LOCUS       NZ_LR890339             4237 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_9A isolate MINF_9A plasmid 6, complete
            sequence.
ACCESSION   NZ_LR890339
VERSION     NZ_LR890339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357504
            Assembly: GCF_904863255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:13:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 5,054
            CDSs (with protein)               :: 5,054
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 73 of 200
            Pseudo Genes (incomplete)         :: 136 of 200
            Pseudo Genes (internal stop)      :: 37 of 200
            Pseudo Genes (multiple problems)  :: 43 of 200
            Pseudo Genes (short protein)      :: 5 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890339.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_9A"
                     /isolate="MINF_9A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="6"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            complement(join(4149..4237,1..625))
                     /locus_tag="JMW07_RS26530"
     CDS             complement(join(4149..4237,1..625))
                     /locus_tag="JMW07_RS26530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001590713.1"
                     /translation="MNKKLIFSVLLCLFSFGSVAGIPVAIDASPEWAVEATRWTERLKQ
                     WSETARHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELANIYKQGNSYISDFTSN
                     PEGALSSQAKNLFSKYGAFNMCSTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAMS
                     QIQSLSNRIESSKDMKESQDLANALQAQSLKIQAIKMQYDVWDSKNRADAEMLKEQKYA
                     EYRLRQKNAKIPSFE"
                     /name="type IV secretion system protein"
     gene            complement(622..1620)
                     /locus_tag="JMW07_RS26535"
     CDS             complement(622..1620)
                     /locus_tag="JMW07_RS26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000012467.1"
                     /translation="MSGFFEKFNSEVVSKIEEMTKPLQSQLGQDMLSLLSAGISVYILW
                     VGYQILAGKRQTPLQDLVWDLSKFAIILTFVSNADGYLTAAMDAIKSMKDGFLGKPVWA
                     TLDTMWSTTQLLADKVYAMDQSKYVSVEGGIGSMLVWGGSILIMSVAAVIYMLADVTMQ
                     LAILVAPIFIGCYMFGFTRVMFNNWLGILFSSVFTVIFAGVLVKIGTEFQTDMLSQISR
                     DANSTNILTMGAMAFVIGVLIAVFVWKSSSFAQQLAGAGVEGTLQGMALLGIGAAGMAV
                     EKTRNQMKRGTRTAAEWAGEKGEQKRASIGALGMEARRRASLEKARARNSA"
                     /name="type IV secretion system protein"
     gene            complement(1625..1888)
                     /locus_tag="JMW07_RS26540"
     CDS             complement(1625..1888)
                     /locus_tag="JMW07_RS26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000717655.1"
                     /translation="MKKICTLLMSIFILSACGEDTKSSDWWLNHPKEATEKYKECKKSG
                     EDSVNCQNVKKVAGIIGRTYGPMLEILKAESAEYDKQHGLNR"
                     /name="EexN family lipoprotein"
     gene            complement(1976..2266)
                     /locus_tag="JMW07_RS26545"
     CDS             complement(1976..2266)
                     /locus_tag="JMW07_RS26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_064765892.1"
