LOCUS       NZ_LR898881             5180 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_4I isolate MSB1_4I plasmid 8, complete
            sequence.
ACCESSION   NZ_LR898881
VERSION     NZ_LR898881.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357480
            Assembly: GCF_905071835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:20:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,157
            CDSs (with protein)               :: 5,157
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 84 of 283
            Pseudo Genes (incomplete)         :: 203 of 283
            Pseudo Genes (internal stop)      :: 41 of 283
            Pseudo Genes (multiple problems)  :: 37 of 283
            Pseudo Genes (short protein)      :: 3 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR898881.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_4I"
                     /isolate="MSB1_4I"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="8"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            join(4288..5180,1)
                     /locus_tag="JMX41_RS27430"
     CDS             join(4288..5180,1)
                     /locus_tag="JMX41_RS27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_021536792.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQP
                     YAQHWLAFDVDRMGAAIDWSDRNCPPPNITVKNPCNGHAHLLYALWLPVRTAPDASASA
                     LKYAAAIERSLCEKLGADVNYSGLICKNPFHREWQVAEWREDAYTLDELADYLDLSASA
                     CRSIDKNYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSVAKYTHRKFSPEGFSAVQAARGRKGGTKSKRVAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(205..603)
                     /locus_tag="JMX41_RS27435"
     CDS             complement(205..603)
                     /locus_tag="JMX41_RS27435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039720373.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQSLKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            779..1705
                     /locus_tag="JMX41_RS27440"
     CDS             779..1705
                     /locus_tag="JMX41_RS27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_149068890.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIGRKGEYAKRKDLEYMEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1708..2241
                     /locus_tag="JMX41_RS27445"
     CDS             1708..2241
                     /locus_tag="JMX41_RS27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2295..2516)
                     /locus_tag="JMX41_RS27450"
     CDS             complement(2295..2516)
                     /locus_tag="JMX41_RS27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001679939.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLRKLPPDHTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     Typing          4289..5180
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.776"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          780..1702
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.156"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaccagata agccttatca gataacagcg ccctttcggc gtctttttga gcggcgccct
       61 ttccggtgtt tctctcagtc cagctccgca atggcgcggc ttttcgcatg attgtgctgg
