LOCUS       NZ_LR890614             4072 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_8D isolate MINF_8D plasmid 9, complete
            sequence.
ACCESSION   NZ_LR890614
VERSION     NZ_LR890614.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357527
            Assembly: GCF_904866345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:33:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,170
            CDSs (total)                      :: 5,046
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 80 of 270
            Pseudo Genes (incomplete)         :: 202 of 270
            Pseudo Genes (internal stop)      :: 50 of 270
            Pseudo Genes (multiple problems)  :: 58 of 270
            Pseudo Genes (short protein)      :: 4 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890614.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_8D"
                     /isolate="MINF_8D"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="9"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            join(4010..4072,1..192)
                     /gene="mobC"
                     /locus_tag="JMW69_RS25520"
     CDS             join(4010..4072,1..192)
                     /gene="mobC"
                     /locus_tag="JMW69_RS25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            725..1426
                     /locus_tag="JMW69_RS25525"
     CDS             725..1426
                     /locus_tag="JMW69_RS25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1684..3831)
                     /gene="mobQ"
                     /locus_tag="JMW69_RS25530"
     CDS             complement(1684..3831)
                     /gene="mobQ"
                     /locus_tag="JMW69_RS25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_057077964.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREVPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(3474..3832)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3833..4009
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          726..1426
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.715"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1688..3918)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.658"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagccgga gcctggacac agcgcagaag gttgttgtgg gtgctgcatt gctggcaaag
       61 gtcagaaagc cggaggaggt gcagttgcgt gcctggttac tccagttcct gaaggcagag
      121 gtgacacgac aggctgatgt gacgcgcata ctgccgctga ttaacgagct ggaggcgctg
      181 ccagaacagt gattgtatcg tccgctcgcc gcagccccag cgaccgagcg ttagcgagcg
      241 agtgggcgag gaagcggaag tgaatcagtc gccaggttga gcacatacgc acggtttttc
      301 ggcaggtgat atgcgctgaa cccgcatgaa tactgggttt ccacagggtt ggagggggtg
      361 tattcgaaac ttgcaattct gctttattgc aacgcattgt ttttattggt tttttgtttt
      421 tagcacccca agttatccac aggctcgccg ttaagttttt aagatcttat ttttagatct
      481 tttttttaaa acctttttag gtcttgtaac accatgattt taaagatgtt ctggcaagat
      541 aagtggagct tttgggggtg aaaggtggag cttttggggg tgaaaggtgg agcttttggg
      601 ggtgaaaggt ggagcttttg ggggtgtaat ccacagaaaa agggggttgc aaaaaaaaaa
      661 cttccaccca tatgataagt ggcgtacagt aaaaaaacgc caccaatggc aacgagcaag
      721 cgaaatgtca gatctgatag cttacaaatc aaacgccctt atcgaggcaa gctacaagct
      781 cactttgcag gaacagcgct tcctgctggt ctgtatcagc cgcctgaaat ccggagaaga
      841 ttccccttcg cctgatgaac aaaaaaccat gaccatcacg gctgcagagt ttcatgatgc
      901 ctttcctgac atgggacggc agcacgctga agctaggctc aaggaagcga tagatcgcct
      961 ctgggacagg tcagtaatca tcaagaacga ggaaaaacga gaagagttcc gctgggtgca
     1021 gtaccgggcg cagtatttca aaggtgaggc gaaagtagat atcaccttct ccgatgccat
     1081 catgccgtat ctgacacagc tcaaggggca gtttacgcgg gtagtcgtga agaacgtctc
     1141 cagcctttcc agcacctaca gcatccgcat ttacgagctg ctccagcagt tccgcagcac
     1201 cggtgacaga acgatagcta tcgatgattt ccgaaccatg cttgccgttg aagataaata
     1261 tcaacaattc aaagacttga ataagatgat aatcaagcca gccatcgccg aactgaacga
     1321 aaaaagcgat ctggttgtaa ctgttgagac gatcaaaaag gggcgcacgg ttgtagccct
     1381 gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc gattttaagg
     1441 cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt actattgaag
     1501 tgaaaaattg cgccaaaaac gctctgatgc agcgaaaaaa gaacccccaa tttttggggg
     1561 cggaaaaaca aaactatgct tgcgttatgt gagtgtgctt cttgcattca tccctgtcga
     1621 tgaacatcga agaacatcaa cgaacataac acgatgttaa acaaaacaaa atattgttca
     1681 cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc ttgcatcttc
     1741 ctcgcgccgc cattcgtccc aggtacgatt gaaacccagc gagcggaaca gatgatccgg
     1801 ctgtttttct ggtgtgttga cggcagcaaa aagcggtacc tcacgggttt tcagatgaaa
     1861 ctccataagt tccggcgcag gtgatatgtg gctgtctgca tcgtgattta cggcaacacc
     1921 ataccctgca taccgtaacc ccttatcggt cagtgcactg atgaccagct ggctgttatc
     1981 ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggacaa caccgcccag
     2041 agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cggttttccc ggtcgacaga
     2101 actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca ccttccttgc
     2161 cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact ccggccatgt
     2221 cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct gcgtcagcag
     2281 ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc cgatttcgct
     2341 tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa agattctggt
     2401 gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt tgtgaagcag
     2461 aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc ccgtaacgac
     2521 tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct gctccgcgcg
     2581 actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt taagcaactg
     2641 ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt caaatgccat
     2701 ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta atttttccat
     2761 ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg ccgcccattg
     2821 catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca ggctggcagc
     2881 atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc gcgtcgcctg
     2941 catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt tactgtcctt
     3001 cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag gaagcccgat
     3061 attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc tgagttcggt
     3121 gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt cgccgcgttc
     3181 tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa taccaggcgt
     3241 tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat aaccggcgtt
     3301 cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg catgtgtccg
     3361 gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgacagccc tcgtggtcag
     3421 catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat cagcaatcat
     3481 gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg cggcatcctg
     3541 cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt tcttccggcg
     3601 ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg caggggcggt
     3661 ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt cgtgagtcag
     3721 tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag ctacggcgct
     3781 gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca tttctgccgt
     3841 aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacaatcgaa acggagtgga
     3901 gattgtataa gtgcgcactt cgtgatttgc cgtgcattat accagctcag tttttaaact
     3961 accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca tgagcagaac
     4021 gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg cg
//
