LOCUS       NZ_LR890612             5221 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_8D isolate MINF_8D plasmid 7, complete
            sequence.
ACCESSION   NZ_LR890612
VERSION     NZ_LR890612.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357527
            Assembly: GCF_904866345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:33:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,170
            CDSs (total)                      :: 5,046
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 80 of 270
            Pseudo Genes (incomplete)         :: 202 of 270
            Pseudo Genes (internal stop)      :: 50 of 270
            Pseudo Genes (multiple problems)  :: 58 of 270
            Pseudo Genes (short protein)      :: 4 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890612.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_8D"
                     /isolate="MINF_8D"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="7"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            join(4881..5221,1..190)
                     /locus_tag="JMW69_RS25445"
     CDS             join(4881..5221,1..190)
                     /locus_tag="JMW69_RS25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_117014864.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            197..430
                     /locus_tag="JMW69_RS25450"
     CDS             197..430
                     /locus_tag="JMW69_RS25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(975..1271)
                     /locus_tag="JMW69_RS25455"
     CDS             complement(975..1271)
                     /locus_tag="JMW69_RS25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001679964.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKYLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            1483..1680
                     /locus_tag="JMW69_RS25460"
     CDS             1483..1680
                     /locus_tag="JMW69_RS25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001335786.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            1698..1982
                     /locus_tag="JMW69_RS25465"
     CDS             1698..1982
                     /locus_tag="JMW69_RS25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(2046..2435)
                     /locus_tag="JMW69_RS25470"
     CDS             complement(2046..2435)
                     /locus_tag="JMW69_RS25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001679965.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNLTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            3284..3472
                     /locus_tag="JMW69_RS25475"
     CDS             3284..3472
                     /locus_tag="JMW69_RS25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249489876.1"
                     /translation="MDNRITAFECADTARRSRTTERSESVSEEAEEHRHLHVLLTHLCD
                     ISHPAWCTFHTIRTDAA"
                     /name="hypothetical protein"
     gene            complement(3625..3816)
                     /locus_tag="JMW69_RS25480"
     CDS             complement(3625..3816)
                     /locus_tag="JMW69_RS25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_116965635.1"
                     /translation="MTKQERTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3880..4203
                     /locus_tag="JMW69_RS25485"
     CDS             3880..4203
                     /locus_tag="JMW69_RS25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4412..>4843
                     /locus_tag="JMW69_RS25920"
