LOCUS       NZ_LR736240            13569 bp    DNA     linear   CON 14-FEB-2024
DEFINITION  Staphylococcus epidermidis isolate BPH0662 plasmid 4.
ACCESSION   NZ_LR736240
VERSION     NZ_LR736240.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3982825
            Assembly: GCF_900086615.2
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Lee,J., Stinear,T., Seemann,T., Kwong,J., Buultjens,A., Baines,S.,
            Carter,G., Monk,I., Vandelannoote,K. and Goncalves Da Silva,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2019) UNIVERSITY OF MELBOURNE, Doherty Centre for
            Applied Microbial Genomics, Doherty Institute for Infection and
            Immunity, 792 Elizabeth St, Melbourne, VIC, 3000, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 01:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,806
            CDSs (total)                      :: 2,725
            Genes (coding)                    :: 2,646
            CDSs (with protein)               :: 2,646
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 45 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 25 of 79
            Pseudo Genes (multiple problems)  :: 28 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR736240.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /isolate="BPH0662"
                     /db_xref="taxon:1282"
                     /plasmid="4"
     gene            1..936
                     /locus_tag="BN6763_RS00005"
     CDS             1..936
                     /locus_tag="BN6763_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(1161..1400)
                     /locus_tag="BN6763_RS00010"
     CDS             complement(1161..1400)
                     /locus_tag="BN6763_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1417..1686)
                     /locus_tag="BN6763_RS00015"
     CDS             complement(1417..1686)
                     /locus_tag="BN6763_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            1896..3818
                     /locus_tag="BN6763_RS00020"
     CDS             1896..3818
                     /locus_tag="BN6763_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            4092..4505
                     /locus_tag="BN6763_RS14745"
     CDS             4092..4505
                     /locus_tag="BN6763_RS14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            4582..4815
                     /locus_tag="BN6763_RS00030"
     CDS             4582..4815
                     /locus_tag="BN6763_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(4864..5568)
                     /locus_tag="BN6763_RS00035"
     CDS             complement(4864..5568)
                     /locus_tag="BN6763_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5719..6417)
                     /locus_tag="BN6763_RS00040"
     CDS             complement(5719..6417)
                     /locus_tag="BN6763_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            6581..6679
                     /locus_tag="BN6763_RS00045"
     CDS             6581..6679
                     /locus_tag="BN6763_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6856..7767)
