LOCUS       NZ_LR898882             4082 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_4I isolate MSB1_4I plasmid 9, complete
            sequence.
ACCESSION   NZ_LR898882
VERSION     NZ_LR898882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357480
            Assembly: GCF_905071835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:20:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,157
            CDSs (with protein)               :: 5,157
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 84 of 283
            Pseudo Genes (incomplete)         :: 203 of 283
            Pseudo Genes (internal stop)      :: 41 of 283
            Pseudo Genes (multiple problems)  :: 37 of 283
            Pseudo Genes (short protein)      :: 3 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR898882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_4I"
                     /isolate="MSB1_4I"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="9"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            complement(332..586)
                     /gene="mobC"
                     /locus_tag="JMX41_RS27455"
     CDS             complement(332..586)
                     /gene="mobC"
                     /locus_tag="JMX41_RS27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            765..2924
                     /gene="mobQ"
                     /locus_tag="JMX41_RS27460"
     CDS             765..2924
                     /gene="mobQ"
                     /locus_tag="JMX41_RS27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_201555667.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMKTRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3180..3881)
                     /locus_tag="JMX41_RS27465"
     CDS             complement(3180..3881)
                     /locus_tag="JMX41_RS27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(588..764)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          765..1123
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3181..3881)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          679..2921
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.733"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttctataa ggaattttgg cgctaaaaca agtattaaaa acaagatctt aaaaacaaga
       61 tctaaaaact taacgacgag cctgtggata acttggagtg ctgaaaacaa aaaaccaata
      121 aaaacaatgc gttgcaaaaa agcagaattg caagttccga atacaccccc tccaaccctg
      181 tggaaatcca gtattcatgc gggttcagcg catatcacct cccgaaaaac cgtgcgtatg
      241 tgctcaatca ggcgacggat tcacttccgc ttcctcgccc actcgctcgc taacgctcgg
      301 tcgctgggac tgcggcgagc ggacgataca atcactgtcc tggcagcgct tccagctcgt
      361 taatcagcgg cagtatgcgc gtcacatcag cctgtcgtgt cacctctgcc ttcaggaact
      421 ggagtaacca ggcacgcaac tgcacctcct ccggctttct gacctttgcc agcaatgctg
      481 cacccacaat gaccttctgc gctgtgtcca gactcctgct cttcgccttc aggcgctgta
      541 atctggcctc agcatcagca atcttctgtt cgagtgttct gctcatgatg taactccgtg
      601 cgcgatgaaa aatcgcattt tagcgcgtca ctggtagttt aaaaactgag ctggcataat
      661 gcacggaaaa tcacgaagtg cgcacttata caatctccac tccgtttcga ttgtgtgcgc
      721 cctgcggggc tgaaagaaaa ccacaaaaag gcattacggc agaaatggcg atttatcatc
      781 tcagcatgaa aatcatctcc agaaacagcg gctacagcgc cgttgcttct gctgcctatc
      841 gttccggttc actgatgctt gatgagcgta ccggactgac tcacgactac acccgcaaaa
      901 gcggtgtggc tgaagctgtg attctgactc ccgcgacagc ccctgcctgg tgtacgaacc
      961 gtgccgaact ctggaatgcc gttgagaagg ccgaacgccg gaagaactca caacttgcgc
     1021 gggaaattga actcgccatc ccccgcgaac taccgcagga tgccgcacgg gaaaccgttc
     1081 tcgcctttgt cagagaaaac tttgtcagtc agggcatgat tgctgatgtg gcgttccatc
