LOCUS       NZ_LR890655             4618 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_2E isolate MINF_2E plasmid 5, complete
            sequence.
ACCESSION   NZ_LR890655
VERSION     NZ_LR890655.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357530
            Assembly: GCF_904866305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:18:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,083
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 1 of 210
            Pseudo Genes (frameshifted)       :: 93 of 210
            Pseudo Genes (incomplete)         :: 126 of 210
            Pseudo Genes (internal stop)      :: 42 of 210
            Pseudo Genes (multiple problems)  :: 45 of 210
            Pseudo Genes (short protein)      :: 2 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890655.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_2E"
                     /isolate="MINF_2E"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="5"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            complement(30..263)
                     /locus_tag="JMW95_RS25725"
     CDS             complement(30..263)
                     /locus_tag="JMW95_RS25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(270..788)
                     /locus_tag="JMW95_RS25730"
     CDS             complement(270..788)
                     /locus_tag="JMW95_RS25730"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_072650569.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTVSG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKG"
                     /name="MbeB family mobilization protein"
     gene            complement(<826..1257)
                     /locus_tag="JMW95_RS26185"
                     /pseudo=""
     CDS             complement(<826..1257)
                     /locus_tag="JMW95_RS26185"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1466..1789)
                     /locus_tag="JMW95_RS25740"
     CDS             complement(1466..1789)
                     /locus_tag="JMW95_RS25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_201618526.1"
                     /translation="MLTIRVTDDEHAGLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1853..2044
                     /locus_tag="JMW95_RS25745"
     CDS             1853..2044
                     /locus_tag="JMW95_RS25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2704..>2784
                     /locus_tag="JMW95_RS26190"
                     /pseudo=""
     CDS             2704..>2784
                     /locus_tag="JMW95_RS26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3280..3927
                     /locus_tag="JMW95_RS25750"
     CDS             3280..3927
                     /locus_tag="JMW95_RS25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_001399740.1"
                     /translation="MKTIVYVDGFNFYYRKLKDSNKKWLNIKAMVEKLLPDDEIIGINY
                     YTARISGRIDSDAPRRQQIYFNALKSIDNLSIHYGTFSVNESRMFLVQPLDFKHHLKNP
                     NQKIKFANVLKTEEKGSDVNLGCHLVRDGFQGKYEKAVIITNDTDLAEPMRVVKSELSL
                     YIELLSPVNKPARTLSAICDKVTLISDQSIADSQFEDVIVVGEKTVTKPAQW"
                     /name="NYN domain-containing protein"
     gene            complement(4048..4284)
                     /locus_tag="JMW95_RS25755"
     CDS             complement(4048..4284)
                     /locus_tag="JMW95_RS25755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125075503.1"
                     /translation="MSIIIDREKYTALMTLIQTSQGYVGSKVQPNGYTLKCSFDTGFAC
                     CISRNTRNSNLSVHFENESTDPATHFAILSAIS"
                     /name="hypothetical protein"
     Typing          complement(2126..2207)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          2227..2773
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.74"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(59..1476)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.829"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgcgctcc tgtatcaacc gttcctctct tatctccagt tctcgctgta taactggctc
       61 aagcgttctg tctgctcgct caagtgctgc acctgctgac tcaactgcat gacccgctcg
      121 ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacatct tctgcaattc cacgaaggcg
      181 ctctcccatt cgctcagccg ctgcatatag tcctgttgca gctgctctaa ggcgttcagc
      241 aaatgtgttt ccagctctgt cactctgtgt caccccttca gatgtaccca ttccttcccc
      301 tgaaaggaaa tcacctctgc tgacttcccg taaggaagga ccagaaattt cctgttcccg
      361 tcctgcacaa actccacgcc ccatgtcttc gcgttcagtt tctgcaatgt ctcttcctgc
      421 ttcctgatat cttccaggtt cgcctgtatc ctcccgccaa gataccagag cgtcccgcca
      481 ctcacggtaa acaggagaaa gactatcccc agcaacatca tgcccgtatt ccctgccagc
      541 tttaacacgt ccctcctgtg ctgcatcatc gcctctttca ccccttcccg gtgtttttcc
      601 agagattcct ctgtcgaggc tgtgaacagg gctatagcgt ctctgatttt cgtctcgttt
      661 gatgtcacag cctcgcttac agattcgccg agcctcctga actcgttgtt cagcattttc
