LOCUS       NZ_LR890296             6134 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_1B plasmid 6, complete sequence.
ACCESSION   NZ_LR890296
VERSION     NZ_LR890296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07738870
            Assembly: GCF_904863435.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:09:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,559
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,298
            CDSs (with protein)               :: 5,298
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 58 of 133
            Pseudo Genes (incomplete)         :: 80 of 133
            Pseudo Genes (internal stop)      :: 23 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_1B"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="6"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2013"
                     /collected_by="Adam Jenney"
     gene            join(5728..6134,1..505)
                     /locus_tag="JMW65_RS27610"
     CDS             join(5728..6134,1..505)
                     /locus_tag="JMW65_RS27610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_201525432.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSVRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDKWLQACYERASAYNLQLAAPLD
                     ENEISGIAKSIAKWTYRNFTESSFEEYVKNTHTSKIQSSRGKNSRGGGRKVNIESERQT
                     MPWLKYNVSRSTYYRMKKNGKD"
                     /name="replication initiation protein"
     gene            492..1865
                     /locus_tag="JMW65_RS27615"
     CDS             492..1865
                     /locus_tag="JMW65_RS27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577352.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_201525433.1"
                     /translation="MEKIRRFEIDGLFNRFNYSIDMSIGELPGLSIISAPNGYGKTTIL
                     KMIASFSTGDFLIFFKEKYKAVRFFLTSDEYIEITRGVDESDDTYISLSNGVETSKITN
                     PLSDNGFVNIVNVIDKYLPYLRRVGPRKWRDDRVGDIISLQMALSRYKSHPALKRTMRK
                     RDVWVDKIISTLNVFTISTNRLSSDERLILRDDSEKNLMVVNIAENIKDKINEAIRNQF
                     EDGRKKETSFPTRIIEALSNQSFPSKESVLVDINKLKKLEDKYSRLGLIPNTETTNQFN
                     THIKGNSANGAGLLVLKTYLEDVLEKFALLDDLAEKLDIFTSSINSLLSFKEVMTTIDD
                     GFVVKLIDGDETPLDLESLSSGEQHLLVLIGRLIFETSKECMVLIDEPEISFHPEWQER
                     FIGILSEIRKMNGFDVVLATHSPVLIGDLYWDYVVELSDQYSPSEKVLVSDIDISLGE"
                     /name="AAA family ATPase"
     gene            1867..2745
                     /locus_tag="JMW65_RS27620"
     CDS             1867..2745
                     /locus_tag="JMW65_RS27620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_201525434.1"
                     /translation="MSKKLRDYSVSSAVSTSLTMISGKNKRRTVVVEGVNDVRFFNQWY
                     GDSNKVRFVSVGGKDKVKSTYDSFLKNSRAKSLRCMFFCIDIDWDFLHEKKMPDTNFFM
                     ANSFCLNNNVFYHNDMECFLLNTVAIKKVLASYDYNMEDNALEDLLVKMESASRAIGKY
                     RAADEVVRRKFGLYSSVLNGLNELSYFDCSNFSIDEVRLQSDMPQWSNYAEHVDDLIEE
                     ANALDQANPTPWALTHGHDVTSMLSEYLKCTHGVTGLHRDKLEKELRLSCELADFHSTP
                     MYKKLSLESLC"
                     /name="hypothetical protein"
     gene            2979..3305
                     /locus_tag="JMW65_RS27625"
