LOCUS       NZ_LR890494             5087 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF018 isolate INF018 plasmid 2,
            complete sequence.
ACCESSION   NZ_LR890494
VERSION     NZ_LR890494.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3356961
            Assembly: GCF_904865825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,096
            CDSs (total)                      :: 4,978
            Genes (coding)                    :: 4,878
            CDSs (with protein)               :: 4,878
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 38 of 100
            Pseudo Genes (incomplete)         :: 59 of 100
            Pseudo Genes (internal stop)      :: 18 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890494.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF018"
                     /isolate="INF018"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="2"
                     /geo_loc_name="Australia"
                     /collection_date="2013-04"
     gene            complement(456..1433)
                     /locus_tag="JMV82_RS25280"
     CDS             complement(456..1433)
                     /locus_tag="JMV82_RS25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="WP_102016631.1"
                     /translation="MARNATNKLLQKAKKLKSDEFYTQYCDIENELQYYKSQFSGKSVY
                     CNCDDPRVSNFFKYFSVNFHELGLEKLIASCYVERDVDIFSNGVVKNGFYYEYRGESKE
                     GCVLDDIKPSYFDGDGDFRSSESIELLKKSDVVVTNPPFSLFREYLEQLIKYEKKFLII
                     ANVNSITYKEVFKLIKDNKLWLGVHLGRGVSGFIVPEHYELYGTEARIDASGNRIISPN
                     NCLWLTNLDTFIRHEDIPLTKKYFGNEAEYPKYDNFDAINVNKTKDIPSDYKGVMGVPI
                     TFLHKFNPDQFELIKFRKGMDGKDLSINGKCPYFRILIKNKRLQ"
                     /name="adenine-specific methyltransferase EcoRI family
                     protein"
     gene            complement(1471..2304)
                     /locus_tag="JMV82_RS25285"
     CDS             complement(1471..2304)
                     /locus_tag="JMV82_RS25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196747.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcoRI family type II restriction endonuclease"
                     /protein_id="WP_102016632.1"
                     /translation="MSNKNQSNRLTDQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKDA
                     LSKEYPQLSFRYRDSIRKTEINESLKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDHDEW
                     RVVLVTEAKHQGKDIINIKNGVLVGKSSDQDLMAAGNAIERSHKNISEIANFMLSESHF
                     PYILFLEGSNFLTENILITRPDGRVVNLEYKSGMLNRLDRLTAANYGMPINSNLCVNKF
                     VKHKDKNIMLQAASIYTQGNGEKWDSKIMFEIMFDISTTSLRVLGRDLFDQLTSK"
                     /name="EcoRI family type II restriction endonuclease"
     gene            complement(2740..2955)
                     /locus_tag="JMV82_RS25290"
     CDS             complement(2740..2955)
                     /locus_tag="JMV82_RS25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2962..3459)
                     /locus_tag="JMV82_RS25295"
     CDS             complement(2962..3459)
                     /locus_tag="JMV82_RS25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3500..3928)
                     /locus_tag="JMV82_RS25645"
                     /pseudo=""
     CDS             complement(<3500..3928)
                     /locus_tag="JMV82_RS25645"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4137..4478)
                     /locus_tag="JMV82_RS25305"
     CDS             complement(4137..4478)