                     /locus_tag="BN6763_RS00050"
     CDS             complement(6856..7767)
                     /locus_tag="BN6763_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(8186..8746)
                     /locus_tag="BN6763_RS00055"
     CDS             complement(8186..8746)
                     /locus_tag="BN6763_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(8997..9563)
                     /gene="qacR"
                     /locus_tag="BN6763_RS00060"
     ARG             complement(9001..9563)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9741..11285
                     /gene="qac"
                     /locus_tag="BN6763_RS00065"
     CDS             9741..11285
                     /gene="qac"
                     /locus_tag="BN6763_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_155974661.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIAASIGGVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            11490..12050
                     /locus_tag="BN6763_RS00070"
     CDS             11490..12050
                     /locus_tag="BN6763_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(12369..13130)
                     /locus_tag="BN6763_RS00080"
     CDS             complement(12369..13130)
                     /locus_tag="BN6763_RS00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             9742..11282
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.61"
                     /coverage="100.0"
     Typing          2..936
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1897..3749
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgcccaatt ttgaaaaata taatttatca caagtaaaaa ctgaaagatt ttatcaactg
       61 cctaaatatt tatttgaaga tgcatatttt aagaaaatgt ctgcagaagc caaaattatg
      121 tatgcgttat taaaagatcg ttttgaatta tccctccaaa atgaatgggt agataaaaat
      181 aataatattt actttatttt cagtaataaa catttgtgtg aatacttagg ttatgcagaa
      241 caaaaaatta taaaattaaa aaaagagtta ataaaattta atttactaac tcaagaacgt
      301 gttggcctta ataaaccaaa tagattatac ctattaaaac ctaattatga cattgaagcc
      361 agtcatatca aggaacttcc aaattcacag ttccagaaca atgaatttgg aagttctaga
      421 actgtgaatt taagtggtca agaacttcca aattcacagt ctaatgatac tgattataat
      481 gacactgatt atattaagac taattataat gatatgtatg atttgaatga taggaaatta
      541 acatattcta gtaatcatac aaatcattca aatcactact attcaaaatt caatgatgaa
      601 gctttaaaat ttcaattact tgaagaacta ccacaaagta ttcaaagtta tttaagtaac
      661 ttttctgtat ctgaaattaa acttattaaa tctgtgttat taaaagccaa aacatccttc
      721 aacaattcta ttgatgcata ttatttatta gaagatatgg aatttgaaat tgttaatgtt
      781 cttaagcgtt ttaaagctac attaattcaa aaaaatgaaa ccgttgaagc aatgcaaggg
      841 tacttaatga aatctcttaa atctgaattt gcagaagtac atacgcttaa taaacgacgt
      901 gatcatttac caatcacttc tttatttaat caataataca acttaataag ccaacaatga
      961 tccaattaga gaatatcttt aattagatgt tctcttaact gtaagcgaac tttattttca
     1021 atgaaatgta attaattaag taagtgggac taaaactttt cttttttagt cccattttta
     1081 taaacaatta ttattcattg ttactactat cagcaatttt ttctttttag tgaaacccaa
     1141 gtatttgtaa gcatgataaa ttacttcttt ttatatttat tgggtttatt tgtattaatg
