LOCUS       NZ_LR135494             5569 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8414 plasmid 7.
ACCESSION   NZ_LR135494
VERSION     NZ_LR135494.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885787
            Assembly: GCF_900639715.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:15:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,505
            CDSs (total)                      :: 3,414
            Genes (coding)                    :: 3,291
            CDSs (with protein)               :: 3,291
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 42 of 123
            Pseudo Genes (incomplete)         :: 78 of 123
            Pseudo Genes (internal stop)      :: 26 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135494.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8414"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            complement(638..793)
                     /locus_tag="EQB57_RS18465"
     CDS             complement(638..793)
                     /locus_tag="EQB57_RS18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846310.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRIF
                     EQRKKM"
                     /name="hypothetical protein"
     gene            complement(812..1381)
                     /locus_tag="EQB57_RS18175"
     CDS             complement(812..1381)
                     /locus_tag="EQB57_RS18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_002322960.1"
                     /translation="MTIKWIISIAYLVLTFAIPSLGVPSNIYFLFEHSDIFFLVLIIIL
                     FHSTFIREFKEVNLKKLFKYSFFSFSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAA
                     TYGAFLEEGIFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQ
                     TKNIWYSIVAHGFYNTIGILIYLISI"
                     /name="type II CAAX endopeptidase family protein"
     gene            complement(1381..1836)
                     /locus_tag="EQB57_RS18180"
     CDS             complement(1381..1836)
                     /locus_tag="EQB57_RS18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="WP_002322959.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDDE
                     YYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIARNT
                     SASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
                     /name="LMxysn_1693 family intestinal colonization protein"
     gene            complement(2456..2872)
                     /locus_tag="EQB57_RS18185"
     CDS             complement(2456..2872)
                     /locus_tag="EQB57_RS18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2874..3155)
                     /locus_tag="EQB57_RS18190"
     CDS             complement(2874..3155)
                     /locus_tag="EQB57_RS18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(3531..4055)
                     /locus_tag="EQB57_RS18195"
     CDS             complement(3531..4055)
                     /locus_tag="EQB57_RS18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_044383356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(4072..5040)
                     /locus_tag="EQB57_RS18200"
     CDS             complement(4072..5040)
                     /locus_tag="EQB57_RS18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     Typing          complement(4077..5040)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cttaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccctaaagtt gtctgaatgc
