LOCUS       NZ_LR890298             5393 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_1B plasmid 8, complete sequence.
ACCESSION   NZ_LR890298
VERSION     NZ_LR890298.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07738870
            Assembly: GCF_904863435.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:09:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,559
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,298
            CDSs (with protein)               :: 5,298
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 58 of 133
            Pseudo Genes (incomplete)         :: 80 of 133
            Pseudo Genes (internal stop)      :: 23 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890298.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_1B"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="8"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2013"
                     /collected_by="Adam Jenney"
     gene            complement(join(4938..5393,1..108))
                     /locus_tag="JMW65_RS27690"
     CDS             complement(join(4938..5393,1..108))
                     /locus_tag="JMW65_RS27690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087842647.1"
                     /translation="MNDMKAVRHRYKQRYDFAASEVVGAFDELRSLDWSLEIASLSDVE
                     MSKRWIGAVDPNGVARLPPNGGWDWVKLYKGYLKNPSAFVCAIKSEGKLCALIGGGVSD
                     SKVVTKLHYLESAPYDTALSGYALDISVTYVVTVANAMGSEYSAIYEPNSKVREIAIND
                     FGFSEDNLFGTSKDFALYLPMSRI"
                     /name="hypothetical protein"
     gene            complement(105..623)
                     /locus_tag="JMW65_RS27695"
     CDS             complement(105..623)
                     /locus_tag="JMW65_RS27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001445155.1"
                     /translation="MSEKAKKVGRRSLSKRRESLIKELWGDELSELNIWNRKEHDGFTT
                     IPRTLNYINRIIDYLAGAGSPVSQTYLSLWCRVFDEGFVEIRDKDALAFESGFSGQRAV
                     TTWLGRMKKLRELGLISTKPGTSGEFHYVIMLNPLYVIKKSYESNGWARDERYNALFSR
                     MQEVGAEWE"
                     /name="hypothetical protein"
     gene            1525..1854
                     /locus_tag="JMW65_RS27700"
     CDS             1525..1854
                     /locus_tag="JMW65_RS27700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863266.1"
                     /translation="MDYQKVEIEMTGKQRLEFNGKEVSRTKDNKYIVYETPKGHWVIVE
                     REFRSKPSAHRGGKPEIIPTGRDEDYKIIKDKNQSALFEHIGFNKHLDEICAGLGINPA
                     KSLDV"
                     /name="hypothetical protein"
     gene            complement(1977..2192)
                     /locus_tag="JMW65_RS27705"
     CDS             complement(1977..2192)
                     /locus_tag="JMW65_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_050863267.1"
                     /translation="MTELEKQLLNALEQLQQDYSRRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLHRLSNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2199..2696)
                     /locus_tag="JMW65_RS27710"
     CDS             complement(2199..2696)
                     /locus_tag="JMW65_RS27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140411320.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDLKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYMSIREQKDALQKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGRKNGIRLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2731..3435)
                     /locus_tag="JMW65_RS28070"
                     /pseudo=""
     CDS             complement(<2731..3435)
                     /locus_tag="JMW65_RS28070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3374..3697)
                     /locus_tag="JMW65_RS27720"
     CDS             complement(3374..3697)
