LOCUS       NZ_LR898880             5210 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_4I isolate MSB1_4I plasmid 7, complete
            sequence.
ACCESSION   NZ_LR898880
VERSION     NZ_LR898880.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357480
            Assembly: GCF_905071835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:20:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,157
            CDSs (with protein)               :: 5,157
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 84 of 283
            Pseudo Genes (incomplete)         :: 203 of 283
            Pseudo Genes (internal stop)      :: 41 of 283
            Pseudo Genes (multiple problems)  :: 37 of 283
            Pseudo Genes (short protein)      :: 3 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR898880.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_4I"
                     /isolate="MSB1_4I"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="7"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            complement(<460..891)
                     /locus_tag="JMX41_RS27960"
                     /pseudo=""
     CDS             complement(<460..891)
                     /locus_tag="JMX41_RS27960"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1100..1423)
                     /locus_tag="JMX41_RS27395"
     CDS             complement(1100..1423)
                     /locus_tag="JMX41_RS27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1488..1679
                     /locus_tag="JMX41_RS27400"
     CDS             1488..1679
                     /locus_tag="JMX41_RS27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2867..3256
                     /locus_tag="JMX41_RS27405"
     CDS             2867..3256
                     /locus_tag="JMX41_RS27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(3320..3604)
                     /locus_tag="JMX41_RS27410"
     CDS             complement(3320..3604)
                     /locus_tag="JMX41_RS27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(3622..3819)
                     /locus_tag="JMX41_RS27415"
     CDS             complement(3622..3819)
                     /locus_tag="JMX41_RS27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001335786.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            4031..4327
                     /locus_tag="JMX41_RS27420"
     CDS             4031..4327
                     /locus_tag="JMX41_RS27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            complement(4874..5107)
                     /locus_tag="JMX41_RS27425"
     CDS             complement(4874..5107)
                     /locus_tag="JMX41_RS27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1765..1852)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1863..2419
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5..1110)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.748"
                     /coverage="93.0"
                     /evalue="0.0"
     Typing          complement(4771..5207)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.66"
                     /coverage="99.0"
                     /evalue="1.03e-46"
ORIGIN
        1 acgaactcca cgccccatgt cttcgcgttc agtttctgca atgtctcttc ctgcttcctg
       61 atatcttcca ggttcgcctg tatcatcccg ccaagatacc agagcgtccc gcaactcacg
      121 gtaaacagga aaatgaccat ccccagtaac atcatgcccg tattccctgc cagctttaac
      181 acgtccttcc tgtgctgcat catcgcctct ttcacccctt cccggtgctt tttcagtgat
      241 tcctctgtcg aagctgtgaa cagggctata gcgtctttga ttttcgtctc gtttgatgtc
      301 acagccttgc ttacagattt ttcgagcttc ctgaactcgt cgttcagtat tgtctctgta
      361 gattcggctc tctctttcag ctttttctcg aactccgcgc ccgtctgtaa aagattgctc
      421 ataaaatgct cctttcagcc tgatattctt cccaccgttc gggtctgcaa tgctgatact
      481 gcttcgcgtc accctgacca cttcaagccc tgcctctgtg agcgcctgaa tcacatcctg
      541 acggcctttt atctccccga catgataaag agcgtctatc ccgcgtgtga cgctctctgc
      601 aagcgcctgt ttcgtttccg gcaggttatc agagagagtc agtatccggc ggttctccgg
      661 ggcgttgggg tcatgcagcc cgtaatggtg attcaccagc gtctgccagg catcaattct
      721 cggcctgtct gcccggtcgt agtacggctg gaggcgtttt ccggtctgta gctccatgtt
      781 cggaatgaca aaattcagct caagccgtcc cttgtcctgg tgctccaccc acaggatgct
      841 gtactgattt ttttcgagac cgggcatcag tacacgctca aagctcgcca tcaccttttc
      901 acgtcctccc ggtggcagct ccttctccgc gaacgacagc acaccggacg tgtatttctt
      961 cgcaaatggc gtggcatcga tgagttcccg tacttcttcc ggattaccct gaagcaccgt
     1021 tgccccttcc cggttacgct cccttcccag caggtaatca accggaccac tgccaccacc
     1081 ttttccccgg gcatgaaact taactatcat cccgcgtccc ctgttccctg acggccagac
     1141 gcaaccggcg cagctcatcc ccgatggcca tcagtgcggc caccacctga acccggtcgc
     1201 cggaagacca ctgtccgctg ttcaccttac gggctgtctg gttcaggtta ttcccgatgg
     1261 ctgccagctg acgcagtaac ggcggtgcca gtgtcggcag cctcccggaa cgggcaaccg