      121 tagtgctcgc cgcagtctca tgaccgagcg tagcgagcga atgagcgagg aagcaccagg
      181 gaaccggaac acggatggag gcacttacgc gtctgggttt ggggatgtct ccagatcgta
      241 cttaatttcc ttaagtgact gtaatatcct gtctttttta tagcatagct cgcctgtttt
      301 tgtgtctacc agtcctgctt cgatcagtag cccagccgcg agaaagagtc ggtgattgcg
      361 ttcccgtcgt tgtttttttc cctcgattcg tttttggacc gcctgttttc gttcgctctc
      421 ctgtgcctgt aagtcgtttt tttccgctgt tgccagtacg tggaggagtt tgcgttcctg
      481 ttctgtgagt cctgcttcgt tgtcgagcag gtcaataatc tgtctctgtc gtgcggtctg
      541 ttctccgagc ttcctgatga atccgatccg tttcttcagc cattcgatgt tgctgatgcg
      601 catgttcgtg gtgctccctg gtgttttttg ctgtgtgttg tggtcgtgca gtatatcgct
      661 tgcggtcggt gggtgcaggg ggtatcttcc gtgctaattc gtaaaattag aaggaagcgc
      721 acttatatgt ttctctcctt cgtcgagaaa cagtgcgctc ctgcggaggg ctgaaaccgt
      781 ggcaacctat cacctcagcg ttaagtttgg aggtaaggga caggctgcaa accacgccga
      841 ttacatcggg cgcaaaggtg agtatgcgaa acggaaagac ctggaataca tggagcatgg
      901 gaatatgccg gaatgggcgc gagataatcc ctcccatttc tggcaggcgg ctgaccagtt
      961 tgaacgtgcg aacggttcga cttatcggga actggaaatc gctctgcccc gtgaactgac
     1021 agcggaacag cgccttgaac tggtgcagga ttttgtacgt caggaagccg gcgaacggca
     1081 cgcctggagt tttgccattc acaatccgaa agcgagcatt gacggcggcg aacagcctca
     1141 tgcgcatatc atgatgagtc agcgtgtgaa tgatggcatt gaccgtacac cggaacagta
     1201 tttccggcgc tataacgcca ggtatcccga gcggggaggt gcgaaaaagg attccggaag
     1261 cctgactccc actcagcaaa aagagcagtt gcgggaattg cgaaaacgct gggaggtgaa
     1321 gcacaacgaa catatgagaa agcatggctt tgaaaggggc ttcattgact gccggacgct
     1381 gaaagaacag ggcattgaac gacgtcccga agtgcatctg ggttttgaag ctgccggacg
     1441 acttgatgat gcccagcgtc atgacatcat gcaaaaacgc tctgaaccgg actattaccg
     1501 ccatcttgaa caggcgcgaa tggtcgggca tgacatcagg gaatggctgg atacaccggt
     1561 taagcaacat gaactggtca gaaggctgca acatgatgtt gcgcgggagc atggctttga
     1621 catgagagac ggagtgagca gagagaaccg aactgaacct gaacgggtta aatcaccgtc
     1681 acgtggattt tctatgggat tctgaacatg gccagcacag aagaaaaatt actcacgctc
     1741 ctgttatcgg tggagcatct gcaacatacc gcaatggagc aacagaaagc tgttgctgat
     1801 tctctggctg tggcaaggga aacacttcgc ggatatcggg atatttctga cggtgtggca
     1861 aaacaggtca gggatgaagt cagaaatgag gtgcagaagc tggacgtggc aggactgata
     1921 ggtgagcgca tcagccagga ttttcagcgg ctggaagagt cagtcgcaaa catgaaggca
     1981 aacgcggatg ccctgaacag ggatattgcg acaattcaca gcaatatcgt gcaggaatac
     2041 aacaccctta aagggtatag ctggaaatgg ctgggtgggg tgtcagtggc ttttctggtt
     2101 atgttctgtg tgctcggatg gggaatgaaa acaatactcg acaggaacta caacgccaca
     2161 gcggcggtgt atcagaaagt tgtgacactg gaagataaac tggtggagaa acctgtcacg
     2221 gggaagagcc ggaaaaactg acgcgcttcg cttgtccaac gcctcacgga gcgcgaaagg
     2281 atttcgcgaa ccgttcaccg ttttttccaa tatgattctg tctgctttgg tcgataaagc
     2341 agtcgatagt attccagcct tcgtatatat aacggtacgt gctcatcagt aagatctggc
     2401 ggtattgtgt gatcaggggg aagttttctc agatactcac gaaatgcctg gcttgatgcc
     2461 ataaaatcta cagtagcatc gataatatgt ggggtgtttt cacctaattt tgccatgatg
     2521 ataatcctta tattatcacg gttcgtgaat gatagggtgt cactattatt tatgcgttag
     2581 taaaccagaa aaaatccccc gttggcatag ggggatgtat aaggagaaat aacttcgcag