                     /translation="MTKHDAIRISQLHQIFYDDDGLIDSEKNVTILYSIGFTVDEIAHF
                     RNSTPNTVAAQLLNARVKLGCASVSSLKPMILLRLLLNIKEIRFGFETDCK"
                     /name="transcriptional regulator"
     gene            complement(2447..2962)
                     /locus_tag="JMW07_RS26550"
     CDS             complement(2447..2962)
                     /locus_tag="JMW07_RS26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000097059.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNTG
                     EKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQVLL
                     DSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDRIAF
                     TTLIERDC"
                     /name="replication/maintenance protein RepL"
     Typing          complement(818..1620)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="89.552"
                     /coverage="91.0"
                     /evalue="1.15e-160"
ORIGIN
        1 ttttactatc ccatacatca tactgcattt taattgcctg tatttttaac gactgcgcct
       61 gcaatgcgtt agccaaatcc tgggattctt tcatatcctt tgaagactca attcggttac
      121 ttaaactctg aatctgagac attgcacttg atagctgctt atttatctca tttccctgtt
      181 caatactggc ggccgttgaa actattctgg ccttacaaag gttatcagaa ctttcaatgc
      241 ctgtagaaca catattaaaa gcaccatact tgctgaacag atttttcgcc tgactactta
      301 atgcaccttc aggattactg gtaaaatcag aaatataact gttcccctgc ttataaatgt
      361 ttgccagctc tgattgtagg ttacttagtt catttgtgaa tgccgcaata ttccttatac
      421 ctgttgccgt ggctaactgg tctttatatg cctgaatctg actttgataa tgccgcgctg
      481 tttcagacca ttgtttgagt cgttctgtcc atcttgtggc ttcaacagcc cattccggac
      541 tggcatcaat agcaaccgga atacctgcga cagaaccaaa tgaaaataag cacaaaagta
      601 ctgaaaaaat tagttttttg ttcatgctga attcctcgca cgtgcttttt ccagggatgc
      661 tcgtcttctt gcttccattc ccaatgcgcc tattgaagcc cgtttctgct cccctttttc
      721 acctgcccat tcggcggctg ttcgcgttcc acgtttcatc tggtttctcg ttttctctac
      781 ggccattcca gcagccccaa tccctaaaag agccattccc tgaagagtac cttcaacacc
      841 tgcaccggca agctgctgcg cgaaactcga tgatttccag acaaagacag caatcagaac
      901 tccaattaca aatgccattg cgcccattgt taaaatattt gttgagttag catctctcga
      961 tatctgacta agcatatcag tttgaaactc ggtaccaatt ttgacgagaa ctccagcaaa
     1021 aataacagta aatacactag aaaataatat accaagccag ttattaaaca ttactctagt
     1081 gaatccaaac atataacaac cgataaatat cggagctaca agaatagcta actgcattgt
     1141 tacatcggca agcatgtaaa taacagccgc tacagacata atcaggatac ttcctcccca
     1201 aaccaacatt gacccgattc caccttcaac tgacacatat ttggattgat ccatagcata
     1261 aactttgtca gccaaaagct gagttgtact ccacatggta tctagcgttg cccatacagg
     1321 tttaccaagg aacccatcct tcatagattt aatagcatcc attgctgccg tcagatagcc
     1381 atcagcattg cttacgaatg ttaaaataat ggcaaatttt gatagatccc agacgagatc
     1441 ttgtaatggg gtctggcgtt tccctgccaa tatttgatag ccaacccaga ggatataaac
     1501 gctaatacct gcacttagaa gagataacat atcctgacct aattgacttt gaagtggttt
     1561 ggtcatctct tcgattttac taacaacttc gctattaaac ttttcaaaga aaccagacat
     1621 gttatcacct gttcaatcca tgttgcttat catattcagc tgactcagcc ttaagtattt
     1681 caagcatagg gccataggtt ctaccaataa ttccggcaac ttttttaaca ttttgacaat
