LOCUS       NZ_LR890275            12424 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain MINF_7C isolate MINF_7C plasmid 6, complete
            sequence.
ACCESSION   NZ_LR890275
VERSION     NZ_LR890275.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357519
            Assembly: GCF_904863235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:14:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,338
            CDSs (total)                      :: 5,207
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 62 of 196
            Pseudo Genes (incomplete)         :: 139 of 196
            Pseudo Genes (internal stop)      :: 26 of 196
            Pseudo Genes (multiple problems)  :: 31 of 196
            Pseudo Genes (short protein)      :: 4 of 196
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890275.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MINF_7C"
                     /isolate="MINF_7C"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="6"
                     /geo_loc_name="Australia"
                     /collection_date="2014-02"
     gene            complement(join(12282..12424,1..562))
                     /locus_tag="JMW01_RS26345"
     CDS             complement(join(12282..12424,1..562))
                     /locus_tag="JMW01_RS26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            705..1565
                     /locus_tag="JMW01_RS26350"
     CDS             705..1565
                     /locus_tag="JMW01_RS26350"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(1796..2632)
                     /locus_tag="JMW01_RS26355"
     CDS             complement(1796..2632)
                     /locus_tag="JMW01_RS26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480972.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(2633..3051)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(2636..3435)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(3496..4311)
                     /gene="sul2"
                     /locus_tag="JMW01_RS26365"
     ARG             complement(3500..4311)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4641..>4727
                     /locus_tag="JMW01_RS26850"
                     /pseudo=""
     CDS             4641..>4727
                     /locus_tag="JMW01_RS26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /name="Arm DNA-binding domain-containing protein"
     gene            complement(4999..6003)
                     /locus_tag="JMW01_RS26375"
     CDS             complement(4999..6003)
                     /locus_tag="JMW01_RS26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="WP_000427619.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     LLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
                     /name="IS110-like element IS5075 family transposase"
     gene            complement(6082..9048)
                     /locus_tag="JMW01_RS26380"
     CDS             complement(6082..9048)
                     /locus_tag="JMW01_RS26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_001138070.1"
                     /translation="MPRRSILSAAERESLLALPDTKDELIRHYTFSETDLSIIRQRRGP
                     ANRLGFAVQLCYLRFPGVILGVDEPPFPPLLKLVADQLKVSVESWDEYGQREQTRREHL
                     VELQTVFGFQPFTMGHYRQAVQLLTEMALQTDKGIVLASTLIEHLRQQSVILPALNAVE
                     RASAEAITRANRRIYDALAEPLSDAHRRRLDDLLKRRDNGKTTWLAWLRQSPVKPNSRH
                     MLEHIERLKAWQALDLPSGIERSVHQNRLLKIAREGGQMTPADLAKFEAQRRYATLVAL
                     AIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIGQALIEA
                     KQAGRDPFAAIEAVMSWDAFAESVTEAQKLAQPEDFDFLHRIGESYATLRRYAPEFLAV
                     LKLRAAPAAKDVLDAIEVLRGMNSDNARKVPADAPTEFIKPRWQKLVMTDTGIDRRYYE
                     LCALSEMKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASELPLAVATDCNRYLN
                     DRLTLLETQLATVNRMATANELPDAIITESGLKITPLDAAVPDTAQALIDQTAMILPHV
                     KITELLLEVDEWTGFTRHFAHLKSGDPAKDKNLLLTTILADAINLGLTKMAESCPGTTY
                     AKLAWLQAWHIRDETYGAALADLVNAQFRHPFAEHWGDGTTSSSDGQNFRTGSKAESTG
