LOCUS       NZ_LR135486             3199 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4456 plasmid 5.
ACCESSION   NZ_LR135486
VERSION     NZ_LR135486.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884734
            Assembly: GCF_900639745.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,824
            Genes (coding)                    :: 2,690
            CDSs (with protein)               :: 2,690
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 54 of 134
            Pseudo Genes (incomplete)         :: 81 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 21 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135486.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4456"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            734..820
                     /locus_tag="EQB55_RS15295"
     CDS             734..820
                     /locus_tag="EQB55_RS15295"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_125669474.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1122..1346)
                     /locus_tag="EQB55_RS15300"
     CDS             complement(1122..1346)
                     /locus_tag="EQB55_RS15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1712..1996
                     /locus_tag="EQB55_RS15305"
     CDS             1712..1996
                     /locus_tag="EQB55_RS15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1999..2415
                     /locus_tag="EQB55_RS15310"
     CDS             1999..2415
                     /locus_tag="EQB55_RS15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2552..>3199)
                     /locus_tag="EQB55_RS15315"
     CDS             complement(2552..>3199)
                     /locus_tag="EQB55_RS15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_232043230.1"
                     /translation="AKQMDLFFALCARMKDRGLKKVRFSFEELKELSDYKMTATKAFVG
                     DLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPDLEYIINGLTAEF
                     SRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYYQMGNIDQKVL
                     NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     Typing          complement(2553..3199)
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attgaaattt ttcagcggta ctaaattcaa ttcattttga tatttgactg ttaattccgt
       61 tttttcatcc attcctttct tcccccctta caaataaata ataaccttta acaaccaact
      121 cgtaaatagg ttggttgtta ttatacaaaa ggatattagt actcagataa aaagttgtta
      181 gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc agataaaaag
      241 ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc taaaaagggt
      301 tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa aatttcccgc
      361 caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa ctgccaatct
      421 agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt tacttgcagc
      481 agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg tggcgtcctg
      541 agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct tcgttctaaa
      601 gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa aacttaggct
      661 tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt gcgactggaa
      721 ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc ttttaagcgc
      781 tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta aggcgactca
      841 gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg tttcgtgttc
      901 acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata tcctacaaaa
      961 gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg ctctgagagc
     1021 tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt ggtccaagct
     1081 aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga
     1141 aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc
     1201 attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt
     1261 tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga
     1321 aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa
     1381 ataaaatcac ttctttgccg tctatactct caaaaaaaat gtcgtttgaa aaactaaata
     1441 aatttttgat cgtgcatttt taaatttggt tatcgattac acgtgcagca ctcccccccg
     1501 aattttaaag agcataagga aacgtgtacc cactttctaa aaaaagtggg tagataggct
     1561 gtgcctatca cgtttctcag aaaaaaattt gatatcactt tttcgccatc gtgggtagct
     1621 tatgtatagc attttggcaa aaaacagcta tacaaatata gtatttgtat ggtatacata
     1681 aattatacat aattgcaaaa ggagattttt tatggacatt ttagttcgga atattgatcc
     1741 aaagtttgtt gcaacaatag acaaacgttg ccaagaactc acgaataaaa caggagaaaa
     1801 atggagtcga aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac
     1861 gacttacaaa aaagaccaat tcgatttatt gctcgataaa tttattgcta tccaaacaga
     1921 aaatactcaa atattaagcg agtatgtcta cacaaataat caaatcattg aaatgcttat
     1981 cggaggaatg atataaccat gagaaaaact tctgtccaaa aacatgttac tttgtaccct
     2041 agtcatgttg acgttttaga ttccatcatc gaaaaaaatc cagatatcaa taactactcg
     2101 gaagccattc gctttttatg tatgagttat gaacccgata ccaacacgac cactaccagc
     2161 aatcaggcaa aactaaatgc tatgggaaaa gaaatttcca tcatgactga aatcatcgca
     2221 gaatttgctg atttatatct aaaagataca ggcgttctta ttggcacaaa ttctaaagta
     2281 tatcaagaag tgaaagaaaa aatcgaagct aaactaaatc catcaaatcg aaatcgacca
     2341 aagaatcatc aaaaagaaac cagtcaaaca gtcacttcta gaaatttcga cccaacaaat
     2401 tttctaaaac gataaaaaac agataggagt tattcctatc tgttttttat ttagaagccc
     2461 catacaatat gctagaatgt actagcaatc ttagtcggtt gtaagggact tgtctacttg
     2521 cttaatgtaa ggggggcgag ttctttttta tttattcttc taaccagtta cgcagagaaa
     2581 tctttggcaa atctgacgtt aggcttgaaa aagtaaattc taatttggcg attttattgc
     2641 ccttttttgc ttttattttt gtgactttaa gatcattgaa gtattttttt agttctcgta
     2701 atgcaggatt taatactttt tgatcaatgt tacccatttg atagtattct gggatatcca
     2761 ttaagctcct aaactcttca atacgaacta cataataacc agtagaacgg tactgcataa
     2821 gcagtcggaa tagagttttt gcatacgtag aacgcaacga cgtgaaagca gataattcaa
     2881 atctactaaa ttcagctgtt aaaccattaa taatatattc taggtccgga ttaacactga
     2941 cttcaacaaa cttttttgtt ttattgattt caaaaccagt aaacaaaaca aagtagcgaa
     3001 tagaatcctc atcttcagta cggtaagtaa gattaagcat atctttatag agcttttcta
     3061 aatcaccgac aaaggctttt gtagcagtca tcttataatc acttaactct tttaattcct
     3121 cgaaagaaaa tctaactttt ttcaaaccac gatctttcat acgagcgcat aatgcaaaaa
     3181 ataaatccat ctgcttagc
//
