LOCUS       NZ_LR890630             3972 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Klebsiella pneumoniae isolate KSB1_6F plasmid 4, complete sequence.
ACCESSION   NZ_LR890630
VERSION     NZ_LR890630.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06112198
            Assembly: GCF_904866355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_904866355.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 18:31:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,257
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 5,029
            CDSs (with protein)               :: 5,029
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 51 of 101
            Pseudo Genes (internal stop)      :: 17 of 101
            Pseudo Genes (multiple problems)  :: 19 of 101
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890630.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KSB1_6F"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8457 S 144.9821 E"
                     /collection_date="2013"
                     /collected_by="University of Melbourne"
     gene            join(3646..3972,1..441)
                     /locus_tag="JMW92_RS26255"
     CDS             join(3646..3972,1..441)
                     /locus_tag="JMW92_RS26255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_130239122.1"
                     /translation="MRFLWRPLPYSRLRECDFLNCIDENDSSSWIAALKIFICIVIKSD
                     LDENGEYCAMITYPQVQKMTGMSRQLICCGFDKLKFYKMISSEGVRKKKYSLLTCEKGS
                     TRQKPLTKRFDGLSTGRWCKFPLKGLIDDDGVIPSFLAMSNRNINDLHALRLFIYLLSV
                     RRHGDVFIVVKAETLMKKLRLKRVQLTLALAHMSALGLIVKERYRDGDFLSLNKTPAYA
                     FLLCGWDVLEWNHNRIKESNWRNDYLESFDNWV"
                     /name="hypothetical protein"
     gene            complement(462..635)
                     /locus_tag="JMW92_RS26260"
     CDS             complement(462..635)
                     /locus_tag="JMW92_RS26260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153848860.1"
                     /translation="MDIKTKLKRSLDSVESAISSLKRARGQTTDAYHEINKAIRELEDA
                     EYNIRKSIREME"
                     /name="hypothetical protein"
     gene            complement(972..1121)
                     /locus_tag="JMW92_RS26265"
     CDS             complement(972..1121)
                     /locus_tag="JMW92_RS26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077253766.1"
                     /translation="MHEPPVQSDCCACSGNYQLESNPERHDKAPLAAATGKQDVREMYL
                     EFLK"
                     /name="replication initiation protein"
     gene            1537..1728
                     /locus_tag="JMW92_RS26270"
     CDS             1537..1728
                     /locus_tag="JMW92_RS26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1728..2150
                     /locus_tag="JMW92_RS26275"
     CDS             1728..2150
                     /locus_tag="JMW92_RS26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_085856034.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRAFGSLDGVIDTVTYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAIQLLEHKHEPSSPPHSSWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3015..3170)
                     /locus_tag="JMW92_RS26280"
     CDS             complement(3015..3170)
                     /locus_tag="JMW92_RS26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="WP_064761103.1"
                     /translation="MNDTWDFYTDGKGEWRWRRTASNGRIVGASSQGYVNRADCIANAR
                     RNGYTG"
                     /name="DUF1508 domain-containing protein"
     Typing          complement(1013..1416)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.309"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttgacgaaaa ggtttgatgg gctatcaaca gggagatggt gtaagttccc gttaaaagga
       61 ttgattgatg atgatggtgt aatcccttct tttctggcta tgagcaatag aaatattaac
      121 gaccttcatg cattgcggtt gtttatctat ttattgtcag tgagacggca tggtgatgtt
      181 tttattgttg taaaagcaga aacgctgatg aaaaaattac gacttaaaag agttcaacta
      241 acattggctt tagctcatat gtcagctttg gggttgatag ttaaagagcg atatagggat
      301 ggcgattttt tgagtttaaa taaaacgcct gcatatgctt ttttattgtg tggatgggat
      361 gttcttgagt ggaatcacaa tagaataaaa gagagtaact ggagaaatga ttatttagag
      421 tcgtttgata actgggtttg aagcccagtt atctatatag ctcactccat ctcacgaatg
      481 gatttcctta tgttgtactc ggcatcctca agttcacgaa ttgctttgtt tatttcatgg