     CDS             2979..3305
                     /locus_tag="JMW65_RS27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193431.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQTDSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3514..>3936
                     /locus_tag="JMW65_RS27630"
                     /pseudo=""
     CDS             3514..>3936
                     /locus_tag="JMW65_RS27630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3983..4468
                     /locus_tag="JMW65_RS27635"
     CDS             3983..4468
                     /locus_tag="JMW65_RS27635"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029504503.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSAAMAWVFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLANLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            4538..4762
                     /locus_tag="JMW65_RS27640"
     CDS             4538..4762
                     /locus_tag="JMW65_RS27640"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029498753.1"
                     /translation="MEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALTHSD
                     RVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4918..5250
                     /locus_tag="JMW65_RS27645"
     CDS             4918..5250
                     /locus_tag="JMW65_RS27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000017531.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSVRVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5252..5536
                     /gene="nadS"
                     /locus_tag="JMW65_RS27650"
     CDS             5252..5536
                     /gene="nadS"
                     /locus_tag="JMW65_RS27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     Typing          3296..4854
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.379"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggacactgg aaaattgccg tatggcagcc ggaactctac acgctggact ggctggctga
       61 ctttctcgac ctgaacgcgg cgaacgacaa agaaatcgtt gctgactacg gcttaggccg
      121 taactgcacc ctgttcgaca aaacccgcaa gtgggcatac cgtgccatcc gtcagggatg
      181 gcctgagtac gacaagtggc tgcaagcctg ctacgaacgc gccagcgcct ataatctgca
      241 actggcagca cctctggatg aaaatgagat tagcggcata gctaaaagta ttgccaaatg
      301 gacgtataga aattttactg aatcctcttt cgaggagtat gtaaaaaata ctcatacaag
      361 taagattcaa tcatcgaggg gcaagaacag ccgtggtggt ggcaggaaag ttaacattga
      421 atcggaacgc caaactatgc cttggttgaa atataatgtc agtagatcaa catattacag
      481 gatgaagaag aatggaaaag attagacgat ttgaaattga tggtttgttt aataggttta
      541 attactcgat agatatgtct atcggtgagt tgccgggatt atccataata tcggcaccaa
      601 atgggtatgg aaaaacaaca atattaaaaa tgattgcatc attttcaact ggtgactttt
      661 tgatcttttt caaagagaaa tacaaagcag taaggttttt tttaaccagt gatgaatata
      721 ttgagataac cagaggagtt gatgaatctg atgatacata tatttcccta tcaaatggag
      781 ttgaaacatc aaaaataaca aatccgctaa gtgataacgg atttgtgaat attgttaatg
      841 tgattgacaa atacttaccc tatcttagga gggttggacc tagaaaatgg agggatgata
      901 gggttggtga tatcatttca ttgcaaatgg cattatccag atataagtca caccctgcac
      961 tcaagagaac aatgagaaag agagatgtat gggtcgataa gataataagc actttaaatg
