LOCUS       NZ_LR890377             4228 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF250 isolate INF250 plasmid 4,
            complete sequence.
ACCESSION   NZ_LR890377
VERSION     NZ_LR890377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357225
            Assembly: GCF_904863395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:24:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,575
            CDSs (total)                      :: 5,452
            Genes (coding)                    :: 5,303
            CDSs (with protein)               :: 5,303
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 60 of 149
            Pseudo Genes (incomplete)         :: 87 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 23 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF250"
                     /isolate="INF250"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2013-12"
     gene            join(3724..4228,1..202)
                     /locus_tag="JMW58_RS27660"
     CDS             join(3724..3973,3973..4228,1..202)
                     /locus_tag="JMW58_RS27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_227498728.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLARLGRKSLSFSKSVELHD
                     KVIGHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(<485..565)
                     /locus_tag="JMW58_RS28015"
                     /pseudo=""
     CDS             complement(<485..565)
                     /locus_tag="JMW58_RS28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            999..1190
                     /locus_tag="JMW58_RS27665"
     CDS             999..1190
                     /locus_tag="JMW58_RS27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_049139654.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEELHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1190..1591
                     /locus_tag="JMW58_RS27670"
     CDS             1190..1591
                     /locus_tag="JMW58_RS27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048270303.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNILLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2442..2612
                     /locus_tag="JMW58_RS27675"
     CDS             2442..2612
                     /locus_tag="JMW58_RS27675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771094.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHP"
                     /name="hypothetical protein"
     gene            complement(2635..3341)
                     /locus_tag="JMW58_RS27680"
     CDS             complement(join(2635..3092,3092..3341))
                     /locus_tag="JMW58_RS27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_227498728.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLARLGRKSLSFSKSVELHD
                     KVIGHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          complement(439..823)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.487"
                     /coverage="89.0"
                     /evalue="2.97e-154"
ORIGIN
        1 gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg
       61 aattgagcgg cataacctga atctgaggca gcacctggca cggctggcac ggctgggacg
      121 gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct
      181 gaacataaaa cactatcaat aagttggagt cattacccat ttgttcatca aatgccatga
      241 tcttcttgag attcattcgc tcaaagcgta atctctttct cggaaaacga aaaaaccacc
      301 tggggaggtg gtttttcgaa ggttcagtaa gttggggaac ttctgaaccg tggtaacagt
      361 cttgtgcgag acatgtcacc aaatctgtcc tttcagtgta gccgcagtca ggccaccact
      421 tcaagaactc tcgatacatc tctcgcacat cctgtctacc agtggccgtt gtcaatggcg
      481 attagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      601 tgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      661 acaggtatct ggtaagcggc agggtcggaa caggagagcg cacgagggag ccatcagggg
      721 gaaacgcctg atatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcagt
      781 ttctgtgatg cttgtcaggg ggggcggagc ctatggaaaa acggccgctg tgcggcctct
      841 ccttctcccc atcacgcgat cgctcggttt tctcgagctt ttatacgcat gctcctgccg
      901 ctcgccgcag ccgaacggca ggagcgtagc gactgagtga gcgaggaagc ggaaaagaga
