LOCUS       NZ_LR890232             4064 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae isolate KSB1_6J plasmid 3, complete sequence.
ACCESSION   NZ_LR890232
VERSION     NZ_LR890232.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06112204
            Assembly: GCF_904863225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:10:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,335
            CDSs (total)                      :: 5,219
            Genes (coding)                    :: 5,083
            CDSs (with protein)               :: 5,083
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 58 of 136
            Pseudo Genes (incomplete)         :: 84 of 136
            Pseudo Genes (internal stop)      :: 21 of 136
            Pseudo Genes (multiple problems)  :: 24 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890232.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KSB1_6J"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="3"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8457 S 144.9821 E"
                     /collection_date="2014"
                     /collected_by="University of Melbourne"
     gene            join(3872..4064,1..1067)
                     /gene="mobQ"
                     /locus_tag="JMW15_RS26450"
     CDS             join(3872..4064,1..1067)
                     /gene="mobQ"
                     /locus_tag="JMW15_RS26450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_099745348.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="mobQ"
     gene            1376..2233
                     /locus_tag="JMW15_RS26455"
     CDS             1376..2233
                     /locus_tag="JMW15_RS26455"
                     /inference="COORDINATES: protein motif:HMM:NF015076.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_099745352.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication initiation protein"
     gene            2230..2499
                     /locus_tag="JMW15_RS26460"
     CDS             2230..2499
                     /locus_tag="JMW15_RS26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_099745351.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(2514..2780)
                     /locus_tag="JMW15_RS26465"
     CDS             complement(2514..2780)
                     /locus_tag="JMW15_RS26465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519627.1"
                     /translation="MSQKKEQKTAEKPTAGPLRKHRSRAEKIDDLKKQISELRKAEREE
                     RQRARDEKLIILAKAVYEKAGKPKDIEALIDFVKSEDFKIEYK"
                     /name="hypothetical protein"
     gene            complement(2913..3191)
                     /gene="traD"
                     /locus_tag="JMW15_RS26470"
     CDS             complement(2913..3191)
                     /gene="traD"
                     /locus_tag="JMW15_RS26470"
                     /inference="COORDINATES: protein motif:HMM:NF018152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_099745350.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="traD"
     gene            complement(3181..3612)
                     /locus_tag="JMW15_RS26475"
     CDS             complement(3181..3612)
                     /locus_tag="JMW15_RS26475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157769352.1"
                     /translation="MNKKQALWIGGKMNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNE
                     DVFNRCLKLYYAIDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNY
                     INIYELKKLFLLQKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     Typing          4..1064
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgattaaatc aagcaatgat ctatggaact atgttgaaaa aacagagaag agaaaggacg
       61 caagaacagc aaaagaattt aaaataactt tgccttgtga acttaccaat gaacaaaata
      121 taaaactatt gaaagaatac ttaacaaaaa actttgttag taaaggcatc atttgcgact
      181 ttgttataca taacgacaaa gacaataaaa acccgcacgc tcatgtgatg attacgacaa
      241 gagaaataac gctttcagga tttggtaaaa aagtaaggtc atgggatgaa gaaaaaaccc
      301 ttcatgattg gcgtaaacaa tgggcaaaag ttcaaaatca gcatttgaaa aatgcaggct
      361 taaaatctcg cgtcagtcat agaacactag aagaacaaaa aaatataatg atcgatttag
      421 ctaaaaaagc agaagatgca aaagattttg acaagtctat tttatatcta gcaaaagcta
      481 ttgaactaga tcgcccacca atgcagaact tatcacgtaa aaaatggaga acaaaagaag
      541 gtcaagaaca acgcaaacgt gaccaagcta tcagagacag ggcgagagcc gacgctaggg