     1321 gctcacccag gcagacccgc cgcatccata ccgccagttg tttaccctca cagcgttcca
     1381 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctcg cgtttcatcg
     1441 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg actaaacagg
     1501 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg ctgctggaga
     1561 agctcaacga actggacgcg gatgaacagg cagatatctg tgaatcgctt cacgaccacg
     1621 ccgatgagct ttaccgcagc tgcctcgcgc gcttcgggga tgacagtgaa aacctctgac
     1681 atatgcagct cccggagaca gtcacagctt gcctgtgagc ggatgccggg agcagacaag
     1741 cccgccaggg cgcgtcaggg tgttttagcg ggtttcgggg cgcagccctg aaccagtcac
     1801 gtagcgatag cggagtgtat actggcttaa ccatgcggca tcagtgcgga ttgtatgaaa
     1861 cgtgcgccat accgggtgtg aaatgccgca cagatgcgta aggagaaaat gcacgtccag
     1921 gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc
     1981 ggtatctact cactcaaaaa cgaaatgctg ttatccacag aatcagggga taacaccgga
     2041 aaaaacatgt gagcaaaaga ccaggaacag gaagaaggcc gcgccgctgg cgtttttcca
     2101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2161 cccgacagga cttaaagata ccaggcgttt ccccccggaa gctccctcgt gcgctctcct
     2221 gttccgacct taccgtttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2281 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2341 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2401 cttgagtcca acccggtaag acacgactta acgccactgg cagcagccat tggtaactaa
     2461 aaagtggatt tagatacgca gaactcttga agttgaagcc ttatagcggc tacactgaaa
     2521 ggacatcttt tggtatctgt gctccactta agccagctac ccggttaggt aatctgccag
     2581 gattagctaa ccttcgaaaa accacctgcc agggtggttt tttcgtttac aggcagcaga
     2641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat ctttttttct ctaaactgca
     2701 atcccatcag ttcaggagag aaagatagta taattgccct ctcttatatg gaaccaccat
     2761 tcaatcacat catggggata atataagtga aaacgttcgc attgtttatt tttacgtgta
     2821 tatgtattta tagattggaa ttttaacaaa ataaaatgga gttaatatgt tcaaatcatc
     2881 agtaaagtca ataacatcga gtactatact gaccttactt ataggatgtt caagtactga
     2941 aacaaacaaa aacattgttt acaaagttgg tggtgacagc atcattctga ataaatattg
     3001 catacttaaa atgtaccctt cccaatcaga aaaaaacaaa ggcgtcataa tagaagtaaa
     3061 cacagatagt ttatgtaaga aaaaactaaa cgaaataact agtaaaaata tagggaaacc
     3121 cctgatttca tattttgatg gaaaagtaat atcccaacct gcaatcattc attctgcact
     3181 aaatccaacc cattattatc aacctgtgaa cgatgaaaca acagtgatgg aaatcataaa
     3241 agcatattca aaataatatt aatagatata atcctgctta aggtatagct taggcaggat
     3301 tatatttatt cagctcttat tataaggtag tgtatacatg aataatctgc cctgtcccac
     3361 tgacactttt aaatttaatt ttatcaacac cagcgccctt gcaagagatc tgataagatg
     3421 ctggaccaat aacgttaact gttcttggcc cattaggggc ataccagata aaagcaataa
     3481 atggaccggg tacattagac aatgtatcaa aatagacagt gtacgcgata tcaccagtat
     3541 gattttcaag ttcaacccaa ggaccaaaaa agaaaggagg agcccaaaca ttttgagaaa
     3601 tcatataatc tcctgattag ttcaatgcga atgttcttcc cattcactct cgcaagaaac
     3661 atctataagc tcaacatcta gcccactaac agaatcagag aaatacttat aaacatcatc
     3721 ccttgttccc ataaggtctg ccgaacatga tagttcacaa ccattcttat tcctaactac
     3781 aaataagaaa cggtgaagca atactttttc ttctttcatt ttatccctcc ctttatttac
     3841 acagaatata ctgtatatat aaatactagt tttatataca gtataaatta cgccgcatta
     3901 tttttcctgt caatgatatg aattttcatt tttgtgatct gaatcaccct tataaaaatc
     3961 aggaagggaa gattcgcagc agaaaaacag caccggataa catcaggaga aaaaacagag
     4021 aggagataac gtgagcaaaa caaaatctgg tcgccaccga ctgagcaaaa cagacaaacg
     4081 cctgctggct gcacttatcg ctgccggata cgaagagcgg acagcccgtg acctcatcca
     4141 gaaacacgtt tacacactga cacaggccga cctgcgccat ctggtcagtg aaatcagtaa
     4201 cggtgtggga cagtcacagg cctacgatgc gatttaccag gcaagacgca ttcgtctcgc
     4261 ccgtaaattc ctgaacggaa aaaaaccgga agaagggctg aacgcctgga agggtgattt
     4321 accataatca cttattgtac gcaccgccga aacgcgttcg gtgcgatcac ggcagcagac
     4381 aggtaaaaat ggcaacaaac cacccggaaa actgccgcga tcgcacccgg taaattttaa
     4441 ccacatgcat acctatgcag ccaggtgaat cacgcaggaa ttgccagtca gagaccgctg
     4501 aaacggattt ttttcatact ttcggaagcg aagaagacgg ggatttgagc aggaaaacag
     4561 aagttcaggg gaatgagcgc gatctggcaa tagaggcaaa aacacgacta tgaaagacac
     4621 accagaatcg cgcccgtatg cgttttaacg cgttcctgtg ttttcagcaa ggtttatgac
     4681 tgaagcgcgt tcccggtgcc ccgaaccaac cattatccca aaaaatgcac cggaaacgcg
     4741 ctgacaacat tttagcctgt ctctgattac catcccagcg aagggccatc cagtgtccgt
     4801 tcctgtattt ccagctggcg ctcacgctcc agggataact catgttcgcg ttcctgtatc
     4861 agccgttcgt cccttatctc ctgttctcgc tgtataactg gctcaagcgt tctgtccgct
     4921 cgctcaagtg ctgcacctgc tgactcaact tcatgacccg ctcgttcagc atcgcgttgt
     4981 cccgttgcgt aagcgaaaac attttctgca attccacgaa ggcgctctcc cattcgttca
     5041 gccgctgcat atagtcctgt tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct
     5101 ccgtcactct gtgtcactcc atcagttgta cccagtcctt cccctgatgg ggaatcacct
     5161 ctgctgattt cccgtacgga aggaccagaa atttcctgtt cccgtcctgt
//