                     /pseudo=""
     CDS             4412..>4843
                     /locus_tag="JMW69_RS25920"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3452..3539
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(2883..3436)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.705"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..534
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.315"
                     /coverage="100.0"
                     /evalue="1.75e-82"
     Typing          4194..5221
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.335"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaggcgaac ctggaagaaa tcagaaagca gaacgagagt ctggagaagc tgaacgcgaa
       61 gacatggggc gtgcgttatc acgaggggag caacggacgt tttctggtgt tgcccgaggg
      121 gatgaaagcc gaaacgaact ggacgatgga aaacgggaaa cagaatgccg taaggctggt
      181 acaggagtaa cagagcatga cggagctgga aagacatttg ctgaacgcct tagagcaact
      241 gcaacaggac tatatgcagc ggctgaacga atgggagagc gccttcgtgg aattgcagaa
      301 aatgttttcg cttacgcaac gggacaacgc gatgctgaac gagcgggtca tgcagttgag
      361 tcagcaggtg cagcacttga gcgagcagac agaacgcttg agccagttat acagcgagaa
      421 caggagataa gggacgaacg gctgatacag gaacgcgaac atgcgttatc cctggagcgt
      481 gagcgccagc cggaaataca ggaacggaca ctggatggcc cttcgctggg atggtaatca
      541 gagacaggct aaaatgttgt cagcgcgttt ccggtgcatt ttttgggata atggttggtt
      601 cgggggcacc gggaacgcgc ttcagtcaga ccttgctgaa aacacaggaa cgcgttaaaa
      661 cgcatacggg cgcgattctg gtgtgtcttt tgttgctgtg ttttgcctct attgccagat
      721 cgcgctcatt cccctgaact tctgttttcc cgctccggtc cccgtcttct tcgcttccga
      781 aagcatgaaa aaaatccgtt tcagctgtct ctggctggca attcctgcgt gattcacatg
      841 gctgcatagc tatgcatgtg gttaaaattt accgggtgcg atcgcggcag tttttcgggg
      901 ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ccgaacgcgt ttcagcggtg
      961 cgtacaatga gacgttatgg taaatcaccc ttccaggcgt tcagcccttc ttccggtttt
     1021 tttccgttca ggtatttacg ggcgagacga atgcgtcttg cctggtaaat cgcatcgtag
     1081 gcctgtgact gtcccacacc gttactgatt tcactgacca gatggcgcag gtcggcctgt
     1141 gtcagtgtgt aaacgtgttt ctggatgagg tcacgggctg tccgctcttc gtatccggca
     1201 gcgataagtg cagccagcag gcgtttgtct gttttgctca gtcggtggcg accagatttt
     1261 gttttgctca cgttatctcc tctctgtttt ttctcctgat gttatccggt gctgtttttc
     1321 tgctgcgaat cttcccttcc tgatttttat aagggtgatt cagatcacaa aaatgaaaat
     1381 tcatatcatt gacaggaaaa ataatgcggc gtaatttatg ctgtatataa aactagtgtt
     1441 tatatataca gtatattctg tgtaaataaa gggagggata aaatgaaaga agaaaaagta
     1501 ttgcttcacc gtttcttatt tgtagttagg aataagaatg gttgtgaact atcatgttcg
     1561 gcagacctta tgggaacaag ggatgatgtt tataagtatt tctctgattc tgttagtggg
     1621 ctagatgttg agcttataga tgtttcttgc gagagtgaat gggaagaaca ttcgcattga
     1681 actaatcagg agattatatg atttctcaaa atgtttgggc tcctcctttc ttttttggtc
     1741 cttgggttga acttgaaaat catactggtg atatcgcgta cactgtctat tttgatacat
     1801 tgtctaatgt acccggtcca tttattgctt ttatctggta tgcccctaat gggccaagaa
     1861 cagttaacgt tattggtcca gcatcttatc agatctcttg caagggcgct ggtgttgata
     1921 aaattaaatt taaaagtgtc agtgggacag ggcagattat tcatgtatac actaccttat
     1981 aataagagct gaatagatat aatcctgcct aagctatacc ttaagcagga ttatatctat
     2041 taatattatt ttgaatatgc ttttatgatt tccatcactg ttgtttcatc gttcacaggt
     2101 tgataataat gggttagatt tagtgcagaa tgaatgattg caggttggga tattactttt
     2161 ccatcaaaat atgaaatcag gggtttccct atatttttac tagttatttc gtttagtttt
     2221 ttcttacata aactatctgt gtttacttct attatgacgc ctttgttttt ttctgattgg