                     /locus_tag="JMW65_RS27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176372927.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQINAGGGSGHDRVQVVAALMAIDAGLERLRYAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4334..4480
                     /locus_tag="JMW65_RS27980"
     CDS             4334..4480
                     /locus_tag="JMW65_RS27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863271.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHAKAPLAAANGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     Typing          complement(3799..3984)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="4.5e-50"
     Typing          3964..4443
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.535"
                     /coverage="93.0"
                     /evalue="3.49e-179"
     Typing          complement(1892..3384)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.178"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 taaactccaa tccaaacttc taagttcatc aaacgccccc actacttctg acgcagcaaa
       61 atcatatctc tgcttatatc ggtgacgaac agctttcatg tcgttcattc ccactccgcc
      121 cccacttcct gcatcctaga aaatagagcg ttgtaacgct catcccttgc ccatccattt
      181 gattcatatg actttttaat tacgtaaagt ggattcaaca tgattacata atgaaactcg
      241 ccagaagtac caggcttcgt tgatatcagc cccagctccc gtagcttttt cattcttcct
      301 agccaagtag tcaccgctcg ctgcccacta aaccctgact caaatgccag agcatcttta
      361 tccctaattt cgacgaatcc ctcgtcaaaa actcgacacc ataaactgag gtatgtctgt
      421 gacacaggag atccagcgcc agcaaggtaa tcgattatcc tattgatata attcaatgtt
      481 cttgggattg ttgtaaatcc atcgtgttct ttcctgttcc atatattcag ttcggaaagc
      541 tcatcccccc aaagctcttt tattagactt tctcttcttt ttgatagaga gcgcctacct
      601 actttttttg ccttctctga catttatccc ccaaaagtca attcaacatg aaaatattat
      661 caacaaaaaa cccattatat tcaatgagtt atgtgtaata acacaaaaac caagcacatc
      721 attaaccaac atctcatttg atatgattct ttttttttgt caatacaaat atctagatgt
      781 aatatttttt acaattatgg ttttattttt caattagtta tgcgccattt tgtactcgct
      841 gttctcaatg tgctctaatg taatcgttgt gctcgctgtg ctgaatgggg ttcttagggg
      901 gtttaagggg gggggaaggg gtgccctgaa tgcgcaatga aaaagcccac ataagcgggc
      961 ttcgtctttt taaatgattc ctcgacgtcg tagttcggct tcaatagcat cacaggaatc
     1021 acgcaaaatc ttcgtgttgt agtacctacc catctttctt cggataatat cgtcaagcaa
     1081 ggcattgtta tggtctaacg ttttgagtag gtatagggtt tctctatcca tcttaggatc
     1141 tccatttttt gttgatgagc ttattgctcg ccagtattta ttccgtctgc aaattaccta
     1201 ctcagaatgg cgtcaaaact cgagttttcg cttacacctc agaaaccatt taccataaca
     1261 tcccttgccc gcaccgccca ggatgccctc agcgcgttta tgcggcctcc gggccgtttt
     1321 cggtaccttt ctcagtccat ataacggaaa aatgcccttg gtgatttctg tgcacctgaa
     1381 tggctatgca cgtaaatgaa tggctgactg ctttcatgct acagatgaaa aattgatgtt
     1441 cttttttacg ctgttctggt ttagagttct cattgcaata aaagcaaaaa tcattcccaa
     1501 acaataactt aaaaaggttt ttatatggac taccaaaaag tggaaattga aatgacaggt
     1561 aaacaaaggt tggaatttaa tgggaaagaa gtctctcgaa caaaggataa taaatacata
     1621 gtttacgaga ccccaaaagg gcattgggtt attgtcgaaa gagaattcag gagcaagccg
     1681 tctgcacata gaggcgggaa gccagaaata attccaacag gacgcgacga agactacaaa
     1741 atcattaaag ataaaaacca aagtgcgctt tttgaacaca ttggatttaa caagcacttg
     1801 gatgagatct gcgctggatt aggcattaac ccagcaaaat ctttagacgt atagatcaag
     1861 cgactaaccc agtgacggac tgtcgagaga tttcgatttc aatgagtttt ttaacgccag
     1921 ctctttctga tgctggcgct cgacctcttg gttatgcaga cgcagctcga cagcgttcag
     1981 ctgtttgaca agcggtgcag ctgctcgctc aaactctgca cctgcctgct caagcccgtg
     2041 acacgctcgc tcagcgccgc gttctcccgt tgcataagac cagacatcgt ccgccattcc
     2101 gcgaaggcgt tctcccactc gtccagcctt ctcgagtagt cctgctgtag ctgttctaat
     2161 gcgttcaaca actgcttttc cagctccgtc atgtcgctct actcctgcac cagtctgatg
     2221 ccgttcttcc tgccgttgtc gaacgtccag ttcgtgtccg ctttcacgcc ttccggcagc
     2281 accagaaagc gcccctggtt gtcctcttgg tatctcacgc cccacgtccg cgcgttcagc