                     HINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAH
                     WDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYL
                     ERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
                     /name="Tn3 family transposase"
     gene            complement(9051..>9364)
                     /locus_tag="JMW01_RS26385"
                     /pseudo=""
     CDS             complement(9051..>9364)
                     /locus_tag="JMW01_RS26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9418..10122)
                     /locus_tag="JMW01_RS26390"
     CDS             complement(9418..10122)
                     /locus_tag="JMW01_RS26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(10192..10665)
                     /gene="dfrA14"
                     /locus_tag="JMW01_RS26395"
     ARG             complement(10196..10665)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            10821..11834
                     /gene="intI1"
                     /locus_tag="JMW01_RS26400"
     CDS             10821..11834
                     /gene="intI1"
                     /locus_tag="JMW01_RS26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_000845048.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     ARG             complement(1800..2632)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             706..1562
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ccagcgtggc gccgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc
       61 ggtacttaat ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag
      121 caagcgcgcg accataggcg ggcgctttat ccgtgttgat gaatcgcggg atctgccact
      181 tcttcacgtt gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg
      241 aggagagata aaaatcgaca gtgcggcccc ggctgtcgac ggcccggtac agatacgccc
      301 agcggccatt gaccttcacg taggtttcat ccatgtgcca cgggcaaaga tcggaagggt
      361 tacgccagta ccagcgcagc cgtttttcca tttcaggcgc ataacgctga acccagcggt
      421 aaatcgtgga gtgatcgaca ttcactccgc gttcagccag catctcctgc agctcacggt
      481 aactgatgcc gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat
      541 gccggccttt gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc
      601 accaccgact atttgcaaca gtgccctaaa tacattgaaa tatgtatccg ctcatgagac
      661 aataaccctg gtaaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      721 ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      901 tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt atcccgtgtt gacgccgggc
      961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1081 ccatgagtga taacactgct gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     1261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1681 gtgagttttc gttccactga gcgtcagacc ccgaatccta cgctaaggct ttggccagcg
     1741 atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt gatatctagt
     1801 atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt
     1861 ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg tcgagcaggc
     1921 gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca atgcgtctag
     1981 gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg gcgccaaagc
     2041 ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg gagaacatga
     2101 tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg ctcatcattt
     2161 gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt tgatcatcgc
     2221 gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca gaaggcaggg
     2281 gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt
     2341 agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat tcgagcaaca
     2401 tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc cataccagat
     2461 agtcggcccc gcgcagttca tcagcaatgt cttttatagg tttcaatccc ttgacgattg
     2521 caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga
     2581 gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac atcaacccca
     2641 agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg cttcgatccc
     2701 caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg cccagttctc
