LOCUS       NZ_LR890696             4064 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_4E isolate MSB1_4E plasmid 4, complete
            sequence.
ACCESSION   NZ_LR890696
VERSION     NZ_LR890696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357417
            Assembly: GCF_904866455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 11:15:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,793
            CDSs (with protein)               :: 4,793
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 76 of 205
            Pseudo Genes (incomplete)         :: 150 of 205
            Pseudo Genes (internal stop)      :: 34 of 205
            Pseudo Genes (multiple problems)  :: 49 of 205
            Pseudo Genes (short protein)      :: 3 of 205
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_4E"
                     /isolate="MSB1_4E"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2014-02"
     gene            join(3885..4064,1..513)
                     /locus_tag="JMW23_RS25265"
     CDS             join(3885..4064,1..513)
                     /locus_tag="JMW23_RS25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044326273.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRCTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(761..2920)
                     /gene="mobQ"
                     /locus_tag="JMW23_RS25270"
     CDS             complement(761..2920)
                     /gene="mobQ"
                     /locus_tag="JMW23_RS25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_042042005.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRNEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRTERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREVPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3099..3353
                     /gene="mobC"
                     /locus_tag="JMW23_RS25275"
     CDS             3099..3353
                     /gene="mobC"
                     /locus_tag="JMW23_RS25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2563..2921)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.333"
                     /coverage="100.0"
                     /evalue="8.460000000000002e-179"
     Typing          2922..3098
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..513
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.525"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(765..3007)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.93"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggcggaaaa atgccgaggt tcagttacag gaagcgatcg atcgtttatg ggacaggtcg
       61 atcattctca agaatgacga gaagcgtgag gagttccgct ggatccagta tcgggcacag
      121 tacgctaaag gtgaggccaa ggctcgaata accttttctg acgcggtaat gccttatctg
      181 acgcaactga aggggcaatt cacgcgcgtt gttataaaaa acatatcagg tttatcaagt
      241 agttacagta taagaatata cgagttcctc cagcaattcc gctgtacggg agagagaatt
      301 attgctctta atgactttcg ttcaatgtta gggattgaaa ataaatacaa acaattcaga
      361 gacttaaata aaatactaat aaaaccctgt gttgacgaac tgaacaaaaa aagcgacctg
      421 gctgtaacgg ttgagacgat caaaaagggg cgcacggttg tagccctgca ttttcgcttc
      481 aaggaagaca aacagatcaa gatgacgatc taaaatcgat tttaatgcac ctaaaacgct
      541 ccctgctgcg ttttttttgt gttagtagtg cgaaggtact attgaagtga aaaattgcgc
      601 caaaaacgct ctgatgcagc gaaaaaagaa cccccaattt ttgggggcgg aaaaacaaaa
      661 ctatgcttgc gttatgtgag tgtgcttctt gcattcatcc ctgtcgatga acatcgacga
      721 acataacacg atgttaaaca aaacaaaata ttgttcacat tcactgtgac caaccgcgat
      781 catgtctcct ctcggtggtg tgtgttcttg catcttcctc gcgccgccat tcgtcccagg
      841 tacgattgaa acccagcgag cggaacagat gatccggctg tttttctggt gtgttgacgg
      901 cagcaaaaag cggtacctca cgggttttca gatgaaactc cataagttcc ggcgcaggtg
      961 atatgtggct gtctgcatcg tgatttacgg caacaccata ccctgcatac cgtaacccct
     1021 tatcggtcag tgcactgatg accagctggc tgttatcggc tgagggaacg gcaaacgcgg
