LOCUS       NZ_LR135495             4372 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8414 plasmid 8.
ACCESSION   NZ_LR135495
VERSION     NZ_LR135495.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885787
            Assembly: GCF_900639715.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:15:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,505
            CDSs (total)                      :: 3,414
            Genes (coding)                    :: 3,291
            CDSs (with protein)               :: 3,291
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 42 of 123
            Pseudo Genes (incomplete)         :: 78 of 123
            Pseudo Genes (internal stop)      :: 26 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135495.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8414"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            54..416
                     /locus_tag="EQB57_RS18205"
     CDS             54..416
                     /locus_tag="EQB57_RS18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045135959.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            638..2044
                     /locus_tag="EQB57_RS18210"
     CDS             638..2044
                     /locus_tag="EQB57_RS18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_060810507.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="plasmid recombination protein"
     gene            2489..3457
                     /locus_tag="EQB57_RS18220"
     CDS             2489..3457
                     /locus_tag="EQB57_RS18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002347008.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            3460..3693
                     /locus_tag="EQB57_RS18225"
     CDS             3460..3693
                     /locus_tag="EQB57_RS18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            3686..4105
                     /locus_tag="EQB57_RS18230"
     CDS             3686..4105
                     /locus_tag="EQB57_RS18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            4114..>4372
                     /locus_tag="EQB57_RS18235"
     CDS             4114..>4372
                     /locus_tag="EQB57_RS18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127822059.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHV"
                     /name="hypothetical protein"
     Typing          2581..3444
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.264"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          639..2041
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.094"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa ggagatgtag tttatgggaa
       61 tgactttagc agatattaaa gcgcaagaag aaaaattaaa aaaagaacaa gcgaaacttc
      121 gccaacagaa aaaacgcttg attgagaaag aaaatgctgc aatgggcaag gctcttcgca
      181 agcgttttgg gtttgaaact tttgctgatt ttgagcggtg gttatcgtca atttctgacg
      241 atcagcaaca cgaaaaaaca gtggataatg agtggcttgc tgatgaaaaa tcagcgccaa
      301 gcgaacgaga ttatcccgat gaatctgacg acttattcgg agtgataccg aataagaaaa
      361 gtcagatgag tgagagtaaa tttacgtcaa tatttaatga cgtgaattta cgataagcga
      421 ttattttgcg tgaagcaaaa taaagcataa aaggagttga tagcgtgata cgctttcggc
      481 tccttttttc gttgtcactt gtgacagcga aaaccctcgg agagcgtttg gaattgtgac
      541 atccatgtca caattaggaa aatgttactc tttgacaaag agtaacagaa aggtttacaa
      601 tctttttatt agcgacagcg cgaaggaggg ttttaacttg gggtttagta tttcatttaa
      661 aaaaggaacg aagaatacaa gtatttctca taataatcgt gagttaactg atcgccagaa
      721 aaccaatgat tggcataagc acattgactt cacgaaatcg gatgaaaata tttatttaga
      781 gcagacgaat ctagcagata aatatgagga attgtttggc gaagtggtag aaaaatataa
      841 tgcgaaacaa aaaagagccg atagaaaaat cgaggactat ttagcgaaag tgcgcaagga
      901 taaaaagttg gaacctcaac gagaatttat tgtgcaaata ggcattttag atgatttcca
      961 gacaaatcga gcagatggtt cttctactgg catatcgaaa caacaagctg aacaaaatcg
     1021 agtgattgcc aataaaattt tggttcagta ttacaaggag tttcaagaga gaaaccctaa
     1081 tttggctgtt tataatgctg ttattcataa tgacgaaata agtccgcacc tgcacttgaa
