LOCUS       NZ_LR890469             4074 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB1_7A isolate MSB1_7A plasmid 4, complete
            sequence.
ACCESSION   NZ_LR890469
VERSION     NZ_LR890469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357463
            Assembly: GCF_904864615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:33:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,163
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,839
            CDSs (with protein)               :: 4,839
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 77 of 200
            Pseudo Genes (incomplete)         :: 150 of 200
            Pseudo Genes (internal stop)      :: 38 of 200
            Pseudo Genes (multiple problems)  :: 56 of 200
            Pseudo Genes (short protein)      :: 3 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890469.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB1_7A"
                     /isolate="MSB1_7A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            join(3447..4074,1..1532)
                     /gene="mobQ"
                     /locus_tag="JMW16_RS25505"
     CDS             join(3447..4074,1..1532)
                     /gene="mobQ"
                     /locus_tag="JMW16_RS25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1790..2482)
                     /locus_tag="JMW16_RS25510"
     CDS             complement(1790..2482)
                     /locus_tag="JMW16_RS25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3014..3268)
                     /gene="mobC"
                     /locus_tag="JMW16_RS25515"
     CDS             complement(3014..3268)
                     /gene="mobC"
                     /locus_tag="JMW16_RS25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(3270..3446)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3447..3805
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(1791..2482)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1529
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.607"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3361..4073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.739"
                     /coverage="99.0"
                     /evalue="1.54e-155"
ORIGIN
        1 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacaggctta
       61 acctggaaat acagatttcc cgcactgaac tcaggaacag cgggctgaca gagcctgccc
      121 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
      181 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
      241 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
      301 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
      361 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
      421 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
      481 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
      541 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
      601 agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc aggaaaaatg
      661 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
      721 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
      781 acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag cgtgttgatt
      841 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa catccaccac
      901 tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt tgccagacat
      961 ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     1021 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     1081 gacgtctgcc accgacgctg atgccggtaa tgagttctgt cgaccgggaa acccgccagc
     1141 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     1201 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     1261 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     1321 acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc cgtgaggcac
     1381 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     1441 gtttcaatcg tacctgggac gaatggcggc gcgaggaaga tgcaagaaca cacaccaccg
     1501 agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtttaac
     1561 atcgtgttat gttcgtcgat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     1621 cactcacata atgcaagcat agttttgttt ttccgccccc aaaaattggg ggttcttttt
     1681 tcgctgcatc agagcgtttt tgacgcaatt tttcacttca atagtacctt cgcactacta
     1741 acacaaaaaa aacgcagcag ggagcgtttt aggtgcctta aaatcgattt tagatcgtca
     1801 tcttgatctg tttgtcttcc ttgaagcgaa aatgcagggc tacaaccgtg cgcccctttt
     1861 tgatcgtctc aaccgttaca gccaggtcgc tttttttgtt cagttcgtca acacagggtt
     1921 ttattagtat tttatttaag tctctgaatt gtttgtattt attttcaatc cctaacattg
     1981 aacgaaagtc attaagagca ataattctct ctcccgtact gcggaattgc tggaggaact
     2041 cgtatattct tatactgtaa ctacttgata aacctgatat gttttttata acaacgcgcg
     2101 tgaattgccc cttcagttgc gtcagataag gcattaccgc gtcagaaaag gttattcgag
     2161 ccttggcctc acctttagcg tactgtgccc gatactggat ccagcggaac tcctcacgct
     2221 tctcgtcatt cttgagaatg atcgacctgt cccataaacg atcgatcgct tcctgtaact
     2281 gaacctcggc atttttccgc cccatgtcag ggaatgaatc aaaatattcc gcagcggtaa
     2341 tggtcattgt cttttgcaat tcagcatcag caccagattt caatcgactg atacacagca
     2401 acagaaaacg ttgttcctgt agggtcaact tgtagctggc ctcaaccaaa gcattcgact
     2461 tgtaggcaat caagtccgac atacaaagat cccgttcaaa tgtggtgatg ctttaatgtg
     2521 ccccacaaat aagaaatgtg gaatgttttt ttttatatcc tcacatttct gtgaataaca
     2581 ccttaaattc ctccaccttt accttaaatt cctccaccta aacaccttaa attcctccac
     2641 ctttacctta aattcctcca catataccac tataacatat tgattttatt aatgaatttc
     2701 acccctaaaa caagtattaa aaacaagatc ttaaaaacaa gatctaaaaa cttaacggcg
     2761 agcctgtgga taacttgggg tgctgaaaac aaaaaaccaa taaaaacaat gtgttgcaag
     2821 aaagcagaat tgcaagttcc gaatactccc cttccaaccc tgtggaaatc cagtattcat
     2881 gcgagttcag cgcatatcac ctgccgaaaa accgtgcgta tgtgctcaac ctggcgacgg
     2941 attcacttcc gcttcctcgc ccactcgctc gctaacgctc ggtcgctggg gctgcggcga
     3001 gcggacgata caatcactgt cctggcagcg cctccagctc gttaatcagc ggcagtatgc
     3061 gcgtcacatc agcctgtcgt gtcacctctg ccttcaggaa ctggagtaac caggcacgca
     3121 actgcacctc ctccggcttt ctgacctttg ccagcaatgc tgcacccaca acaaccttct
     3181 gcgctgtgtc caggctccgg ctcttcgcct tcaggcgctg taatctcgcc tcagcatcag
     3241 caatcttctg ttcgagcgtt ctgctcatga tgtaactccg tgcgcgatga aaaatcgcat
     3301 tttagcgcgt cactggtagt ttaaaaactg agctggcata atgcacggca aatcacgaag
     3361 tgcgcactta tacaatctcc actccgtttc gattgtgtgc gccctgcggg gctgaaagaa
     3421 aactacaaaa aggcattacg gcagaaatgg cgatttatca tctcagcatg aaaattatct
     3481 ccagaagcag cggctacagc gccgtagctt ccgctgccta tcgttccggt tcactaatgc
     3541 ttgatgagcg taccggactg actcacgact acacccgcaa aagcggtgtg gctgaagctg
     3601 tgattctgac ccccgcgaca gcccctgcct ggtgtacgaa ccgtgccgaa ctctggaatg
     3661 ccgttgagaa ggccgaacgc cggaagaact cacaacttgc gcgggaaatt gaactcgcta
     3721 tcccccgtga actgccgcag gatgccgcac gggaaaccgt tctcgccttt gtcagagaaa
     3781 actttgtcag tcagggcatg attgctgatg tggcgttcca tcacatggat aaaacaaatc
     3841 cccatgcgca catcatgctg accacgaggg ctgtcggacc tgctgggttc ggcggaaagg
     3901 tcagggactg gaacgaccgg acacatgccg agacgtggcg tgcatcatgg gctgaccatg
     3961 caaacagagc actggcgaac gccggttatc aggaagagat agaccatcgt tcatacgagc
     4021 gacaggggct tgagaaaacg cctggtattc acctcggaaa gtctgcctgt gcga
//