      601 ctttcaggtt gttgagaggt gttgcagttg ttgatgttgc ttcattcgtg gttcatccgt
      661 tagagaaaaa agagcagtca aaaaataatg acttttcaga aaaaaaatct tggtggcgtt
      721 ctactgttaa ttttataaaa aatattaaag ctaagttctc aactaaaaaa gatattaaaa
      781 acgatgtcca tgcatcagac gatttaatga ctgatccggt gacgggttta ccaatcacca
      841 aagcgaaatg gcaagagatg agtgacaggg caaacgtaag gactccacag cctgcgatct
      901 catatcaggt taagcaatcg gacggggaag ggctaaaaac ggctcagaat gcgttacagg
      961 acgttttaga cgtatcagag ggtaaatcgt taaaggtgaa gtctgtgccg gttccgggtt
     1021 ctcgtaagcg tcgagttgtc gaggaagaca gcagtgacaa cggttagcta accagcacag
     1081 ccagatccta cataagatta cagggcttcg ctcatagcct acggcctgta cctatcaggc
     1141 tatagcttaa cttgtgatga tttgaagcct tcggcctgtg cttcgcaaaa atacccccag
     1201 acccattatt acctgatatg gctaaaatga ccacttgaca cttttttagt ggtgactact
     1261 tgacaccatg aaaggttatg ttagaacagt aatataaaaa caaaaatccc tgatttccga
     1321 atgtttggcg acaggagaaa atcagggatc tacaataaaa ggattgtatt aaaaaatgat
     1381 actacaaaaa gaatatagca gaagtgattt tataaatcaa attatatcaa gaccattttg
     1441 cactgacgac ttcaaaagag atggtattta tagaacaaac aaaagacgag ctttaaacag
     1501 cgtttatatc gagcataaca acgatagctt cattaactct attgtgtttg atattgacag
     1561 cgatacagcg gctattgcat ggcaagatgc agatataccg aagcctaact ttataactca
     1621 aaatccggct aatggtcacg ctcatttatt ttatgcctta tcatctcctg tttgtataac
     1681 agaaaacgcc agaagaaagc cacaaaagtt attaaaaggc gttatagaag gactcaccga
     1741 aagattaggt gcagatcctt gttatacggg caagataaca aaaaaccctc tcaatccgag
     1801 atggcgtaca ttttggaacg aacaaccaag atttgaattg aactatctat gtgagttcat
     1861 agatacaaat aaaagagtaa aaaaagaagt aagaaaatca atagttgcag agggaaggaa
     1921 tacagcgtta tttgatagtc tgcgttttta cgcttacagc ataattttta aatatcaaaa
     1981 aaatgatgat ttctgcggtt tcatttcagc attacaagaa gaagctgaaa acataaacga
     2041 ttcattcgaa gatcaactaa gctttaaaga aataaaccac acaataagaa gcatttcatc
     2101 ttgggtatgg gggacttttg atagtgatac tttcagcgaa atacaaagca acagagcaaa
     2161 gaaagcgaaa ggtaaaacta aaaccaatac aaaagcaaaa aaacttatag aatttatagg
     2221 agtaaaaaaa tgaaaggcaa cgagataaga aagggcttaa cacaaaaaga gatggctaaa
     2281 aagttcaatc tttctttaag cactgtaaaa aaatacactt ctcaaccaag agaacattat
     2341 gaatcagaag cagaaaacag aagaaaaaca gcttatacgt tacaccataa aaaaggctta
     2401 aaatggcgtg aagtagctga aaaaatgaat acaacagaaa atgccgttaa agcattagcc
     2461 aaaagataca aacaaatcga ccaagaaaac aggggctaaa atgcccctta gctttattta
     2521 tattcaatct tgaaatcttc agactttacg aaatcaatta atgcttcaat atcttttggt
     2581 tttcctgcct tttcataaac agcttttgca agaataatta atttttcgtc tcttgctcgt
     2641 tgtcgttctt ctctttctgc ttttcttagt tcgctaatct gcttttttaa atcgtctatt
     2701 ttttcagctc tagatctgtg tttacgaagt ggccctgcag ttggtttttc tgctgttttt
     2761 tgctcttttt tttgagacat tttttactct cccggatgat atgtttttta aacatatcac
     2821 aaagccgggc tacggtgaag actaacgcac aagccccgcc agcggcgggt tcttgcttgt
     2881 tcaacccttc caagtgtcag attagaaatc tgtcacttgt cagcgttttc tagacgtgat
     2941 tttagcaatc tttcaccatt gttttttaac gcttgttttt gctcatcgct taacttgtta
     3001 aagtgttcaa gaaaacccac tacaagagct agatcgtaat ctaaaccaaa cacccttgta
     3061 acttcaccgc caacagtaaa ttttttatgt tctctagctt ttctttcttt ctgcgatgct
     3121 tcttttttaa gtttagataa tctatcttgt gcaagtgcca ttctttgctc taacgttttt
     3181 ttacttccca tactcaacct ctcttttaat tttttctatc tctgaaatca tacttttaaa
     3241 atcaaaatca ttgatatttt tttgtaataa aaacaacttt ttcagttcat agatatttat
     3301 gtaattattt agtgttaaat acacaagatc ataatcagtt ataattggtt catcatgatc
     3361 tttattttca cgatctttat ttatcaaata tatcaacgct tcaaacaatg attttttatt
     3421 gttatagatt gcatcatcga tagcgtaata cagctttaaa cacctattaa aaacatcttc
     3481 attaacttcg tcattgatga aatcgttttt aatatcttca atgtcgatct cgcttgtaaa
     3541 aatcaatagc cttttttcta tttctttttt attcatttta ccaccaatcc ataatgcttg
     3601 ttttttattc aatatcgtaa cttttaaata ttgtcaaatt tgactttaat ttaatcagag
     3661 ttaatttata tatatcagtg aaagaaaagc tagtttgttt ttcattcctc ttttagtttg
     3721 agcgttagcg aaaaataaaa gctaaggaat gcccggttac gcattgtaaa caatgccggg
     3781 gtctgccgac ggctaaaacc tgtttttctt acgaaaaact aaaacccaaa cccaaaagaa
     3841 aaaatagggg ttctcatgag gatataatta tatggcttta tatagattag aaatgcagaa
     3901 cgttagtcgt tcggatggtg tcagctcagt tgcaaaagct gcttatcgtc atcgttccgt
     3961 catgatagat gaacgaaccg gagaaataca cggcgagaaa tcagctaatc gtgacgatct
     4021 ggtttatgct gaaatattgg caccagaagg aacgccagat tttt
//
