LOCUS       NZ_LR135492             8584 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8414 plasmid 5.
ACCESSION   NZ_LR135492
VERSION     NZ_LR135492.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885787
            Assembly: GCF_900639715.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:15:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,505
            CDSs (total)                      :: 3,414
            Genes (coding)                    :: 3,291
            CDSs (with protein)               :: 3,291
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 42 of 123
            Pseudo Genes (incomplete)         :: 78 of 123
            Pseudo Genes (internal stop)      :: 26 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135492.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8414"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(123..611)
                     /locus_tag="EQB57_RS18060"
     CDS             complement(123..611)
                     /locus_tag="EQB57_RS18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(621..1589)
                     /locus_tag="EQB57_RS18065"
     CDS             complement(621..1589)
                     /locus_tag="EQB57_RS18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     gene            complement(<2277..2422)
                     /locus_tag="EQB57_RS18735"
                     /pseudo=""
     CDS             complement(<2277..2422)
                     /locus_tag="EQB57_RS18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(2882..3208)
                     /gene="bacA"
                     /locus_tag="EQB57_RS18080"
     CDS             complement(2882..3208)
                     /gene="bacA"
                     /locus_tag="EQB57_RS18080"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_181047671.1"
                     /translation="MEAASSRYNHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQI
                     ASFIPIGGVGTWVITNAFSSAVDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYAR
                     YVIK"
                     /name="bacA"
     gene            3462..3866
                     /gene="tnpA"
                     /locus_tag="EQB57_RS18085"
     CDS             3462..3866
                     /gene="tnpA"
                     /locus_tag="EQB57_RS18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            3883..5031
                     /gene="tnpB"
                     /locus_tag="EQB57_RS18090"
     CDS             3883..5031
                     /gene="tnpB"
                     /locus_tag="EQB57_RS18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(5810..6958)
                     /gene="tnpB"
                     /locus_tag="EQB57_RS18095"
     CDS             complement(5810..6958)
                     /gene="tnpB"
                     /locus_tag="EQB57_RS18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(6975..7379)
                     /gene="tnpA"
                     /locus_tag="EQB57_RS18100"
     CDS             complement(6975..7379)
                     /gene="tnpA"
                     /locus_tag="EQB57_RS18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            complement(7712..8128)
                     /locus_tag="EQB57_RS18105"
     CDS             complement(7712..8128)
                     /locus_tag="EQB57_RS18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(8130..8411)
                     /locus_tag="EQB57_RS18110"
     CDS             complement(8130..8411)
                     /locus_tag="EQB57_RS18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     Typing          complement(622..1589)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaaagtgat accgcgtttc cttatgctct ttaaaaaata gcgacgtgag atcatgtcgc
