LOCUS       NZ_LR890332             3608 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_1A isolate MINF_1A plasmid 7, complete
            sequence.
ACCESSION   NZ_LR890332
VERSION     NZ_LR890332.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357499
            Assembly: GCF_904863375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:46:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,250
            Genes (coding)                    :: 5,034
            CDSs (with protein)               :: 5,034
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 72 of 216
            Pseudo Genes (incomplete)         :: 147 of 216
            Pseudo Genes (internal stop)      :: 32 of 216
            Pseudo Genes (multiple problems)  :: 33 of 216
            Pseudo Genes (short protein)      :: 4 of 216
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_1A"
                     /isolate="MINF_1A"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="7"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            join(2632..3608,1..541)
                     /locus_tag="JMV91_RS26560"
     CDS             join(2632..3608,1..541)
                     /locus_tag="JMV91_RS26560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_020833675.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRST
                     DIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGIGDCVLINNNIANDISIFNM
                     ANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYVSPLA
                     EYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLFARRFE
                     KNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESI
                     SGENGNLRGYIFELFVSSQICNFYGYGNVRINKEYKINGKHAEADVVLETNDDIYIVEC
                     KNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDEFKC
                     NNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     gene            complement(746..904)
                     /locus_tag="JMV91_RS26990"
     CDS             complement(746..904)
                     /locus_tag="JMV91_RS26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532985.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVLQSS"
                     /name="replication initiation protein"
     gene            complement(1400..1594)
                     /locus_tag="JMV91_RS26565"
     CDS             complement(1400..1594)
                     /locus_tag="JMV91_RS26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039023183.1"
                     /translation="MNKQQQTTLNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFESESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1879..2202
                     /gene="mobA"
                     /locus_tag="JMV91_RS26570"
     CDS             1879..2202
                     /gene="mobA"
                     /locus_tag="JMV91_RS26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          1633..1870
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="99.0"
                     /evalue="2.15e-118"
     Typing          complement(837..1357)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.905"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          1880..2199
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.14e-62"
ORIGIN
        1 tcctgatgag ttacttcaaa tggtaaaaga tctggagtct atctctggag agaatggaaa
       61 tttaaggggg tatatttttg agctttttgt ttcctctcaa atttgtaatt tctatggtta
      121 tggaaatgtt cgtattaata aagaatataa aataaacggt aaacatgccg aagctgatgt
      181 tgtattggaa actaacgatg atatttatat cgttgagtgc aaaaatgtta agactttacc
      241 ttcgatggag gttagtcttt ggatgaaaac aagagtgcca attataaatt cctactacaa
      301 atcaaataac cctgataaca aaaatataca tcatcgtttg tgggttacgg ggaatatata
      361 tcctaaagat ataaaccgat tggacgagtt taaatgtaat aataagaaga ttgatgttga
      421 ttatttattt ggtcagtctt tggatgattt tttctaccaa aaaaaacaag tgtttaattt