     2761 ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc cgagccgccc
     2821 aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt tcggcatgca
     2881 gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca ccggtagctc
     2941 tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt ccggtaagaa
     3001 gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca ttcgcgacag
     3061 cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa gttgctgccc
     3121 cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg gtactcccgg
     3181 aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga tgacctcggg
     3241 gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag cgagctcacc
     3301 gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc gaaaaacaaa
     3361 atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgatttac caaaaaaaat
     3421 attagttcga ttcaatggag gttccttcag ttttctgatg aagcgcgaat atagagaaat
     3481 atcccgaatg tgcagttaac gaattcttgc ggtttctttc agcgccgcca ataccgccag
     3541 cccgtcgcgc aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc
     3601 ggcaagctct gcagcgagtg tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc
     3661 gcgcagaaag gatttgcgcg aaacagacag aagcaccggc aaatcgaagc gcagccgcaa
     3721 ttcatcgaac cgcgccagca ccgagagcga ggtttcggga gcagccccca gaaaaaaccc
     3781 catgccggga tcaaggacaa ggcggttgcg tttgataccg gcacccgtca gcgccgcgat
     3841 gcgcgcgtca aagaacgccg caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg
     3901 atctgcctgc ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg atttcgccaa
     3961 ttgcggatag aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg ccacaccacg
     4021 cgacaaggca taggcttgcg tcgcgggttg ataactgtcg agcgagacgg gaatgccatc
     4081 tgccttgagc gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg
     4141 cgcggcgtcg ggattgctgg atgccggacc gaggtcgatc acatctgccc cctcggccat
     4201 cagcttacgc gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc catcggagaa
     4261 actgtccgag gttatgttga cgatgccgaa aatgatgagc gatttattca tgggggcttc
     4321 tataataata ataatcgagc atgagtctca tacggatgct cgggtcgaaa gggaatcccc
     4381 aggcgagtaa cctgtttgcg gtgatccatt agctgcagga gcagaatagc atacatctgg
     4441 aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc
     4501 aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga
     4561 aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt gggtatcgga
     4621 gacgaagaag gacaaggtac atgctgaccg ataccaagct gcgcaatctc aagcccaggg
     4681 acaaactcta caaagtgaat gaccgggaag gtctctatgt gggggtcgcc tcagaaaacg
     4741 gaatctatgg tcactcccgt ttttgcaaca ccgattttga cgacaagttg gcttgcttga
     4801 atctatccgg cgtctgaatg ggattttatt cccgcgcctc gatgagttcc gcgcctgatg
     4861 aacctccaga aaatatacgg cttcaatgag cctttccgtt ttacaggttc ctcaacaggc
     4921 cggtgggccg ttagtatcat caatatcagt attcgcaaaa ccagatgaat gattgtttaa
     4981 actggtgtat ttctgccttt atgcttcgta agtttgctgt cgcgccgtca gtgcccaggc
     5041 tattctggcc agcttgtttg ccagagcaca ggtgacgaca aagttgcttt tccgacacaa
     5101 caactccctg acccagtcgg ccaacttgcc agactggtgt tccagttttt gtatgaatac
     5161 cctggcacac tgaaccaaca aagttcggat ctttttgttg ccccgcttgc taatccctaa
     5221 caatgtcgtc cgacctcccg tgctgtactg tcggggtacc agccctgttg ccgccgcaaa
     5281 gtcacggctg ctggcgtact gcttcccgtc gccaatctca gttgaaatag tactggcagt
     5341 cagcgttcca acgcagggaa tactcagcaa gcgctgtcca acctcatctt cgtccaactt
     5401 tcgtttcaac tgagattcca gatctttaat ctgctcaaca agatagtgat aatgctgttg
     5461 taatttcagc agtaactggc tgagataaag aggcaaacta ctgtcctcaa gaagggtact
     5521 cagtcgacta ataacggcag cacctcgcgg aacgctgata ccaaattcca gcagaaaagc
     5581 atgcatctga ttagttgttt tcaccttatc ctgaaccagg gattcacgga cacgatgcag
     5641 agctcgcatt gcctgctgag attcggttct gggctgcacg aaacgcatag atggacgtga
     5701 tgctgcttca cagatagctt cagcatcaac gaagtcattt ttgttgcttt taacgaatgg
     5761 gcggacaaat tgcggtgata tcagctttgg aaaatgccct aactcttcca gcttgcgtgc
     5821 cataaagtga gaaccgccac aggcttccat cgcgatggtt gttgccgggc atgtcgccag
     5881 aaattcgatt agctttggtc gggtgaattt tttacggtaa acggccttcc cacgatgatc