      721 tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt ctgcaaaaga
      781 ttgctcataa aatgctcctt tcagcctgat attcttccca ccgttcgggt ctgcaatgct
      841 gatactgctt cgcgtcaccc tgaccacttc aagccctgcc tctgtgagcg cctgaatcac
      901 atcctgacgg ccttttatct ccccgacatg ataaagagcg tctatcccgc gtgtgacgct
      961 ctctgcaagc gcctgtttcg tttccggcag gttatcaggg agagtcagta tccggcggtt
     1021 ctccggggcg ttggggtcat gcagcccgta atggtgattc accagcgtct gccaggcatc
     1081 aattctcggc ctgtctgcgc ggtcgtagta cggctggagg cgttttccgc tctgtagctc
     1141 catgttcgga atgacaaaat taagctcaag ccgccccttg tcctggtgct ccacccacag
     1201 aatgctgtac tgattttttt cgagaccggg catcagtaca cgctcaaaac tcgccatcac
     1261 tttttcacgt cctcccggcg gcagttcctt ctccgcgaac gacagaacac cggacgtgta
     1321 tttcttcgca aatggcgtgg catcgatgag ttcccggact tcttccgggt taccctgaag
     1381 cacctttgcg ccctcccggt tacgctccct ccccagcagg taatcaaccg gaccactgcc
     1441 accgcctttt cccctggcat gaaacttaac tatcatcccg cgtcccctgt tccctgacgg
     1501 ccagacgcag ccggcgcagc tcatccccga tggccatcag tgcggccact acctgaaccc
     1561 ggtcaccgga agaccactgc ccgctgttca ccttacgggc tgtctgattc aggttatttc
     1621 cgatggcggc cagctgacgc agtaacggcg gtgccagtgt cggcagtttc ccggaacggg
     1681 caaccggctc acccaggcag acccgccgca tccataccgc cagttgttta ccctcacagc
     1741 gttcaagtaa cccggcatgt tcatcatcag taacccgtat cgtgagcatc ctctcgcgtt
     1801 tcatcggtat cattacccca tgaacagaaa tcccccttac acggaggcat cagtgactaa
     1861 acaggaaaaa acagccctta acatggcgcg ctttatcaga agccagacat taacgctgct
     1921 ggaaaagctc aacgaactgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     1981 ccacgccgat gagctttacc gcagctgcct cgcacgtttc ggggatgacg gtgaaaacct
     2041 ctgacacata cagctcccgg agacggtcac agcttgtctg tgagcggatg ccgggagcag
     2101 acaagcccgt cagggcgcgt cagggggtgt cggggcgcag ccatgaccca gtcacgtagc
     2161 gatagcggag tgtatactgg cttaactatg tggcatcagt gcagattata agaaaagagc
     2221 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaatgccgc atctggcgct
     2281 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcggctgcgg cgagcggtat
     2341 aagctcacgc aaagacggta atgcggttat ccacagaatc aggggataag gccggaaaga
     2401 acatgtgaac aaaaaggcaa aaccaaagga agaggccgcg ccgcaggcgt ttttccatag
     2461 gctccgcccc cctgacgagc atcacaaaaa ttgacgctca agtcagaggt ggcgaaaccc
     2521 gacaggactt aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2581 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2641 tctcatagct cacgctgttg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc
     2701 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgtct tatccggtaa ctatcaactt
     2761 gagtccaacc cggaaagaca cgactaatcg ccattggcaa cggccactgg taaacaggat
     2821 gtgcgagaga tgtatcgaga gttcttgaag tggcgacctg actacggcta cactgaaagg
     2881 acagatttgg tgacatgtct cgcacaagac cgttaccgcg gttagagaat ctgccaggat
     2941 taactaacct tcgaaaaacc acctgccagg gtggtttttt cgttttcaaa gcaggagatt
     3001 acgcgcagaa aaaaaggatc tcaagaagat cttttctcct ttaggtaggg cttacttcgt
     3061 caccatataa aacatttgca ctagacaaaa aaaatgcaat aatgttggta cagacctgtg
     3121 gcgtgccgca ggcgaagaaa caacccccct gtggcgtgcc gcaggcgaag aaacaaccct
     3181 cctgtggcga gccgcaggca aacacccctc ctacggcgtg ccgtaggaaa aaaaaccgca
     3241 gatgcggttt tttttatatc taaaatggag ctaaccccat tgaaaacaat agtttatgtt
     3301 gatggcttta acttttatta tcgtaagctg aaagattcca acaaaaagtg gctgaacata
     3361 aaagcaatgg tcgaaaaatt gctaccggat gatgaaataa taggaattaa ctattacacg
     3421 gctaggatct caggaaggat tgattctgac gcacctcgta ggcaacaaat atatttcaat
     3481 gcactgaaaa gcatagataa cctaagtatc cattatggta ctttttcggt gaatgaaagc
     3541 agaatgtttt tagtgcagcc tcttgatttc aaacatcact taaaaaaccc gaatcaaaaa
     3601 ataaagtttg caaacgtctt aaagacagaa gaaaagggtt ctgacgttaa tttaggttgc
     3661 cacttggtta gagatggttt ccaagggaaa tacgagaagg ctgtaataat aactaatgat
     3721 acagatttgg ctgaaccaat gagagtagtc aaatcagagc tcagccttta tattgaacta
     3781 ctttctcctg taaacaaacc agcaagaacc ctgagcgcaa tatgcgataa ggtcactctt
     3841 ataagcgacc aatcaatagc tgatagccag tttgaagacg ttattgtagt tggtgagaaa
     3901 accgtgacaa aaccagcaca gtggtagtgg ctacatactc gttctgttga aaaaaagccc
     3961 atcccccggg ctttttatgt tttgcattat accttcaggc aaatataaag agtatgaaat
     4021 ctatagctat gtgcagctaa taaaaaatta gctaatagca cttaaaatag caaagtgagt
     4081 tgcaggatct gtactttcat tttcaaaatg aacagataag ttactatttc tagtatttct
     4141 agagatacaa catgcaaaac cagtgtcgaa agaacacttc aatgtatatc catttggttg
     4201 tacttttgag ccaacatatc cttgagatgt ctggatcaac gtcatcaaag cagtatattt
     4261 ttcacgatca ataataatac tcatcacatc cccattagta ttactctgca ggcattaaac
     4321 ctcagcaata aatctattta ccataactcc cgttgcccgc accgcctcag acgctctcag
     4381 cgcgtttctg acgggaaacc ggaaaaacag gcaacaaacc tcccgaaaaa ctgccgcgat
     4441 cgcgcctgat aaattttaac cgcatgaata cctatgcaac aagatggtgc agactacatt
     4501 agtccgtcaa aatccactga acctaccatt tttcatagtt tcaccatccc agcgaagggc
     4561 catccagcgt gcgttcctgt atttccggct gacgctcccg ttccagggat aacgcata
//
