LOCUS       NZ_LR135452             4372 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Enterococcus faecium isolate E7114 plasmid 10.
ACCESSION   NZ_LR135452
VERSION     NZ_LR135452.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884989
            Assembly: GCF_900639665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900639665.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,307
            CDSs (total)                      :: 3,214
            Genes (coding)                    :: 3,062
            CDSs (with protein)               :: 3,062
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 61 of 152
            Pseudo Genes (incomplete)         :: 92 of 152
            Pseudo Genes (internal stop)      :: 32 of 152
            Pseudo Genes (multiple problems)  :: 27 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135452.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7114"
                     /db_xref="taxon:1352"
                     /plasmid="10"
     gene            complement(<1..1073)
                     /locus_tag="FSA55_RS16405"
     CDS             complement(<1..1073)
                     /locus_tag="FSA55_RS16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_284167072.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQ"
                     /name="plasmid recombination protein"
     gene            complement(1295..1657)
                     /locus_tag="FSA55_RS16410"
     CDS             complement(1295..1657)
                     /locus_tag="FSA55_RS16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045135959.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(1661..1969)
                     /locus_tag="FSA55_RS16415"
     CDS             complement(1661..1969)
                     /locus_tag="FSA55_RS16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333831.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(1978..2397)
                     /locus_tag="FSA55_RS16420"
     CDS             complement(1978..2397)
                     /locus_tag="FSA55_RS16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(2390..2623)
                     /locus_tag="FSA55_RS16425"
     CDS             complement(2390..2623)
                     /locus_tag="FSA55_RS16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(2626..3594)
                     /locus_tag="FSA55_RS16430"
     CDS             complement(2626..3594)
                     /locus_tag="FSA55_RS16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002347008.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(4039..>4372)
                     /locus_tag="FSA55_RS16435"
                     /pseudo=""
     CDS             complement(4039..>4372)
                     /locus_tag="FSA55_RS16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /name="recombinase"
     Typing          complement(2634..3503)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.164"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(7..1166)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.214"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tattgctcac tcatgttatc agcatttctc ttggcatttg ttgctctcgt agctcgttga
       61 tacaaatcaa caaaatcatt cccagataaa tgatacgaac catcaggaag cctcttgaaa
      121 ctttctaaag gaaccatatc tttttggaaa atgaccgcca tctctttttt tacattcagc
      181 atatttttct tctcttcttc aatcttcgct ctctcagaag ccatcagagc cttatcacgc
      241 tcgttttctt ctctttgcct ataaatatac ctcaactcat cagaaacgct ctcacgctct
      301 tttacagccg tttggcgcaa ttcagaaatt tcactcatga tttctttgta ttcgtgaata
      361 tctttaattt tgttggtacc gacaatatcc cttttccaac cgtaatcatt cattaatcgt
      421 tctatagagt caacttcttg atttctaaaa ttattgaaaa gcactcgacc attatcttta
      481 tcagcgtgta tcccttgttg aaccagtgct ttattaaagc tcggctgctt ttgaacaccc
      541 cgtttataac cttcagcaac cggcacaata ttcaagtgca ggtgcggact tatttcgtca
      601 ttatgaataa cagcattata aacagccaaa ttagggtttc tctcttgaaa ctccttgtaa
      661 tactgaacca aaattttatt ggcaatcact cgattttgtt cagcttgttg tttcgatatg
      721 ccagtagaag aaccatctgc tcgatttgtc tggaaatcat ctaaaatgcc tatttgcaca
      781 ataaattctc gttgaggttc caacttttta tccttgcgca ctttcgctaa atagtcctcg
      841 atttttctat cggctctttt ttgtttcgca ttatattttt ctaccacttc gccaaacaat
      901 tcctcatatt tatctgctag attcgtctgc tctaaataaa tattttcatc cgatttcgtg
