LOCUS       NZ_LR890488             3223 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB2_10B isolate KSB2_10B plasmid 5,
            complete sequence.
ACCESSION   NZ_LR890488
VERSION     NZ_LR890488.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357419
            Assembly: GCF_904865795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,387
            CDSs (total)                      :: 5,269
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 53 of 132
            Pseudo Genes (incomplete)         :: 82 of 132
            Pseudo Genes (internal stop)      :: 21 of 132
            Pseudo Genes (multiple problems)  :: 21 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890488.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_10B"
                     /isolate="KSB2_10B"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="5"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            461..784
                     /locus_tag="JMX01_RS26785"
     CDS             461..784
                     /locus_tag="JMX01_RS26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            993..>1427
                     /locus_tag="JMX01_RS27170"
                     /pseudo=""
     CDS             993..>1427
                     /locus_tag="JMX01_RS27170"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1462..1947
                     /locus_tag="JMX01_RS26795"
     CDS             1462..1947
                     /locus_tag="JMX01_RS26795"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1951..2166
                     /locus_tag="JMX01_RS26800"
     CDS             1951..2166
                     /locus_tag="JMX01_RS26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2334..2666
                     /locus_tag="JMX01_RS26805"
     CDS             2334..2666
                     /locus_tag="JMX01_RS26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             2335..2666
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          174..365
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(2..191)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.513"
                     /coverage="93.0"
                     /evalue="3.33e-30"
     Typing          complement(2701..3223)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.143"
                     /coverage="99.0"
                     /evalue="8.31e-121"
     Typing          775..2264
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgatttga gcgtcatttt cgtgatactc gtcagggggg cggagcctat ggaaaaacgg
       61 caactccgca tccttttctt ggcctttcct gcttcgagcg gcatttgctt atcccgtaat
      121 ctgtgagctg gtaacgcccg ccgcagtctc acggcaggag cgtagcgacc gagtgagcga
      181 ggaggcgtca tttcatcacc acctgcattt tgcacttacg cccggcagcc gcatttttcc
      241 cctgtcacat gaagcacttt cataaattgc cactccgtca taacatgttt agccagtata
      301 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaaaccact
      361 gccgcgcctg cggcttgtcc aacaccgtgc cgaccgggaa aagatttccc gtccgtcccg
      421 gcgttatcag gaggccggat tggcagacaa acgcagcaaa atgctcacca tgtgggtgac
      481 tgaggacgag caccggcgtc tgctggagcg ctgcgagggt aagcaactcg cggcctggat
      541 gcggcagacg tgcctggacg agaagcccgc acgcgccggc aaacttccgt cgatctcgcc
      601 ggcgctgctt cgtcagcttg ccggcatggg gaacaacctt aaccagattg cccgccaggt
      661 taacgccggt ggtggaagcg gacacgaccg cgtgcagatt gtcgccgcgc tgatggccat
      721 cgatgccgga ctcgagcggt tgcggcatgc cgtactggaa aagggtgctg atgatgatcg
      781 ttaagtttca tcctcggggg cgcggcggcg gcggcgggcc ggtcgattat ctgctgggta
      841 aagatcgcca gcgcgacggc gccagcgttc tgcaggggaa gccggatgaa gtccgggagc
      901 ttatcgacgc ctcgccctac gccaaaaagt acacttccgg cgttctgtct tttgccgaac
      961 aggatttacc gcccggccag cgcgaaaagc tgatggcgag tttcgagcgg gttctgatgc
     1021 ccggactcga taaagaccag tacagcgtgc tgtgggttga gcaccgggac aaggggcggc
     1081 tggagctgaa cttcctgatc ccgaacacgg agcttctgac cggcaagcgg ctacagccgt
     1141 attacgaccg cgccgaccgt ccgcgcatcg atgcctggca gaccatagtg aacggcaggc
     1201 tggggctgca tgacccgaac gcgccggaga accggcgggt gctggtctcg ccttccgcgc
     1261 tgccggaagc gaagcaggaa gccgcccagg cgattacgag cggcctgctt gcccttgcct
     1321 catccgggga gctgaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg
     1381 aggtggtgcg caccacaaaa agcagcatca gcattgccga cccggacggg gggcgaaaca
     1441 tccgactgaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
     1501 aaatcgaaag cgcagcagca gactaccggc gagatgctga aagccgcatt cagcgagcac
     1561 gagaagtctg tcagagcgga actgagcgaa agcgagaaga gaatcagcgc cgccatcctc
     1621 gaccacgacc ggaagctgtc ctcagccatg agccagcgca cgaaagggat gctgcgcatg
     1681 gtcagccaga cgtggctgac catcgttctg gtctccgcgc tgctgatagc gtcgagcgcg
     1741 ggcattctgt ggtggcaggg gcagcagata ctcgagaact atacgaccat ccgggagcag
     1801 aagagcacgc aggccatgct gtcagagagg aacagcggcg tacagctctc gacctgcggc
     1861 gagcagagac gccgctgcgt gagggtgaac ccggaagcgg gacagttcgg agaggactcg
     1921 agctggatga tactggcggg gaaatagcac atgacggagc tggaaaaaca gttgctgagc
     1981 gcattagagc agctacagca ggactactcg aaaaggctgg acgagtggga gaacgccttc
     2041 gcggaatggc ggacgatgtc tggtcttatt caacgggaga acgcggcgct gaacgagcgc
     2101 gtcacggtct tgagcaggca ggtgcagagt ttgagcgagc agctgcgccg gttgtcgaaa
     2161 ggctgaacgc gatagaaact caccggcagc aggagcgggc ggtgcagcac cagaaagcgc
     2221 tggagctgga acgatcgcag tggcagcatc atggtccttc gctatgataa gccgggttct
     2281 attaattagt tgttatgtaa agatatttaa atttttcaac atgaggtatt tttatgaata
     2341 catatatttt cctgggaggg gctattgtgg ctgaagtcat aggcaccacc tttatgaaat
     2401 attcagaagg atttacacgc ctgtggccgt ctgtaggaac tgcaataagt tattgcgctg
     2461 ccttttatct gctttcccaa acattggaaa atatacctac cggtattgct tacgcaattt
     2521 ggtctggtgc gggaattgta ctaataagcc tagctggttg gttggttagc ggtcaaaaac
     2581 ttgattggcc cgcaattgca ggaatgttcc ttatttgcgt tggagttttg gttattaata
     2641 ttttttcaaa atcttctgcg cattaacttt ttaccgaagc atattggcca aagcaaaagg
     2701 atttccttga gatcctttct ttctgcgcgt aatctttttc cctgtaaaca aaaaaaccac
     2761 cgctaccaac ggtggttcgt ttgccggaat tcagagctac caactctttg ttccgatggt
     2821 aactggcttt cacggagcgc agatacctaa actgtccttt cagtgtagcc gcactcaggc
     2881 tttcacttca agaactctgt aggcattctc tacaatctct gctccgtgaa cccagttacc
     2941 aatggctacc gccactggcg atttgtcgtg tctttccggg ttggactcaa gttgatagtt
     3001 accggaacgg gcgtggtagt cggactgaac ggggggttcg tgcatacagt ccatcctgga
     3061 gcgaactgcc tacccggaac cgagtttcag gcgtggaatg agaaaccgcg gccataacag
     3121 cggaatgaca ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccaccagg
     3181 gggaaacgcc tggtatcttt aagccgcatc gggtttcgcc acc
//
