LOCUS       NZ_LR135496             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8414 plasmid 9.
ACCESSION   NZ_LR135496
VERSION     NZ_LR135496.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885787
            Assembly: GCF_900639715.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:15:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,505
            CDSs (total)                      :: 3,414
            Genes (coding)                    :: 3,291
            CDSs (with protein)               :: 3,291
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 42 of 123
            Pseudo Genes (incomplete)         :: 78 of 123
            Pseudo Genes (internal stop)      :: 26 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135496.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8414"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            167..253
                     /locus_tag="EQB57_RS18240"
     CDS             167..253
                     /locus_tag="EQB57_RS18240"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_125669474.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(555..779)
                     /locus_tag="EQB57_RS18245"
     CDS             complement(555..779)
                     /locus_tag="EQB57_RS18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1145..1429
                     /locus_tag="EQB57_RS18250"
     CDS             1145..1429
                     /locus_tag="EQB57_RS18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1432..1848
                     /locus_tag="EQB57_RS18255"
     CDS             1432..1848
                     /locus_tag="EQB57_RS18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1985..2704)
                     /locus_tag="EQB57_RS18260"
     CDS             complement(1985..2704)
                     /locus_tag="EQB57_RS18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     Typing          complement(1986..2704)
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agattcgata aaaactatgt ccttcgttct aaagcgcctt acaggcggtc agggcaagcc
       61 ctaacaaccc caaggcaata aaaaacttag gcttattttt gcttttttat tgtataattg
      121 attgtcgcct atccgacagt cttgcgactg gaaggaggtg tgtttcgtgt tcactaactt
      181 tatttgctcc cttcttgttg ggcttttaag cgctttgttc agttactggt tgaataaccg
      241 aaacaagaag taaaacttaa ttaaggcgac tcagcccaca aaaaaagccc cagagaatcc
      301 gacctctctg gggctttcgt gtgtttcgtg ttcacttatt tgcaatcaaa ttataacaga
      361 aataaatatt tatttcaaaa atatcctaca aaagtctaaa atccgtaatt atttcttata
      421 tgcaacattt acaaataaag tcgctctgag agctcgtatt tttcgattta agacgtttta
      481 tttagttttt ggtgtatttc attggtccaa gctaaaaaac tcttagaagc gattttagag
      541 ccatttaaac ccacttaatt atattctcca tgaaattcgc tccatgcgtc aaaagctgcg
      601 tctgaaattc cgccgtcttc gttcactagt tgcattaatt tttccaacga tcgttttctt
      661 tcgggtgtca tatttttata ttccaacaat ttttcgtctg ttaaatcgaa cttgattgct
      721 tgaattgctt gctctaattt ttctaagttt tgaaaatatt cgtaaggaaa tttttccatt
      781 ttttctttct ccagtctcac aatttttcta gaaataaaat cacttctttg ccgtctatac
      841 tctcaaaaaa aatgtcgttt gaaaaactaa ataaattttt gatcgtgcat ttttaaattt
      901 ggttatcgat tacacgtgca gcactccccc ccgaatttta aagagcataa ggaaacgtgt
      961 acccactttc taaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa
     1021 tttgatatca ctttttcgcc atcgtgggta gcttatgtat agcattttgg caaaaaacag
     1081 ctatacaaat atagtatttg tatggtatac ataaattata cataattgca aaaggagatt
     1141 ttttatggac attttagttc ggaatattga tccaaagttt gttgcaacaa tagacaaacg
     1201 ttgccaagaa ctcacgaata aaacaggaga aaaatggagt cgaaataaat atctaaaagt
     1261 tttaatcgaa agtgattttg accgtcaact tacgacttac aaaaaagacc aattcgattt
     1321 attgctcgat aaatttattg ctatccaaac agaaaatact caaatattaa gcgagtatgt
     1381 ctacacaaat aatcaaatca ttgaaatgct tatcggagga atgatataac catgagaaaa
     1441 acttctgtcc aaaaacatgt tactttgtac cctagtcatg ttgacgtttt agattccatc
     1501 atcgaaaaaa atccagatat caataactac tcggaagcca ttcgcttttt atgtatgagt
     1561 tatgaacccg ataccaacac gaccactacc agcaatcagg caaaactaaa tgctatggga
     1621 aaagaaattt ccatcatgac tgaaatcatc gcagaatttg ctgatttata tctaaaagat
     1681 acaggcgttc ttattggcac aaattctaaa gtatatcaag aagtgaaaga aaaaatcgaa
     1741 gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc atcaaaaaga aaccagtcaa
     1801 acagtcactt ctagaaattt cgacccaaca aattttctaa aacgataaaa aacagatagg
     1861 agttattcct atctgttttt tatttagaag ccccatacaa tatgctagaa tgtactagca
     1921 atcttagtcg gttgtaaggg acttgtctac ttgcttaatg taaggggggc gagttctttt
     1981 ttatttattc ttctaaccag ttacgcagag aaatctttgg caaatctgac gttaggcttg
     2041 aaaaagtaaa ttctaatttg gcgattttat tgcccttttt tgcttttatt tttgtgactt
     2101 taagatcatt gaagtatttt tttagttctc gtaatgcagg atttaatact ttttgatcaa
     2161 tgttacccat ttgatagtat tctgggatat ccattaagct cctaaactct tcaatacgaa
     2221 ctacataata accagtagaa cggtactgca taagcagtcg gaatagagtt tttgcatacg
     2281 tagaacgcaa cgacgtgaaa gcagataatt caaatctact aaattcagct gttaaaccat
     2341 taataatata ttctaggtcc ggattaacac tgacttcaac aaactttttt gttttattga
     2401 tttcaaaacc agtaaacaaa acaaagtagc gaatagaatc ctcatcttca gtacggtaag
     2461 taagattaag catatcttta tagagctttt ctaaatcacc gacaaaggct tttgtagcag
     2521 tcatcttata atcacttaac tcttttaatt cctcgaaaga aaatctaact tttttcaaac
     2581 cacgatcttt catacgagcg cataatgcaa aaaataaatc catctgctta gcattgaaat
     2641 ttttcagcgg tactaaattc aattcatttt gatatttgac tgttaattcc gttttttcat
     2701 ccattccttt cttcccccct tacaaataaa taataacctt taacaaccaa ctcgtaaata
     2761 ggttggttgt tattatacaa aaggatatta gtactcagat aaaaagttgt tagtactcag
     2821 ataaaaagtt gttagtactc agataaaaag ttgttagtac tcagataaaa agttgttagt
     2881 actcagataa aaagttgtta gtacccttct gtatccgttg ggagagtaag gctcaactgc
     2941 cccctaaaaa gggttctaaa aaatctttaa aaaataaata aaataaacgc gcgcgcgcga
     3001 gcaaaatttc ccgccaaaaa atcacccact ttttaaaata aacaggttcg cgcatgatat
     3061 taaactgcca atctagctcc ctatggtcgc tgtaagaagg agtgatcgct acgctctcac
     3121 attttacttg cagcagtcgc ctttaggctc cttcgccaaa aataattcat tagggactcc
     3181 tttgtggcgt cctgagagct cgtctaagcc gttctaagac a
//