                     /locus_tag="JMV82_RS25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4573..4746)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          2..302
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.555"
                     /coverage="91.0"
                     /evalue="6.94e-67"
     Typing          4713..5087
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.351"
                     /coverage="96.0"
                     /evalue="7.910000000000004e-156"
     Typing          complement(2625..4147)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4144..4457)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.34e-58"
ORIGIN
        1 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgcctctt
       61 ccggtaacta tcgacttgag tccaacccgg aaagacacga caaagcgcca ctggcagcag
      121 ccattggtag acagaatgtg cgagagatgt atcgagagtt cttgaagtag tggcctaact
      181 gaggctacac tggaaggacg aatttggtta catgtctcgc acaagactgt taccacggtt
      241 agagaatctg ccaggattat ctaaccttcg aaaaaccacc tgccagggtg gttttttcgt
      301 ttctggtgct gaagattacg acgaccagct aatgatctca agaaaaacct aacaaaaccc
      361 tacacatcac gttgttacaa tgagaccaaa ggcagctttt aataaataat tcggctttta
      421 ctataaagct ctaccgcata agcccacatt aatcactact gtaacctttt gttttttatt
      481 agaatcctga agtatgggca cttcccatta atagataaat ctttgccgtc cattcccttt
      541 ctaaatttta tcaattcaaa ctgatcagga ttaaatttat gtaggaatgt tataggcact
      601 cccataaccc ccttgtaatc tgatgggatg tcttttgttt tatttacatt tatagcatca
      661 aaattatcat atttaggata ctcagcttca ttaccaaaat attttttggt aagaggtata
      721 tcttcatgac ggataaaagt atccaagttt gtcagccata gacagttatt tggtgaaata
      781 attctatttc cactcgcatc aatccttgcc tcggtcccat ataattcata atgttcagga
      841 actataaatc cggaaacccc cctcccgaga tgaactccaa gccaaagttt attgtcctta
      901 atgagtttaa acacttcttt atatgttatt gaattaacat tggcaattat aagaaacttc
      961 ttctcatact taattaattg ttcaagatac tctctaaaca acgagaacgg cggattcgta
     1021 acaacaacat ctgatttttt tagcagttct atactttcag aactgcgaaa atcaccatct
     1081 ccatcaaaat aagatggttt aatatcatct aaaacacacc cctccttact ttccccacgg
     1141 tattcataat aaaaaccgtt cttaaccact ccgttgctaa aaatatccac atctctttca
     1201 acatagcaag atgctattaa cttttccaag ccaagctcat gaaaatttac tgagaaatat
     1261 ttaaaaaaat tactcactct aggatcgtca caattgcaat aaacagattt cccagaaaat
     1321 tgggatttgt agtattgaag ttcattttca atatcacaat actgagtgta aaattcatcg
     1381 cttttcaact ttttagcttt ttgcagtaat ttgtttgttg catttctagc catttaaaaa
     1441 ataccttaca tatcaatact ttattacaca tcacttagat gtaagctgat caaacaagtc
     1501 tcgacccaac actctgagcg aagttgtaga aatatcaaac attatttcaa acataatttt
     1561 tgaatcccat ttttctccat tcccttgagt atatatagac gctgcttgta gcataatgtt
     1621 tttatcttta tgttttacaa acttgttaac acaaagatta ctatttatag gcatcccgta
     1681 attagctgca gtcagtcggt ctaacctatt taacatgcca gacttatatt caagattaac
     1741 aacccttcca tccggtcttg ttattaaaat attttccgtt aaaaagttag aaccttctaa
     1801 aaaaagtata tacggaaagt gactctcaga aagcataaaa tttgctattt ctgatatgtt
     1861 tttatgagac ctttcgatag cattaccagc agccattaaa tcttggtcgc tggatttccc
     1921 aaccaataca ccatttttta tatttattat atctttacct tgatgtttgg cttccgtaac
     1981 aagcacaact ctccattcat catggtcgtc tttaacctct acaattccac catcaggctt
     2041 gatgctggaa tttgaaacaa aaagagtccc tcctagatca gggtcgattt tttttaagga
     2101 ttcatttatt tccgttttcc ttatactatc tctatatcga aaagataatt gaggatattc
     2161 tttgctaaga gcatccttta ctaattttga aacttcgcca accgcgagat catgagcttt
     2221 tgcataatcc ccaaaaatcc caattacacc ttgagataac ttatgttgat cagttaatct
