LOCUS       NZ_LR890347             3223 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB2_2B isolate KSB2_2B plasmid 5,
            complete sequence.
ACCESSION   NZ_LR890347
VERSION     NZ_LR890347.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357408
            Assembly: GCF_904863295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:21:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,272
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 190 of 280
            Pseudo Genes (incomplete)         :: 102 of 280
            Pseudo Genes (internal stop)      :: 24 of 280
            Pseudo Genes (multiple problems)  :: 33 of 280
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890347.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_2B"
                     /isolate="KSB2_2B"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="5"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            complement(join(3058..3223,1..158))
                     /locus_tag="JMW06_RS26770"
     CDS             complement(join(3058..3223,1..158))
                     /locus_tag="JMW06_RS26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1176..1508)
                     /locus_tag="JMW06_RS26775"
     CDS             complement(1176..1508)
                     /locus_tag="JMW06_RS26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1676..1891)
                     /locus_tag="JMW06_RS26780"
     CDS             complement(1676..1891)
                     /locus_tag="JMW06_RS26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1895..2380)
                     /locus_tag="JMW06_RS26785"
     CDS             complement(1895..2380)
                     /locus_tag="JMW06_RS26785"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2415..2849)
                     /locus_tag="JMW06_RS27195"
                     /pseudo=""
     CDS             complement(<2415..2849)
                     /locus_tag="JMW06_RS27195"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             complement(1177..1508)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(255..446)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          426..909
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(4..158)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.154"
                     /coverage="99.0"
                     /evalue="1.02e-26"
     Typing          complement(1579..3068)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctgacgaa gcagcgccgg cgagatcgac ggaagtttgc cggcgcgtgc gggcttctcg
       61 tccaggcacg tctgccgcat ccaggccgcg agttgcttac cctcgcagcg ctccagcaga
      121 cgccggtgct cgtcctcagt cacccacatg gtgagcattt tgctgcgttt gtctgccaat
      181 ccggcctcct gataacgccg ggacggacgg gaaatctttt cccggtcggc acggtgttgg
      241 acaagccgca ggcgcggcag tggttttagc gggtttcggg gcgcagccct gaaccagtca
      301 cgtagcgcta gcggagtgta tactggctaa acatgttatg acggagtggc aatttatgaa
      361 agtgcttcat gtgacagggg aaaaatgcgg ctgccgggcg taagtgcaaa atgcaggtgg
      421 tgatgaaatg acgcctcctc gctcactcgg tcgctacgct cctgccgtga gactgcggcg
      481 ggcgttacca gctcacagat tacgggataa gcaaatgccg ctcgaagcag gaaaggccaa
      541 gaaaaggatg cggagttgcc gtttttccat aggctccgcc cccctgacga gtatcacgaa
      601 aatgacgctc aaatcagcgg tggcgaaacc cgatgcggct taaagatacc aggcgtttcc