     1741 taacagaatc ttcccctgac tttttacact ccttatattt ctccgtcgct tctttgggat
     1801 gattaagcca ccaatctgat gattttgtat cttcaccaca tgcggaaaga atgaaaatag
     1861 acattaagag cgtacaaatt tttttcatct ttgattcctc gtatggacat tacaattgct
     1921 gatacaatca gcaattgtaa ttctagtcaa gagcaaaaat acctattgaa ctatattatt
     1981 tgcaatcagt ttcgaatcca aaacgaatct cttttatgtt caaaagcaat cttaataaga
     2041 tcattggctt taaagatgaa accgaggcgc aacctaattt aacccttgca tttaatagct
     2101 gagccgcaac tgtgtttggt gttgaatttc taaaatgagc tatttcatca actgtaaatc
     2161 ctatactgta taaaatagtc acgttctttt ctgaatcaat taatccatca tcatcgtaaa
     2221 aaatttgatg aagttgagaa atgcgtatag catcatgttt agtcataaaa acctctagcc
     2281 aggttgttgt gattagcagg cgaacaaaac accacttttg ttcgcctgcg actacttcta
     2341 gtagcctgcg gcaggcacag gcaaaaacat cgtttttgga caatcatgga taaaatgtta
     2401 acattttgac catgattgtc tatcctgcat tgtattgttc tatcgattaa caatccctct
     2461 caattagggt tgtaaatgct attcgatctc cattaaatac aaaattgggg tttataaaat
     2521 aaaatccttt acgcattgtc tttgctataa tctgagcttt ttctaactcg ttaattcctc
     2581 tctttaacgt agcataacta agttttaagc tatcatagct ttttaaaaag tcatcaagag
     2641 cccttgaatc caaaagaacc tgatcctttg caaaggcttg tttttgaaca gcccataaaa
     2701 gaacattaaa agctttaatt cctgccgatg ataaatcaaa cgttaaccca atattcgcag
     2761 taaacaactt aacaaattgc tcactatcta cacgcttaaa agtggttaca tgtgttccaa
     2821 acttttctcc cgtgttttga ttaaccaaaa cattgtcatc ccgcccaaga cgagatactt
     2881 gtatccgttt ccctttcatc gggatcgtca taagctctaa aaacgggttt gtttcgtatc
     2941 tctgtaagct tagcgagctc attattgcat ccttttttgg gctttggaga ttcacaataa
     3001 atcctcataa gctcaaaatc aagcaatttt tgagcctgca aggtaccgga tctgagctta
     3061 caaggtacag atcctgatca ccactttcta ggattcatgc gggttccgag aatttccctt
     3121 cttagttctt aactaaggag cgtaggtgct caattatggc tatcaattct cttccgcttc
     3181 ctcgcccact cgctcactac gttcggtcgc tggggctgcg gcgagcggga tcacctctcg
     3241 catgttgtca gaaatgccgt tgctgttctg ttcgaaaatg cgttctgtgg gccatccaga
     3301 gcaccagaac gcgattttca ggtgttggta atgttttagt cgtcttagct cttaaaacgc
     3361 gctcagacgc acgaaacagg ctttagatgc ccgtcagggc atggaaggcg cgaaaaatgc
     3421 ttacagaggg tttcaggagg tcaggcgcgc cgatacccct aaccaggggc tttcaggtcg
     3481 ccaggaaccg cgccagcccc ctgcaaagcc cattagcacg gaacaggtag ccacacggtt
     3541 ggtaggtggt cgtagaccgt cgatgttaca gcgatggggg tctggggttc tccccagacg
     3601 gtttccggtt gcgctcaagg cgctgccgga accgtggcgg ctcggtggtt ctagagatcg
     3661 ggaaacaaaa acacgctaga ggttttgagg tcggcaaaag agcaatgcag gatagacaat
     3721 ccgatgtcaa atgttaacat ttgaatcaga ttgtccaaaa acgatgtttt tgcctgtgcc
     3781 tgccgcaggc tctatattta gaatttagaa tagaaaaggc catctattcg atgaccttta
     3841 ttttttactt cattgaaaat cgcattacta ttaataacag aagtaaaata caaaacagct
     3901 ttaacgtaaa aaggagtctt tcttttatac caccgctggg aacaaacctc ttttcaattt
     3961 tatcagtgat aaattgcaat gtaccataat acattaaagc attaaccagc tttaacgtaa
     4021 gctccacact taaaacaata actatgattg gtagcgagat taacagtggc ggcggtgtag
     4081 aaccggtact attcaatcta gaccaatgat tatctaccca ttcttttagc ttatttagct
     4141 ttatcatatc attcgaaact cggtatcttt gcatttttct gacgcaggcg atactcagca
     4201 tacttttgct ctttcaacat ttccgcatca gccctgt
//