     1201 tagttggaat acatctttaa caattcttct gtttcatcag gacttaaatg ctcacattca
     1261 aaatactttt caagcattgc accttttcga atcagtctat gtgctctttc ttttctatct
     1321 aaatttttta atctatgctt tcttaattct gctttttcaa tttcagcatt tatttttttg
     1381 agatgtttaa aatcattcat cgtatcactt actattctag ttaattttgt cttctgttaa
     1441 attttccttg ttctcgttgc tgctattttg ttcgatatct ttattaaatt ctccaatgac
     1501 tttatccata attttataca attcatcact agagttgatc atatttaaat caagtttatt
     1561 taaaaaataa ccaccaattt tattagatag ttgttcttct tgttttttct tttgctcttt
     1621 cagttgtgca atttgttttt ctaattgact taattttgta atatctgttt gtttaatttt
     1681 agacattaaa aaactccctt agtttatcta tataaagcat accataattc attgggtaag
     1741 tcgcacatcc atggtataat ataaccagtt cttaagacac tgcactggcc catttacatg
     1801 caaattgaaa attggcataa aaatgggcaa ccatactggt tgatcattat agttcccaca
     1861 ggggtaaaaa aacagaaaag agaggaggta aaatcatgaa taaaggttac tttatgttga
     1921 gagtgactaa cattaatcga gcaacaaaaa atatcgttgc gtcagcgtct tatcgttctg
     1981 atgaagcatt atatagcgaa cgtacagatg aaaaaattaa gtttagaaat catactatta
     2041 agcctgaaag catgatttta actcctcaac atgcacccga atggactaaa gatagacagc
     2101 gtttatggaa tgaagttgat aaagttgaaa agaataatgc gaaaacaaaa aatcctagat
     2161 tagcaaaaga agtcttatta agtttgccta atgattttga tagagaggta caaactgaat
     2221 taactaaaga ttttataaaa agtgagtttg tagacaaagg aatggttgct gatatttcaa
     2281 ttcatagaga tgatataaac aacccacacg cccatgttct acttactcaa agaccgttta
     2341 atgcagacgg tacttggggg aaaaagacaa aaacgagaac gcaatatgat gaaaatggca
     2401 atgcgatttt aaataaaaat ggtaataaag ttagaaaaca agaacgattt gccgactttg
     2461 atttcaaaga agttcgtaag cattgggaat taaaattaaa tcaatactca gaacgtgaac
     2521 aaagtttaag acgatatgat agtcgttctt ttgaagcgca aggtttagaa aaaattgccg
     2581 aaattccact tacaagagaa gaatatagat tagaacaaaa tgaaagaaaa cgctgtgaaa
     2641 aagagggtat cgagtaccaa cccgttactt attacggaca gaaaaatgaa caaattagaa
     2701 gatataatcg tggcgaagta tcacaattat ttagtgataa agagaaagaa caagcacaaa
     2761 atgaaattag attatttatt caaaatgcta acgaacaagt aagtaaaaat gaacaaagtt
     2821 attctatttt atttgataga tatcaacgtg atgttggcta ttcagaggcg aaagaaacaa
     2881 ttaaaaatac gtttgacagt gcgtctacct ttggtcgtaa aattcaaaat gatttactta
     2941 aaaatgaaat taagagacgt caattaaaat tctatgctaa aaattatgat gaaaattatc
     3001 agtttagaaa agaaattgaa agtaaaggat atagtcaaca agagtttgtt aatactgttg
     3061 aaactaacta taaagcgtta ctcaatgaga agaataaatt agaaagtaga gaggaaaaac
     3121 gtaaagagat ttatcaatca gctaaagatg tttatctaac tgaaagtgaa aaaaatcatc
     3181 tcattgttca gcaaatgtac cctaaatctc atcatcattt tagccatgat gaaaaagcat
     3241 ttattgttga tgaggcacaa aaaggtaata ttataaaagc aactcatgtt caatacctgt
     3301 tcaaacaaaa taacaatatt cctaatgatg tattgctaga agatacttat aaaaaagcga
     3361 gtaaagatat tttctttagt caaaaacatc ttaaaaattt agaaaaagat agctttgaat
     3421 ataacgttga gttgcaattt attaaggaaa aagaaaaaga aattgatagt tataaagaac
     3481 atattgatga tgatttaaga atagatatag gcgataaaaa ctttgaaaaa gtcaaagacc
     3541 aaacacctaa tgaaaaagtt caattactca ttaaacttga gaaatataat catgtcgatc
     3601 aagaagaagt tatcgaaaaa catattaatc aagtagatag aaatgatcat gacaatcgta
