LOCUS       NZ_LR890212             3681 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae isolate INF022-sc-2279895 plasmid 4, complete
            sequence.
ACCESSION   NZ_LR890212
VERSION     NZ_LR890212.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3356968
            Assembly: GCF_904863055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,391
            Genes (coding)                    :: 5,167
            CDSs (with protein)               :: 5,167
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 69 of 224
            Pseudo Genes (incomplete)         :: 154 of 224
            Pseudo Genes (internal stop)      :: 40 of 224
            Pseudo Genes (multiple problems)  :: 31 of 224
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR890212.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="INF022-sc-2279895"
                     /db_xref="taxon:573"
                     /plasmid="4"
     gene            192..353
                     /locus_tag="JMW18_RS27825"
     CDS             192..353
                     /locus_tag="JMW18_RS27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            complement(511..666)
                     /locus_tag="JMW18_RS27310"
     CDS             complement(511..666)
                     /locus_tag="JMW18_RS27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(774..1031)
                     /locus_tag="JMW18_RS27315"
     CDS             complement(774..1031)
                     /locus_tag="JMW18_RS27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(1033..>1524)
                     /locus_tag="JMW18_RS27320"
                     /pseudo=""
     CDS             complement(1033..>1524)
                     /locus_tag="JMW18_RS27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            1562..2107
                     /locus_tag="JMW18_RS27325"
     CDS             1562..2107
                     /locus_tag="JMW18_RS27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048289822.1"
                     /translation="MPLPERMKPARVTRKQMKELISQLNGILDHIDNGMDENNEELKQM
                     MADWNAKVVMPCGFSDFRDFSSWTSALDFTRMALNQEKYLDDFTWKELNDVINFIRKAE
                     GSASDHSYALQLLEINFKANPLDLIYHPDCWFNDEALFHARLTTEEIGGYLMKKSGRWL
                     NDAPDIQLVYAIPQDVYD"
                     /name="hypothetical protein"
     gene            complement(2855..3277)
                     /locus_tag="JMW18_RS27330"
     CDS             complement(2855..3277)
                     /locus_tag="JMW18_RS27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3277..3468)
                     /locus_tag="JMW18_RS27335"
     CDS             complement(3277..3468)
                     /locus_tag="JMW18_RS27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          11..508
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.227"
                     /coverage="99.0"
                     /evalue="1.74e-142"
     Typing          3517..3681
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.114"
                     /coverage="92.0"
                     /evalue="4.34e-24"
ORIGIN
        1 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga
       61 tggctccctc gtgcgctctc ctgttcctgc cattcggttt accggtgtca ttccgctgtt
      121 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
      181 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgtctta tccggtaact
      241 atcatcttga gtccaacccg gaaagacacg acaaagcgcc actggcagca gccactggta
      301 acggatttag aggagttggt cttgaagtca tgcgcctgtt aaggctaaac tgaaagaaca
      361 agttttggtg actgcgctcc tccaagccag ttaccttggt tcaatcacct gcaagtgttt
      421 gaaccttcgg aaaaaccgcc ctgcaaggcg gttttttcgt ttacagagca agagattacg
      481 acgatccaaa tgggatctca agaagatcct ttacggattg ccgttcgtac cgctatcaag
      541 cgtgactccg gtcaccgtcg atgaaggtga aacagcccca cctccggtgt ctcgaacatt
      601 attgacctga catgctgcca gaaccacaga aacggtaaca acccctagga aaagtaattt