      541 tatgcatccg tagtttgccc tcttgcacgc tttaaagatg aaattgcact ttccacagaa
      601 tctaaagaac gtttaagctt tgtcttgata tccatattga acctctgttt tttatctgct
      661 gctagggtga ttgtggaaca ccctaatgaa tttgttttat ttaaataaaa tcaatatgat
      721 acgtcttttt ttgaagtcta ttggtattgt taagtggtgc tggtttggtt ttgatatatg
      781 tctatgtatc taacggattt gcttgtgatc ctttcctcgg cgtcgtagtc tcttgctctg
      841 taaacgaaaa aaccacccgg caaggtggtt tttcgaaggt tcattaaccg gcaagtttat
      901 gaaccgtggt aacagtcttg tgcgagacgt gtcaccaaat ccgtccttcc agtgtagcct
      961 cagttaggtc ttcacttcaa gaactctaaa tacatctctc gcacatcctg tttaccagtg
     1021 gctgctgcca gtggcgcttt gtcgtgtctt tccgggttgg actcaagttg atagttaccg
     1081 gaacaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     1141 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     1201 aatgacaccg gtaaaccgaa tggcaggaac aggagagcgc acgagggagc catcaggggg
     1261 aaacgcctga tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagtt
     1321 tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggccgctgtg cggcctctcc
     1381 ttctccccat cacgcgaccg ctcggttttc tcgagctttt atacgcatgc tcctgccgct
     1441 cgccgcagcc gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagaac
     1501 atccctacac tgagcactga cgctctgagg cctcttatga cgaaacaaca gaaaacagcg
     1561 ctgaacatgg ctaaattcat ccagaatcag tctctgctcc tgctggagaa gcttaacgag
     1621 cttgatctcg atgtcgaggc cgacctttgt gagaaactcc acgatgatgc ggagcatctt
     1681 ttccgtacac tgtcctcgag actggatgaa ttacaggatg gtaactgatg gccagagtgt
     1741 ctattagtga agcggcacgg cttgttaagg ttagtcgccc cacaatttac aagatgatta
     1801 acagtgggaa gttaagttac acctctgttg taaagcacgg gaaggctatt aaagttattg
     1861 atacttcaga gcttattaga gcgtttggtt ctcttgatgg tgtaattgat actgtaacat
     1921 atgatgtaaa aagtgacgca gaatctacag gtattaacag cgttggttta catgatttac
     1981 aacatagaat cgctttactt gaagctgaaa acgacggtct taaaggggct gttaaggctc
     2041 gagatgagca tattgattcc cttcgtcagg ctatacagct tctggagcac aagcacgaac
     2101 cttcttcacc accccattcc tcgtggtgga aattctggaa aaaatcctga ccccgtcatg
     2161 cttcagtttt cctctccggc catcccgggg agttcctgaa ccttcccaaa aatcgccaga
     2221 tctctgaacg ctcctcacca ctaaaaatac ccacacccca cgcaaaatta acattttgca
     2281 gatttgtatt taatatcatc agcttacttt actgaaattt cgttttttaa attttattta
     2341 tatagatccg actctatgac gactcgtcat agagtccccg gcgagtcgtc atagagtcgg
     2401 tgacgactca gcactataaa aacaatgagt taataacgac ccgactctag acccgactcc
     2461 ccgtttggtt gttttttagt cgcggcgact ctgggtacga ctctagtgca gagtcgccgg
     2521 tgaatcgtgt gttgagtcgt ccggcgactt gccataacgt gattgccggc cgtcgacgaa
     2581 cccgattcct gaacgtgctg aaaaccggtt ttccggctgc gccggcaacg cttcacggcc
     2641 acgcaataaa ttgcgtcccc gttcaccgtt ttcggatgga aagaacgacg gaaacaggac
     2701 ttaccctggc tgaatggcgt gaattttcgc gctatgcact tgcgtgcata ctcatgcatg
     2761 ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgttttg
     2821 ccggtttccc gtcagaaacg ccctgaggcc gttttagcgg tgcgtacaat catgccttta
     2881 tgttaaatga caaatgcaaa aaagcccccg aaggggcctg aaggtttttc tgtagggagg
     2941 aaaaacaggt gccctgtttg gctgtacccg ttaccggctg acagccacat tccagtcgta
     3001 tacctgattc aggactagcc ggtgtaacca ttgcggcggg cattagcgat gcaatccgct
     3061 cggtttacat agccctgact ggatgcacca acaatacgac cattgctggc cgttctgcgc
     3121 cagcgccatt cgcctttacc atcagtataa aaatcccatg tatcgttcat tctgttttta
     3181 tcctgtgctg tgtggggatc cccaaggttg atataaagca ttcctcctct ttttcaatcg
     3241 ataagagtgt gttttgcgat gcgagtacga taggaatttc tgttacatgt tactcatgca
     3301 atgccgtctc attaggtcta aaggcttctt gtgatgcaca tagactattt tggtatctta
     3361 atagactatt ttttgttgtt atggcattga ttttaaagat ttttgtattt tgttttattc
     3421 tagaaagatt ataataaaaa gacttataag tctttttatt atgtgtcatg attttttcaa
     3481 gatttgagca gaggtgtttt cgggaggaga gtatcatgag tctagaaaag tatgtgaagt
     3541 tactcgagag agttgcagcg atttctggtg agttaaccca gcttgtaatc tctgtgacgc
     3601 tattaataat ttcttggtat cttattaaat aattaaggtg aagctatgcg gtttttatgg
     3661 aggcctttgc cttactctag gttaagggaa tgtgattttt taaactgtat tgatgagaat
     3721 gattcgtctt catggatagc cgcattgaaa atattcattt gcattgttat taaatctgat
     3781 ctggatgaga atggagagta ttgtgcaatg attacgtatc cacaagtcca gaaaatgaca
     3841 gggatgagta ggcagttaat atgttgtggt tttgataagc tgaagtttta taaaatgata
     3901 tctagcgagg gtgttaggaa aaaaaagtat tcattattaa cttgtgaaaa aggctccact
     3961 agacaaaaac ct
//