     2641 gtgttcttgt ttgtataacg tgaaaagact gcacgcaact taataaatat gccgtactta
     2701 ctttcagttt tcaacataag gtttataaca aagtatgaaa tagaactgag ctttaaggtt
     2761 tcagctctaa aaaataaaat atttattagt tggttggttg ttgttcttgt tctgtatgta
     2821 tataaacact atcgacatat tatgaccggg aatatttctt accccttctg cgctctcaga
     2881 ggggtttttt ttgtcattaa ttaataatga gctgaaatgc agttcagata cctccaattg
     2941 cacattatcc ctgacatatg gtaccggaac cgcattaatt ttatgttata agagtttatt
     3001 tttaatgtcc agatggttta ataagtgttt atatgtgatt agctgtatga ataatactgg
     3061 ttttattatg taacttgttg tttttattgt tttttcatgt ggtgtgttaa tctttaagtt
     3121 ataaaaaaac cccttggaac acaggggcaa aagtaaggaa aacaaatgga tgctcagttt
     3181 gtgtgacact ttaaactaat ggaactatag agtggaggtt gttacattcg tttttataaa
     3241 tcagcaaact gtttttttct atagcctgat gataaatggt gacaattttg tgatattgtt
     3301 caaaatgctt tgagtaaaat gtttgttttt tagtctattg gtcggttttt tttgttagct
     3361 ccatcaagaa gtgtgattga tgtcgcaaga gtggaatgtt atgaatataa actgattatt
     3421 tagtaaatct aacttgctta gcaaaatgtt cttgttttgt ttgtgagttt ggtgaattta
     3481 tctgtctttg tgtttgtttg tttttataaa atcatgcaca atgatcttaa attgattgtt
     3541 gaggccggat tatctgtgcg gtaattatta tttataacgt aacggcttca tcttcatcgt
     3601 tgttcgaaca caacaaacaa cctgtaattg ttgtttctct tggttcccca cttttgtggg
     3661 ttttttttat ctcttccgtg catcgtaaag cgcggactcc ttctggaaag gttgttttag
     3721 ggcatcactg aggcattttt tgcgtttgtg aatattgagc atccgataac taaaatattt
     3781 cccatagact ctgattcttg ccaccgaccc caggtcggtt tttttttgtc ctggctagat
     3841 gtgaagtttg gaaacaggaa actccgtcag agcgccacgc accaggggag aaaacttgtc
     3901 attttgaccc gtccggtgtt ctgaacggtc gaaaccgatc gcgcatcgct ttcgtgcata
     3961 gttatgcaac gcgctaaaaa cgattctgac gcatttttta tgtttaccct ggtgtttcct
     4021 tgtctttttg cgttttttgc gtcagaaagc gactgagggc gttttaaggg gggcgtacaa
     4081 cgggagttat ggtaaatgga tcggcttttc gggaaggatc gacaggattt gccgttgggt
     4141 gtagtgtaag cgactgaaaa acaaacgccc cgtaaatcgt gctctcaccg ccaagattga
     4201 ccacgaaatt acagggcgct gggttccgcg tctcccgatg ggaaagcgcg gttagttaaa
     4261 ctgtgttccc gataggaatc gtatcacatg agcgccgtac ttcaacgctt cagggaaaaa
     4321 ttaccgcaca aaccgtattg tacgaacgat ttcacgtacg gcgttcgcat tctgccgaaa
     4381 gacattgcca tccttgcccg cttcatccag cagaaccagc catatgcgca gcactggctt
     4441 gcctttgacg tggaccggat gggagctgcg attgactgga gcgaccggaa ctgtccgccc
     4501 ccaaacatca ccgtaaaaaa tccctgtaac gggcatgcgc atctgctcta cgcgctctgg
     4561 cttccggtga gaaccgcgcc ggatgcatcg gcttcggcgc tcaaatacgc tgccgcgatt
     4621 gaacgttcgc tgtgtgagaa acttggcgca gatgtgaatt acagcgggct gatttgcaaa
     4681 aatccgttcc accgggaatg gcaggtggct gaatggcggg aagatgccta caccctcgat
     4741 gaactggcgg attatcttga tttgagcgcc tcagcgtgcc gtagcatcga taaaaactat
     4801 ggcatggggc gaaactgcca cctgttcgaa atgacgcgca aatgggctta cagggcgatt
     4861 cgtcagggct ggcctgtatt ctcacagtgg cttgatgccg tgatccagcg tgtcgaaatg
     4921 tacaacgcat cgcttcccgt tccgctttca ccgcctgaat gtcgggccat tggcaagagc
     4981 gttgcgaaat atacacatag gaaattctca ccagagggat tttccgcagt acaggctgct
     5041 cgaggtcgta agggcggaac taaatccaag cgcgtagccg ttcctacatc agcacgttcg
     5101 ctgaagccgt gggaggcatt agggatcagt cgagcgacgt actaccgaaa attaaaatgt
     5161 gatccagatc tcgcaaaatg
//
