LOCUS       NZ_LR890725             3223 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae isolate KSB1_1H plasmid 4, complete sequence.
ACCESSION   NZ_LR890725
VERSION     NZ_LR890725.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06112200
            Assembly: GCF_904866485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:11:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,310
            Genes (coding)                    :: 5,169
            CDSs (with protein)               :: 5,169
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 59 of 141
            Pseudo Genes (incomplete)         :: 86 of 141
            Pseudo Genes (internal stop)      :: 24 of 141
            Pseudo Genes (multiple problems)  :: 25 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890725.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KSB1_1H"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8457 S 144.9821 E"
                     /collection_date="2014"
                     /collected_by="University of Melbourne"
     gene            join(2902..3223,1..2)
                     /locus_tag="JMW72_RS26970"
     CDS             join(2902..3223,1..2)
                     /locus_tag="JMW72_RS26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            680..1165
                     /locus_tag="JMW72_RS26980"
     CDS             680..1165
                     /locus_tag="JMW72_RS26980"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1169..1384
                     /locus_tag="JMW72_RS26985"
     CDS             1169..1384
                     /locus_tag="JMW72_RS26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1552..1884
                     /locus_tag="JMW72_RS26990"
     CDS             1552..1884
                     /locus_tag="JMW72_RS26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             1553..1884
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2615..2806
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(2152..2632)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          2..1482
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.734"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2903..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="8.510000000000001e-64"
ORIGIN
        1 aagtttcatc ctcgggggcg cggcggcggc ggcgggccgg tcgattatct gctgggtaaa
       61 gatcgccagc gcgacggcgc cagcgttctg caggggaagc cggatgaagt ccgggagctt
      121 atcgacgcct cgccctacgc caaaaagtac acttccggcg ttctgtcttt tgccgaacag
      181 gatttaccgc ccggccagcg cgaaaagctg atggcgagtt tcgagcgggt tctgatgccc
      241 ggactcgata aagaccagta cagcgtgctg tgggttgagc accgggacaa ggggcggctg
      301 gagctgaact tcctgatccc gaacacggag cttctgaccg gcaagcggct acagccgtat
      361 tacgaccgcg ccgaccgtcc gcgcatcgat gcctggcaga ccatagtgaa cggcaggctg
      421 gggctgcatg acccgaacgc gccggagaac cggcgggtgc tggtctcgcc ttccgcgctg
      481 ccggaagcga agcaggaagc cgcccaggcg attacgagcg gcctgcttgc ccttgcctca
      541 tccggggagc tgaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc aggttttgag
      601 gtggtgcgca ccacaaaaag cagcatcagc attgccgacc cggacggggg gcgaaacatc
      661 cgactgaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact tagagcagaa
      721 atcgaaagcg cagcagcaga ctaccggcga gatgctgaaa gccgcattca gcgagcacga
      781 gaagtctgtc agagcggaac tgagcgaaag cgagaagaga atcagcgccg ccatcctcga
      841 ccacgaccgg aagctgtcct cagccatgag ccagcgcacg aaagggatgc tgcgcatggt
      901 cagccagacg tggctgacca tcgttctggt ctccgcgctg ctgatagcgt cgagcgcggg
      961 cattctgtgg tggcaggggc agcagatact cgagaactat acgaccatcc gggagcagaa
     1021 gagcacgcag gccatgctgt cagagaggaa cagcggcgta cagctctcga cctgcggcga
     1081 gcagagacgc cgctgcgtga gggtgaaccc ggaagcggga cagttcggag aggactcgag
     1141 ctggatgata ctggcgggga aatagcacat gacggagctg gaaaaacagt tgctgagcgc
     1201 attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga acgccttcgc
     1261 ggaatggcgg acgatgtctg gtcttattca acgggagaac gcggcgctga acgagcgcgt
     1321 cacggtcttg agcaggcagg tgcagagttt gagcgagcag ctgcgccggt tgtcgaaagg
     1381 ctgaacgcga tagaaactca ccggcagcag gagcgggcgg tgcagcacca gaaagcgctg
     1441 gagctggaac gatcgcagtg gcagcatcat ggtccttcgc tatgataagc cgggttctat
     1501 taattagttg ttatgtaaag atatttaaat ttttcaacat gaggtatttt tatgaataca
     1561 tatattttcc tgggaggggc tattgtggct gaagtcatag gcaccacctt tatgaaatat
     1621 tcagaaggat ttacacgcct gtggccgtct gtaggaactg caataagtta ttgcgctgcc
     1681 ttttatctgc tttcccaaac attggaaaat atacctaccg gtattgctta cgcaatttgg
     1741 tctggtgcgg gaattgtact aataagccta gctggttggt tggttagcgg tcaaaaactt
     1801 gattggcccg caattgcagg aatgttcctt atttgcgttg gagttttggt tattaatatt
     1861 ttttcaaaat cttctgcgca ttaacttttt accgaagcat attggccaaa gcaaaaggat
     1921 ttccttgaga tcctttcttt ctgcgcgtaa tctttttccc tgtaaacaaa aaaaccaccg
     1981 ctaccaacgg tggttcgttt gccggaattc agagctacca actctttgtt ccgatggtaa
     2041 ctggctttca cggagcgcag atacctaaac tgtcctttca gtgtagccgc actcaggctt
     2101 tcacttcaag aactctgtag gcattctcta caatctctgc tccgtgaacc cagttaccaa
     2161 tggctaccgc cactggcgat ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac
     2221 cggaacgggc gtggtagtcg gactgaacgg ggggttcgtg catacagtcc atcctggagc
     2281 gaactgccta cccggaaccg agtttcaggc gtggaatgag aaaccgcggc cataacagcg
     2341 gaatgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag ccaccagggg
     2401 gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cgctgatttg agcgtcattt
     2461 tcgtgatact cgtcaggggg gcggagccta tggaaaaacg gcaactccgc atccttttct
     2521 tggcctttcc tgcttcgagc ggcatttgct tatcccgtaa tctgtgagct ggtaacgccc
     2581 gccgcagtct cacggcagga gcgtagcgac cgagtgagcg aggaggcgtc atttcatcac
     2641 cacctgcatt ttgcacttac gcccggcagc cgcatttttc ccctgtcaca tgaagcactt
     2701 tcataaattg ccactccgtc ataacatgtt tagccagtat acactccgct agcgctacgt
     2761 gactggttca gggctgcgcc ccgaaacccg ctaaaaccac tgccgcgcct gcggcttgtc
     2821 caacaccgtg ccgaccggga aaagatttcc cgtccgtccc ggcgttatca ggaggccgga
     2881 ttggcagaca aacgcagcaa aatgctcacc atgtgggtga ctgaggacga gcaccggcgt
     2941 ctgctggagc gctgcgaggg taagcaactc gcggcctgga tgcggcagac gtgcctggac
     3001 gagaagcccg cacgcgccgg caaacttccg tcgatctcgc cggcgctgct tcgtcagctt
     3061 gccggcatgg ggaacaacct taaccagatt gcccgccagg ttaacgccgg tggtggaagc
     3121 ggacacgacc gcgtgcagat tgtcgccgcg ctgatggcca tcgatgccgg actcgagcgg
     3181 ttgcggcatg ccgtactgga aaagggtgct gatgatgatc gtt
//