     5941 ctgacaatga atatggaaag agttcttacc cagatcgata ccaataagcg caatgttttc
     6001 catgatggtt ctccgaatga aagcctgtcc tcagcatagt actgggaagg agggagtgac
     6061 catctcatta aataaagcac gctaagccgg ttgcagcggt cgtagcggcc tgaacttgcc
     6121 cgcgccgatc ttggcgctgc tgcgccagag gtaatcgccg gtgaggttga tgtgctccca
     6181 accgagcggc gacaggtact gcaacagcgc gtcatcaacg gcatggccgt tgccacgcag
     6241 cgcgtgcgca gcccgttcca gatagaccgt gttccacaac acgacggcag ccgttaccag
     6301 attgaggccg ctagcccggt agcgctgctg ctcgaaactg cggtcgcgga tttcacccag
     6361 gcggttgaaa aacactgccc tggccagcgc attgcgcgcc tcgcccttgt tcaggccggc
     6421 atgcacgcgg cggcgcagtt ccacgctttg cagccagtcc aggatgaaca gcgtgcgctc
     6481 gatgcggccc agctcgcgga gcgccacggc caggccgttc tggcgtgggt agctgccgag
     6541 ctttcggagc atcagggagg ccgtcaccgt gccctgcttg atcgaggtgg ccagccgcag
     6601 gatttcgtcc caatgggcgc ggacgtgctt gatgttgagc gtgccgccga tcatgggttt
     6661 cagcgcgtca taggcggcgt cgcccttcgg gatgtagagc ttggtgtcgc ccaggtcgcg
     6721 gatgcgcggc gcgaagcgga agcccaggag gtgcatcagg gcgaagacgt gatcggtgaa
     6781 gcccgccgtg tcggtgtaat gctcctcgat ccgcaagtcg gactcgtggt acagcaggcc
     6841 gtcgagcacg taggtcgaat cgcgcacgcc gacgttcacg accttggtgt gaaatggcgc
     6901 gtactggtca gaaatgtggg tgtagaacgt ccgccctggg ctgctcccgt atttcgggtt
     6961 gatgtggccg gtgctctcgg ccttgctgcc ggtgcggaag ttctggccgt ccgacgatga
     7021 ggtggtgccg tcgccccagt gctcggcgaa gggatggcgg aactgtgcgt tgaccagatc
     7081 ggccagcgcc gccccgtagg tttcgtcgcg gatgtgccag gcttgcagcc aagccagctt
     7141 ggcgtaggtc gtgccggggc aagactccgc catcttggtc aggcccaggt tgatcgcgtc
     7201 ggcgaggatc gtggtcagca acaggttctt gtctttggcc gggtcgcccg atttcagatg
     7261 cgcgaaatgc cgagtgaagc ccgtccattc gtccacctcc agcagcagtt cggtgatctt
     7321 gacgtgcggc aggatcattg ccgtctggtc gatcagcgct tgggcggtgt cgggtaccgc
     7381 cgcgtcgagc ggcgtgatct tcaagcctga ctcggtgatg atggcgtccg gcagctcgtt
     7441 ggccgtcgcc atacggttga cggtggcaag ctgtgtttcc agcagcgtca gccggtcgtt
     7501 caggtaccgg ttgcagtcgg tggccacggc cagcggcaat tcgctggcct gcttgaggct
     7561 ggcgaatttc gcgggcggca ccaggtagtc ctcgaagtcc ttgaactggc gcgacccctg
     7621 cacccagatg tcgccggaac gcaacgcgtt cttcatctcc gacagcgcgc acagttcgta
     7681 gtagcgccgg tcgatgccgg tgtcggtcat gaccagcttc tgccagcgcg gcttgatgaa
     7741 ctcggtcggc gcgtcggcgg gcaccttgcg ggcgttgtcg ctgttcatgc cgcgcagcac
     7801 ctcgatggcg tcgagcacgt ccttcgcggc gggagcggcc cgcagcttga gcacggcaag
     7861 gaattccggc gcgtagcggc gcagcgtggc gtagctttcg ccgatgcggt gcaggaaatc
     7921 gaagtcctcg ggctgcgcaa gcttctgcgc ttcggtgacg ctctcggcga aggcatccca
     7981 ggacatgacg gcctcgatgg cggcgaacgg atcgcggccc gcctgcttgg cctcgatcag
     8041 cgcctggccg atgcggccga acagccgcac cttggcgttg atcgccttgc cggacgcctg
     8101 gaattgctgc tgatgcttgt tcttggcggc gttgaacagc ttgcccagga tgcggtcgtg
     8161 caggtcgatg atttcgtcgg tgacggtggc catgccctcg atggcaagcg ccaccagggt
     8221 ggcatagcgt cgctgcgcct cgaacttggc caggtcggcg ggcgtcatct ggccaccctc
     8281 acgggcgatc ttgagcaggc ggttctggtg caccgaccgc tcgatgccag aaggcaggtc
     8341 gagcgcctgc cacgctttga ggcgttcgat gtgttccagc atgtgccgcg aattcggttt
     8401 gacgggcgat tggcgcagcc aggccagcca ggtcgttttg ccgttgtccc gacgcttgag
     8461 cagatcgtcg aggcggcggc gatgcgcgtc cgacagcggt tcggccaagg catcgtagat
     8521 gcgccggttg gcgcgggtga ttgcttcggc gctcgcccgc tcgacggcgt tgagggcagg
     8581 cagaatgacc gactgctgcc gcaggtgctc gatcaaggtg ctggccagca cgatgccctt
     8641 gtcggtctgc aaggccatct cggtcagcaa ctggacggcc tgccggtagt ggcccatggt
     8701 aaagggctgg aagccgaaca ccgtttgcag ttcgaccagg tgctcgcgcc gggtctgctc
     8761 ccgctgcccg tattcgtccc agctttcgac gctgaccttg agctggtcgg cgaccagttt
     8821 caacaagggc ggaaacggcg gctcatcgac gcccaggatg acaccaggaa agcgcaggta
     8881 acagagctgc acggcgaagc ccagccggtt ggccgggccg cgccgctgcc ggatgatgga
     8941 gaggtcggtt tcgctgaacg tgtagtgacg gatcaactca tccttggtgt ccggcaacgc
     9001 cagcaggctt tcgcgctcgg cggcggacag gattgaacga cgtggcatat ttactgatcc
     9061 gttctcaagt attgatacag ggtttcgcga ctgattccga attcacgagc cagcttggtc