     3661 ttgaacatga aaatgaacaa actaatcaca ttaatttacc aacgataaat gctagtgact
     3721 ttcttatgta ctatgcgaac gaaactgaaa agcaaaacaa tattaatcat cagaaaaaga
     3781 agaagaaaca agagagaaat aaccaaatga gaagatagta agcagttgaa atcatacaaa
     3841 gttcattctt taataataag aaacttaacc caatgtggta aattagcttt ggtttaaaga
     3901 aaacatttat atacttagat aaggtacatt ctatgtatta tcacaatgta ttttaaaacg
     3961 tgtataatta tagaaaaacc ttgatattat gcgattgttc acatatttca gcataaaaaa
     4021 tactaatgtt cataaatatc gatagaaaaa attgagttta aatatatctt aaagttaagg
     4081 agaaaaataa tttggaaaca aaaattggac atcaaataca aaagttaaga aaaagtaaaa
     4141 acatgtctca agaaaaatta gctgaaaagt taggggtatc gagacattct atttctaatt
     4201 gggaaagaga agtaagtaat cccgacttaa aaacaatttt agaaatcact aaattattta
     4261 atgtttctct aaatcaatta attaaaggag tggaaatcat gcaagttaat aaatatgttt
     4321 actctgcatt agcatttttt ctaggaggtt tcggggctca taagttttat gttaagaaaa
     4381 ataaaaatgc tcttctttat ttactatttt gttggacggg aataccgggt gtgataggta
     4441 tggtagaagg tgttttaaca cttatgcgtc cgagcgatag tgaaaacaat atagaaataa
     4501 attaaaccaa aaataaaaag ccgatgtatc ggattttttt tagtgtacat gtgaatatta
     4561 aaaatgatgg agtgatttca catgaacaca ttcgttttcg gcgtgattac aaagcgatta
     4621 gaagaaatag gttttaatgt acacaaggtg tttaaaatgc ccgaaaaaca ggcactattt
     4681 gaagatttgt accattatgt gagatacgtt aaaccatgca aggctgaact aacaatggtg
     4741 gcattagata gtgatgaatt gattcattac tttatatgct gttacatgga acgttatgct
     4801 tatcttttga actaatgata aaaaagacgc ctaattttag gcgccttttt aataatctac
     4861 taactactta ttatatctaa ttttacttgc taacttagct actgttttat tgtcgtaggg
     4921 tttaatatag actgtttcaa tattctcctc tattgctttt tttgttatct tattcatttg
     4981 actataatca ctattttgtg aattttccca tttataaggg atattattat atacattaat
     5041 tgttccatca ccattactgc tatatgttac agacccacca gctctcatag gagcttctaa
     5101 tttcacaact ttttctttat aaattgcact atttttggct tgtggattta cttttgagcc
     5161 ttttggtatt tctgtaacat aaacattttt aatacctttt aaatcatttc ttgtagatat
     5221 taactgatac caaactcgtg catattcaat ttctttcgag ttaaaattat ttaagtaact
     5281 tttattttta tcagtagatt gttgcttctt attactatca ttattttttt cttgtttttt
     5341 attatcattt ttttctttat tttgtttatt actactttct gaattatccg cactatcaga
     5401 tgtgttagtt ttttcgtcaa tttcatttga tgcttcatta tctttgtttt ctgaggtgtt
     5461 agtatcacta gaattattat cattgtgatg ttctgaatcg ttattattac aactcactaa
     5521 aattaccgat aaactaacaa ttaatatagg taaaatgatt tttttcatat tacaactcct
     5581 tttcagtatt tcaaattaga ttatgttaat attttttcag caagaaaacg taaattacat
     5641 gagttgaaaa atatgtatta aatatataat aaaataacca catgttttac ataaacacgg
     5701 tggttatttt atcaattttt atattgcctt ttttctgaat attaaaagac ttatacctaa
     5761 tattagtagt ccagaagcgc caattattga caatatatca ctttggttgc tttctcctgt
     5821 ttctggtaat acttgagagt ttgaatcttg atttttcatt ccttcagaac tcttgttttt
     5881 attttcagtt acaggtgtca acacaccttg aggtgtatta atagttttat tttcttcatt
     5941 agctatcttt tgactttcat tttttacaat ttgattatat ttttctgtgg atacactatt
     6001 agggttagga acatgacttt ctaattcttg gtattcttct tgagtcatac aattaggtat
     6061 cacccctccg aaaggtcttt taccttgttg agttctctca ttattttgta tatcagtact
