LOCUS       NZ_LR890654             6062 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_2E isolate MINF_2E plasmid 4, complete
            sequence.
ACCESSION   NZ_LR890654
VERSION     NZ_LR890654.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357530
            Assembly: GCF_904866305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:18:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,083
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 1 of 210
            Pseudo Genes (frameshifted)       :: 93 of 210
            Pseudo Genes (incomplete)         :: 126 of 210
            Pseudo Genes (internal stop)      :: 42 of 210
            Pseudo Genes (multiple problems)  :: 45 of 210
            Pseudo Genes (short protein)      :: 2 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890654.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_2E"
                     /isolate="MINF_2E"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            complement(30..263)
                     /locus_tag="JMW95_RS25685"
     CDS             complement(30..263)
                     /locus_tag="JMW95_RS25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(270..788)
                     /locus_tag="JMW95_RS25690"
     CDS             complement(270..788)
                     /locus_tag="JMW95_RS25690"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_201618524.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTVSG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKG"
                     /name="MbeB family mobilization protein"
     gene            complement(<826..1257)
                     /locus_tag="JMW95_RS26180"
                     /pseudo=""
     CDS             complement(<826..1257)
                     /locus_tag="JMW95_RS26180"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1466..1789)
                     /locus_tag="JMW95_RS25700"
     CDS             complement(1466..1789)
                     /locus_tag="JMW95_RS25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956001.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1854..2045
                     /locus_tag="JMW95_RS25705"
     CDS             1854..2045
                     /locus_tag="JMW95_RS25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2697..2810
                     /locus_tag="JMW95_RS25710"
     CDS             2697..2810
                     /locus_tag="JMW95_RS25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072101919.1"
                     /translation="MHEPPVQSDRCAFSGNYQLESNPERHDKTPMAAATGN"
                     /name="replication initiation protein"
     gene            complement(3045..3482)
                     /locus_tag="JMW95_RS25715"
     CDS             complement(3045..3482)
                     /locus_tag="JMW95_RS25715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052918195.1"
                     /translation="MPKRGKSIRSSSRNEETKPSKSTAKTTDRFISISFKHFHNVDDVG
                     QSINTWKDDGSLPDLIEKLVYLTANGITKVISDGVFTNYTRFPESAVNDFRCPDEISSD
                     EDWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSQK"
                     /name="hypothetical protein"
     gene            complement(3487..4785)
                     /locus_tag="JMW95_RS25720"
     CDS             complement(3487..4785)
                     /locus_tag="JMW95_RS25720"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_052918197.1"
                     /translation="MMVNFSLSNAFSFMEAQNMSMLAVSSSKDDINLANVVSVRDERIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVVPFLLDEAGPTKPSEME
                     MTFIADGIKYRYGLSLFQGKIKEEWLYYTPKTRETILFERDGQLIEINKAAFSESNLFI
                     KDGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFSNVVFLSGSNEGMFTGITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPEFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTANGEISVPIDFESEGTKKIVYLLGPIYNAMINNNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGFSQVIAAVQDVNLMNTDCFRRDQIWFVDKDVKSGSSHLYSLVEYKEKQRS
                     LKPSYGNDYLSGAFDAIPLFDNLNEFEKLMPEG"
