LOCUS       NZ_LR890540             2102 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_8B isolate MSB1_8B plasmid 5, complete
            sequence.
ACCESSION   NZ_LR890540
VERSION     NZ_LR890540.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357369
            Assembly: GCF_904865655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:21:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,430
            CDSs (total)                      :: 5,309
            Genes (coding)                    :: 5,098
            CDSs (with protein)               :: 5,098
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 77 of 211
            Pseudo Genes (incomplete)         :: 144 of 211
            Pseudo Genes (internal stop)      :: 44 of 211
            Pseudo Genes (multiple problems)  :: 49 of 211
            Pseudo Genes (short protein)      :: 5 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890540.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_8B"
                     /isolate="MSB1_8B"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="5"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            complement(102..368)
                     /locus_tag="JMX06_RS26865"
     CDS             complement(102..368)
                     /locus_tag="JMX06_RS26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(372..1337)
                     /locus_tag="JMX06_RS26870"
     CDS             complement(372..1337)
                     /locus_tag="JMX06_RS26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(713..944)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ctgaggataa tgctgaggat aatgcaggat agacagagca ctagagattg catgcaatct
       61 cgtacctatc acctcagcct gccgctggct cttttccaga actaagaatg cgttcttaaa
      121 aatccaaact tgataccgtt taaatctatt ttcacaaaaa gagtttttac cctctcgttt
      181 gttttctgaa tatataaaat taattcatcc tcaaaacctt cgtgcggatg aaaaccaaat
      241 gtgccaaaac ttaatccatc aataagcaaa ataaggtttt taaacctgtc aggaatcacc
      301 gcttcacttc ttaaaaaaaa taaaccaata gtcttatttc cattcaggta ttcaatatta
      361 atattcatac gttaatccga tttatccttc tcaggggcgc gtttgtaacg ccgtttacct
      421 gaatcccaga caaacgccgt ccgcttcccg tcatcagtgc catcccctac atcgtcggca
      481 atgacaagat cctcattggt ttctcgatcc aactggagga cgttttttag cgccccaccg
      541 gtcgagataa aacggcgctt gtgaagctgc cgcgtcagct caagaaacca ctcaggatcg
      601 tttgccatat cttccggttt aacggagtat ttcagcgttt cagcaaccgc gctttgcagt
      661 tgctcggcaa cgttggccac aacctcacct gtcttagttt ttactgcccg aatatcgata
      721 ttcggctcat agttcacccg caagcaatcg cgccagagtt ctacccaacg ttcgtgctta
      781 acgtagttct tccctttaaa ccaagaaggt tgcaccatca gcagacagtg aaaatgcgga
      841 tgtgcgctgc catccttacc tcgcgtcacc tccgtagccc tgatccaccc ctgaacaggt
      901 gatagctcct ttcgcttttc cattcgctta aacgccgcat tcattgctgt aaggactgtt
      961 ccaagttcac ctatctcgca gttcctgaca gttaacgtca gaaacaacca tcgggaagac
     1021 gggtaatcca caacgatttt cggtagagcc tgataaaaac gggcttgcca catgagggtt
     1081 cttctccact ggcagacagg gcaatgacgg acacggcaaa attgcgcact acgaagccgt
     1141 aggcgcgttt ctccggtttc catgatggta gaccaaccaa aacgtaacaa accgctacag
     1201 gaggccatac ggagtgcata gcgctcgaat tcagcagcag tctggtagat acccccgaca
     1261 gaatcggtaa gcgtgcgctg ggtatcccaa actgcatcac gggggctgta gtccgaaagg
     1321 aatttatctt ctgacattaa gcctgctccg atctgggcag gctgtgattt gatgtggtat
     1381 attcatcacg tacctgtgca ttgcgagcca gtacatgtca ccagagtttg ccgctgaggt
     1441 gacacattcg aaatgttggg gagtgttgac gcactcccca acgccgatta aaaacgcctc
     1501 aattctgctc cattttcttt gacctgtcaa aacccactca aagcctcgtc attactgggc
     1561 taaaccacta tttcatatcc gccatttatc ccttaagtat aaatacaagg atcacgcgcg
     1621 agaattgcgc aaaaaataac cacaatttta acccccccaa aacaagcacc cccgaacggc
     1681 ttaccccaac cttttccaca cacccgatca ccaaaacagg gaataaaaac aaaaccctgt
     1741 cttacaggtg gatggcctca cttcgtgaaa ccatccaccc cgcataagtt ttttaatcgg
     1801 tggtgtcgtc aatcactaaa tcggaacccc aaaggggacc cccacgattt agcatttgac
     1861 gagattaact ctcggagatg gcatcatgct tttaacggac tttaaaaaac ggtgaacgga
     1921 tgcggatttt aatccgtgtc tgtgaggcgt tgagcaacga gcaaagcgag gcggtagaaa
     1981 gagagccagc ggcaggctca ggacgataat ccggggaaaa tgtaccactc gcagagtgtt
     2041 aacatttgac atcggattgt ctatcctgca ttggtgtcga gttggcaagg ctgaggataa
     2101 tg
//