     2341 ttctgcagtg cgtccttctg ttcccggata ctcatgtaat tgtcgagtat ctgctgcccc
     2401 tgccaccaca gaatgcccgc gctcgaggcg atcagcagaa cggacaccag gacgatggtc
     2461 agccacgtct ggctgaccat gcgcagcatc cctttcgtgc gctggctcat ggctgaggat
     2521 agcttcaggt cgtggtcgag gatggcggcg ctgattctct tctcgctttc gctcagttcc
     2581 gctctgacag acttctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtagtctgc
     2641 tgctgcgctt tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact gttcatagat
     2701 ggctcccttc agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt
     2761 tgtggtgcga actacctcaa aacctgcgcg ttccagcgtc tcagtgacgt cctgacgcgt
     2821 tttcagctct ccggacgagg ctaaggccag taaacctcgt gtaatcgcct gagcagcttc
     2881 ctgcttcttt tccggcagtg cggacggcgt gaccagtgcc cgccggttct ccggtgcgtt
     2941 cgggtcgtgc agccccagcc tgccgttcac gatggtctgc caggcatcaa tgcgcggacg
     3001 gtcggcgcgg tcgtaatacg gctggagacg cctgccggtc agcagctccg tgttcgggat
     3061 caggaagttc agctccagcc gccccttgtc ctggtgctca acccacagca cgctgtactg
     3121 gtctttatcg agtccgggca tcagaacccg ctcgaagctc gccatcagct tttcacgctg
     3181 gccgggcggt aagtcctgtt cggcaaagga caggacgccg gaggtgtact ttttggcgta
     3241 gggtgaggcg tcgataagct cccggacttc ctccggcttc ccctgaagaa cgctggcacc
     3301 ttcgcgctgg cggtctttcc ccagcagata atccaccggc ccagcgccac cgccgcgccc
     3361 tcgagggtga aacttaacga tcatcgtcag cccccttttc cagtacggca taccgcagcc
     3421 gctcgagtcc ggcatcgatg gccatcagcg cggcgacgac ctgcacgcgg tcgtgtccgc
     3481 tgccgccacc ggcgttaatc tgccgggcaa tctggttaag gttattcccc ataccggcaa
     3541 gctgacgaag cagcgccggc gagatcgagg gaagtttgcc ggcgcgggca ggcttctcgt
     3601 ccaagcacgt ctggcgcatc caggctgcga gctgtttacc gtcgcagcgt tccagcagac
     3661 ggcggtgctc atcttcggtc acccacatcg tgagcatttt gctgcgtttg tcgaccaatc
     3721 cggcctcctg ataacgccgg gacggtcggg aaaagatttc ccggtcggct cggtgttgga
     3781 caagccgcag gcgcgtcagt ggttttagca ggtttcgggg cgcagccctg aaccagtcac
     3841 gtagcgctag cggagtgtat actggcttaa tatgataaag tggagtggtg gtacagggaa
     3901 gtgcttcatg tggcagggtg aaatgcagat gcagagcgta agtgcataat gcaggtaatg
     3961 atgaaatgac gcctcctcgc tcactcggtc gctacgctcc tgccgtgaga ctgcggcggg
     4021 cgttgccaga tcacagatga tggcgagcaa acaagacagg gaccagaaag ggcaaaaaaa
     4081 agaggaggcg gtgccgtttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     4141 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatcccaggc gtttccccct
     4201 ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     4261 ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     4321 caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa
     4381 ctatcatctt gagtccaacc cggaaagaca tgcaaaagcg ccactggcag cagccaacgg
     4441 taagaggatg tacaagagat tactaacgca gagttcttga agtatcagcc caactacggc
     4501 taaactggaa ggacgaattt ggtggcagtg gtcttgtaca ccctgttacc acggttcata
     4561 aacttgccgg tttaatgaac cttcgaaaaa ccaccttgcc gggtggtttt ttcgttttca
     4621 aagaagatac gcgtttactc ttcgcctcgt caatgaaggg tcgcattaat tctgcgactt
     4681 gcgcatctct tctacaatac agtacagtta ctatgaagtt gacgtatccg tcacctcata
     4741 tgctttgtac taactcacga aaaaacggaa ataatatgtt agctcacgcg gtcgaactta
     4801 aaaaagcgac ggaaccatgc tcttctggca cagccggagc atttcgccag cgcgtgaaag
     4861 caagacgagc agagcaaggc cgatcaaacc aacagccaga aaaactctac ggcgtactgg
     4921 taggataaat gagccggtta aatccggctc attggtagat acagagcgaa atctttactt
     4981 gtcccaaaca agttgtcctc gctaaatcca aaatcattta tagctatctc tctgactttg
     5041 ctatttggct catagatggc tgagtattcg cttcccatcg cattcgcgac agtaacaaca
     5101 tacgtaacag aaatatccag tgcatatcct gaaagagccg tgtcataagg ggctgattca
     5161 aggtaatgca gcttagttac cactttacta tcagaaaccc cgccaccaat cagagcgcat
     5221 agcttgcctt ctgatttaat ggcacagacg aatgcgctag ggttcttcaa atagccttta
     5281 tagagcttaa cccagtccca tccaccgtta ggtggtagtc ttgcaacacc gttaggatca
     5341 acagccccaa tccatctctt tgacatttcc acgtcactta aagatgcaat ttc
//