     1081 gatacagcgt accattaagc cggacaacac cgcccagaga gtcatccacg cccatgacca
     1141 gcaaaagctg aagctggcgg gtttcccggt cgacagaact cattaccggc atcagcgtcg
     1201 gtggcagacg tccctgcgta aaatccacct tccttgctgc cagtgatggc atggctggct
     1261 ggcggtcttc tccaagacag aaagactccg gccatgtctt cggctggcgg agattgcggg
     1321 tatattgcgg ccatgtctgg caaacctgcg tcagaagttg ctcaaaggtc gcagaggaat
     1381 aaacacgttg cagtggtgga tgttgaccga tttcgcttcg gtcacagata taaccactcc
     1441 tgtcacgcat taaatcaaca cgctcaaaga ttctggtgcg gtatttctcc agctcgttcc
     1501 cccagagcgt gtattctccg ttccggttgt gaagcagaag gtaacaggtc atttccccgt
     1561 taccaggcat gggggcctcg ccataccccg taacgactgc gctcagtacc cctgaatcca
     1621 taacggcatt gtcatttttc ctgagctgtt ccgctcgtcg ctccgtgcga ctacgttctg
     1681 tatcctcctg ctccttccgg cgcaattcct cttcacgttt aagcaactgg actctgagag
     1741 ctgcaaggcc ggatgccccc cactgctgcc actgtttttc aaatgccgtc tgactcgcgg
     1801 gaatgtcctg tttctccacc ttccggtaat gctgtcgcaa tttttccatt tgctggctca
     1861 gcgactgaat ttttccctgt tcgttacggt attccggtgc cgcccattgc atcaggggaa
     1921 tacgggaaaa accggacgaa aacggtgatg attttttcag gctggcagca tggctcatcg
     1981 cttcctttcg ttcggtgttc agctcttccc agcgtctgcg cgtcgcctgc atatctgcca
     2041 tcatcgacat ggcattactg gtgagtttcc atgccgcatt actgtccttc ggcaacgctt
     2101 cgatgatgtc acgtagtgtg tacgaagttg cgttgtcagg aagcacgata ttcagaaggt
     2161 cagccacacg gcgggcaggc tctgtcagcc cgctgttcct gagttcagtg cgggaaatct
     2221 gtatttccag gttaagcctg ttaatcagac gattctgttc gccgcgttct gtttcgatac
     2281 cgcgtgtttc catcgcacag gcagactttc cgaggtgaat accaggcgtt ttctcaagcc
     2341 cctgtcgctc gtatgaacga tggtctatct cttcctgata accggcgttc gccagtgctc
     2401 tgtttgcatg gtcagcccat gatgcacgcc acgtctcggc atgtgtccgg tcgttccagt
     2461 ccctgacctt tccgccgaac ccagcaggtc cgacagccct cgtggtcagc atgatgtgcg
     2521 catggggatt tgttttatcc atgtgatgga acgccacatc agcaatcatg ccctgactga
     2581 caaagttttc tctgacaaag gcgagaacgg tttcccgtgc ggcatcctgc ggcagttcac
     2641 gggggatagc gagttcaatt tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct
     2701 caacggcatt ccagagttcg gcacggttcg tacaccaggc aggggctgtc gcgggggtca
     2761 gaatcacagc ttcagccaca ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct
     2821 catcaagcat cagtgaaccg gaacgatagg cagcggaagc tacggcgctg tagccgctgc
     2881 ttctggagat aattttcatg ctgagatgat aaatcgccat ttctgccgta atgccttttt
     2941 gtagttttct ttcagccccg cagggcgcac acaatcgaaa cggagtggag attgtataag
     3001 tgcgcacttc gtgatttgcc gtgcattatg ccagctcagt ttttaaacta ccagtgacgc
     3061 gctaaaatgc gatttttcat cgcgcacgga gttacatcat gagcagaacg ctcgaacaga
     3121 agattgctga tgctgaggcg agattacagc gcctgaaggc gaagagccgg agcttggaca
     3181 cagcgcagaa ggttgttgtg ggtgcagcat tgctggcaaa ggtcagaaag ccggaggagg
     3241 tgcagttgcg tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg
     3301 tgacgcgcat actgccgctg attaacgagc tggaggcgct gccaggacag tgattgtatc
     3361 gtccgctcgc cgcagcccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa
     3421 gtgaatccgt cgccaggttg agcacatacg cacggttttt cggcaggtga tatgcgctga
     3481 acccgcatga atactggatt tccacagggt tggaagggga gtattcggaa cttgcaattc
     3541 tactttcttg caacacattg tttttatttg ttttttgttt tcagcacccc aagttatcca
     3601 caggctcgcc gttaagtttt tagatcttgt ttttaagatc ttgtttttaa tacttgtttt
     3661 aggggcgaaa ttcattaata aaatcaatat gttgcagtgg tatatgtgga ggaatttaag
     3721 gtatatgtgg aggaatttaa ggtgtttagg tggaggaatt taaggtaaag gtggaggaat
     3781 ttaaggtgtt attcacagaa atgtgaggat ataaaaaaaa atattccaca tttcttattt
     3841 gtggggcaca ttaaagcatc accacatttg aacgggatct ttgtatgtcg gacttgattg
     3901 cctacaagtc gaatgctttg gttgaggcca gctacaagtt gaccctacag gaacaacgtt
     3961 ttctgttgct gtgtatcagt cgattgaaat ctggtgctga tgctgaattg caaaagacaa
     4021 tgaccattac cgctgcggaa tattttgatt cattccctga catg
//