     1021 ttttcaccat atcaacaaac aggcttagct ctgatgagcg tttgatttta agagatgatt
     1081 cagaaaaaaa cttaatggtt gttaatatag ctgagaatat taaagataaa ataaatgagg
     1141 caatcaggaa tcagttcgaa gatggccgaa aaaaagagac tagcttccct acaagaataa
     1201 tagaggcttt gtctaatcaa tcgttcccga gcaaggagtc tgttctagtt gatatcaata
     1261 agcttaagaa attagaggac aaatacagcc gtctaggttt gatacccaat actgaaacaa
     1321 caaatcagtt caacacccat atcaaaggta actctgccaa tggagcaggt ttactcgtcc
     1381 tcaaaactta cctagaagat gtattggaga aatttgcact tcttgatgat ctggctgaaa
     1441 agttggatat atttacttca tcaataaata gtttgttatc cttcaaggaa gtaatgacaa
     1501 ctattgatga tggatttgtt gttaagttaa ttgatggtga tgagacacct cttgatctag
     1561 aatccttatc ctctggcgag caacatcttt tagtccttat aggtagattg atatttgaaa
     1621 ccagcaaaga atgcatggtg ttaattgatg agccggaaat ttccttccat ccagaatggc
     1681 aggaaagatt cataggtatt ctttctgaaa taagaaagat gaatgggttc gatgttgttc
     1741 ttgcaacgca ttcacctgtg ctaattggtg atctttattg ggattatgtt gtagagttga
     1801 gtgaccagta ttcacctagt gagaaagtcc tggtttccga tattgatatt tcattgggtg
     1861 aataacgtgt cgaaaaaatt gcgtgactat tctgtttctt ctgcggtatc aacctcatta
     1921 acaatgatta gtgggaaaaa taaaagaaga acagtagttg tagaaggagt taatgatgtc
     1981 cgttttttca atcagtggta tggagatagt aataaggtaa gatttgtttc tgttggggga
     2041 aaagataaag taaaatccac ctatgattcg tttttaaaaa attctagagc caagtctcta
     2101 agatgcatgt ttttttgcat tgatattgat tgggattttt tacacgaaaa aaaaatgccc
     2161 gataccaact tctttatggc aaattcattc tgtcttaaca ataacgtttt ttaccataat
     2221 gatatggaat gctttctcct taatacggtt gcaataaaaa aagttttagc ctcttatgat
     2281 tataatatgg aggataatgc tcttgaagat ttactcgtta aaatggaatc tgcctcacga
     2341 gcaataggta agtatcgtgc tgcagatgaa gtggttagaa gaaagtttgg actatacagc
     2401 agtgtattga acggtttaaa cgaactctct tattttgatt gctctaattt ttcgatcgat
     2461 gaagttagac ttcagtccga tatgcctcaa tggtctaact acgctgagca tgttgacgac
     2521 ttaattgagg aagcgaatgc tcttgatcaa gccaatccta caccatgggc tttgacgcat
     2581 gggcatgatg tcaccagcat gttatctgag tatttgaaat gcactcatgg cgtgactggc
     2641 ttacacagag ataaattaga gaaagaactt cgcctgagct gtgagctagc tgattttcat
     2701 tcaacaccaa tgtataaaaa actatcactc gaatctctct gctgaggcgt gagaccaaaa
     2761 caacctatat cagataacag ccctctttta agagggcttt ttttttgcag ggagtacaga
     2821 acctgacata ctacacttct cttatagcca gtacacacag cgtcccctct aacgaggtgc
     2881 ctccgtgact ggttcagggg gcttgccgcc ccccgaaacc cccagcatcc actgcgaaaa
     2941 attctcacag tggtgcgaat tttttctcag cggagcctat ggaaaagcga acgaaagaga
     3001 tcaaaatccg gctcaccgaa accgagcatc agcgactgct tgatcgctgc gaccggactc
     3061 accttgcgga gtggctgcgt cagctaggtc tgggtgaaca cacctcacgc aagcgccgtg
     3121 tgcctgaggt ggcgcccgaa ctgcttcgac aggtcagcgg gattggcaac aatctgaacc
     3181 agattgcccg ccgcctgaac cagactgatt ccctgacgcc ttctgaacgt gcctcgttgc
     3241 tcgtcgtact gaccagcctt gaccgtcagc ttggcgacct gctggagcag aaccgtgatc
     3301 gttaaaattc actcacgcgg tgcaggttcc ggcagtggtc ctgtggatta cctgcttgga
     3361 aaagaccggc agcgcgaaca ggccagcgta ctgcgcggca atcctgaata cgtccgcgag
     3421 ttgatagacg gctgcgactt tgcccgcgcc tatacttccg gcgtgctgtc atttcaggag
     3481 ccggacattg cagatgccga gaaatcacgc ctgatggacg aatgggagta cacgctgctg
     3541 cctggtctcg accgtgacca gtacgcctgc ctgtgggtgg agcaccgcga caagggacgg
