LOCUS       NZ_LR890567             3223 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB2_9A isolate KSB2_9A plasmid 4,
            complete sequence.
ACCESSION   NZ_LR890567
VERSION     NZ_LR890567.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357401
            Assembly: GCF_904865765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:40:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,211
            Genes (coding)                    :: 5,079
            CDSs (with protein)               :: 5,079
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 55 of 132
            Pseudo Genes (incomplete)         :: 80 of 132
            Pseudo Genes (internal stop)      :: 21 of 132
            Pseudo Genes (multiple problems)  :: 21 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890567.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_9A"
                     /isolate="KSB2_9A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2014-02"
     gene            join(3022..3223,1..131)
                     /locus_tag="JMW10_RS26520"
     CDS             join(3022..3223,1..131)
                     /locus_tag="JMW10_RS26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            1149..1472
                     /locus_tag="JMW10_RS26525"
     CDS             1149..1472
                     /locus_tag="JMW10_RS26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1681..>2115
                     /locus_tag="JMW10_RS26860"
                     /pseudo=""
     CDS             1681..>2115
                     /locus_tag="JMW10_RS26860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2150..2635
                     /locus_tag="JMW10_RS26535"
     CDS             2150..2635
                     /locus_tag="JMW10_RS26535"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2639..2854
                     /locus_tag="JMW10_RS26540"
     CDS             2639..2854
                     /locus_tag="JMW10_RS26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          862..1053
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(399..879)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          1463..2952
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtactaat aagcctagct ggttggttgg ttagcggtca aaaacttgat tggcccgcaa
       61 ttgcaggaat gttccttatt tgcgttggag ttttggttat taatattttt tcaaaatctt
      121 ctgcgcatta actttttacc gaagcatatt ggccaaagca aaaggatttc cttgagatcc
      181 tttctttctg cgcgtaatct ttttccctgt aaacaaaaaa accaccgcta ccaacggtgg
      241 ttcgtttgcc ggaattcaga gctaccaact ctttgttccg atggtaactg gctttcacgg
      301 agcgcagata cctaaactgt cctttcagtg tagccgcact caggctttca cttcaagaac
      361 tctgtaggca ttctctacaa tctctgctcc gtgaacccag ttaccaatgg ctaccgccac
      421 tggcgatttg tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg aacgggcgtg
      481 gtagtcggac tgaacggggg gttcgtgcat acagtccatc ctggagcgaa ctgcctaccc
      541 ggaaccgagt ttcaggcgtg gaatgagaaa ccgcggccat aacagcggaa tgacaccggt
      601 aaaccgaaag gcaggaatgc gggggagcac gagggagcca ccagggggaa acgcctggta
      661 tctttaagcc gcatcgggtt tcgccaccgc tgatttgagc gtcattttcg tgatactcgt
      721 caggggggcg gagcctatgg aaaaacggca actccgcatc cttttcttgg cctttcctgc
      781 ttcgagcggc atttgcttat cccgtaatct gtgagctggt aacgcccgcc gcagtctcac
      841 ggcaggagcg tagcgaccga gtgagcgagg aggcgtcatt tcatcaccac ctgcattttg
      901 cacttacgcc cggcagccgc atttttcccc tgtcacatga agcactttca taaattgcca
      961 ctccgtcata acatgtttag ccagtataca ctccgctagc gctacgtgac tggttcaggg
     1021 ctgcgccccg aaacccgcta aaaccactgc cgcgcctgcg gcttgtccaa caccgtgccg
     1081 accgggaaaa gatttcccgt ccgtcccggc gttatcagga ggccggattg gcagacaaac
     1141 gcagcaaaat gctcaccatg tgggtgactg aggacgagca ccggcgtctg ctggagcgct
     1201 gcgagggtaa gcaactcgcg gcctggatgc ggcagacgtg cctggacgag aagcccgcac
     1261 gcgccggcaa acttccgtcg atctcgccgg cgctgcttcg tcagcttgcc ggcatgggga
     1321 acaaccttaa ccagattgcc cgccaggtta acgccggtgg tggaagcgga cacgaccgcg
     1381 tgcagattgt cgccgcgctg atggccatcg atgccggact cgagcggttg cggcatgccg
     1441 tactggaaaa gggtgctgat gatgatcgtt aagtttcatc ctcgggggcg cggcggcggc
     1501 ggcgggccgg tcgattatct gctgggtaaa gatcgccagc gcgacggcgc cagcgttctg
     1561 caggggaagc cggatgaagt ccgggagctt atcgacgcct cgccctacgc caaaaagtac
     1621 acttccggcg ttctgtcttt tgccgaacag gatttaccgc ccggccagcg cgaaaagctg
     1681 atggcgagtt tcgagcgggt tctgatgccc ggactcgata aagaccagta cagcgtgctg
     1741 tgggttgagc accgggacaa ggggcggctg gagctgaact tcctgatccc gaacacggag
     1801 cttctgaccg gcaagcggct acagccgtat tacgaccgcg ccgaccgtcc gcgcatcgat
     1861 gcctggcaga ccatagtgaa cggcaggctg gggctgcatg acccgaacgc gccggagaac
     1921 cggcgggtgc tggtctcgcc ttccgcgctg ccggaagcga agcaggaagc cgcccaggcg
     1981 attacgagcg gcctgcttgc ccttgcctca tccggggagc tgaaaacgcg tcaggacgtc
     2041 actgaggcgc tggaaagcgc aggttttgag gtggtgcgca ccacaaaaag cagcatcagc
     2101 attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccatcta tgaacagtct
     2161 tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga ctaccggcga
     2221 gatgctgaaa gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag
     2281 cgagaagaga atcagcgccg ccatcctcga ccacgaccgg aagctgtcct cagccatgag
     2341 ccagcgcacg aaagggatgc tgcgcatggt cagccagacg tggctgacca tcgttctggt
     2401 ctccgcgctg ctgatagcgt cgagcgcggg cattctgtgg tggcaggggc agcagatact
     2461 cgagaactat acgaccatcc gggagcagaa gagcacgcag gccatgctgt cagagaggaa
     2521 cagcggcgta cagctctcga cctgcggcga gcagagacgc cgctgcgtga gggtgaaccc
     2581 ggaagcggga cagttcggag aggactcgag ctggatgata ctggcgggga aatagcacat
     2641 gacggagctg gaaaaacagt tgctgagcgc attagagcag ctacagcagg actactcgaa
     2701 aaggctggac gagtgggaga acgccttcgc ggaatggcgg acgatgtctg gtcttattca
     2761 acgggagaac gcggcgctga acgagcgcgt cacggtcttg agcaggcagg tgcagagttt
     2821 gagcgagcag ctgcgccggt tgtcgaaagg ctgaacgcga tagaaactca ccggcagcag
     2881 gagcgggcgg tgcagcacca gaaagcgctg gagctggaac gatcgcagtg gcagcatcat
     2941 ggtccttcgc tatgataagc cgggttctat taattagttg ttatgtaaag atatttaaat
     3001 ttttcaacat gaggtatttt tatgaataca tatattttcc tgggaggggc tattgtggct
     3061 gaagtcatag gcaccacctt tatgaaatat tcagaaggat ttacacgcct gtggccgtct
     3121 gtaggaactg caataagtta ttgcgctgcc ttttatctgc tttcccaaac attggaaaat
     3181 atacctaccg gtattgctta cgcaatttgg tctggtgcgg gaa
//