     2281 atttgactgg ttcttgttag acatgtatca atctttccac tgttatttta tatatttacg
     2341 tattatcgat aggttaccat ataattaatt taccataaca tctgttaccc gcaccgcgcc
     2401 ggaggcactc agggcgtttc tgatggctga cgggcaaaag gtggtggcaa gtacaccgaa
     2461 aaccaccaga aacgcacctg atttatttta cgcatatgca tcacttattc acatcactgc
     2521 atagccggtg atttcggtta aaaaagactc tctgtgtgct gaaatcgcag aaaatggcca
     2581 tgatccccgt cacgctcagg ccacctgaga cgggcgttta tcagtctcca tactccctgt
     2641 caccatgaga taatgtgcgt tgccgttccg gtaccagatc tgctgtcttc tctggttcct
     2701 tttccgttct ttgcccgtat cgctgttgct caatctcatt cagcctgtcg ataaccggcg
     2761 cagctgctcg ctcaaactct gcacctgcct gctcaagccc gacacgcgct cgctcagcgc
     2821 cgcgttctcc cgttgcataa gaccagacat cttccgccat tcctcgaagg cgctctccca
     2881 ctcgtccagc cttctcgagt agtcctgctg tagctgctct aacgcgctca gcaattgttt
     2941 ttccagttcc gtcattctgc tctactcccg taccagcttc accgcgttcc gcgtcttgtt
     3001 gtccaccgtc cggcccgttt cggctttcat cccctccggc agcaccagaa accgtccgtt
     3061 gcggttctcc agataggtca cgccccacgt ctgcatctgc agtttcgaca gcgtatctct
     3121 ctgctgcctg atttccgcca ggttcgccgc tatccggttc ccctggtacc acagcacccc
     3181 gctcaggctg gcgaacagca gccctgacac catggtgatg gtcagccacg tccgcccgac
     3241 cattcgcagc acgctcagcc ggttcgactg catcgccgcg ttcatcccct gttcgtgggc
     3301 gctgatggcg tcgttgattc tcttcgcgct tgcgttcagt tccgatttga caacgttctc
     3361 gtgcttgctg aatgcggctt tcagcctctc ttcggtaagc tgctgctgtg ctttcgattt
     3421 ctcctcgaag tccctcgagg ccgttaaaag actgctcata aatggctccc ctcagtcgga
     3481 tatttcgccc cccgtccggg tcggcaatgc tgatactgtt ttttgtggtt ctgaccacct
     3541 caaaaccggc cgctgtgagc gcccctgtga cgtcatcacg cgttttcagc tctcctgatg
     3601 aggctaacat cagcaaaccc cgtgtaatcg tctctgcggc ttcctgcttc gtttctggca
     3661 gtcctgacgg cgtgaccagc gcccgccggt tctccgggtc gttcggatca tgtagcccca
     3721 gcctgccgtt gaccactgtc tgccacgcat cgatgcgcgg ccggtcggca cgatcgtaat
     3781 acggctggag gcgtttcccc gtcagtaatt cggtattggg gatcagaaaa ttcagctcaa
     3841 gacgaccctt gtctgcgtgt tccacccaca gaatgctgta ctgatcttta tcgagtccgg
     3901 gcatcagaac ccgctcaaag ctggccatca gtttttcacg ctgacccggc ggcaggtccg
     3961 cctccgcgaa tgacagcacc cccgaggtat attttttcac atacggcgac gcatcaatca
     4021 gctcccggac ttcttccggc ttcccctgca ggacgctggc accttcacgc tgccggtctt
     4081 tacccagaag ataatccaca ggaccggcgc ccccgccgcg accccgggga tggaatttaa
     4141 caatcatcgt ccggcccttt ttccagtacg gcatgccgca accgttccag accggcatcg
     4201 atcgccatca gcgctgccac gatctgcacc cggtcatgac cggcaccacc ggcgttcact
     4261 ttccgggcga tctggttcag gttattgccc atgccagcaa gctgacgcag cagcgcgggc
     4321 gaaatcgagg gaagcctgcc ggcgcgtgtc ggtttctcat caaggcaggt ctggcgcatc
     4381 cacgccgcca gttgttttcc gtcgcaccgc tcaagcaacc gccggtgttc gtcttccgtc
     4441 acccacatcg tgagcatctt gttgcgttta tctgccagaa tcgcctcctg ataacgccgt
     4501 gacggacggg aaagcctttc ccggtcggcg cggggttgga caagccgcag gcgcgtcagt
     4561 ggcttttagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta gcggagtgta
     4621 tactggctta ctatgttggc acggatgcgg atatcagtga agtgcttcat gtggaaggag
     4681 gaaaaaggtg gcaccggcgc gtcagcggaa tatgcgatac aggatatatt ccgcttcctc
     4741 gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtgcggg cttacgaacg
     4801 gggcggaaat ttcctggtag atgctaggaa ggaacttaac agggaaacga taaggccgcg
     4861 ccaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     4921 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
     4981 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     5041 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcag
//