       61 tatgcttgta aaagcaaaaa aagaaagtaa cccaacaatt tttatgaata aaaattgttg
      121 ggttattgcc agaattgcca ccattttttc tccttgtttt gtcctttttg agtttcttca
      181 gtaacagaaa ggggttcttt ttctgaagat tcttctgcat aggttagctg gagttgttcg
      241 tgaagttttt tgatttgttg attggcttgt agggtgagtt gttgttgctg gtcgagtaat
      301 ttttgctgcg tttctaaagc tatttgaagt ttttcaattt gcctatcttt ttcttcaata
      361 tgattattct tagtttcaat ctccttgagt aggtaatcgg taggtaatga atcgggtaat
      421 tcactaggta agttacccga ttcattacct gataaaacct ttgtaattgt tgttactgct
      481 tcttttttca ggtaggtaat atttcctttt ttgtaggtaa aattactagg taatttcctc
      541 agttgatact tcactttatc ttttgaaaca cctagaatat ctgctaattc tttgatagtt
      601 ttatctgtca taaaaacccc ttattcttta aataagtttt ggagattatc tagtaagttt
      661 tctttaaatt ctggggtaat atcggaattg ttatctgcta tttgtttgtt aattttctta
      721 cttggggtat tttgttttct ttctaattct gctaactgtt gtaaaagttc taattttcgt
      781 ttttgttctt tgattttttc ttgtaatgaa gtaggttctt tttcagattg aacctgttga
      841 ttagctagtt ccaaagcttt attttgttct tctttggttg gagcatgttt gttaaagtcg
      901 tctgattttt ttatctcagg tttaaatgta aattgatagc caatgacagg ctttccccgt
      961 ccttttccgt atctcttttg gattgtcaaa cctttgaaga ttgcggttag ttcttggcga
     1021 ataggcttta taattttttt atttatatca ctttctctat aacttttagg gatatcaagt
     1081 aattcatgaa agtcttcttt tgaaaaattg gcaattcctt tggttctgta ttgttttaat
     1141 aatctaaaca tggttttgga ataactgctt tctaattcat tgaattgttg gagagaatat
     1201 cgaacccatt cttcaagatt attaagtaag ggtaaggctt tctcatggac ttgtatttca
     1261 gcaaaaggaa tatcagcttt tccatcgatt ttaaattgat taaataaaac aaatcgttca
     1321 cgttgcaaac cgtcagcact tcttctacca aaacgtaaat ccattaattt gtcatacgtt
     1381 gtttctaaat catctatgaa tcgaacattt gcagttgctt tataattgct taaatctttt
     1441 aattgttcga atgtaaattg tactttttta tcgcctacat cacgcattct agatattata
     1501 gagaagaata aattcatttc tactgacgta aatttgcgta aaggaatcgt atttaattca
     1561 tggtggtatt tcacaatttc atttgccata aaatcctcct tcaagattaa ctacttaaca
     1621 aaattataaa gagctttcta atgggggtca accccctaat taaagacccc taaaaacaga
     1681 caaaaagacc cctaaaaaca gacaaaaaga cccctaaaaa cagacaaaaa gacccctaaa
     1741 aacagacaaa aagaccccta aaagattctg cacaccttgg gagagtaagg atacagccga
     1801 cggtaaagag gtttaaaaag aggttataaa agagattata aaagaggggg gcaaaatttc
     1861 ccgcctaaaa ttttcctact ttttttctcc aaacaggttc gcgaaatttc ctaaactggg
     1921 aatctgctcc ctacggtcgc tttaaaggtg tgatcgctac gctctcacat taaactgcgc
     1981 agtcgcctaa aggctccttt gcttaaaaac agttcgtaag gagtcctttt agaagctctg
     2041 agcgctcatc tacggcgttt taagacaagt ctagataaaa tcccttatct tgtttttaaa
     2101 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aacgttctac agaacctcta
     2161 aaattcgttt taaggcgttt taaagtcgct ttggtataat tacataccag aacgtttaaa
     2221 acgccttaaa acgcaaatta gagccctatg tatatatcga ctaatgttac ctagactttt
     2281 acggaagccg gcacactcat ttgcaaagct tcagagttgc aaatttggat tcgctcactc
     2341 gaaaactgat ttattttgac tctgatcaat aggtgtattt tttagaagga ttgctagcca
     2401 aattttgctt taatttgctc ataagtccca cccttttgat acgtccatag gacgtatcgt
     2461 ttgtaatgtg aaagtgacta taaggtccct ttctacaaaa cacactaaag ggcttggcaa
     2521 gctctatatt tgctagccaa atacctccgt atttggtgca aataaccgct tgtcaaaggg
     2581 gcgcaagctc ccccttgaat ccccctcaaa acccacgctc cacaatacaa aaagtggcta
     2641 gaaaatgcta gccacttttt gtaaaattta gtatgattta tttttagcta tttagagttt
     2701 ttatttccgt aaatacttct aaaaattgct aagataaatg ctataggaat tagataaaga
     2761 atactttttt gtaagcttat attgaaaatt agcctaaata tgaagaacat agtgattaaa
