LOCUS       NZ_LR890346             4064 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB2_2B isolate KSB2_2B plasmid 4,
            complete sequence.
ACCESSION   NZ_LR890346
VERSION     NZ_LR890346.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357408
            Assembly: GCF_904863295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:21:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,272
            Genes (coding)                    :: 4,992
            CDSs (with protein)               :: 4,992
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 190 of 280
            Pseudo Genes (incomplete)         :: 102 of 280
            Pseudo Genes (internal stop)      :: 24 of 280
            Pseudo Genes (multiple problems)  :: 33 of 280
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890346.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_2B"
                     /isolate="KSB2_2B"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
                     /geo_loc_name="Australia"
                     /collection_date="2014-03"
     gene            join(3686..4064,1..881)
                     /gene="mobQ"
                     /locus_tag="JMW06_RS26740"
     CDS             join(3686..4064,1..881)
                     /gene="mobQ"
                     /locus_tag="JMW06_RS26740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_099745348.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="mobQ"
     gene            1190..2047
                     /locus_tag="JMW06_RS26745"
     CDS             1190..2047
                     /locus_tag="JMW06_RS26745"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_099745352.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication initiation protein"
     gene            2044..2313
                     /locus_tag="JMW06_RS26750"
     CDS             2044..2313
                     /locus_tag="JMW06_RS26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_099745351.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(2328..2594)
                     /locus_tag="JMW06_RS26755"
     CDS             complement(2328..2594)
                     /locus_tag="JMW06_RS26755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519627.1"
                     /translation="MSQKKEQKTAEKPTAGPLRKHRSRAEKIDDLKKQISELRKAEREE
                     RQRARDEKLIILAKAVYEKAGKPKDIEALIDFVKSEDFKIEYK"
                     /name="hypothetical protein"
     gene            complement(2727..3005)
                     /gene="traD"
                     /locus_tag="JMW06_RS26760"
     CDS             complement(2727..3005)
                     /gene="traD"
                     /locus_tag="JMW06_RS26760"
                     /inference="COORDINATES: protein motif:HMM:NF018152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_099745350.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="traD"
     gene            complement(2995..3426)
                     /locus_tag="JMW06_RS26765"
     CDS             complement(2995..3426)
                     /locus_tag="JMW06_RS26765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157769352.1"
                     /translation="MNKKQALWIGGKMNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNE
                     DVFNRCLKLYYAIDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNY
                     INIYELKKLFLLQKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     Typing          4..878
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.1e-158"
     Typing          3777..4063
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.1e-55"
ORIGIN
        1 tacataacga caaagacaat aaaaacccgc acgctcatgt gatgattacg acaagagaaa
       61 taacgctttc aggatttggt aaaaaagtaa ggtcatggga tgaagaaaaa acccttcatg
      121 attggcgtaa acaatgggca aaagttcaaa atcagcattt gaaaaatgca ggcttaaaat
      181 ctcgcgtcag tcatagaaca ctagaagaac aaaaaaatat aatgatcgat ttagctaaaa
      241 aagcagaaga tgcaaaagat tttgacaagt ctattttata tctagcaaaa gctattgaac
      301 tagatcgccc accaatgcag aacttatcac gtaaaaaatg gagaacaaaa gaaggtcaag
      361 aacaacgcaa acgtgaccaa gctatcagag acagggcgag agccgacgct agggctttca
      421 ggttgttgag aggtgttgca gttgttgatg ttgcttcatt cgtggttcat ccgttagaga
      481 aaaaagagca gtcaaaaaat aatgactttt cagaaaaaaa atcttggtgg cgttctactg
      541 ttaattttat aaaaaatatt aaagctaagt tctcaactaa aaaagatatt aaaaacgatg
