LOCUS       NZ_LR890340             4062 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_9A isolate MINF_9A plasmid 7, complete
            sequence.
ACCESSION   NZ_LR890340
VERSION     NZ_LR890340.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357504
            Assembly: GCF_904863255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:13:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 5,054
            CDSs (with protein)               :: 5,054
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 73 of 200
            Pseudo Genes (incomplete)         :: 136 of 200
            Pseudo Genes (internal stop)      :: 37 of 200
            Pseudo Genes (multiple problems)  :: 43 of 200
            Pseudo Genes (short protein)      :: 5 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890340.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_9A"
                     /isolate="MINF_9A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="7"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            complement(join(4041..4062,1..680))
                     /locus_tag="JMW07_RS26555"
     CDS             complement(join(4041..4062,1..680))
                     /locus_tag="JMW07_RS26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1213..1467)
                     /gene="mobC"
                     /locus_tag="JMW07_RS26560"
     CDS             complement(1213..1467)
                     /gene="mobC"
                     /locus_tag="JMW07_RS26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1646..3793
                     /gene="mobQ"
                     /locus_tag="JMW07_RS26565"
     CDS             1646..3793
                     /gene="mobQ"
                     /locus_tag="JMW07_RS26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_070749924.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1469..1645)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1646..2004
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          complement(2..680)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.412"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1560..3790
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.059"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtcttcct tgaagcgaaa atgcagggct acaaccgtgc gccccttttt gatcgtctca
       61 accgttacaa ccagatcgct tttttcgttc agttcggcga tggctggctt gattatcatc
      121 ttattcaagt ctttgaattg ttgatattta tcttcaacgg caagcatggt tcggaaatca
      181 tcgatagcta tcgttctgtc accggtgctg cggaactgct ggagcagctc gtaaatgcgg
      241 atgctgtagg tgctggagag gctggagacg ttcttcacga ctacccgcgt aaactgcccc
      301 ttgagctgtg tcagatacgg catgatggca tcggagaagg tgatctctac tttcgcctca
      361 cctttgaaat actgcgcccg gtactgcacc cagcggaact cttctcgttt ttcctcgttc
      421 ttgatgatta ctgacctgtc ccagaggcga tctatcgctt ccttgagcct agcttcagcg
      481 tgctgccgtc ccatgtcagg aaaggcatca tgaaactctg cagccgtgat ggtcatggtt
      541 ttttgttcat ccggcgaagg ggaatcttct ccggatttca ggcggctgat acagaccagc
      601 aggaagcgct gttcctgcaa agtgagcttg tagcttgcct cgataagggc gtttgatttg
      661 taagctatca gatctgacat ttcgcttgct cgttgctatt ggtggcgttt ttttactgta
      721 cgccacccat catattggtg gaagtttttt ttttgcaacc ccttttttct gtggattaca
      781 cccccaaaag ctccacctat cacccccaaa agctccacct atcaccccca aaagctccac
      841 ctatcacccc caaaagctcc acccatctcg ccagaacacc tttaaaatca tgtggttaca
      901 agacctaaaa aggttttaaa aaaaaagatc taaaaataag atcttaaaaa cttaacggcg
      961 agcctgtgga taacttgggg tgctgaaaat aaaaatccaa taaaaacaat gtgttgaaaa
     1021 aaatcaggat tgaagattcc gaatacaccc cctccaaccc tgtggaaatc cagtattcat
     1081 gcgggttcag cgcatatcac ctgtcgaaaa accgtgcgta tgtgctcaac atggcgactg