     6121 ttgtgattga tattcattaa atacacttga aactttctca ttatactcga tttgagttaa
     6181 ttcccctgaa tctttctgtt tagaaagttc acttatttga ttttgtgcag attgtgggag
     6241 ggatgaagta ttgaagtcta cattagccca accatctcca tttgtatctg cgctatttgg
     6301 taagttctct ccgtatacgg ttttggcacc atcctcagca ttggctgtcg ctgaaaaacc
     6361 tgtgaatgtt aatgagctag ccaataataa agttggaagt aatgatcgtt ttttcataaa
     6421 attatctcct ttaatagaat ttgtattttc attatcacat agatacatta aacataaaat
     6481 gacttatcgg aagtgcatat gcttaacaat tcacaaatga tataatataa aagtcgtcgt
     6541 tctacttctg tgctggttta gcatggaagg aggtgacacc atgttcagca tcatatttgt
     6601 atctatagta gctcctatta tagtaggggt tataatcacg ttgttttcac attggttgaa
     6661 taatcgtgat aaacaataaa gcgacgacac agtaataaca ccaaaaagag cccgataact
     6721 actggaatag ttatcgggct cggtgtttac catgttcaac acatatttgt tatctaaatt
     6781 ataacatcat agtgtggagg aatgcaaaat tgaataatta gtatgttaag tctatactat
     6841 actgattagt aagtactaat tatcatcttt gttattgtta cccctttcat tggcttttga
     6901 atctaattct ttttgaaatt ttttgtgtga ttttgaagaa gtgtttaaaa agaaataggt
     6961 catattatcc atacgatgtc cttttataaa ttcattgata aattgttttt ttgtcatttt
     7021 tttggttgta ttacgttgtt tttgcaatct aatgatttct tcttcaatgt atactcttat
     7081 catcaaatct aaactagatg tctgattata agtagtgaat aattttggaa ctaataaagt
     7141 tttattacca tcaaaaagaa atggagtgaa atcataagtt tcccatatac cgttagtaga
     7201 gaaataataa tatgtcacag tttctttaga aagaaacgat ttcatttctg gaaatgactg
     7261 aatcatggat ttattaaaat caattataac attcatgatt atattacttg ttaaatctga
     7321 taatttatca tcaccaaaat ttctgattaa tggtgctttt tgaggtattg ttaatccgtc
     7381 atcatttaag ataatggaaa gaatatcttc attcataaat agattaatca attcagatgc
     7441 tgtagtacct tttcctgtat tacctttctg agaaaaacct aagcgtaaat ggtgtggttc
     7501 tgaaaaatga gagaataatt ttctaaattc attacgcttt tgggtatcag aaaaatcact
     7561 aagatgtata tcgataattt tttcaaaaaa aacatcaatt tgctcttgtg ctctttgagc
     7621 caaagtcgca acttttccaa cttctctatt agatattact ttaataaggt ttgggtctat
     7681 atattgaaga gtgtcttctt caatagaaat atttaaataa tcagttgatt tagcatcatg
     7741 attgaatttg gtacatacta gtgtcactct atatcatcct tctgtaagtt ttttgtttac
     7801 ttccaatata gaatgatgta ttattgtaat tgtcgattta caataagttg ttattctagg
     7861 tggaagttta ttgcttttga tgtatagata gacttaacct tttcattata gcatactata
     7921 gaagaaaatg caagcaaaag catataaaca acaaatgttg tttatatgct tttgcttttt
     7981 tgttaattgg agaggacaag gtgaatatta acagtcaaaa tccagttatg tcggttaatg
     8041 aatcgatgaa aattttcaaa gctcaagtaa tgcttagaaa tttaaagcta ttaagcgagt
     8101 ggattgttaa aggggactga agttaaagac actttttaag aagatttttc aattaaaggg
     8161 tatattaaaa ctttaattta gtactttata tttttcttga ctctttaata atcctatata
     8221 cagtagaacg actcactcct gtttgttcag caatttcttc accagacagc ttttgttcat
     8281 catataagaa tttgatctct cttttcttat gatctggtag tgaaggtctt ccaccttttc
     8341 tccctcttgc tcttgcagct tccaaacctt ttttagttcg ttcacttaat aaattagctt
     8401 caagttcagc gaatgcactc atcatggtaa agaacatctt gcccatcgca tctttcgttg
     8461 acacattcat atctataata tgtaaatcaa tgctattttc atccaaccat tgagatagct
     8521 caattaattg ttttgttgta cgtccaagac gatctaattt atagacaact agaatatctc
     8581 cttcacgtaa ataatctaaa cacttatcaa gttccgttct tttagtttta cgaccactcg