     9121 ttttgctcgc cagcctcgac acgttggcgc agttcggcaa tacgctcaga cgacagggat
     9181 ttcttcctgc cacggtaggc cccgcgctgc ttggcgagcg cgatgccctc gcgctgccgc
     9241 tcgcggatca aggcgcgttc gaactcggcg aacgcgccca ttaccgacag cacaggttcg
     9301 ccatcggcga gtcctcgccg gtgaaggtca aatgctcctt aaggaactcg atgcgtacgc
     9361 cgcgcggcac tgttgcaaag ttagcgatga ggcagccttt tgtcttattc aaaggcctta
     9421 catttcaaaa actctgctta ccaggcgcat ttcgcccagg ggatcaccat aataaaatgc
     9481 tgaggcctgg cctttgcgta gtgcacgcat cacctcaata cctttgatgg tggcgtaagc
     9541 cgtcttcatg gatttaaatc ccagcgtggc gccgattatc cgtttcagtt tgccatgatc
     9601 gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt tcaacgtcag acgggcaccg
     9661 gccttcgcgt ttgagcagag caagcgcgcg accataggcg ggcgctttat ccgtgttgat
     9721 gaatcgcggg atctgccact tcttcacgtt gttgaggatt ttacccagaa accggtatgc
     9781 agctttgctg ttacgacggg aggagagata aaaatcgaca gtgcggcccc ggctgtcgac
     9841 ggcccggtac agatacgccc agcggccatt gaccttcacg taggtttcat ccatgtgcca
     9901 cgggcaaaga tcggaagggt tacgccagta ccagcgcagc cgtttttcca tttcaggcgc
     9961 ataacgctga acccagcggt aaatcgtgga gtgatcgaca ttcactccgc gttcagccag
    10021 catctcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
    10081 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
    10141 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgcttt gttaaccctt
    10201 tttccaaatt tgatagcaat agttaatgtt tgaagtaaag tgttgctcaa aaacaacttc
    10261 gaaggtattt ggaatactcg ggaagaaaac atccccctct ggctcgatgt cgatcgtcga
    10321 taagtggagc gtagaggcca tgggtaatgt ttctcggtaa atttctccgc caccagacac
    10381 tataacgtga ccggtgaatt cagctagcct gtccatggcc tcttcgattg actgaaatac
    10441 aactacattg tcatcatttg atgtccaacc tgagcgggta acgaccgcgt atttcctatt
    10501 ggggagtgcg cccatagatt caaacgtctt gcgacccacc agaagccact gattgtaggt
    10561 caatgcttta aaaagtagct gctccccttt cgcggaccag ggtatgtctg gaccgcaacc
    10621 aatcacgccg tttttcgctt tcgcagccat caatgatact ttcaaggttc tcatcctggg
    10681 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
    10741 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
    10801 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
    10861 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
    10921 aacaggctta tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
    10981 caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg
    11041 tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc
    11101 tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc
    11161 cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc
    11221 gtttgttcgc ccagcttctg tatggaacgg gcatgcggat cagtgagggt ttgcaactgc
    11281 gggtcaagga tctggatttc gatcacggca cgatcatcgt gcgggagggc aagggctcca
    11341 aggatcgggc cttgatgtta cccgagagct tggcacccag cctgcgcgag cagctgtcgc
    11401 gtgcacgggc atggtggctg aaggaccagg ccgagggccg cagcggcgtt gcgcttcccg
    11461 acgcccttga gcggaagtat ccgcgcgccg ggcattcctg gccgtggttc tgggtttttg
    11521 cgcagcacac gcattcgacc gatccacgga gcggtgtcgt gcgtcgccat cacatgtatg
    11581 accagacctt tcagcgcgcc ttcaaacgtg ccgtagaaca agcaggcatc acgaagcccg
    11641 ccacaccgca caccctccgc cactcgttcg cgacggcctt gctccgcagc ggttacgaca
    11701 ttcgaaccgt gcaggatctg ctcggccatt ccgacgtctc tacgacgatg atttacacgc
    11761 atgtgctgaa agttggcggt gccggagtgc gctcaccgct tgatgcgctg ccgcccctca
    11821 ctagtgagag gtagggcagc gcaagtcaat cctggcggat tcactacccc tgcgcgaagg
    11881 ccatcggtgc cgcatcgaac ggccggttgc ggaaagtcct ccctgcgtcc gctgatggcc
    11941 ggcagcagcc cgtcgttgcc tgatggatcc aacccctccg ctgctatagt gcagtcggct
    12001 tctgacgttc agtgcagccg tcttctgaaa acgacaaaca gccagaaagg ctgttacagg
    12061 cgatttgatc tgcaacctat tggttaaatt aatgtatcaa aaacgatggt ttctgtgaca
    12121 catccctata agcagttgcc tatacaataa aacgctctcc ggctgtaaca aaacccgtta
    12181 cagtatcaat acaacaaaaa cgatgtaaca aaatagggtg gcaaaatgag gcactgttgc
    12241 aaagttagcg atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg
    12301 cttaccaggc gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg
    12361 cgtagtgcac gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta
    12421 aatc
//
