LOCUS       NZ_LR890487             4064 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB2_10B isolate KSB2_10B plasmid 4,
            complete sequence.
ACCESSION   NZ_LR890487
VERSION     NZ_LR890487.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357419
            Assembly: GCF_904865795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 15:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,387
            CDSs (total)                      :: 5,269
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 53 of 132
            Pseudo Genes (incomplete)         :: 82 of 132
            Pseudo Genes (internal stop)      :: 21 of 132
            Pseudo Genes (multiple problems)  :: 21 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890487.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_10B"
                     /isolate="KSB2_10B"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            join(2888..4064,1..83)
                     /gene="mobQ"
                     /locus_tag="JMX01_RS26755"
     CDS             join(2888..4064,1..83)
                     /gene="mobQ"
                     /locus_tag="JMX01_RS26755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_099745348.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="mobQ"
     gene            392..1249
                     /locus_tag="JMX01_RS26760"
     CDS             392..1249
                     /locus_tag="JMX01_RS26760"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_099745352.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication initiation protein"
     gene            1246..1515
                     /locus_tag="JMX01_RS26765"
     CDS             1246..1515
                     /locus_tag="JMX01_RS26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_099745351.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(1530..1796)
                     /locus_tag="JMX01_RS26770"
     CDS             complement(1530..1796)
                     /locus_tag="JMX01_RS26770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519627.1"
                     /translation="MSQKKEQKTAEKPTAGPLRKHRSRAEKIDDLKKQISELRKAEREE
                     RQRARDEKLIILAKAVYEKAGKPKDIEALIDFVKSEDFKIEYK"
                     /name="hypothetical protein"
     gene            complement(1929..2207)
                     /gene="traD"
                     /locus_tag="JMX01_RS26775"
     CDS             complement(1929..2207)
                     /gene="traD"
                     /locus_tag="JMX01_RS26775"
                     /inference="COORDINATES: protein motif:HMM:NF018152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_099745350.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="traD"
     gene            complement(2197..2628)
                     /locus_tag="JMX01_RS26780"
     CDS             complement(2197..2628)
                     /locus_tag="JMX01_RS26780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157769352.1"
                     /translation="MNKKQALWIGGKMNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNE
                     DVFNRCLKLYYAIDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNY
                     INIYELKKLFLLQKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     Typing          2979..4063
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 aatcgttaaa ggtgaagtct gtgccggttc cgggttctcg taagcgtcga gttgtcgagg
       61 aagacagcag tgacaacggt tagctaacca gcacagccag atcctacata agattacagg
      121 gcttcgctca tagcctacgg cctgtaccta tcaggctata gcttaacttg tgatgatttg
      181 aagccttcgg cctgtgcttc gcaaaaatac ccccagaccc attattacct gatatggcta
      241 aaatgaccac ttgacacttt tttagtggtg actacttgac accatgaaag gttatgttag
      301 aacagtaata taaaaacaaa aatccctgat ttccgaatgt ttggcgacag gagaaaatca
      361 gggatctaca ataaaaggat tgtattaaaa aatgatacta caaaaagaat atagcagaag
      421 tgattttata aatcaaatta tatcaagacc attttgcact gacgacttca aaagagatgg
      481 tatttataga acaaacaaaa gacgagcttt aaacagcgtt tatatcgagc ataacaacga
      541 tagcttcatt aactctattg tgtttgatat tgacagcgat acagcggcta ttgcatggca
      601 agatgcagat ataccgaagc ctaactttat aactcaaaat ccggctaatg gtcacgctca
      661 tttattttat gccttatcat ctcctgtttg tataacagaa aacgccagaa gaaagccaca