     1141 acatggataa aacaaatccc catgcgcaca tcatgctgac cacgagagct gtcggacctg
     1201 ctggattcgg tggaaaggtc agggactgga acgaccggac acatgccgag acgtggcgtg
     1261 catcatgggc tgaccatgcg aacagggcac tggcgaacgc cggttatcag gaagagatag
     1321 accatcgttc atacgagcga caggggcttg agaaaacgcc tggtattcac ctcggaaagt
     1381 ctgcctgtgc gatgaaaaca cgcggtatcg aaacagaacg cggcgaacag aatcgtctga
     1441 ttaacagact taacctggaa atacagattt cccgtaccga actcagaaac agcgggctga
     1501 cagagcctgc ccgccgtgtg gctgaccttc tgaatatcgt gcttcctgac aacgcaactt
     1561 cgtacacact acgtgacatc atcgaagcgt tgccgaagga cagtaatgcg gcatggaaac
     1621 tcaccagtaa tgccatgtcg atgatggcag atatgcaggc gacgcgcaga cgctgggaag
     1681 agctgaacac cgaacgaaag gaagcgatga gccatgctgc cagcctgaaa aaatcatcgc
     1741 cgttttcgtc cggtttttcc cgtattcccc tgatgcaatg ggcggcaccg gaataccgta
     1801 gcgaacaggg aaaaatccag tcgctgagcc agcaaatgga aaaattgcga cagcattacc
     1861 ggaaggtgga gaaacaggac attcccgcga gtcagacggc atttgaaaaa cagtggcagc
     1921 agtggggggc atccggcctt gcagctctca gagtccagtt gcttaaacgt gaagaggaat
     1981 tgcgccggaa ggagcaggag gatacagaac gtagtcgcgc ggagcgacga gcggaacagc
     2041 tcaggaaaaa tgacaatgcc gttatggatt caggggtact gagcgcagtc gttacggggt
     2101 atggcgaggc ccccatgcct ggtaacgggg aaatgacctg ttaccttctg cttcacaacc
     2161 ggaacggaga atacacgctc tgggggaacg agctggagaa ataccgcacc agaatctttg
     2221 agcgtgttga tttaatgcgt gacaggagtg gttatatctg tgaccgaagc gaaatcggtc
     2281 aacatccacc actgcaacgt gtttattcct ctgcgacctt tgagcaactt ctgacgcagg
     2341 tttgccagac atggccgcaa tatacccgca atctccgcca gccgaagaca tggccggagt
     2401 ctttctgtct tggagaagac cgccagccag ccatgccatc actggcagca aggaaggtgg
     2461 attttacgca gggacgtctg ctaccgacgc tgatgccggt aatgagttct gtcgaccggg
     2521 aaacccgcca gcttcagctt ttgctggtca tgggcgtgga tgactctctg ggcggtgttg
     2581 tccggcttaa tggtacgctg tatcccgcgt ttgccgttcc ctcagccgat aacagccagc
     2641 tggtcatcag tgcactgacc gataaggggt tacggtatgc agggtatggt gttgccgtaa
     2701 atcacgatgc agacagccac atatcacctg cgccggaact tatggagttt catctgaaaa
     2761 cccgtgaggc accgcttttt gctgccgtca acacaccaga aaaacagccg gatcatctgt
     2821 tccgctcgct gggttttaat cgtacctggg acgaatggcg gcgcgaggaa gatgcacgaa
     2881 cacacgccac cgagaggaga catgatcgcg gttggtcaca gtgaatgtga acaatatttt
     2941 gttttgttta acatcgtgtt atgttcgtcg atgttcttcg atgttcatcg acagggatga
     3001 atgcaagaag cacacacata atgcaagcat agttttgttt ttccgccccc aaaaaatggg
     3061 ggttcttttt tcgctgcatt agagcgcttt tgacgcaatt tttcacttca atagtacctt
     3121 cgcactacta acacaaaaaa aacgcagcag ggagcgtttt aggtgcctta aaatcgattt
     3181 tagatcgtca tcttgatttg tttgtcttcc ttgaagcgaa aatgcagggc tacaaccgtg
     3241 cgcccctttt tgatcgtctc aaccgttaca gccaggtcgc tttttttgtt cagttcgtca
     3301 atgcagggtc ttaataattg ctggttaagt gatttatatg tttgatattt atgctctata
     3361 tcaagcattg agcgaaaatc atcgagagcg ataatacgtt cgccagtact gcggaactgc
     3421 tggaggagtt cgtatatcct tatactgtaa cttcttgata atctagatat attcttgatt
     3481 acaactctag taaattgccc ttgtagttgt gtcaaataag gcattactgc gtcagaaaat
     3541 gttatttgtg ctctggcctc gcctttagcg tactgtgccc gatactggat ccagcggaac
     3601 tcctcacgtt tctcgtcatc cttgagaatg attgacctgt cccacaaacg atcaattgct
     3661 tcctgtaact gaacctcggc atttttccgc cccatgtcag ggaatgaatc gaaatattcc
     3721 gcagctgtaa tggtcattgt cttttgcaat tttggagatt ctgcatcagc cccagacttc
     3781 aatcgaccga tgcaaagtaa caaaaatcgc tgttcctgta gggtcaattt ataactggct
     3841 tcaaccagcg cattggattt ataggcaatt agatccgaca tagagcatcc ctccccaaaa
     3901 ggtaatgaat aaaagataaa cataccctca acgaaaggca atgccttttt attttaatat
     3961 ccctttttct gtgaatatta ccttaaatca tattgccttt accttaaatt cccccaccta
     4021 aacaccttaa atcatattac ctttacctta aatcacatta cctatagcat tgtaacattt
     4081 tg
//
