LOCUS       NZ_LR595694             2088 bp    DNA     linear   CON 20-JUN-2024
DEFINITION  Escherichia coli isolate EcMAD1 plasmid pEcMAD3.
ACCESSION   NZ_LR595694
VERSION     NZ_LR595694.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4732083
            Assembly: GCF_901733115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Patino-Navarrete,R. and Patino Navarrete,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2019) INSTITUT PASTEUR, 28, Dr Roux, 75015, Paris,
            France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_901733115.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 02:32:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,789
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,428
            CDSs (with protein)               :: 4,428
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 78 of 244
            Pseudo Genes (incomplete)         :: 160 of 244
            Pseudo Genes (internal stop)      :: 50 of 244
            Pseudo Genes (multiple problems)  :: 36 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR595694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="EcMAD1"
                     /db_xref="taxon:562"
                     /plasmid="pEcMAD3"
     gene            complement(132..398)
                     /locus_tag="ECMAD3_RS24025"
                     /old_locus_tag="ECMAD3_00001"
     CDS             complement(132..398)
                     /locus_tag="ECMAD3_RS24025"
                     /old_locus_tag="ECMAD3_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(402..1367)
                     /locus_tag="ECMAD3_RS24030"
                     /old_locus_tag="ECMAD3_00002"
     CDS             complement(402..1367)
                     /locus_tag="ECMAD3_RS24030"
                     /old_locus_tag="ECMAD3_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(743..974)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ctatcctgca ttggtgtcga gttggcaagg ctgaggataa tgctgaggat aatgcaggat
       61 agacagagca ctagagattg catgcaatct cgtacctatc acctcagcct gccgctggct
      121 cttttccaga actaagaatg cgttcttaaa aatccaaact tgataccgtt taaatctatt
      181 ttcacaaaaa gagtttttac cctctcgttt gttttctgaa tatataaaat taattcatcc
      241 tcaaaacctt cgtgcggatg aaaaccaaat gtgccaaaac ttaatccatc aataagcaaa
      301 ataaggtttt taaacctgtc aggaatcacc gcttcacttc ttaaaaaaaa taaaccaata
      361 gtcttatttc cattcaggta ttcaatatta atattcatac gttaatccga tttatccttc
      421 tcaggggcgc gtttgtaacg ccgtttacct gaatcccaga caaacgccgt ccgcttcccg
      481 tcatcagtgc catcccctac atcgtcggca atggcaagat cctcattggt ttctcgatcc
      541 aactggagga cgttttttag cgctccaccg gtcgagataa aacggcgctt gtgaagctgc
      601 cgcgtcagct caagaaacca ctcaggatcg tttgccatat cttccggttt aacggagtat
      661 ttcagcgttt cagcaaccgc gctttgcagt tgctcggcaa cgttggccac aacctcacct
      721 gtcttagttt ttactgcccg aatatcgata ttcggctcat agttcacccg caagcaatcg
      781 cgccagagtt ctacccaacg ttcgtgctta acgtagttct tccctttaaa ccaagaaggt
      841 tgcaccatca gcagacagtg aaaatgcgga tgtgcgctgc catccttacc tcgcgtcacc
      901 tccgtagccc tgatccaccc ctgaacaggt gatagctcct ttcgcttttc cattcgctta
      961 aacgccgcat tcattgctgt aaggactgtt ccaagttcac ctatctcgca gttcctgaca
     1021 gttaacgtca gaaacaacca tcgggaagac gggtaatcca caacgatttt cggtagagcc
     1081 tgataaaaac gggcttgcca catgagggtt cttctccact ggcagacagg gcaatgacgg
     1141 acacggcaaa attgcgcact acgaagccgt aggcgcgttt ctccggtttc catgatggta
     1201 gaccaaccaa aacgtaacaa accgctacag gaggccatac ggagtgcata gcgctcgaat
     1261 tcagcagcag tctggtagat acccccgaca gaatcggtaa gcgtgcgctg ggtatcccaa
     1321 actgcatcac gggggctgta gtccgaaagg aatttatctt ctgacattaa gcctgctccg
     1381 atctgggcag gctgtgattt gatgtggtat attcatcacg tacctgtgca ttgcgagcca
     1441 gtacatgtca ccagagtttg ccgctgaggt gacacattcg aaatgttggg gagtgttgac
     1501 gcactcccca acgccgatta aaaacgcctc aattctgctc cattttcttt gacctgtcaa
     1561 aacccactca aagcctcgtc attactgggc taaaccacta tttcataccc gccatttatc
     1621 ccttaagtat aaatacaagg atcacgcgcg agaattgcgc aaaaaataac cacaatttta
     1681 accccccaaa acaagcaccc cgaacggctt accccaacct tttccacaca cccgatcacc
     1741 aaaacagggg ataaaaacaa aaccctgtct tacaggtgga tggcctcact tcgtgaaacc
     1801 atccaccccg cataagtttt ttaatcggtg gtgtcgtcaa tcactaaatc ggaaccccaa
     1861 aggggacccc cacgatttag catttgacga gattaactct cggagatggc atcatgcttt
     1921 taacggactt taaaaaacgg tgaacggatg cggattttaa tccgtgtctg tgaggcgttg
     1981 agcaacgagc aaagcgaggc ggtagaaaga gagccagcgg caggctcagg acgataatcc
     2041 ggggaaaatg taccactcgc agagtgttaa catttgacat cggattgt
//