     1141 attcacttcc gcttcctcgc ccactcgctc gctaacgctc ggtcgctggg ctgcggcgag
     1201 cggacgatac aatcactgct ctggcagcgc ctccagctcg ttaatcagcg gcagtattcg
     1261 cgtcacatca gcctgccgtg tcacctctgc cttcaggaac tggagtaacc aagctcgcaa
     1321 ctgcacttcc tccggctttc tgacttttgc cagcaatgct gcacccacaa tgaccttctg
     1381 cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt aatctcgcct cagcatcagc
     1441 aatcttctgt tcgagcgttc tgctcatgat gtaactccat gcgcgatgaa aaatcgcatt
     1501 ttagcgcgtc actggtagtt taaaaactga gctggcataa tgcacggcaa atcacgaagt
     1561 gcgcacttat acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg ctgaaagaaa
     1621 actgcaaaaa ggcattacgg cagaaatggc gatttatcat ctcagtatga aaatcatctc
     1681 cagaagcaac ggctacagcg ccgttgcttc tgctgcctat cgttctggtt cactaatgct
     1741 tgatgagcgt accggactga ctcacgacta cacccgcaaa agcggtgtgg ctgaggctgt
     1801 gattctgacc cccgcgacag cccctgcctg gtgtacgaac cgtgccgaac tctggaatgc
     1861 cgttgagaag gccgaacgcc ggaagaactc acaacttgcg cgggaaattg aactcgccat
     1921 cccccacgaa ctgccgcagg atgccgcacg ggaaaccgtt ctcgcctttg tcagagaaaa
     1981 ctttgtcagt cagggcatga ttgctgatgt ggcgttccat cacatggata aaacaaatcc
     2041 ccatgcgcac atcatgctga ccacgagggc tgtcgggcct gctgggttcg gcggaaaggt
     2101 cagggactgg aacgaccgga cacatgccga gacgtggcgt gcatcatggg ctgaccatgc
     2161 aaacagggca ctggcgaacg ccggttatca gaaagagata gaccatcgtt catacgagcg
     2221 acaggggctt gagaaaacgc cgggtattca cctcggaaag tctgcctgtg cgatggaaac
     2281 acgcggtatc gaaacagaac gcggcgaaca gaatcatctg attaacaggc ttaacctgga
     2341 aatacagatt tcccacaccg aactcaggaa cagcgggctg acagagcctg cccgccgtgt
     2401 ggctgacctt ctgaatatcg ggcttcctga caacgcaact tcgtacacac tacgtgacat
     2461 catcgaagcg ttgccgaagg acagtaacgc ggcatggaaa ctcaccagta acgccatgtc
     2521 gatgatggca gatatgcagg cgacgcgcag acgctgggaa gagctgaaca ccgaacgaaa
     2581 ggaagcgatg agccatgctg ccagcctgaa aaaatcatcg ccgttttcgt ccggtttttc
     2641 ccgtattccc ctgatgcaat gggctgcacc ggaataccgt agtgagcagg gaaaaatcca
     2701 gtctctgagc cagcaaatgg aaaaattgcg acagcattac cggaagctgg agaaacaaga
     2761 cattcccgcg agtcagacgg catttgaaaa acagtggcag cagtgggggg aatccggcct
     2821 tgcagctctc agatcccagt tgcttaaacg tgaagaggaa ttgcgccgga aggagctgga
     2881 ggatacagaa cgtagtcgcg cggagcagct caggaaaaat gacaatgccg ttatggattc
     2941 aggggtgctg agagcagtcg ttacggggta tggcgaggct cccatgcctg gtaacgggga
     3001 aatgacctgt taccttctgc ttcacaaccg gaacggagaa tatacgctct gggggaacga
     3061 gctggagaaa taccgcacca ggatctttga gagtgttgat ttaatgcgtg acaggagtgg
     3121 ttatatctgt gaccgaagcg aaatcggtca atatccacca ctgcaacgtg tttattcctc
     3181 tgcgaccttt gagcaacttc tgacgcaggt ttgccagaca tggccgcaac atacccgcaa
     3241 tctccgccaa ccgaagacat ggccggagtc tttctgtctt ggagaagacc gccagccagc
     3301 catgccatca ctggcagcaa ggaaggtgga ttttacgcag ggacgtctgc caccgacgct
     3361 gatgccggtc atgagttctg tcgaccggga aacccgccag cttcagcttt tgctggtcat
     3421 gggcgtggat gactctctgg gcggtgttgt ccggcttaat ggtacgctgt atcccgcgtt
     3481 tgccgttccc tcagccgata acagccagct ggtcatcagt gcactgaccg ataaggggtt
     3541 acggtatgca gggtatggtg ttgccgtaaa tcatgatgca gacagccaca tatcacctgc
     3601 gccggaactt atggagtttc atctgaaaac ccgtgaggca ccgctttttg ctgccgtcaa
     3661 cacaccagaa aaacagccgg atcatctgtt ccgctcgctg ggtttcaatc gtacctggga
     3721 cgaatggcgg cgcgaggaag atgcacgaac acacgccacc gagaggagac atgatcgcgg
     3781 ttggtcacag tgaatgtgaa caatattttg ttttgtttaa catcgtgtta tgttcgtcga
     3841 tgttcatcga cagggatgaa tgcaagaagc acactcacat aacgcaagca tagttttgtt
     3901 tttccgcccc caaaaattgg gggttctttt ttcgctgcat cagagcgttt ttgacgcaat
     3961 ttttcacttc aatagtacct tcgcactact aacacaaaaa aaacgcaaca gggagtgttt
     4021 taggtgcctt aaaatcgatt ctagatcgtc atcttgatct gt
//
