LOCUS       NZ_LR890414             2113 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MSB2_1A isolate MSB2_1A plasmid 5, complete
            sequence.
ACCESSION   NZ_LR890414
VERSION     NZ_LR890414.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357497
            Assembly: GCF_904864635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:09:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,870
            CDSs (total)                      :: 4,753
            Genes (coding)                    :: 4,551
            CDSs (with protein)               :: 4,551
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 74 of 202
            Pseudo Genes (incomplete)         :: 136 of 202
            Pseudo Genes (internal stop)      :: 38 of 202
            Pseudo Genes (multiple problems)  :: 41 of 202
            Pseudo Genes (short protein)      :: 4 of 202
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890414.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MSB2_1A"
                     /isolate="MSB2_1A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="5"
                     /geo_loc_name="Australia"
                     /collection_date="2014-04"
     gene            16..981
                     /locus_tag="JMW04_RS24030"
     CDS             16..981
                     /locus_tag="JMW04_RS24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            985..1251
                     /locus_tag="JMW04_RS24035"
     CDS             985..1251
                     /locus_tag="JMW04_RS24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          410..641
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atcggagcag gcttaatgtc agaagataaa ttcctttcgg actacagccc ccgtgatgca
       61 gtttgggata cccagcgcac gcttaccgat tctgtcgggg gtatctacca gactgctgct
      121 gaattcgagc gctatgcact ccgtatggcc tcctgtagcg gtttgttacg ttttggttgg
      181 tctaccatca tggaaaccgg agaaacgcgc ctacggcttc gtagtgcgca attttgccgt
      241 gtccgtcatt gccctgtctg ccagtggaga agaaccctca tgtggcaagc ccgtttttat
      301 caggctctac cgaaaatcgt tgtggattac ccgtcttccc gatggttgtt tctgacgtta
      361 actgtcagga actgcgagat aggtgaactt ggaacagtcc ttacagcaat gaatgcggcg
      421 tttaagcgaa tggaaaagcg aaaggagcta tcacctgttc aggggtggat cagggctacg
      481 gaggtgacgc gaggtaagga tggcagcgca catccgcatt ttcactgtct gctgatggtg
      541 caaccttctt ggtttaaagg gaagaactac gttaagcacg aacgttgggt agaactctgg
      601 cgcgattgct tgcgggtgaa ctatgagccg aatatcgata ttcgggcagt aaaaactaag
      661 acaggtgagg ttgtggccaa cgttgccgag caactgcaaa gcgcggttgc tgaaacgctg
      721 aaatactccg ttaaaccgga agatatggca aacgatcctg agtggtttct tgagctgacg
      781 cggcagcttc acaagcgccg ttttatctcg accggtgggg cgctaaaaaa cgtcctccag
      841 ttggatcgag aaaccaatga ggatcttgtc attgccgacg atgtagggga tggcactgat
      901 gacgggaagc ggacggcgtt tgtctgggat tcaggtaaac ggcgttacaa acgcgcccct
      961 gagaaggata aatcggatta acgtatgaat attaatattg aatacctgaa tggaaataag
     1021 actattggtt tatttttttt aagaagtgaa gcggtgattc ctgacaggtt taaaaacctt
     1081 attttgctta ttgatggatt aagttttggc acatttggtt ttcatccgca cgaaggtttt
     1141 gaggatgaat taattttata tattcagaaa acaaacgaga gggtaaaaac tctttttgtg
     1201 aaaatagatt taaacggtat caagtttgga tttttaagaa cgcattctta gttctggaaa
     1261 agagccagcg gcaggctgag gtgataggta cgagattgca tgcaatctct agtgctctgt
     1321 ctatcctgca ttatcctcag cattatcctc agcattatcc tcagcattat cctcagcctt
     1381 gccaactcga caccaatgca ggatagacaa tccgatgtca aatgttaaca ctctgcgagt
     1441 ggtacatttt ccccggatta tcgtcctgag cctgccgctg gctctctttc taccgcctcg
     1501 ctttgctcgt tgctcaacgc ctcacagaca cggattaaaa tccgcatccg ttcaccgttt
     1561 tttaaagtcc gttaaaagca tgatgccatc tccgagagtt aatctcgtca aatgctaaat
     1621 cgtgggggtc ccctttgggg ttccgattta gtgattgacg acaccaccga ttaaaaaact
     1681 tatgcggggt ggatggtttc acgaagtgag gccatccacc tgtaagacag ggttttgttt
     1741 ttattccctg ttttggtgat cgggtgtgtg gaaaaggttg gggtaagccg ttcgggggtg
     1801 cttgttttgg ggggttaaaa ttgtggttat tttttgcgca attctcgcgc gtgatccttg
     1861 tatttatact taagggataa atggcggata tgaaatagtg gtttagccca gtaatgacga
     1921 ggctttgagt gggttttgac aggtcaaaga aaatggagca gaattgaggc gtttttaatc
     1981 ggcgttgggg agtgcgtcaa cactccccaa catttcgaat gtgtcacctc agcggcaaac
     2041 tctggtgaca tgtactggct cgcaatgcac aggtacgtga tgaatatacc acatcaaatc
     2101 acagcctgcc cag
//