       61 cctctaaaat tcgatttaag cactcttaaa cctactctta tatatttata tacttagacg
      121 tttcaaacgt cttaaaacgc aaattagagc ttgataaaga tatttgctcg tgtgccgact
      181 tttttaaaag tctaggcaca cgccatgaca aagctgaaat aaactgcttc agctttgtca
      241 tggcggattc gctcacttga aaaagtgttc atttcgctcg attattagca tatatttttt
      301 aacttttttt gctagccaaa ttttgcatta gttcgctcat aaatcccacc ctttcagtac
      361 gtcctacgga cgtatcgttc gtaatgtgaa agtaccttta aaggtacttt ctacaaaact
      421 cactaaagtg tttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa
      481 taaccgcttg tcaaaggggc gcaagctccc ccttgaatcc cccctcaaaa cccacgctcc
      541 acaatacaaa aaaggctagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc
      601 ttcggtgaaa ttgttgcttt gcaacgatca ggtagagcta catttttttt cgttgttcaa
      661 atattcttac gccatttact attaaaatcg taggtattcc aaagtaaaga aaaaattgat
      721 tagcaggtaa tacaaaatat aaaataatac cagcaataat taccaaactt atagtaagtt
      781 ttgttaattt catgaagtat gccaccttat cttatataga tatcaaatag attaatatgc
      841 cgattgtatt ataaaatcca tgagcaacta tggaatacca tatatttttt gtttgtatat
      901 aaacaaaact taaaataata ccaataaaga aaatacttag gcctccccca tgaacaatag
      961 aaaacagaaa agaagaaatt agtattgaaa tatattgttg ttttttgttt gcttgaggat
     1021 caatcataca aaatcgaaaa attccttctt ctaaaaaagc tccataagta gcagcattta
     1081 aaaagaataa aaggagagaa ccatttactt cattaggatt tataccttct gaaaaagatg
     1141 tatttagaat attgattagg aataaaacag agaatgaaaa aaaactatat ttaaagagtt
     1201 tttttagatt tacttctttg aattcacgaa tgaaggtaga atgaaatagt ataattatta
     1261 gcactaaaaa aaatatatca ctatgttcaa ataaaaaata tatatttgac ggtactccta
     1321 atgatgggat agcaaaggtc aaaaccaagt aagcaatact aattatccat tttattgtca
     1381 ttaggcagga atataatcaa tatattgtgt atttgctgta gaagtttgag tacgaatata
     1441 tttattatct accttacgat aaactctgta ggttacagtt attcttgcat tttgacgata
     1501 ttttttaaat tttccacttg cagaagtgtt tctggctatt tcgattttca tcccaaatac
     1561 atttgcactg acagataact ttgtacctcg actatgagtt tctataccta aataagtacg
     1621 gcttccttta gcaattgacc agctttgagg tttagaccaa gtccttgttt gaataactgg
     1681 ttcgtctaac acatagtagt actcatcatc agaataagtg gctatatcag aaatgttgtt
     1741 ctcattagcg aataccgctc cgctaggagt aatagcagaa ccaaaagtta aaatagaagt
     1801 agataataaa aaggttttaa ataactgttt tttcatcaaa atcacctcga atataagata
     1861 cttaagaaaa cgctttcttt atataattag aataatcagg aaatttaatg ttgtcaagag
     1921 ggtttatttt ataaaagctg ttatattaaa ggttagtaga cataatgtcg actatgccaa
     1981 gtagcccctc tatcaagggc tatagctgct cactttgtga tttttaattg gtctagctag
     2041 ttactagctt ctaatgcttg attttacgcg atttctcttt tttagttctt tgtgcatttt
     2101 tatctgtcat tttttaaaga cttgttattt ttgagaggat ttgatatgct ttttttgtct
     2161 ttctctagtt cttcactttg taatattttt atgttgatga ataggataat ctcattttgg
     2221 gctgctcgtg agagcagtct ttttttgttt cagggtacgt cctattcttt ggatttccgc
     2281 gctattaaat aaccgatact tggggattgg ggtagcgccc caaaatacaa aaagaagaag
     2341 ttgcataagc ggatttttct tttttttcgc aagttggtac caacttgcaa atcttgctgg
     2401 aagactgatc cctaaaaaga aatcgaggaa tttttttagg gatcggtttc tttttttatc
     2461 tttttagaaa gtttgttggg tcaaaatttc cagaagtaaa ggtttgattg gtttcttttt
     2521 gacgattttt gggttgattt cgtcgggaag gattgagttg attttcaatt ttttctttca
     2581 cttcttgata cactttagaa tttgtaccga taagaacacc agtatctttt agatataaat
     2641 cagcaaattc tgccatgatt tctgtcatga tagaaatttc ttttcccatg gcattgagtt
     2701 ttgcttggtt actggttgtg gttgtattgg tatcaggttc ataactcata cataaaaaac
     2761 gaatggcttc tgaataattg ttgacgtctg gatttttttc gatgatggaa tctaaaacgt