      481 atataggaaa gtcatttcgc cagattataa aaaagggcaa atacccgatt ttgacttata
      541 gaatgacgtt ttctagtctc tgcatttctt gacgatgacg gcttgaaaag cgtatcttcc
      601 tccatgaaac gaaaaaacca cccggcaagg tggtttttcg aaggttagtt aatcctggca
      661 gattacctaa cctaggtaac tggcttcagc agagcgcaga taccaaatcc tgtcctttaa
      721 gtgtagccgt agttggggca tcacttcaag aactctgtag tacggaccgc attatcatcg
      781 ctctgctatt ccagttacca gtggctgctg ccagtggcga taagtcgtgt cttttcgggt
      841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      901 gcacacagcc cagcttggag cgaacgacct acaccgggcc gagataccaa cagcgtgagc
      961 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaaacggca
     1021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1081 gtcctgtcgg gtttcgccac ctctggcttg agcgtcgatt tttgtgatgc tcgtcagggg
     1141 ggcggagcct atggaaaaac gccaccggcg cggccttttt cgggttcttg ttttttgctc
     1201 acatgtttct tttggcttta tcccctgatt ctgtggataa ccgcattacc gtctttgagt
     1261 gagctgatac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag
     1321 cggaagagcg ccttatgtgg cattttctcc ttacgcaccg gtataccgtt ctgcatcctg
     1381 tctggagcag gatacactgt tataccccgg tgccactttc cgattcaaag cgtgtctgta
     1441 tgctgttctg gagttcttct gcgagttcgt gcagtttctc acacatggcg gcctgtgcat
     1501 cagcatcgag tgagtccagt ttttcgagca gcgtcaggct ctggcttttt atgaattttg
     1561 ccatgttaag tgtggtttgc tgctgcttgt tcatctttct gttttctccg ttctgtctgt
     1621 catctgcgtc gtgtgattat atcgcgtacc acttttcgac agttttgccg ccggtattct
     1681 gccgtctgac tctttgacgg gcatttctgc ctgacaatac tgtcactgcc aaaaaactgc
     1741 cgtgcctttg tcggtaattc gaacttgctg acaggacagg atgtgtaatt gttataccgc
     1801 gcatacatgc gcgctattac aattaccctg gtcagggctt cgccccgaca ccccatgtca
     1861 gatacggagc catgttttat gacaaaacga agtggaagta atacgcgcag gcgggctatc
     1921 agtcgccctg ttcgtctgac ggcagaagaa gaccaggaaa tcagaaaaag ggctgctgaa
     1981 tgcggcaaga ccgtttccgg ttttttacgg gcggcagctc tcggtaagaa agttaactca
     2041 ctgactgatg atcgggtact gaaagaagtt atgagactgg gggcgttaca gaaaaaactc
     2101 tttatcgacg gcaagcgtgt cggggacaga gagtatgcgg aggtgctgat cgctattacg
     2161 gagtatcacc gggccctgtt atccaggctt atggcagatt agcttcccgg agagaaactg
     2221 tcgaaaacag acggtatgaa cgccgtaagg ccccaaaccg atcgccattc actttcatgc
     2281 atagctatgc agtgagctga aaacggtcct gacgcatttt tccggttttc actgggtaaa
     2341 acatctcttt ttgcggtgtt tgcggcagaa tcgcgttcag cgcgttttag cggtgcgcgt
     2401 aattaaggga ttatggtaaa tagggcggtt gatatatgat gatgaaatgt cacaaacgta
     2461 acatcttgat ttttaaggat aaatctcgca ggtgtgtttt ttgtgatatg ttgcttttgt
     2521 taaatgtttg atttttatga tttttttctt tgtgtttttt ttgggatcta tagatattac
     2581 acgtagtgta attttataaa tagcgtagtg tgacaaaaat aagatgaatt tatgaaaaaa
     2641 aacattgtta gacaagtgct tgagagttat ggtccgtgca tatcttctga acttgctgag
     2701 aggattaagc gtcagaatcc ttctatgtca tctgatgcaa tacgtaaaat gatctctcga
     2761 agcactgata ttggtaagtt gccattttta agatttagtc ataacaggag attcatttat
     2821 ctaaaagatg actttggtag cttcaaattt tggcgggcgc tcaaaaaatg tatgcgtgag
     2881 gcaaattcgg cgtattctca cgctatactt tccgtaatta ataatggcgg atacttgaag
     2941 gtaaaggatt ttgggattgt gtctggctct ccgataaagc aggccaagca tttaagttat
     3001 gaatctgttc taaaaaatct gctttcagct aaaatactta gatctgtgta tattgatggt
     3061 attggtgatt gtgttttgat aaataataat atagctaatg atatcagcat ctttaatatg
     3121 gcaaattgcg agtctttttt tgataagcca atatttgaac tgattaaggc atggttgcga
     3181 aatttaggta ttgtggcttt taatcaaata aaaacaaaat atgatggaga gaacaatcct
     3241 gtggttggtt ctttcgagtg ggatataaca gcgccaagtt atgtgtcccc tcttgctgaa
     3301 tattccagcg atggactcat cccaggtttt gttgtctgtg attttgctct agggtttaat
     3361 cgaaatgaaa ttaccaccga tgctgctgat accttcataa gaaaagttca gatgaccaag
     3421 tcctctagga caaaccagag gattatgttt gttctttttg ctagacgttt tgagaagaat
     3481 gccttcaaca agctaaggag tgaaggggtt ctggctataa ctatagctaa tgcgtttggt
     3541 aataaggtcg atgagagctt ggccagacta tcgaaggtga ttcagggaac tttatctatt
     3601 gaaagaca
//