     8641 ctttctcgct aaagatacgt tcacaaccat attcctcaag tgtatcaatc tgtccatcaa
     8701 gactttgatc ttgtgttgac acacgtgcat aaccaatttt agccatttga acaccacctt
     8761 ttttaatcgt tttatgggta cttactaatg aaatatagtt ctcgatgtaa ataaacacgt
     8821 ctgtcagcat ctaaaaactc atgattttcg atatttatga tacattgatt ataacacaag
     8881 tttatagact taaatatcca agaaaaacct cttaatatta agttaagagg ttttgtgtca
     8941 aaaacgaacg taatttagaa ataatattta ttggtatttc aagaatatat atataattat
     9001 ttactaagtc catttaaaaa aatttgagag aacttgttca taagtttaat tctttcatta
     9061 atattttgtt catgtgtaaa ggtaacaatt ccattaacag cgtttgctgc tattttacta
     9121 acagcattaa catcattaat acaccattcg ccatttaaat taccctcctt aaaaattaca
     9181 tgataagcat ctatatattt gttttctaat ttattcattt tttcattaat gctattagtt
     9241 ttataatatt cagtgtaaaa ttcaattatt gcgttttgaa gtggataata gtattcggtt
     9301 gttaaagata gttcattata taaataaaat ttttctctat tagttttaca tttgatttgt
     9361 tcctttttcc actgttcttg ccatttagat tcttctatat ttaaaatttc taaaaataga
     9421 ttttcttttg ttttaaagtg ataataaaga ttccctttac tactttctga taatttaaca
     9481 atttctccag tagtagtggc attatatcca ttttttataa ataattcctt tgcgacacct
     9541 agtattttat ctttcaagtt catcacgcct ttaatgagta ttaagtacta tcaatgatag
     9601 cacaaaaaat tcaaagaata attgtataac acaatacagc catttaaatt tcgcaagatt
     9661 ttttgttgta atatgtaaaa aaatagatta taatccttat agaccgatcg aacggtctat
     9721 aaggattgga gggaacttaa atgatttcat tttttacaaa aactactgat atgatgacat
     9781 caaaaaaaag atggactgca ctagtagtat tagctgttag tttgtttgtt gttacaatgg
     9841 atatgacaat attaattatg gctttaccgg aattagtaag agagttagag ccttctggta
     9901 cccaacagtt atggatagtt gatatatact ctcttgtttt agctggcttt ataattccat
     9961 tgagtgcctt tgctgataaa tggggaagaa aaaaagcatt attaactgga tttgctttat
    10021 ttggcctcgt ttcattagct atatttttcg cagaaagtgc agagttcgta atagctattc
    10081 gatttttact tggtattgca ggtgctttaa taatgccaac tactctttca atgataagag
    10141 taatttttga aaaccctaaa gaaagggcca ctgcattagc tgtatggtca atcgctgcat
    10201 cgataggtgg tgtttttgga ccaattatcg gaggagcttt acttgagcaa ttttcatggc
    10261 actcggcatt tttaattaat gtaccgtttg cgataatagc agttgtagca ggtttatttt
    10321 tattaccaga gtctaagtta tcaaaagaaa agtctcactc gtgggatatt ccttctacaa
    10381 ttttatcaat tgcaggcatg attggactgg tatggagtat caaagaattt tcaaaagaag
    10441 gactagcaga tattattcca tgggttgtaa tagtattagc aattaccatg atagtgatat
    10501 ttgttaaacg taatttatca agttctgatc caatgttaga cgtaagactt tttaaaaaga
    10561 gatcattttc agctggtaca attgctgcat ttatgacaat gtttgcaatg gcatctgttt
    10621 tgttattagc ttcacaatgg ttacaggttg tggaagaact ttctcctttt aaagctggct
    10681 tatacctatt acctatggca ataggagata tggtgtttgc accaattgca cccggattag
    10741 cggcgcgatt tggaccgaaa atagtgttac cttccggaat tggaattgca gccattggca
    10801 tgtttattat gtatttcttt ggtcatccat tatcatattc tacaatggct ttagcattaa
    10861 ttttagttgg agctggtatg gcttcactag cagttgcatc tgctctaata atgttagaaa
    10921 cacctacatc aaaagcaggt aatgcagctg ctgttgaaga gtctatgtat gaccttggaa
    10981 atgtttttgg tgtagcagta cttggtagcc tatcttctat gctttatcgt gtatttttag
    11041 atatttcatc tttttcatca aaaggtatag ttggagattt agctcatgta gctgaagaat
