LOCUS       NZ_LR890615             1822 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_8D isolate MINF_8D plasmid 10, complete
            sequence.
ACCESSION   NZ_LR890615
VERSION     NZ_LR890615.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357527
            Assembly: GCF_904866345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:33:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,170
            CDSs (total)                      :: 5,046
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 80 of 270
            Pseudo Genes (incomplete)         :: 202 of 270
            Pseudo Genes (internal stop)      :: 50 of 270
            Pseudo Genes (multiple problems)  :: 58 of 270
            Pseudo Genes (short protein)      :: 4 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890615.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_8D"
                     /isolate="MINF_8D"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="10"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            507..1040
                     /locus_tag="JMW69_RS25535"
     CDS             507..1040
                     /locus_tag="JMW69_RS25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893324.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="replication/maintenance protein RepL"
     gene            1053..1340
                     /locus_tag="JMW69_RS25540"
     CDS             1053..1340
                     /locus_tag="JMW69_RS25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023909281.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     Typing          complement(1583..1819)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.597"
                     /coverage="91.0"
                     /evalue="8.74e-88"
ORIGIN
        1 caccgataac atgcgagagg tggcaccgct cgccgcagcc ccagcgaccg aacgttagtg
       61 agcgagtggg cgaggaagcg gaagagaacc ggaaaccaca ttgagcacgt acgcactgat
      121 gcggggcgtt ttagcggtga acggactgca aaaacatgat cgagctgttg aacgtggaga
      181 gcgcgaaatc gggcaaaatc acggctgacg aggcttgcag aagagtaagc cagtatacac
      241 tccgctatcg ctacgtgact gggtcagggc ttcgccccga caccccaaat gttcgatgaa
      301 ctaattaaat cttgtgtagc aaaaaagtgc tttgatagat ctttagagca aaaatcgaca
      361 ttctcttaaa aatcaaagca ttatggcacc aacatacgaa acgttatttg catgtgtata
      421 cgcaaacatg tttgcgtgtg ttgtttcgta atgtacgata taaatttcgt aaggtacgaa
      481 acaacgttgc aggtaaggat gcaagtatgc tggaacagca atttgaagat ttcgaacagc
      541 aaaaacggcg agatcatcag gcacaagatc aatccaaaag tgctaagcaa aaacgagatc
      601 ggttgaatcc acctgactgg tcgcaggtta ctaaggcggg ctggaatatg ttggactcta
      661 tatctgagaa ggttcccaga gcatttaggc tatattcttt ttttgctcaa aatatagata
      721 aatcgtgtgg ggctgttgta tgtgaccaaa aattcttagc tgattatttc ggtgttagtt
      781 taaggacgat ccaatattgg attggagatt tagaaagtat tggtgccatc gtgaaaatac
      841 cagtaggtat ctattatgct tatgcgttag atccgtatca ggtatggcgc ggatataata
      901 aatctaaaaa atatgcagcg tttaacgcaa aaacattaac tcaaaaagat ggcgttattg
      961 atcgcaagtt gcggctcatg ctctctagga gagaagagtt tgaaggctcg cttaagacat
     1021 ctaatcatga cttggactag gtatgatgcg ctatgagaat taaatatgaa tcacgttgga
     1081 aattagaaga actaaaatta gaaatatgta gatcaataga ttcgttaatt gatgaatatc
     1141 atatcgaata tgcaatggga ataaatctat atatgaattg tgaagataag gagtttaatc
     1201 ccgttgatat tgtcaatgaa gatggaaaga atattatgtt tgttgttagc aacaatgaaa
     1261 aaatacaaaa accaaaaaaa agtgcgaaga aaaaaattcc tgcacctgta attgagttag
     1321 ataaatatcg gaaaaagtga tggccataca gagccagcgg caggcggaca atccacagaa
     1381 cattgcatgc aatttctagt tgattgtcta tcctgcattg ctcttttgcc gcctcaaaat
     1441 cccttgcgtc tttttgctcc ccgttttcca gaacgaccga gccgccacgg ttccggcagc
     1501 gccttgagcg caaccggaaa ccgtctgggg agaaccccag acccccatcg ctgtaacatc
     1561 gacggtctac gaccacctac caaccgcgtg gctgcctatt ccgtgcaaat gggctttgca
     1621 gggggctgac gcggttcctg gcgacctgaa atcccccggt taggggtatc ggcgcgcctg
     1681 acctcctaaa accctctgta agcgtttttc gcgccttcca tgccctgatg ggcatttaaa
     1741 acccgtttcg tgcgtctgag agcttctgag agcgtttgaa gagctatctg gcgactcatc
     1801 cccgaaagaa caacagcgga at
//
