtcattg tgaatctata
     3181 aaaatttcac aatgcattaa agcgaaagct caaggcttca ctgccataca tgatacatca
     3241 tcatcaggaa aaagagcaaa ctatttccca tgatcacgcc aaagcttacc atccaccatt
     3301 taccaataga tcaaaaaaca cacaacaaaa ataactaaaa acgccgatta aaccagcatt
     3361 atcaaattta catttaccag ttaaaaaacg agaaaaacaa gataacatct tgttttatat
     3421 gggaaaacaa gcagacacca aatattgctt tatctagatt tattctaata agcgtatata
     3481 aagttaaaac acaacaactt agtatgcgac tggaggacca attggaaacc ctcaacaatt
     3541 tgctggtaac gctaacttcc gcaaagccgg aggttttggt ttctgttgta tctttaggtg
     3601 caatggtttt catgtatatg ataataaagc tatttatatc tctccttgac cgaaaatggg
     3661 gtaaaaatga gcggtagaaa atttggcagg cttccaaact ggtgggcccg aagctcatgg
     3721 cttgtaaaca atgttcgttc aaatgagata gggggcggca ttgcatcaat gaaatgcttg
     3781 ttagctctat ccgtcttgat tgatttccat tcaagaacag cctcagtatc atttaccgga
     3841 atggaaagac tgactggact aagccgcccc atgatagtga aaggagtagc caaacttgag
     3901 aaagatggtc ttataaagat agatagagag atgtttgtta actcctatga attaacagta
     3961 cagccaaatg atgataggtg ggcaaaagta ccagtagata ctatcaggaa aaaactaaac
     4021 accatatcga acagggggat ggccccattt gtggcattaa aactatattt aa
//