      721 aaagttatta aaaggcgtta tagaaggact caccgaaaga ttaggtgcag atccttgtta
      781 tacgggcaag ataacaaaaa accctctcaa tccgagatgg cgtacatttt ggaacgaaca
      841 accaagattt gaattgaact atctatgtga gttcatagat acaaataaaa gagtaaaaaa
      901 agaagtaaga aaatcaatag ttgcagaggg aaggaataca gcgttatttg atagtctgcg
      961 tttttacgct tacagcataa tttttaaata tcaaaaaaat gatgatttct gcggtttcat
     1021 ttcagcatta caagaagaag ctgaaaacat aaacgattca ttcgaagatc aactaagctt
     1081 taaagaaata aaccacacaa taagaagcat ttcatcttgg gtatggggga cttttgatag
     1141 tgatactttc agcgaaatac aaagcaacag agcaaagaaa gcgaaaggta aaactaaaac
     1201 caatacaaaa gcaaaaaaac ttatagaatt tataggagta aaaaaatgaa aggcaacgag
     1261 ataagaaagg gcttaacaca aaaagagatg gctaaaaagt tcaatctttc tttaagcact
     1321 gtaaaaaaat acacttctca accaagagaa cattatgaat cagaagcaga aaacagaaga
     1381 aaaacagctt atacgttaca ccataaaaaa ggcttaaaat ggcgtgaagt agctgaaaaa
     1441 atgaatacaa cagaaaatgc cgttaaagca ttagccaaaa gatacaaaca aatcgaccaa
     1501 gaaaacaggg gctaaaatgc cccttagctt tatttatatt caatcttgaa atcttcagac
     1561 tttacgaaat caattaatgc ttcaatatct tttggttttc ctgccttttc ataaacagct
     1621 tttgcaagaa taattaattt ttcgtctctt gctcgttgtc gttcttctct ttctgctttt
     1681 cttagttcgc taatctgctt ttttaaatcg tctatttttt cagctctaga tctgtgttta
     1741 cgaagtggcc ctgcagttgg tttttctgct gttttttgct cttttttttg agacattttt
     1801 tactctcccg gatgatatgt tttttaaaca tatcacaaag ccgggctacg gtgaagacta
     1861 acgcacaagc cccgccagcg gcgggttctt gcttgttcaa cccttccaag tgtcagatta
     1921 gaaatctgtc acttgtcagc gttttctaga cgtgatttta gcaatctttc accattgttt
     1981 tttaacgctt gtttttgctc atcgcttaac ttgttaaagt gttcaagaaa acccactaca
     2041 agagctagat cgtaatctaa accaaacacc cttgtaactt caccgccaac agtaaatttt
     2101 ttatgttctc tagcttttct ttctttctgc gatgcttctt ttttaagttt agataatcta
     2161 tcttgtgcaa gtgccattct ttgctctaac gtttttttac ttcccatact caacctctct
     2221 tttaattttt tctatctctg aaatcatact tttaaaatca aaatcattga tatttttttg
     2281 taataaaaac aactttttca gttcatagat atttatgtaa ttatttagtg ttaaatacac
     2341 aagatcataa tcagttataa ttggttcatc atgatcttta ttttcacgat ctttatttat
     2401 caaatatatc aacgcttcaa acaatgattt tttattgtta tagattgcat catcgatagc
     2461 gtaatacagc tttaaacacc tattaaaaac atcttcatta acttcgtcat tgatgaaatc
     2521 gtttttaata tcttcaatgt cgatctcgct tgtaaaaatc aatagccttt tttctatttc
     2581 ttttttattc attttaccac caatccataa tgcttgtttt ttattcaata tcgtaacttt
     2641 taaatattgt caaatttgac tttaatttaa tcagagttaa tttatatata tcagtgaaag
     2701 aaaagctagt ttgtttttca ttcctctttt agtttgagcg ttagcgaaaa ataaaagcta
     2761 aggaatgccc ggttacgcat tgtaaacaat gccggggtct gccgacggct aaaacctgtt
     2821 tttcttacga aaaactaaaa cccaaaccca aaagaaaaaa taggggttct catgaggata
     2881 taattatatg gctttatata gattagaaat gcagaacgtt agtcgttcgg atggtgtcag
     2941 ctcagttgca aaagctgctt atcgtcatcg ttccgtcatg atagatgaac gaaccggaga
     3001 aatacacggc gagaaatcag ctaatcgtga cgatctggtt tatgctgaaa tattggcacc
     3061 agaaggaacg ccagattttt tgattaaatc aagcaatgat ctatggaact atgttgaaaa
     3121 aacagagaag agaaaggacg caagaacagc aaaagaattt aaaataactt tgccttgtga
     3181 acttaccaat gaacaaaata taaaactatt gaaagaatac ttaacaaaaa actttgttag
     3241 taaaggcatc atttgcgact ttgttataca taacgacaaa gacaataaaa acccgcacgc
     3301 tcatgtgatg attacgacaa gagaaataac gctttcagga tttggtaaaa aagtaaggtc
     3361 atgggatgaa gaaaaaaccc ttcatgattg gcgtaaacaa tgggcaaaag ttcaaaatca
     3421 gcatttgaaa aatgcaggct taaaatctcg cgtcagtcat agaacactag aagaacaaaa
     3481 aaatataatg atcgatttag ctaaaaaagc agaagatgca aaagattttg acaagtctat
     3541 tttatatcta gcaaaagcta ttgaactaga tcgcccacca atgcagaact tatcacgtaa
     3601 aaaatggaga acaaaagaag gtcaagaaca acgcaaacgt gaccaagcta tcagagacag
     3661 ggcgagagcc gacgctaggg ctttcaggtt gttgagaggt gttgcagttg ttgatgttgc
     3721 ttcattcgtg gttcatccgt tagagaaaaa agagcagtca aaaaataatg acttttcaga
     3781 aaaaaaatct tggtggcgtt ctactgttaa ttttataaaa aatattaaag ctaagttctc
     3841 aactaaaaaa gatattaaaa acgatgtcca tgcatcagac gatttaatga ctgatccggt
     3901 gacgggttta ccaatcacca aagcgaaatg gcaagagatg agtgacaggg caaacgtaag
     3961 gactccacag cctgcgatct catatcaggt taagcaatcg gacggggaag ggctaaaaac
     4021 ggctcagaat gcgttacagg acgttttaga cgtatcagag ggta
//