     1141 tattgtgccg gttgctgaag gttataaacg gggtgttcaa aagcagccga gctttaataa
     1201 agcactggtt caacaaggga tacacgctga taaagataat ggtcgagtgc ttttcaataa
     1261 ttttagaaat caagaagttg actctataga acgattaatg aatgattacg gttggaaaag
     1321 ggatattgtc ggtaccaaca aaattaaaga tattcacgaa tacaaagaaa tcatgagtga
     1381 aatttctgaa ttgcgccaaa cggctgtaaa agagcgtgag agcgtttctg atgagttgag
     1441 gtatatttat aggcaaagag aagaaaacga gcgtgataag gctctgatgg cttctgagag
     1501 agcgaagatt gaagaagaga agaaaaatat gctgaatgta aaaaaagaga tggcggtcat
     1561 tttccaaaaa gatatggttc ctttagaaag tttcaagagg cttcctgatg gttcgtatca
     1621 tttatctggg aatgattttg ttgatttgta tcaacgagct acgagagcaa caaatgccaa
     1681 gagaaatgct gataacatga gtgagcaata tttacagatg cttgagcaaa atacagaatt
     1741 aaatgagtat gtagctacat tggaagaaga gcgtcatttg tctggtagaa aacaattagc
     1801 agatttacaa gtggaaaata aaaagctgaa agaagaaaat cgctctttac gccagcgttt
     1861 agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt ttgtctatcc gttttggtct
     1921 tgcagaaaag gatatggggc tttctgagga attagaagta ttagagcagg agaaaacctt
     1981 attttatcgt caaaacgtct cacagcataa attagagcgt tctgagaatg agattgagtg
     2041 gtagccacga tgtgcagtca acggtaccgg aggcggtttg tgccgaggat aaggagttga
     2101 cggctaaacc actagcccct gaagctgaca tttgctaaaa aatggcaagt ccattttttg
     2161 gccaacttag cgttagcgaa gtttgggggc gcccttttca aagggtgcgg ttttattttt
     2221 ttggtatttg catagctaaa gagaagtgct attattgagt tagcaaacaa atgaactgga
     2281 aaaagagcaa aaaaataagt cgagcaacgc taccaacgtt ctcgaccttt acggaaacta
     2341 accacactag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagact
     2401 agagattgat ggttatgaat ttttagagtt acgtgaactc tggaaattct ttccaaaaat
     2461 ctcttttttc tttgaggagg aactgaatat gagtaaaaaa agaacaatcg tgaatgagtg
     2521 ggaaaaatta acagccctgt ctaacaggcg gttaaatgtt caaactttgg tacaaactat
     2581 aaaattaaat tggtcgattg accgaattac cattgttgga aagttgaatc aaaatatttt
     2641 ttatcatact caacatgatg tattaatatt agattttgaa cagcttatgc gggtaaatga
     2701 aggcaatggc tatttaaaag cggttggaaa taatggttgg cagctattag accaacatga
     2761 cgaaaatatt gcctatattg aaatttctaa atggcaagaa ggaaaagggc ggattgattt
     2821 taatcctaat aaaatcagtc gatttttagc ggattccatg aaaaatttta ttcatgattt
     2881 atttttagag ccccattttt cacgagcgga tgttgcctgc gatcttattg atgtgcctga
     2941 tgagtttatc acgcaatatc gaattattga tccagtttca tttaaattaa tttatggacg
     3001 ctcaggaaaa ctggaaacgg tgtattgggg tagtcgggca agcgagcggc aaattcgttt
     3061 gtacaataaa aaaatagagc aagaaatgaa aagaaaggtg gtgccttcag aaattaatac
     3121 ttggtggcgg ttagaaatgc agttaagacg tggtaaagct acggaatggt atgctatggt
     3181 gaaagaaagt ttggatagct ttgtgagtcc acatttttta ccaactgata taaaacctac
     3241 ggataaagtt atgattgatg gattgttagc taatcattct aattgggcga ttttgtcaag
     3301 aaactcgaaa tataaatatc gaaatttatt aaagcgagaa agtcaaaatg atgagttgac
     3361 gaatcattta cgagaaacat ttgaagaatc agcagatgat ttaaaaaaag aattggatac
     3421 ttggttacag ggacttgatg tgacagagga ggaatagtaa tggcagaaaa acaaaagcat
     3481 tttggtaaga aaaattggtt ggtgaattta acaggtgata acgttgtcgt tgtttttttg
     3541 gatagcaaaa aaatgtccgg taagttactt gatgtaacgc agtatgagtt gattttgaaa
     3601 aataataaaa ttgattcccc aatcattatc cctaagcatg ctgttaaata tgtttatggg
     3661 gtagctgaaa gtaacggaga agataatgac tgatttgcca gaaaaaatga ccataaaaaa
     3721 ccttgctgat gagttgggta tatctaaaaa tacagtgcgc aatcaactgg tcaatgcagg
     3781 ttatgacctt gctcggtata aagataatgg ggttatcatg ttagacaagg aattgattgg
     3841 tgttgtgcgg tcaatttatc ggtcaaaagg tggtcaattg actggtgatt taccggtcaa
     3901 tgatgcaggc aatgatttcc cgttagaaaa agaaaatgaa tttttgcgtg aacaattagc
     3961 ggaaaaagat aggcatattt ctgaattaac ggaaatgatt cgtgaccaac aaaaattgac
     4021 tttgcttcag caacaaaaaa acgatcagtt gttactagaa aatcaagaat taaaaaatcg
     4081 aaaatggtgg caattctgga agtgaggtgt gacatggata aaattgattt gagagaagaa
     4141 acagtcgata gaattgaatt gatgtatgaa cggttagctg attatttgaa tttgccggat
     4201 gaagataatt atagtgaaat gcttgaacga attatggatg ttctcaactc ggttatcaat
     4261 gctgatctag caaaatcttc agttttgact aatgaatttg ttcataagca tttagcgcaa
     4321 acaactgatg aatcagaaaa agaattttgg ttgaaattaa tgagtcatgt ta
//