                     /name="ATP-binding protein"
     Typing          complement(2131..2218)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2229..2766
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.814"
                     /coverage="99.0"
                     /evalue="3.760000000000001e-179"
     Typing          complement(59..1476)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.56"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgcgctcc tgtatcaacc gttcctctct tatctccagt tctcgctgta taactggctc
       61 aagcgttctg tctgctcgct caagtgctgc acctgctgac tcaactgcat gacccgctcg
      121 ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacatct tctgcaattc cacgaaggcg
      181 ctctcccatt cgctcagccg ctgcatatag tcctgttgca gctgctctaa ggcgttcagc
      241 aaatgtgttt ccagctctgt cactctgtgt caccccttca gatgtaccca ttccttcccc
      301 tgaaaggaaa tcacctctgc tgacttcccg taaggaagga ccagaaattt cctgttcccg
      361 tcctgcacaa actccacgcc ccatgtcttc gcgttcagtt tctgcaatgt ctcttcctgc
      421 ttcctgatat cttccaggtt cgcctgtatc ctcccgccaa gataccagag cgtcccgcca
      481 ctcacggtaa acaggagaaa gactatcccc agcaacatca tgcccgtatt ccctgccagc
      541 tttaacacgt ccctcctgtg ctgcatcatc gcctctttca ccccttcccg gtgtttttcc
      601 agagattcct ctgtcgaggc tgtgaacagg gctatagcgt ctctgatttt cgtctcgttt
      661 gatgtcacag cctcgcttac agattcgccg agcctcctga actcgttgtt cagcattttc
      721 tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt ccgtaaaaga
      781 ttgctcataa aacgctcctt tcagtctgat attcctcccg ccgttcgggt ctgcaatgct
      841 gatactgctt cgcgtcaccc tgaccacttc aagtcccacc tctgtgagcg cctgaatcac
      901 atcctgacgg ccttttatct ctccggcacg gtaaagcaca tcaagccccc gtgtaacgct
      961 ctcagcaagc gcctgtttct tttccggcag gttatcaggg agagtcagcg tccggcggtt
     1021 ctccggggcg ttggggtcat gcagcccgta atggtgattc accagcgtct gccaggcatc
     1081 aattctcggc ctgtctgcac ggtcgtagta cggctggagg cgttttccgg tctgtagctc
     1141 catgttcgga atgacaaaat tcagctcaag ccgtcccttg tcctggtgct ccacccacag
     1201 gatgctgtac tgattctttt cgagaccggg catcagtaca cgctcaaaac tcgccatcac
     1261 tttttcacgc cctcccggcg gcagctcctt ctccgcgaac gacagaacac ctgacgtgta
     1321 tttcttcgca aatggcgtgg catcgatgag ttcccggact tcttccggat caccctgaag
     1381 caccgttgcc ccttcccggt tacgctccct tcccagcagg taatcaaccg gaccactgcc
     1441 accacctttt cccctggcat gaaacttaac tatcatcccg cactccctgt tccctgacgg
     1501 ccagacgcag ccggcgcagc tcatccccga tggccatcag tgcggccacc acctgaaccc
     1561 ggtcaccgga agaccactgc ccgctgttca ccttacgggc tgtctgattc aggttatttc
     1621 cgatggcggc cagctgacgc agtaacggcg gtgccagtgt cggcagcttc ccggaacggg
     1681 caaccggctc acccaggcag acccgccgca tccataccgc cagttgttta ccctcacagc
     1741 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctcgcgtt
     1801 tcatcggtat cattactccc gtgaacagaa atccccctta cacggaggca tcagtgacta
     1861 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgctgc
     1921 tggagaagct caacgaactg gacgcagatg aacaggcaga tatctgtgaa tcgcttcacg
     1981 accacgccga tgagctttac cgcagctgcc tcgcacgttt cggggatgac ggtgaaaacc
     2041 tctgacacat gcagctcccg gagacggtca cagcttgtct gtgagcggat gccgggagca
     2101 gacaagcccg tcagggcgcg tcagcaggtt ttagcgggtg tcggggcgca gctctgaccc
     2161 agtcacgtag cgatagcgga gtgtatactg gcttaaccat gcggcatcag tgcggattgt
     2221 atgaaaagta cgccatgccg ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcat
     2281 gtcccggcgc ttttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     2341 gcgagcggta ctgactcaca caaaaacggt aacacagtta tccacagaat caggggataa
     2401 ggccggaaag aacatgtgag caaaagacca ggaacaggag gaaggccgcg ccgctggcgt
     2461 ttttccatag gctccgcccc cctgacgagc atcacaaaaa ccgacgctca agtcagaggt
     2521 ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtgact ccctcgtgcg
     2581 ctctcctgtt cctgccattc ggtttaccgg tgtcactccg ctgttatggc cgcgtttgtc
     2641 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     2701 acgaaccccc cgttcagtcc gaccgctgcg ccttttccgg taactatcaa cttgagtcca
     2761 acccggaaag acacgacaaa acgccaatgg cagcagccac tggtaactga tttagaggag
     2821 ttagtcttga agttatgcgc ctgttaaggc taaactgaaa gaacagattt ggttactgcg
