LOCUS       NZ_LR890566             4064 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB2_9A isolate KSB2_9A plasmid 3,
            complete sequence.
ACCESSION   NZ_LR890566
VERSION     NZ_LR890566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357401
            Assembly: GCF_904865765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:40:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,211
            Genes (coding)                    :: 5,079
            CDSs (with protein)               :: 5,079
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 55 of 132
            Pseudo Genes (incomplete)         :: 80 of 132
            Pseudo Genes (internal stop)      :: 21 of 132
            Pseudo Genes (multiple problems)  :: 21 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_9A"
                     /isolate="KSB2_9A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="3"
                     /geo_loc_name="Australia"
                     /collection_date="2014-02"
     gene            complement(join(3098..4064,1..293))
                     /gene="mobQ"
                     /locus_tag="JMW10_RS26490"
     CDS             complement(join(3098..4064,1..293))
                     /gene="mobQ"
                     /locus_tag="JMW10_RS26490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_099745348.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="mobQ"
     gene            553..984
                     /locus_tag="JMW10_RS26495"
     CDS             553..984
                     /locus_tag="JMW10_RS26495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157769352.1"
                     /translation="MNKKQALWIGGKMNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNE
                     DVFNRCLKLYYAIDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNY
                     INIYELKKLFLLQKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     gene            974..1252
                     /gene="traD"
                     /locus_tag="JMW10_RS26500"
     CDS             974..1252
                     /gene="traD"
                     /locus_tag="JMW10_RS26500"
                     /inference="COORDINATES: protein motif:HMM:NF018152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_099745350.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="traD"
     gene            1385..1651
                     /locus_tag="JMW10_RS26505"
     CDS             1385..1651
                     /locus_tag="JMW10_RS26505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519627.1"
                     /translation="MSQKKEQKTAEKPTAGPLRKHRSRAEKIDDLKKQISELRKAEREE
                     RQRARDEKLIILAKAVYEKAGKPKDIEALIDFVKSEDFKIEYK"
                     /name="hypothetical protein"
     gene            complement(1666..1935)
                     /locus_tag="JMW10_RS26510"
     CDS             complement(1666..1935)
                     /locus_tag="JMW10_RS26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_099745351.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(1932..2789)
                     /locus_tag="JMW10_RS26515"
     CDS             complement(1932..2789)
                     /locus_tag="JMW10_RS26515"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_099745352.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication initiation protein"
     Typing          complement(3102..4063)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcacaaggca aagttatttt aaattctttt gctgttcttg cgtcctttct cttctctgtt
       61 ttttcaacat agttccatag atcattgctt gatttaatca aaaaatctgg cgttccttct
      121 ggtgccaata tttcagcata aaccagatcg tcacgattag ctgatttctc gccgtgtatt
      181 tctccggttc gttcatctat catgacggaa cgatgacgat aagcagcttt tgcaactgag
      241 ctgacaccat ccgaacgact aacgttctgc atttctaatc tatataaagc catataatta
      301 tatcctcatg agaaccccta ttttttcttt tgggtttggg ttttagtttt tcgtaagaaa
      361 aacaggtttt agccgtcggc agaccccggc attgtttaca atgcgtaacc gggcattcct
      421 tagcttttat ttttcgctaa cgctcaaact aaaagaggaa tgaaaaacaa actagctttt
      481 ctttcactga tatatataaa ttaactctga ttaaattaaa gtcaaatttg acaatattta
      541 aaagttacga tattgaataa aaaacaagca ttatggattg gtggtaaaat gaataaaaaa
      601 gaaatagaaa aaaggctatt gatttttaca agcgagatcg acattgaaga tattaaaaac