     2821 actattattg aaataattac agctaaaatc tttttattca tgagatagtt tcactttctg
     2881 attattttat tacataacga gcgtaatcat tgtattgata tgatgttgta ttgctaaagt
     2941 gagcattata tgttaattgt acaccttttc cttgatttgc agctcttaca aagacattca
     3001 ttgcatcaac agcactacta aaagcatttg taataaccca tgttccaact ccaccaatag
     3061 gaataaaaga tgcaatttga gcaggagtct ttagcttatt ataatttttt ttcaaatctc
     3121 tacacatttt attagtaaca taatagttca ttttatagca agtaaatcca cgatgattat
     3181 gattatatcg tgaacttgca gcttccactt ttatacctct agcactaaaa ggaaacagat
     3241 ttaacggagt acttgctcca acaatcatac cgaacaaaaa tagacaagta ataattttaa
     3301 cagaattagg cagaagtcgc ccatcctcta taggtgggtg atgaatgccg ttcggtatga
     3361 gggttaccgt tggtaactcg aagaccgaca actaaggcgt attgcctttt tattttgttc
     3421 catgttacaa tcagtctata caagaaatga gttgatataa catggaatta gatacaaata
     3481 atcattcagt atttcttctt tattaccacc ttgttttagt aacgaaatat cgtagacaag
     3541 tgattgatga tgaaatatct gattatgcca aaactacttt tgaaagaatt tcagaatcgt
     3601 atcacattac tttagtggag tggaatcatg ataaagacca cgttcatatt atgttcaaag
     3661 ctcaacctaa aacagaattg acaaaattca tcaatgccta taaaagtgcc agctcaagat
     3721 tgataaaacg tgactttcca agagtcaaac aatttctttg gaaagaaatg ttctggtcta
     3781 aaagtttttg tcttctgaca actggtggcg caccaattga tgtgattaag aagtatatcc
     3841 aaaatcaagg aaacaatcat aaatagaaag taggtgaact ttatggaaca actaaaagca
     3901 tacaaattca gaatctatcc aacagaggaa caagaaatct tctttgccaa atcttttggt
     3961 tgtgtccgta aggtctacaa tctaatgctt gatgaccgaa aaaaggcgta tgaagaagtt
     4021 aaaaatgatt cttctaaaaa aatgactttt ccgacaccag ctaaatacaa gaaagaattt
     4081 ccgtttttga aagaaattga tagccttgct ttagccaatg cccaacttaa tttagataaa
     4141 gcatacaaga atttttttcg ggataaatcc gttggatttc cacgtttcaa aagtaagaaa
     4201 aatcctgttc agagttatac aacgaataat caaaatggta cagttgcttt gattgatagt
     4261 aaattcatca aagttcctaa attgaagtca ttagtaagaa ttaagcttca tagacaacca
     4321 aaagggatta tcaaatctgc tacaatatca cgtcattcta gcggtaaata ctatatttct
     4381 ttgttgtgta aagaagaaat tatcgaatta cctaaaacta attccacaat tggtattgac
     4441 ttaggtatta tggactttgc gattctttct gatggacaaa aaattgataa taataaattc
     4501 acgtccaaaa tggaaaagaa actaaagcgt gaacaacgca aattgtctag gcgtgcttta
     4561 ttggctaaaa ataaaggtac caatctattt gaagctaaaa attatcaaaa acaaaaacgc
     4621 aaagtagcta gattgcatga aaaagtaatg aatcaacgta ctgattttct gaataagttg
     4681 agtacagaaa tgatcaaaaa tcacgatatt atctgtattg aagacttaaa cacaaaaggt
     4741 atgttgcgaa atcataaatt agctaaaagt atttctgatg tttcttggtc aaagtttgtg
     4801 actaaattac aatacaaggc tgactggtat ggacgaaaaa tcatcaaagt agataaatgg
     4861 tttccgtcta gtcagatttg ttcagagtgc ggacataaag atggcaagaa atctcttgaa
     4921 attcgagaat ggacttgtcc tgtttgtcat actcatcatg atcgtgatat caatgctagt
     4981 atcaatattc tgaccgaagg tctaagacta cattctatgg gattggctta gatccaatca
     5041 tcaagaaccg taggaactac ggggatagct tggtaaataa gagacaccgc tgttagtaaa
     5101 gaaatacgct atcaagtatg ctctattccc aagaagctcc cacttcaagc gttaagaacc
     5161 gtcaggttta gctaagtggt gagtagttca ctaattttat ttttcattgt tacttcctcc
     5221 ttagatataa aatcctagga aagcgctttc tgtatatatt tagaataatc agaaaattaa
     5281 atattgtcaa gagggtttat tttataaaac gtgttatttt aaaggttagt tgacataatg
     5341 tcgagtatgc caagtagccc ttgtatcaac cgatacaggg gctcactttg tgatttttca
     5401 ttggtctagc taaaaggtac gttcggaagc ttgattttcc gctattgtct gtttacgatt
     5461 ctttgtgaat cttaaccgat tcctttttag aaacttgtta tttttcataa aatatggtaa
     5521 atttttggca gcgcttcata agcgcgctgc tttttcaaga ataaacgctc attatttttt
     5581 attggctgct cgttagagca gtcttttttt atttcagagt acgttctatt tttttgagtt
     5641 accacgctag tgaactactc accacttagc taaacctgac ggttcttaac gcttgaagtg