      661 tttcatatat caccttttat ttttattatt ctaggcaaat ggatatctag taattattct
      721 tcaaatccag agttacaaat gagcgggctc ataacttgaa cccgcaaatt caatcacgct
      781 tttttaaaac ctggcttacc gttagcagct cgccattcct ttacggtttt aacaattcct
      841 ttcgccgaag aatcttcgcc taccttaggc ttgaagatga ggttccaacc agcaggatgc
      901 tctgttagtt gggcgaacga ataaatagca tcatcgttct ctttgtcagt tggaaaatca
      961 gcagcgatta ttttactaac aaaagcagta aattcttctt ccgtaaaatc tgaaatttta
     1021 ttaccagcca ttttattttc ctttatgtgt ctcaatatga tttttaggtg ttagcgcctt
     1081 aatgttatcg acatccataa cagcccctcc ttgagatatt tctttttcgt ggtgtaattc
     1141 aattttaaca cgtttcccta ctgattctaa aaagtccaca gtaggtgccc ttccagcttt
     1201 catctgatta atatcagctt cactaaattg cttgctcagt gcagaatcgt cagcaaccgc
     1261 tttccagaac gcccgacgga aagaatcaaa actaccgaaa gtcttcccgc gtaatttatc
     1321 cgcaatctgg gaaggaacag gagcaccatc cccggtactg gcccccccca tccagttatt
     1381 gccaacgttc tgccctttgc cgctcactgt accgggcatg ttacgagggc tacggtacat
     1441 cacatacagc ggttttaggc cggattccgg aggaaacacc agaataatat cgtcaaaatc
     1501 gaggtcatcc gctagattac tcagttcccc cgagtgcacg ataaaaaaga gagtatttct
     1561 gatgccatta cctgagcgaa tgaaacccgc acgggtcacc cgtaaacaga tgaaagaact
     1621 gatcagtcag ctgaatggca ttctcgatca catcgataac gggatggacg aaaacaatga
     1681 agaactaaaa cagatgatgg cagactggaa tgcaaaggtc gtcatgccct gcgggttctc
     1741 tgattttcgt gatttttcat cctggacaag tgcactagat ttcaccagga tggcgcttaa
     1801 tcaggaaaaa tacctcgatg actttacatg gaaagaactg aacgacgtta tcaacttcat
     1861 acgcaaagca gaaggtagtg catctgacca cagctatgca ctccagttac tggagattaa
     1921 tttcaaggca aacccactgg atctcatcta tcaccctgac tgctggttca atgatgaagc
     1981 attgtttcat gcccggctga ctaccgaaga aattggtggc tacttaatga aaaaatcagg
     2041 tcgatggctc aacgatgcac cggatatcca actggtgtat gccatccccc aggatgttta
     2101 tgattaacca agtcaccatt taccataatc ccttaattgt acgcaccgct aaaacggcct
     2161 caggacgttt ctgacgggaa accggtaaaa caggcaacaa accacccgaa aacccgccag
     2221 aaacgcgcag gctctgtttt tacggcatgc atgagtatgc gcgcaagtgc atagcgcgga
     2281 aattcacgcc attcagccag ggtaagtcct gtttccggcg ttcttctgat ccgaaaacgg
     2341 tgaacgggga cgcaatttat tgcgtggccg tgaggcgttg ccggcgcagc cggaaaaccg
     2401 gttttcagca cgttcaggaa tcgggttcgt cgacggccgg caatcacgtt atggcaagtc
     2461 gccggacgac tcaccacacg attcaccggc gattcgaatc gtgaattgaa tcaagagtca
     2521 ccatgaacaa aaaacgccca aattgatgaa ttgaatcaag agtcgattca tcataacaac
     2581 atgataataa agcgtgattc acatatgaat cgtgggcaat tcgtaaacga ttcgccatag
     2641 agtcgtccac gacttggatc tgcactttgt aacacatcac tgcacattaa tttttcatta
     2701 accaattgtt ttaaataagg aatctgcaaa attttaattt tgcgtagtgt gtgggtattt
     2761 ttagtcgtga ggagcgttca gagatctggc gatttttggg aaggttcagg aactccctgg
     2821 gatggccgga gaggaaaact caagcatggc aggatcagga ttttttccag aatttccacc
     2881 acggggaatg gggtggtgaa gaaggctcgc gcttgtgctc cagaagctgc atagcctgac
     2941 gaagggaatc aatatgctca tctcgagcct taacagcccc tttaagacca tcgttttcag
     3001 cttcaagtag agcgattcta tgttgtaaat catgtaaacc aacgctgtta atacttgtag
     3061 attctgcgtc actttttaca tcatatttta cagtatcgat gacaccatca agagaaccaa
     3121 acactctaat aagctctgaa gtatcaataa ctttaatagc cttcccgtgc tttacaacag
     3181 aggtgtaact taacttccca ctgttaatca tcttgtaaat tgtgggacgg ctaaccttaa
     3241 caagccgtgc cgcttcacta atagacactc tggccatcaa ttaccatcct gtaattcatc
     3301 cagtctcgag gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag
     3361 gtcggcctca acatcgagat caagctcgtt aagcttctcc agcaggagca gagactgatt
     3421 ctggatgaat ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa agggcctcag
     3481 agcgtcagtg ctcaatgtca gacagatctc ttttccgctt cctcgctcac tcagtcgcta
     3541 cgctcctgcc gttcggctgc ggcgagcggt cgcaacgcgc acataacgac atagagcaga
     3601 cagctcggag agggagaagg cagcacagcg gccgtttttc cataggctcc gcccccctga
     3661 caagcatcac agaatttgac g
//