      661 gatttcatca atgacgaagt taatgaagat gtttttaata ggtgtttaaa gctgtattac
      721 gctatcgatg atgcaatcta taacaataaa aaatcattgt ttgaagcgtt gatatatttg
      781 ataaataaag atcgtgaaaa taaagatcat gatgaaccaa ttataactga ttatgatctt
      841 gtgtatttaa cactaaataa ttacataaat atctatgaac tgaaaaagtt gtttttatta
      901 caaaaaaata tcaatgattt tgattttaaa agtatgattt cagagataga aaaaattaaa
      961 agagaggttg agtatgggaa gtaaaaaaac gttagagcaa agaatggcac ttgcacaaga
     1021 tagattatct aaacttaaaa aagaagcatc gcagaaagaa agaaaagcta gagaacataa
     1081 aaaatttact gttggcggtg aagttacaag ggtgtttggt ttagattacg atctagctct
     1141 tgtagtgggt tttcttgaac actttaacaa gttaagcgat gagcaaaaac aagcgttaaa
     1201 aaacaatggt gaaagattgc taaaatcacg tctagaaaac gctgacaagt gacagatttc
     1261 taatctgaca cttggaaggg ttgaacaagc aagaacccgc cgctggcggg gcttgtgcgt
     1321 tagtcttcac cgtagcccgg ctttgtgata tgtttaaaaa acatatcatc cgggagagta
     1381 aaaaatgtct caaaaaaaag agcaaaaaac agcagaaaaa ccaactgcag ggccacttcg
     1441 taaacacaga tctagagctg aaaaaataga cgatttaaaa aagcagatta gcgaactaag
     1501 aaaagcagaa agagaagaac gacaacgagc aagagacgaa aaattaatta ttcttgcaaa
     1561 agctgtttat gaaaaggcag gaaaaccaaa agatattgaa gcattaattg atttcgtaaa
     1621 gtctgaagat ttcaagattg aatataaata aagctaaggg gcattttagc ccctgttttc
     1681 ttggtcgatt tgtttgtatc ttttggctaa tgctttaacg gcattttctg ttgtattcat
     1741 tttttcagct acttcacgcc attttaagcc ttttttatgg tgtaacgtat aagctgtttt
     1801 tcttctgttt tctgcttctg attcataatg ttctcttggt tgagaagtgt atttttttac
     1861 agtgcttaaa gaaagattga actttttagc catctctttt tgtgttaagc cctttcttat
     1921 ctcgttgcct ttcatttttt tactcctata aattctataa gtttttttgc ttttgtattg
     1981 gttttagttt tacctttcgc tttctttgct ctgttgcttt gtatttcgct gaaagtatca
     2041 ctatcaaaag tcccccatac ccaagatgaa atgcttctta ttgtgtggtt tatttcttta
     2101 aagcttagtt gatcttcgaa tgaatcgttt atgttttcag cttcttcttg taatgctgaa
     2161 atgaaaccgc agaaatcatc atttttttga tatttaaaaa ttatgctgta agcgtaaaaa
     2221 cgcagactat caaataacgc tgtattcctt ccctctgcaa ctattgattt tcttacttct
     2281 ttttttactc ttttatttgt atctatgaac tcacatagat agttcaattc aaatcttggt
     2341 tgttcgttcc aaaatgtacg ccatctcgga ttgagagggt tttttgttat cttgcccgta
     2401 taacaaggat ctgcacctaa tctttcggtg agtccttcta taacgccttt taataacttt
     2461 tgtggctttc ttctggcgtt ttctgttata caaacaggag atgataaggc ataaaataaa
     2521 tgagcgtgac cattagccgg attttgagtt ataaagttag gcttcggtat atctgcatct
     2581 tgccatgcaa tagccgctgt atcgctgtca atatcaaaca caatagagtt aatgaagcta
     2641 tcgttgttat gctcgatata aacgctgttt aaagctcgtc ttttgtttgt tctataaata
     2701 ccatctcttt tgaagtcgtc agtgcaaaat ggtcttgata taatttgatt tataaaatca
     2761 cttctgctat attctttttg tagtatcatt ttttaataca atccttttat tgtagatccc
     2821 tgattttctc ctgtcgccaa acattcggaa atcagggatt tttgttttta tattactgtt
     2881 ctaacataac ctttcatggt gtcaagtagt caccactaaa aaagtgtcaa gtggtcattt
     2941 tagccatatc aggtaataat gggtctgggg gtatttttgc gaagcacagg ccgaaggctt
     3001 caaatcatca caagttaagc tatagcctga taggtacagg ccgtaggcta tgagcgaagc
     3061 cctgtaatct tatgtaggat ctggctgtgc tggttagcta accgttgtca ctgctgtctt
     3121 cctcgacaac tcgacgctta cgagaacccg gaaccggcac agacttcacc tttaacgatt
     3181 taccctctga tacgtctaaa acgtcctgta acgcattctg agccgttttt agcccttccc
     3241 cgtccgattg cttaacctga tatgagatcg caggctgtgg agtccttacg tttgccctgt
     3301 cactcatctc ttgccatttc gctttggtga ttggtaaacc cgtcaccgga tcagtcatta
     3361 aatcgtctga tgcatggaca tcgtttttaa tatctttttt agttgagaac ttagctttaa
     3421 tattttttat aaaattaaca gtagaacgcc accaagattt tttttctgaa aagtcattat
     3481 tttttgactg ctcttttttc tctaacggat gaaccacgaa tgaagcaaca tcaacaactg
     3541 caacacctct caacaacctg aaagccctag cgtcggctct cgccctgtct ctgatagctt
     3601 ggtcacgttt gcgttgttct tgaccttctt ttgttctcca ttttttacgt gataagttct
     3661 gcattggtgg gcgatctagt tcaatagctt ttgctagata taaaatagac ttgtcaaaat
     3721 cttttgcatc ttctgctttt ttagctaaat cgatcattat atttttttgt tcttctagtg
     3781 ttctatgact gacgcgagat tttaagcctg catttttcaa atgctgattt tgaacttttg
     3841 cccattgttt acgccaatca tgaagggttt tttcttcatc ccatgacctt acttttttac
     3901 caaatcctga aagcgttatt tctcttgtcg taatcatcac atgagcgtgc gggtttttat
     3961 tgtctttgtc gttatgtata acaaagtcgc aaatgatgcc tttactaaca aagttttttg
     4021 ttaagtattc tttcaatagt tttatatttt gttcattggt aagt
//