      601 tccatgcatc agacgattta atgactgatc cggtgacggg tttaccaatc accaaagcga
      661 aatggcaaga gatgagtgac agggcaaacg taaggactcc acagcctgcg atctcatatc
      721 aggttaagca atcggacggg gaagggctaa aaacggctca gaatgcgtta caggacgttt
      781 tagacgtatc agagggtaaa tcgttaaagg tgaagtctgt gccggttccg ggttctcgta
      841 agcgtcgagt tgtcgaggaa gacagcagtg acaacggtta gctaaccagc acagccagat
      901 cctacataag attacagggc ttcgctcata gcctacggcc tgtacctatc aggctatagc
      961 ttaacttgtg atgatttgaa gccttcggcc tgtgcttcgc aaaaataccc ccagacccat
     1021 tattacctga tatggctaaa atgaccactt gacacttttt tagtggtgac tacttgacac
     1081 catgaaaggt tatgttagaa cagtaatata aaaacaaaaa tccctgattt ccgaatgttt
     1141 ggcgacagga gaaaatcagg gatctacaat aaaaggattg tattaaaaaa tgatactaca
     1201 aaaagaatat agcagaagtg attttataaa tcaaattata tcaagaccat tttgcactga
     1261 cgacttcaaa agagatggta tttatagaac aaacaaaaga cgagctttaa acagcgttta
     1321 tatcgagcat aacaacgata gcttcattaa ctctattgtg tttgatattg acagcgatac
     1381 agcggctatt gcatggcaag atgcagatat accgaagcct aactttataa ctcaaaatcc
     1441 ggctaatggt cacgctcatt tattttatgc cttatcatct cctgtttgta taacagaaaa
     1501 cgccagaaga aagccacaaa agttattaaa aggcgttata gaaggactca ccgaaagatt
     1561 aggtgcagat ccttgttata cgggcaagat aacaaaaaac cctctcaatc cgagatggcg
     1621 tacattttgg aacgaacaac caagatttga attgaactat ctatgtgagt tcatagatac
     1681 aaataaaaga gtaaaaaaag aagtaagaaa atcaatagtt gcagagggaa ggaatacagc
     1741 gttatttgat agtctgcgtt tttacgctta cagcataatt tttaaatatc aaaaaaatga
     1801 tgatttctgc ggtttcattt cagcattaca agaagaagct gaaaacataa acgattcatt
     1861 cgaagatcaa ctaagcttta aagaaataaa ccacacaata agaagcattt catcttgggt
     1921 atgggggact tttgatagtg atactttcag cgaaatacaa agcaacagag caaagaaagc
     1981 gaaaggtaaa actaaaacca atacaaaagc aaaaaaactt atagaattta taggagtaaa
     2041 aaaatgaaag gcaacgagat aagaaagggc ttaacacaaa aagagatggc taaaaagttc
     2101 aatctttctt taagcactgt aaaaaaatac acttctcaac caagagaaca ttatgaatca
     2161 gaagcagaaa acagaagaaa aacagcttat acgttacacc ataaaaaagg cttaaaatgg
     2221 cgtgaagtag ctgaaaaaat gaatacaaca gaaaatgccg ttaaagcatt agccaaaaga
     2281 tacaaacaaa tcgaccaaga aaacaggggc taaaatgccc cttagcttta tttatattca
     2341 atcttgaaat cttcagactt tacgaaatca attaatgctt caatatcttt tggttttcct
     2401 gccttttcat aaacagcttt tgcaagaata attaattttt cgtctcttgc tcgttgtcgt
     2461 tcttctcttt ctgcttttct tagttcgcta atctgctttt ttaaatcgtc tattttttca
     2521 gctctagatc tgtgtttacg aagtggccct gcagttggtt tttctgctgt tttttgctct
     2581 tttttttgag acatttttta ctctcccgga tgatatgttt tttaaacata tcacaaagcc
     2641 gggctacggt gaagactaac gcacaagccc cgccagcggc gggttcttgc ttgttcaacc
     2701 cttccaagtg tcagattaga aatctgtcac ttgtcagcgt tttctagacg tgattttagc
     2761 aatctttcac cattgttttt taacgcttgt ttttgctcat cgcttaactt gttaaagtgt
     2821 tcaagaaaac ccactacaag agctagatcg taatctaaac caaacaccct tgtaacttca
     2881 ccgccaacag taaatttttt atgttctcta gcttttcttt ctttctgcga tgcttctttt
     2941 ttaagtttag ataatctatc ttgtgcaagt gccattcttt gctctaacgt ttttttactt
     3001 cccatactca acctctcttt taattttttc tatctctgaa atcatacttt taaaatcaaa
     3061 atcattgata tttttttgta ataaaaacaa ctttttcagt tcatagatat ttatgtaatt
     3121 atttagtgtt aaatacacaa gatcataatc agttataatt ggttcatcat gatctttatt
     3181 ttcacgatct ttatttatca aatatatcaa cgcttcaaac aatgattttt tattgttata
     3241 gattgcatca tcgatagcgt aatacagctt taaacaccta ttaaaaacat cttcattaac
     3301 ttcgtcattg atgaaatcgt ttttaatatc ttcaatgtcg atctcgcttg taaaaatcaa
     3361 tagccttttt tctatttctt ttttattcat tttaccacca atccataatg cttgtttttt
     3421 attcaatatc gtaactttta aatattgtca aatttgactt taatttaatc agagttaatt
     3481 tatatatatc agtgaaagaa aagctagttt gtttttcatt cctcttttag tttgagcgtt
     3541 agcgaaaaat aaaagctaag gaatgcccgg ttacgcattg taaacaatgc cggggtctgc
     3601 cgacggctaa aacctgtttt tcttacgaaa aactaaaacc caaacccaaa agaaaaaata
     3661 ggggttctca tgaggatata attatatggc tttatataga ttagaaatgc agaacgttag
     3721 tcgttcggat ggtgtcagct cagttgcaaa agctgcttat cgtcatcgtt ccgtcatgat
     3781 agatgaacga accggagaaa tacacggcga gaaatcagct aatcgtgacg atctggttta
     3841 tgctgaaata ttggcaccag aaggaacgcc agattttttg attaaatcaa gcaatgatct
     3901 atggaactat gttgaaaaaa cagagaagag aaaggacgca agaacagcaa aagaatttaa
     3961 aataactttg ccttgtgaac ttaccaatga acaaaatata aaactattga aagaatactt
     4021 aacaaaaaac tttgttagta aaggcatcat ttgcgacttt gtta
//