      961 aagtcaatgt gcttatgcca atcattggtt ttctggcgat cagttaactc acgattatta
     1021 tgagaaatac ttgtattctt cgttcctttt ttaaatgaaa tactaaaccc caagttaaaa
     1081 ccctccttcg cgctgtcgct aataaaaaga ttgtaaacct ttctgttact ctttgtcaaa
     1141 gagtaacatt ttcctaattg tgacatggat gtcacaattc caaacgctct ccgagggttt
     1201 tcgctgtcac aagtgacaac gaaaaaagga gccgaaagcg tatcacgcta tcaactcctt
     1261 ttatgcttta ttttgcttca cgcaaaataa tcgcttatcg taaattcacg tcattaaata
     1321 ttgacgtaaa tttactctca ctcatctgac ttttcttatt cggtatcact ccgaataagt
     1381 cgtcagattc atcgggataa tctcgttcgc ttggcgctga tttttcatca gcaagccact
     1441 cattatccac tgttttttcg tgttgctgat cgtcagaaat tgacgataac caccgctcaa
     1501 aatcagcaaa agtttcaaac ccaaaacgct tgcgaagagc cttgcccatt gcagcatttt
     1561 ctttctcaat caagcgtttt ttctgttggc gaagtttcgc ttgttctttt tttaattttt
     1621 cttcttgcgc tttaatatct gctaaagtca ttcccataaa ctacatctcc ttttctttgg
     1681 aattaataac ggaaatttgg tttaactgca taacatgact cattaatttc aaccaaaatt
     1741 ctttttctga ttcatcagtt gtttgcgcta aatgcttatg aacaaattca ttagtcaaaa
     1801 ctgaagattt tgctagatca gcattgataa ccgagttgag aacatccata attcgttcaa
     1861 gcatttcact ataattatct tcatccggca aattcaaata atcagctaac cgttcataca
     1921 tcaattcaat tctatcgact gtttcttctc tcaaatcaat tttatccatg tcacacctca
     1981 cttccagaat tgccaccatt ttcgattttt taattcttga ttttctagta acaactgatc
     2041 gtttttttgt tgctgaagca aagtcaattt ttgttggtca cgaatcattt ccgttaattc
     2101 agaaatatgc ctatcttttt ccgctaattg ttcacgcaaa aattcatttt ctttttctaa
     2161 cgggaaatca ttgcctgcat cattgaccgg taaatcacca gtcaattgac caccttttga
     2221 ccgataaatt gaccgcacaa caccaatcaa ttccttgtct aacatgataa ccccattatc
     2281 tttataccga gcaaggtcat aacctgcatt gaccagttga ttgcgcactg tatttttaga
     2341 tatacccaac tcatcagcaa ggttttttat ggtcattttt tctggcaaat cagtcattat
     2401 cttctccgtt actttcagct accccataaa catatttaac agcatgctta gggataatga
     2461 ttggggaatc aattttatta tttttcaaaa tcaactcata ctgcgttaca tcaagtaact
     2521 taccggacat ttttttgcta tccaaaaaaa caacgacaac gttatcacct gttaaattca
     2581 ccaaccaatt tttcttacca aaatgctttt gtttttctgc cattactatt cctcctctgt
     2641 cacatcaagt ccctgtaacc aagtatccaa ttcttttttt aaatcatctg ctgattcttc
     2701 aaatgtttct cgtaaatgat tcgtcaactc atcattttga ctttctcgct ttaataaatt
     2761 tcgatattta tatttcgagt ttcttgacaa aatcgcccaa ttagaatgat tagctaacaa
     2821 tccatcaatc ataactttat ccgtaggttt tatatcagtt ggtaaaaaat gtggactcac
     2881 aaagctatcc aaactttctt tcaccatagc ataccattcc gtagctttac cacgtcttaa
     2941 ctgcatttct aaccgccacc aagtattaat ttctgaaggc accacctttc ttttcatttc
     3001 ttgctctatt tttttattgt acaaacgaat ttgccgctcg cttgcccgac taccccaata
     3061 caccgtttcc agttttcctg agcgtccata aattaattta aatgaaactg gatcaataat
     3121 tcgatattgc gtgataaact catcaggcac atcaataaga tcgcaggcaa catccgctcg
     3181 tgaaaaatgg ggctctaaaa ataaatcatg aataaaattt ttcatggaat ccgctaaaaa
     3241 tcgactgatt ttattaggat taaaatcaat ccgccctttt ccttcttgcc atttagaaat
     3301 ttcaatatag gcaatatttt cgtcatgttg gtctaatagc tgccaaccat tatttccaac
     3361 cgcttttaaa tagccattgc cttcatttac ccgcataagc tgttcaaaat ctaatattaa
     3421 tacatcatgt tgagtatgat aaaaaatatt ttgattcaac tttccaacaa tggtaattcg
     3481 gtcaatcgac caatttaatt ttatagtttg taccaaagtt tgaacattta accgcctgtt
     3541 agacagggct gttaattttt cccactcatt cacgattgtt ctttttttac tcatattcag
     3601 ttcctcctca aagaaaaaag agatttttgg aaagaatttc cagagttcac gtaactctaa
     3661 aaattcataa ccatcaatct ctagtcttga agaaacctaa aaatattcgt ataatgaatt
     3721 tgagttacgg aactagtgtg gttagtttcc gtaaaggtcg agaacgttgg tagcgttgct
     3781 cgacttattt ttttgctctt tttccagttc atttgtttgc taactcaata atagcacttc
     3841 tctttagcta tgcaaatacc aaaaaaataa aaccgcaccc tttgaaaagg gcgcccccaa
     3901 acttcgctaa cgctaagttg gccaaaaaat ggacttgcca ttttttagca aatgtcagct
     3961 tcaggggcta gtggtttagc cgtcaactcc ttatcctcgg cacaaaccgc ctccggtacc
     4021 gttgactgca catcgtggct accactcaat ctcattctca gaacgctcta atttatgctg
     4081 tgagacgttt tgacgataaa ataaggtttt ctcctgctct aatacttcta attcctcaga
     4141 aagccccata tccttttctg caagaccaaa acggatagac aaacgagtga tcgatttttg
     4201 aaatgcgttt tttaatcgct ctaaacgctg gcgtaaagag cgattttctt ctttcagctt
     4261 tttattttcc acttgtaaat ctgctaattg ttttctacca gacaaatgac gctcttcttc
     4321 caatgtagct acatactcat ttaattctgt attttgctca agcatctgta aa
//