      961 acatccctac attgagcact gacgctctga ggcctcttat gacgaaacaa cagaaaacag
     1021 cgctgaacat ggctaaattc atccagaatc agtctctgct cctgctggag aagcttaacg
     1081 agcttgatct cgatgtcgag gccgaccttt gtgaggaact ccacgatgat gcggagcatc
     1141 ttttccgtac actgtcctcg agactggatg cattacagga tggtaactga tggccagagt
     1201 gtctattagt gaggctgccc gactgacggg taagagtaga acaacactgc acaggttaat
     1261 taaaacaggt gacctgtcca cctgtagtgg tgagcgcaat gcaaaaatgg tagatacttc
     1321 agagctttta cgtgtctttg gtccctttga gcaaccgaag ggtgaacacc attctggaca
     1381 ggtctccgaa cagcgtgata cagcctctgc tgaccagagt gaacaggtga ttcgtcatct
     1441 gaaacaggag attgaacacc tgaatatctt acttttgtcc aaggattcgc acattgacag
     1501 cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca aaattaactg aatccccggc
     1561 gccatggtgg aaattctgga aaaaatcctg accccgtcat gcttccgttt tcctctccgg
     1621 ccatcccggg gagttcctga accttccaaa aaatcgccgg atttctgaac gctcctcacc
     1681 actaaaaata tccacacact acgcaaaatt aaaattttgc ggatttgtat ttaatatcag
     1741 tagtttaagt ctgtaaaatt tcgtttttta aatttgattt atatagatcc gactctatga
     1801 cgactcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc aggactataa
     1861 aaacaatgag ttaataacga cccgactcta gacccgactc cccgtttggt tgttttttag
     1921 gcgtggcgac tctgggtacg actctagtga agagtcgccg gtgaatcgtg tggtgagtcg
     1981 ttcggtgact tgccataacg tgattgccgg ccgtcgacga accggattcc tgaacgtgct
     2041 gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc
     2101 cgttcaccgt tttcggatgg gaagtacgcc ggaaacagga cttaccctgg ctgaatggcg
     2161 tgaattttcg cgctatgcac ttgcgtgcat actcatgcat gccgtaaaaa cagagcctgc
     2221 gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac
     2281 gccctgaggc cgttttagcg gtgcgtacaa tgggacgtta tggtaaatgc ctgtcttgct
     2341 cacttttaga gcatgaggca aaaaaggtaa aaacttattt gcttaacagc ccctatcact
     2401 atgtaaatta actcctgaaa actaaaaaat ggaggattgg catggcgact gctactaatt
     2461 ttgatgcgtg gttagatgat gttgatggcg attatgagga ggttatggct ctttatgatt
     2521 ccgttcagaa tgtaagtgat atggggttgt atcagtgtgt tgagggtggt cggggtgatg
     2581 cttgggtcgt gagctctaat catcatccat aagctctttg gtaatgactc caacttattg
     2641 atagtgtttt atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga
     2701 gaacgacagc gacttccgtc ccagccgtgc cagccgtgcc aggtgctgcc tcagattcag
     2761 gttatgccgc tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag
     2821 gcgggattca tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag
     2881 caggctcata agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac
     2941 cgtcttccgg agcctgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac
     3001 gtatccccac tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg
     3061 cgaggttacc gactgcggcc tgagtttttt aaatggcgga aaatcgtgtt gaggccaacg
     3121 cccataatgc gggcggttgc ccggcatcca acgccattca tggccatatc aatgattttc
     3181 tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca gttgccatgt tttacggcag
     3241 tgagagcaga gatagcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca
     3301 gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac
     3361 catcatacac taaatcagta agttggcagc atcacctttt tttgtatgta caaactcaag
     3421 gatttaggaa taatctcaat ccccttaaca agcacatgca aaaaaaacca tgacgatgct
     3481 tcatctcaaa acaataagta acgaaagtat catgctaaaa aaagatgata ttatcctaat
     3541 aaaatcatat ggtaacgaaa aaacaaaaat atttttacgg ggtgtgaatg aggttctaat
     3601 gattgacgcg agctttgaag aggtaaagtt tgctatcaat aatgaccttc aggatcttca
     3661 ggatcttcgg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     3721 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     3781 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
     3841 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
     3901 gccatgaatg gcgttggatg ccgggcaacc gcccgcatta tgggcgttgg cctcaacacg
     3961 attttccgcc atttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
     4021 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg atacgtcggg gctaaatcgc
     4081 gccagcgctg gctgttttac gcgtatgaca ggctccggaa gacggttgtt gcgcacgtat
     4141 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
     4201 tggtgatatg gatgacggat ggctggcc
//