     2281 gaagggtaca ttttaagtat gcaatattta ttcagaatga tgctgtcacc tccaactttg
     2341 taaacaatgt ttttgtttgt ttcagtactt gaacatccta taagtaaggt cagtatagta
     2401 ctcgatgtta ttgactttac tgatgatttg aacatattaa ctccatttta ttttgttaaa
     2461 attccaatct ataaatacat atacacgtaa aaataaacaa tgcgaacgtt ttcacttata
     2521 ttatccccat gatgtgattg aatggtggtt ccatataaga gagggcaatt atactatctt
     2581 tctctcctga actgatggga ttgcagttta gagaaaaaag atcaaaggat cttcttgaga
     2641 tccttttttt ctgcgcgtaa tctgctgcct gtaaacgaaa aaaccaccct ggcaggtggt
     2701 ttttcgaagg ttagctaatc ctggcagatt acctaaccgg gtagctggct taagtggagc
     2761 acagatacca aaagatgtcc tttcagtgta gccgctataa ggcttcaact tcaagagttc
     2821 tgcgtatcta aatccacttt ttagttacca atggctgctg ccagtggcgt taagtcgtgt
     2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3061 gtaaacggta aggtcggaac aggagagcgc acgagggagc ttccgggggg aaacgcctgg
     3121 tatctttaag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3181 cgtcaggggg gcggagccta tggaaaaagc gcctccggcg cggccttttc cggggttttt
     3241 gttttttgct cacatgttct ttccggcgtt atctcctgat tgtgtggata accgtatcac
     3301 cgcttttgag tgcgcagaca ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3361 gagcgaggaa gcggaagagc accggcattt gcatgttctc cttacgcatc tgtgcgacat
     3421 ttcacaccct gcatggtgta cttttcatac aatccgcact gatgccgcat gactaagcca
     3481 gtatacactc cgctgtcgct acgtgactgg gtcatggctg cgccccgaca cccgctaaaa
     3541 ccccgctgac gcgccctgac gggcttgtcg gctcccggca tccgctcaca gacaagctgt
     3601 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatccccga agcgagcgag
     3661 gcagctgcgg taaagctcat cggcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     3721 atccgcgtcc agttcgttga gcttttccag cagtgttaat gtctggcttc tgataaagcg
     3781 cgccatgtta agggcggttc tttcctgttt agtcactgat gcctccgtgt aagggggatt
     3841 tctgttcatg gggtaatgat accgatgaaa cgcgagagga tgctcacgat acgggttact
     3901 gatgatgaac atgcccggtt actggaacgc tgtgagggta aacagctggc ggtatggatg
     3961 cggcgggtct gcctgggtga gcctgtcgcc cgttccggga ggctgccgac actggcaccg
     4021 ccgttactgc gtcagctggc cgctatcgga aataacctga atcagacagc gaggaaggta
     4081 aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg tggctgcact gatggccatc
     4141 ggggatgagc tgcgccggct gcgtctggcc gtcagggaac aggggacgcg ggatgatagt
     4201 taagtttcat gcccggggaa aaggcggtgg cagtggtccg gttgattacc tgctgggaag
     4261 ggagcgaaac cgggaagggg caacggtgct tcagggtaat ccggaagaag tccgggaact
     4321 catcgatgcc acgccatttg cgaagaaata cacgtccggt gttctctcgt tcgcggagaa
     4381 ggcgctgcca gcgggaggac gtgaaaaagt gatggcgagt tttgagcgtg tactgatgcc
     4441 cggtctcgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca agggacggct
     4501 tgagctgaat tttgtcattc cgaacatgga gctacagagc ggaaaacgcc tccagccgta
     4561 ctacgaccgg gcagacaggc cgagaattga tgcctggcag acgctggtta atcaccatta
     4621 cgggctgcat gaccccaacg ccccggagaa ccgccggata ctgactctcc ctgataacct
     4681 gccggaaacg aaacaggcgt ttgctgaggg cgtcacacgc gggatagacg cgctttacca
     4741 tgccggagag ataaaaggcc gtcaggatgt gattcaggcg ctcatggaag cagggattga
     4801 agtggtcagg gtgacgcgaa gcagtatcag cattgcagac ccgaacggcg ggaagaatat
     4861 cagactgaaa ggagcattct atgagcaatc ttttacagac gggcgcggag ttcgagaaaa
     4921 agctgaaaga gagagccgaa tctacagaga aaatgctgaa cgacgagttc aggaagctcg
     4981 gcgaatctgt aagcaaggct gtgacatcaa acgagacgaa aatcaaagac gctatagccc
     5041 tgttcacagc ttcgacagag gaatcactga aaaaacaccg ggaaggggtg aaagaggcga
     5101 tgatgcagca caggaaggac gtgttaaagc tggcagggaa tacgggcatg atgttactgg
     5161 ggatggtcat tttcctgttt accgtgagtg gcgggacgct ctggtatctt ggcgggatga
     5221 t
//