     2821 caacgtgact gggataaagg gttacgtgtt tttgaacaga agtttttctc atgttatatt
     2881 cctccgataa gtgtttcaat gagttggttg tttgtgtgaa catagtcatt cagtatgttc
     2941 gtattttctg tttgaatggc aataaatttt tcgagtaata aatcgaattg atcttttttg
     3001 taattcataa gttgacggtc aaaatcactt tcgattagaa cttttagata tttatttcga
     3061 ctccattttt tgcccgtttt ggttgtgagt tcctgacaac gtttatctat tcttgcaata
     3121 attttgggat tgatatttcg aactaaaatg tccatggaaa ttttcctttc gtatcagcgt
     3181 ataatttatg tataccatac aaatatagta tttgtatagc tatttttcgc caaaacacta
     3241 tacatagcct agccacaatg gcaaaaaagt gatagcaaat ttttttctga gaaacgtgat
     3301 aggcacagcc tatctagcac ctttcgtaag aaagtgatac cacgtttcct tatgctcttt
     3361 caaaattcag aaaaatcagc tgtaaaacga gtgcagtccg agtaagattc aacgaaaaaa
     3421 cggggattgg ggcgccacca aaatatcgca acatgggtcc atgttgctct gctcgcaaaa
     3481 gcaaaaagaa gaataaaacc agcaattttt attaatttaa attgctggtt ttattgccag
     3541 aattgccacc attttttctc cgcctttgat tgtttttcta tcgattctgt ttcttcagaa
     3601 agagtcgttg atttattttc cgaagattct tccgtataag tcaactgtag ttgttcctgc
     3661 agtttctcga tttgttggtt ggcttgcagg gtgagttgtt gttggtggtc gagtaatttt
     3721 tgcatttgtt ttatttgctc gtctttttct tgtagttgca tatctttttg atttaactgt
     3781 tgttctagga gagaaaggac ttctaattcg ggaacttggt tggcgttttt gtggttttcg
     3841 gttttcgttt tttcgccaac tagttgttga tttgagtttt cgttttcgtt aaaaaacatt
     3901 gattttatag ccttttgtcc ggtttcatta atcacaaact tgtttccgat tttcgaaaac
     3961 caaagttttc gattttcgtc agaaactttt ttgttaattg ctgttttact aactcctaat
     4021 tcttcagcaa gttctttgat tgttttttct gtcataaaat ctaaaaaccc ctcaatcttt
     4081 gaataagttt tgaagattgt ctaataagtc tttttttagt tctgaaggaa tatcggaatt
     4141 tatctctgat ttttgtgcat tattttcttt attttgggta ttttgttctc tttctaactt
     4201 ttctaactgt tgtaaaagtt ccaattttcg cttttgttct ttgatttttt cttgtaatga
     4261 agtaggtgct ttttcagatt gaacttgttg gttagctagt tctaatgctt tgttttgttc
     4321 ttcttttgtt ggggcatgtt tgttaaagtc gtctgatttt tttatctcag gtttaaatgt
     4381 aaattgatag ccaatgacag gctttccccg tccttttccg tatttctttt tgattgttaa
     4441 acctttaaag attgcggtta gttcttgtcg tattggattg aacacacgtt gatcaatcgt
     4501 accttggttg taacttttag gaatatcaag taattcatga aagtcttctt ttgaaaaata
     4561 agcgaaacct ttggttctgt attgttttag taatcgaaac attgtttttg aataactgct
     4621 ttctaattca ttgaattgtt ggagagaata gcgcacccat tcttcaagat tattaagtaa
     4681 gggtaaggct ttttcatgaa cttgtatttc agcaaaagga atatcagatt ttccgtcgat
     4741 tttaaattga ttaaataaaa caaaacgttc acgttgcaaa ccgtcagcac ttcttctacc
     4801 aaaacgtaaa tccattaatt tgtcatacgt tgtttctaaa tcatctatga atcgaacatt
     4861 tgcagttgct ttataattgc ttaaatcttt taattgctcg aatgtaaatt gtactttttt
     4921 atcacctaca tcacgcattc ttgatacgat agaaaagaac aaattcattt cgactggtgt
     4981 aaattttcgc aacggaattg tatttaattc atgatgatac tttacgattt cattagacat
     5041 atttaaaata gctcctttct tactcgcaca agagatacac gttatgaaat acaaaatgcc
     5101 tgtttatcta catcttatat taacatcgtc gatgtactaa ttcaatgtac aaacagacaa
     5161 aacgatgtag ataaacagac aaaacgatgt agataaacag acaaaacgat gtagataaac
     5221 agacaaaacg atgtagataa aagcgtcatt cccttgggag agtaaggata tagccgacgg
     5281 taaagaggtt taaaaagagg tttaaaaaga gattataaaa gagggggaca aaatttcccg
     5341 cctaaaaaac cacttctttt ttctccaaac agattcgcgc atttttctca actgggaatc
     5401 ttactcccta cagtcgctta aaaaggtgtg atcgctctgc tctcacatta tactgcgcag
     5461 tcgcttccgg ctcctttgct aaaaatgttc ataaggaact ctttgagaag ctatgagcct
     5521 tcgtttgaga cgttttaaga cagtcttagt ataaatccct tatcttgtt
//