     2881 ctcctcataa gccagttacc ttggttcaca gagcgccaac tcttagaacc gtcgaaaaac
     2941 cgcccgccaa ggcggttttt tcgtttacag agcaagagat tacgacgaga gcatcacaat
     3001 ctcaagaaga tcttttctta acatacaata caactgtatc tcatctattt ttgactcttc
     3061 cagaagcgat gatctctatc aaaaaacacg atatagaaca cattatctct cagaaatccg
     3121 gcgactcttc ttttctgccc gccaatgttt tttataaccc cccaatcctc atcagaggat
     3181 atctcatctg ggcatctaaa atcgtttaca gctgactcag gaaatcgagt gtagttagta
     3241 aacacaccgt cagaaatcac tttagtgatt ccattcgccg ttagataaac caatttttca
     3301 atgagatcag gtaggctacc atcatctttc catgtattta tggactgccc aacatcatcg
     3361 acgttatgaa aatgcttaaa actaatagat ataaatctat cagtcgtttt cgctgtgctc
     3421 tttgaaggtt ttgtttcctc attcctcgaa gagcttcgaa tgcttttgcc tctctttggc
     3481 atataatcat ccctcaggca ttagcttttc aaattcatta agattatcaa ataacgggat
     3541 tgcatcaaaa gcacctgata aataatcatt cccatatgat ggcttcaatg atctttgttt
     3601 ctctttgtac tcaaccaaag aataaagatg gctactccct gactttacat ccttgtcaac
     3661 aaaccaaatt tggtctcgtc taaagcaatc agtattcatt aaattaacat cctgaactgc
     3721 agcgattact tggctaaacc cttcactttg cgcatgaaat atcctaaata aaaacttaga
     3781 taaaagggta tggaactttg aatcgaactc atcaaccaat aataagttat tgttaatcat
     3841 ggcgttataa ataggcccaa ggagatagac tatctttttg gtcccctctg attcaaaatc
     3901 aatgggaacc gaaatctccc cattagcagt ttttttcacg actctaagag aaaatacttt
     3961 ttgatttttt gcaaaattct gaatcgagct gaataaattt tttaaattat catcatctgt
     4021 ttttatgttg ccaaacggaa attcaggcgc aacttcatca agataaatat cattaatctg
     4081 aaaggctggt aatatttttt ttagccaagc tttaaaatct tcagattcat ttataagctt
     4141 catggtaatt ccagtaaaca ttccttcgtt agaaccagaa agaaatacca cattactaaa
     4201 ccaattcaca acacgctttg agtgctcccc attaaatcca gcaacaacag aaacaaaggg
     4261 aacatcttct cttgtcttct gaatgacccc atcctttata aaaagatttg actcgctaaa
     4321 tgctgcttta tttatttcaa taagttgacc atctcgctca aagagaatag tctcccttgt
     4381 ttttggcgtg tagtacaacc actcttcttt tatcttccct tgaaataaag ataatccgta
     4441 tctgtattta atcccatccg caataaaagt catttccatt tcagatggtt tagtaggccc
     4501 cgcctcatca agtaaaaaag ggactacatt cttcgtaatg ctactttcga tagcttgcac
     4561 agaatgcagg acaatatctt tcattaaaga tattgtcttc atgagattag attttccgct
     4621 agcgttagct ccgaaaatca aagcagactt cagtatccgc tcatccctta ctgacactac
     4681 attagccaag tttatatcgt ctttcgatga cgacaccgcc aacatgctca tattttgagc
     4741 ttccatgaag gaaaatgcgt ttgaaagact aaagttaacc atcataagaa cacccctaac
     4801 aacaccctta aactcaaaaa ttgcacaata ttgacaaaat tctagattaa gaatagcata
     4861 accccgtact aaaacaacaa aagggtccaa tagacagcac attatgagta gataagttaa
     4921 cgaccagctt aaaaagaggt gtatatgaag ttttttctat accaaatggt cccatttgcc
     4981 tcagtgatta gctcagcagt gctggctttt cactcggtat ctgggtgggg atggttcctt
     5041 tttgtcgcgt ttatgctcag tggcgctgac actgtaaaaa aatacgtagg gagtgaaaca
     5101 tgaatgagga gaagcaaaaa ttgttgaagt aagtgggtta gcctgtacca ccagaactaa
     5161 cccgtaacaa accatacgca aatgcggcgc ttgagtacca cctcaagtgc tttgataaag
     5221 ccagccacaa gatctgttgg ttgctacagt acaattttga tagcaaaaat caacattcat
     5281 gtggcgaaaa taggaaatgc aacatgcaga cggacgagcc tcaggcatac atttttcgcc
     5341 cttacataac ggtaaagggc aaacggataa cccgtcctaa cgggggaatg tttaagatcc
     5401 caattaatcg ggaaaagaaa aaacaataac caccgaagca tgcaagcttc ggtccctaac
     5461 ttagtttatg taaggaaagc aacatggcct tttatgtaga tagcacagcg aaatttttaa
     5521 caaaaacaac cgagcctgaa gggagtgtat ggcggtatta ctataagccg ggaaatactt
     5581 gtccggcttc cggcatttac cgctgcacga actgtggtga tgaagtgacg tgcaataaag
     5641 atgatccttt gccaccgcaa aatcacaggc agcataatgt cccaggtgac atccgatggg
     5701 aattggtagt gatgacaaag acgaagtaac ttactaggcc gtctacggac ggctttttta
     5761 tacctgccaa tcagctggat ttaccataac atccgaattg tacgcaccgc taaaacgcgt
     5821 tcagcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaaatgccg
     5881 cgatcgcgcc tgataaattt taaccgtatg aatacctatg caaccagatg gtacaggcca
     5941 cattcccccc acttaatcca ctgaagctgc catttttcat ggtttcacca tcccagcgaa
     6001 gggccatcca gcgtgcgttc ctgtatttcc ggctgacgct cccgttccag ggataacaca
     6061 tg
//