      661 ccctggtggc tccctcgtgc tcccccgcat tcctgccttt cggtttaccg gtgtcattcc
      721 gctgttatgg ccgcggtttc tcattccacg cctgaaactc ggttccgggt aggcagttcg
      781 ctccaggatg gactgtatgc acgaaccccc cgttcagtcc gactaccacg cccgttccgg
      841 taactatcaa cttgagtcca acccggaaag acacgacaaa tcgccagtgg cggtagccat
      901 tggtaactgg gttcacggag cagagattgt agagaatgcc tacagagttc ttgaagtgaa
      961 agcctgagtg cggctacact gaaaggacag tttaggtatc tgcgctccgt gaaagccagt
     1021 taccatcgga acaaagagtt ggtagctctg aattccggca aacgaaccac cgttggtagc
     1081 ggtggttttt ttgtttacag ggaaaaagat tacgcgcaga aagaaaggat ctcaaggaaa
     1141 tccttttgct ttggccaata tgcttcggta aaaagttaat gcgcagaaga ttttgaaaaa
     1201 atattaataa ccaaaactcc aacgcaaata aggaacattc ctgcaattgc gggccaatca
     1261 agtttttgac cgctaaccaa ccaaccagct aggcttatta gtacaattcc cgcaccagac
     1321 caaattgcgt aagcaatacc ggtaggtata ttttccaatg tttgggaaag cagataaaag
     1381 gcagcgcaat aacttattgc agttcctaca gacggccaca ggcgtgtaaa tccttctgaa
     1441 tatttcataa aggtggtgcc tatgacttca gccacaatag cccctcccag gaaaatatat
     1501 gtattcataa aaatacctca tgttgaaaaa tttaaatatc tttacataac aactaattaa
     1561 tagaacccgg cttatcatag cgaaggacca tgatgctgcc actgcgatcg ttccagctcc
     1621 agcgctttct ggtgctgcac cgcccgctcc tgctgccggt gagtttctat cgcgttcagc
     1681 ctttcgacaa ccggcgcagc tgctcgctca aactctgcac ctgcctgctc aagaccgtga
     1741 cgcgctcgtt cagcgccgcg ttctcccgtt gaataagacc agacatcgtc cgccattccg
     1801 cgaaggcgtt ctcccactcg tccagccttt tcgagtagtc ctgctgtagc tgctctaatg
     1861 cgctcagcaa ctgtttttcc agctccgtca tgtgctattt ccccgccagt atcatccagc
     1921 tcgagtcctc tccgaactgt cccgcttccg ggttcaccct cacgcagcgg cgtctctgct
     1981 cgccgcaggt cgagagctgt acgccgctgt tcctctctga cagcatggcc tgcgtgctct
     2041 tctgctcccg gatggtcgta tagttctcga gtatctgctg cccctgccac cacagaatgc
     2101 ccgcgctcga cgctatcagc agcgcggaga ccagaacgat ggtcagccac gtctggctga
     2161 ccatgcgcag catccctttc gtgcgctggc tcatggctga ggacagcttc cggtcgtggt
     2221 cgaggatggc ggcgctgatt ctcttctcgc tttcgctcag ttccgctctg acagacttct
     2281 cgtgctcgct gaatgcggct ttcagcatct cgccggtagt ctgctgctgc gctttcgatt
     2341 tctgctctaa gtccttcgcc agcgttaaaa gactgttcat agatggctcc cttcagtcgg
     2401 atgtttcgcc ccccgtccgg gtcggcaatg ctgatgctgc tttttgtggt gcgcaccacc
     2461 tcaaaacctg cgctttccag cgcctcagtg acgtcctgac gcgttttcag ctccccggat
     2521 gaggcaaggg caagcaggcc gctcgtaatc gcctgggcgg cttcctgctt cgcttccggc
     2581 agcgcggaag gcgagaccag cacccgccgg ttctccggcg cgttcgggtc atgcagcccc
     2641 agcctgccgt tcactatggt ctgccaggca tcgatgcgcg gacggtcggc gcggtcgtaa
     2701 tacggctgta gccgcttgcc ggtcagaagc tccgtgttcg ggatcaggaa gttcagctcc
     2761 agccgcccct tgtcccggtg ctcaacccac agcacgctgt actggtcttt atcgagtccg
     2821 ggcatcagaa cccgctcgaa actcgccatc agcttttcgc gctggccggg cggtaaatcc
     2881 tgttcggcaa aagacagaac gccggaagtg tactttttgg cgtagggcga ggcgtcgata
     2941 agctcccgga cttcatccgg cttcccctgc agaacgctgg cgccgtcgcg ctggcgatct
     3001 ttacccagca gataatcgac cggcccgccg ccgccgccgc gcccccgagg atgaaactta
     3061 acgatcatca tcagcaccct tttccagtac ggcatgccgc aaccgctcga gtccggcatc
     3121 gatggccatc agcgcggcga caatctgcac gcggtcgtgt ccgcttccac caccggcgtt
     3181 aacctggcgg gcaatctggt taaggttgtt ccccatgccg gca
//