    11101 ctgtagtggg cgctgtcgaa gtagctaaag ctacggggat aaaacagctt gcaaacgagg
    11161 ctgtaacatc atttaatgat gcttttgtag caactgcttt agtaggtggg attatcatga
    11221 ttatcatttc aatagttgtc tatttgttaa ttcccaaatc acttgatata actaaacaaa
    11281 aatagtacta aacatatatt tttagatatt gtagattagt ttatttttaa aattagtaat
    11341 agttaaaaag tagaaccatt aattttaaat ggttctactt ttttattatt aagtaaaatt
    11401 ataaaagaca atttcttaaa ttaataatat gatataccta tatctaataa tttattagta
    11461 ttaataaacc ttgaaaggat gattaactga tgaatagagc agatattttt aaaatatttg
    11521 atgtagaact taatataact atatttttag gagcactagt agcaactttc atctatttta
    11581 taagttacat gaacaatgta aatgaattaa ttacattatg cttaacatta gcttttcctg
    11641 catatttatt aaaaattagc agatcgttcg aggttatttt attagaggaa aaaataaaaa
    11701 atagtaaaat caaaatctgt gacaaagata gaattgaacg agagatactg gtgaaaaaga
    11761 acctaataga tgaactattg tttcctaata gagttattaa aaaatataca ctgacaattt
    11821 taatttacag tataggtatt attattcttt tactactttg taatatgtta agttatggaa
    11881 ttggtgaaaa tgtcaatttt atctggagat tactagcttg ttatgtaacg agtgtcacag
    11941 tgattatagt tgccttgatt ataatgataa tgatatttga tcttataatt ggattactat
    12001 taaattttca aatattttta aaaaataaaa agtatgatga ctttttataa ttattcgatt
    12061 tatataatga aaaattaaat gattaaacat agaaacctca aaacaaacca tatttaatag
    12121 agtttatggc gccttataca aataaaggct atgctgttaa agaattatgt agctatcttc
    12181 agattaatat ggatgatgtt tatactattg gcgatagaaa attgatttac ctatgtttaa
    12241 taccctagat aattcttctg ataatttagt ttttgttaga gtttaacata actgatgatt
    12301 atcagaagtt tgattcacat taaaaaggca caacaagttc atgaacttgt gtgccttttt
    12361 aattgcaatt agttaccaaa taaacgactc caaaatctct ttttaggtgg ttctacctcc
    12421 ctattatcac tggaatcgtt cttcgttttt tcaacaaact tatcctcttt tgttaattca
    12481 tctgtactgt catgcttatt ctcttcagtt atatttttat gatttacttc ggttggttga
    12541 cattcttttt gttgttggtt gatattttga gattcttcag taaaagttgc ttcttgtgga
    12601 ttaatattct gtctattatt catcgttgta tcaaaagaat atttaagctg tctttcttct
    12661 tctaattggc tttctagttt ttgaattttt ttattgtttt ctaaagataa tatttgttga
    12721 ttctccaaca gtttatttaa tgtattaaca ttgctatttt gttgatctaa ttgtttagtt
    12781 agattctcaa tatatttttc atcattttta gctctagttt caaagatttc tatttgcttt
    12841 tctaattcgt ttacttttgt ttttaatgtc tcagaactat ttgaattatt atgtatatac
    12901 ttttcaactt ttgcagtatt ttcattaaat aattcatttt ttactgattc tttattatga
    12961 gttgttttct ctttcttagt gattttttct ataattttta aataatcatt atctttaata
    13021 taattaattc cattttcttt ttcaaaagat atatttaatc ttttagcatt attaacaata
    13081 gtttgtttag ttacacctaa ttcatcagca atcattttaa tagttttcat aacatccccc
    13141 ttaggtacct aatttagtac ctaaatttta ccacctaaca aaatttattt catcatttag
    13201 tactaaattt actatctatt aactacctaa atgcctaatt atctatcctt aaaatatttt
    13261 cgccacttaa ttaggtgcta aaatagcaaa ttatacgttt ggtagtttta ggtgtacttt
    13321 taattacatt taaaactctt tatatacgcc attaaaagtg ttaatattac tttataaata
    13381 ttaaaagagt cgatgctatt ggcgtagcat cgactctcgg taataaaacg attcgcactc
    13441 gtttgtttat atattttttt gatacttgta ttatatatat ctaatcatct aagtgcaagc
    13501 acaaaacata taacttacgt aaaaattgtt ttattacctc aatcccaaaa tggaaatgag
    13561 gtttttatt
//