     3601 cttgagctga attttgtcat cccgaacatc gaattgcaga gtggcaaacg cctgcaaccc
     3661 tactttgacc gcgctgaccg cccccgcgtc aacgcctggc agaccctgac caacgaccgg
     3721 ctggggctgc gtgacccgaa cgacccgacc taccgccgcc cgctgacaca ggccagcgat
     3781 ctgccccgtg acaaacagca ggcggcagag agaatcaccg ccggcctgat gaacctgatg
     3841 cagcagggcg tgatccgcag ccggcaggac gtggtgacgc agctggaaag ctacggcctg
     3901 accgtagcgc gggaaacgaa aagcagcatc agcattgccg atccggacgg cggccggaat
     3961 atccggctga aaggaatgat ttatgagcga gattttaaat ttggcgaagg gcttcgagga
     4021 gaaatcgaag cagcaggcgc aggataccga gcagagcgtg aagcgcgagt tcgcgaggct
     4081 ggagacgtct atcagcgcgg aactgcaatc aagctcgcag agcatcagca acgctatccg
     4141 cgagcagaac ggcaggctga ctggcatgat cagaacctcc gtttcagcgg cgatggcgtg
     4201 ggtattcctc tgcttcgccc tgttgatcgg catcatgggg ggaataatct ggtttcaggg
     4261 cacgatcatc acctcgcgcc tgagcgaaat ggacagctac agccagcagc tggcgaacct
     4321 gaaagcgaaa agcggcgcgg gcgtgacgat ttacagcgac gacacgcgcc ggaacaccta
     4381 cctggtagtg ctgccgaagc aggcgcacgg cgtggaaacc tacacgtcga cgaccgacaa
     4441 taccgtggtg aaatacacag cgaaataacc ccggcagagc gccctgaaac gccacagcgc
     4501 gtttttaaca ctgaggacga gcaaaaccat gaccgagatg gaaaaacagc ttctgagcgc
     4561 tttagagagc ttacagacaa gctacgagca acagcagcag gcgtggcagg acagctacag
     4621 cagcttgcag cacatgttcg aggctacctc gcaggcgtta acgcacagcg acagggtgtg
     4681 tcagcacttg agcagtcagg tggacagctt gcgcgcgcag gtcgagagct tgagcaggaa
     4741 tgtcagccgc ttgatgcgct gatcaggcag catgaccgcg cctacgccga gaagctgcgt
     4801 gagcagcagc cagagccgca gaaaaaaccg acccgctact atggtccctc tatgtgacga
     4861 cttttccgga atcagaatta agtgtactat gtactccatt gaaaacagga gacagccgtg
     4921 agtcacgcga ttgagtttat cgagaccccg atgtttaccc gtcagataaa acagatcgcc
     4981 acggatgaag aactcaggga gctgcaaaag acgctgattg agtcgccgga caaaggcgat
     5041 ctcattcaga aaaccggcgg gctgcgcaaa atcaggatgg cgacgggcaa ccagggaaaa
     5101 agcggcagcg tgagggtgat ttactttctg gccaccgccg aggtgatcta tctggtgatg
     5161 gcctacccga aaagcaccaa agacagcctg acggacgcgg aaaaagccgc gctgaagaca
     5221 ctgacccaac aactgaaaga cgaggtgtga catgagtttt tttgacgagc tgaaagcatc
     5281 gctggaagaa gccgttgaaa tcaaaaatgg cgtcagagag cctgcccgcg tcacccgtta
     5341 cgagctggca gacgtgaaag ccatcagggc acagctgaac gtatctcaga gcgagatggc
     5401 aaaagtgctg ggaaccagcc tcgacaccat caagagctgg gaaaccggac gccgtaaccc
     5461 gaccggactg gcggcgaagg tactggcaac catccaggcg aacccgaaat tcttccagga
     5521 actggcggcg cactaagccg ttgtgacaga aggattcgac aggcgaggcc gttcgcgtgt
     5581 aatttaacgg caacagaaaa aacaaagccc cggaaatcat gtcatatctt ggcggatcgc
     5641 atgataacca gggccaggtc gaaaccttga aaggatatta gcacatgaaa cgtgccagac
     5701 aacagcccgc cataagcgca gggcgaattg aataacgcag tactcgaact tttcaacgac
     5761 cgcttaccgc acaagccgta cttttcggac gatctgcact ttggtgtacg cattgccggt
     5821 aaggaacgcg ccattctggc gaaatacatc cagttcaatc agcctcacgc catgttctgg
     5881 ctgggattcg acgtcgatcg cgccggtgcc gctattgact ggagcgaccg caatgccccc
     5941 gcgccgacac tgacgatcac caaccccgac aacggacacg cgcacctgct gtacgcgctg
     6001 aaaacctctg tacgtaccgc gccggacgga aaaatgaagc cgctgcgcta cgccgccgcc
     6061 gtggagaatg cgctacgtaa aaaattagag gctgatgcgg ggtattcggg actaatctgc
     6121 aaaaatccga acca
//