     5701 ggagcttctt gggaatagag catacttgat agcgtatttc tttactaaca gcggtgtctc
     5761 ttatttacca agctatcccc gtagttccta cggttcttga tgattggatc taagccaatc
     5821 ccatagaatg tagtcttaga ccttcggtca gaatattgat actagcattg atatcacgat
     5881 catgatgagt atgacaaaca ggacaagtcc attctcgaat ttcaagagat ttcttgccat
     5941 ctttatgtcc gcactctgaa caaatctgac tagacggaaa ccatttatct actttgatga
     6001 tttttcgtcc ataccagtca gccttgtatt gtaatttagt cacaaacttt gaccaagaaa
     6061 catcagaaat acttttagct aatttatgat ttcgcaacat accttttgtg tttaagtctt
     6121 caatacagat aatatcgtga tttttgatca tttctgtact caacttattc agaaaatcag
     6181 tacgttgatt cattactttt tcatgcaatc tagctacttt gcgtttttgt ttttgataat
     6241 ttttagcttc aaatagattg gtacctttat ttttagccaa taaagcacgc ctagacaatt
     6301 tgcgttgttc acgctttagt ttcttttcca ttttggacgt gaatttatta ttatcaattt
     6361 tttgtccatc agaaagaatc gcaaagtcca taatacctaa gtcaatacca attgtggaat
     6421 tagttttagg taattcgata atttcttctt tacacaacaa agaaatatag tatttaccgc
     6481 tagaatgacg tgatattgta gcagatttga taatcccttt tggttgtcta tgaagcttaa
     6541 ttcttactaa tgacttcaat ttaggaactt tgatgaattt actatcaatc aaagcaactg
     6601 taccattttg attattcgtt gtataactct gaacaggatt tttcttactt ttgaaacgtg
     6661 gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc tttatctaaa ttaagttggg
     6721 cattggctaa agcaaggcta tcaatttctt tcaaaaacgg aaattctttc ttgtatttag
     6781 ctggtgtcgg aaaagtcatt tttttagaag aatcattttt aacttcttca tacgcctttt
     6841 ttcggtcatc aagcattaga ttgtagacct tacggacaca accaaaagat ttggcaaaga
     6901 agatttcttg ttcctctgtt ggatagattc tgaatttgta tgcttttagt tgttccataa
     6961 agttcaccta ctttctattt atgattgttt ccttgatttt ggatatactt cttaatcaca
     7021 tcaattggtg cgccaccagt tgtcagaaga caaaaacttt tagaccagaa catttctttc
     7081 caaagaaatt gtttgactct tggaaagtca cgttttatca atcttgagct ggcactttta
     7141 taggcattga tgaattttgt caattctgtt ttaggttgag ctttgaacat aatatgaacg
     7201 tggtctttat catgattcca ctccactaaa gtaatgtgat acgattctga aattctttca
     7261 aaagtagttt tggcataatc agatatttca tcatcaatca cttgtctacg atatttcgtt
     7321 actaaaacaa ggtggtaata aagaagaaat actgaatgat tatttgtatc taattccatg
     7381 ttatatcaac tcatttcttg tatagactga ttgtaacatg gaacaaaata aaaaggcaat
     7441 acgccttagt tgtcggtctt cgagttacca acggtaaccc tcataccgaa cggcattcat
     7501 cacccaccta tagaggatgg gcgacttctg cctaattctg ttaaataacc gatatttgga
     7561 gattggggca gcgccccaaa atgcaaaaag aaggattcgc acaagcggat ttttcttttt
     7621 ttgtgcaagt taccggtaac ttgcaaatct tgctggaaga ctgcccccta aaaaagaaac
     7681 tgagaaatag aaaaaatttt cggtttcttt ttcatctttt tagaaagttt gttgggtcaa
     7741 aatttctaga agtgaatgtt tgattggttt ctttttgacg atttttgggt tgatttcgtc
     7801 gggagggatt gagttgattt tcaatttttt ctttcacttc ttgatatact ttagaatttg
     7861 taccgataag aacaccagta tcttttagat ataaatcagc aaattctgca atgatttcgg
     7921 acataataga aatttctttt cccatcgcat ttagttttgc ttggttgctg gtagttgtcg
     7981 tattagtatt aggttcataa ctcatacata gaaaacgaat ggcttccgag tagttattga
     8041 tatctggatt tttttcgatg atggaatcta aaacgtcaat gtgactggga taaagggtaa
     8101 cgtgtttttg aacagaagtt tttctcatgt tatattcctc cgataagtgt ttcaatgagt
     8161 tggttgtttg tatgaacata gtcgttcaat gtttcagtat tttttgcttg tatatcgata
     8221 aatttttcga gtaataaatc gaattgatct tttttgtaat gcctaagtgc ctggtcaaaa
     8281 tcgttttcga ttagaacttt taggtacgca ttacggctcc attttttgcc agtttttgtt
     8341 gtgagttctt ggcaacgtct atctatttct gaaataactt ttggatcaat attccgaact
     8401 aaaatgtcca tagaagaatt cctttcgcat ttgtgtataa tttatgtata tcatacaaat
     8461 atagtatttg tatagctgtt ttttgccgaa acactataca taagctatcc acaatgacga
     8521 aaaagtgata gcaaattttt ttcttagaaa cgtggtaggc acagcctacc tagcaccttt
     8581 cgta
//
