LOCUS       NZ_LR135451             5781 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Enterococcus faecium isolate E7114 plasmid 9.
ACCESSION   NZ_LR135451
VERSION     NZ_LR135451.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884989
            Assembly: GCF_900639665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900639665.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,307
            CDSs (total)                      :: 3,214
            Genes (coding)                    :: 3,062
            CDSs (with protein)               :: 3,062
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 61 of 152
            Pseudo Genes (incomplete)         :: 92 of 152
            Pseudo Genes (internal stop)      :: 32 of 152
            Pseudo Genes (multiple problems)  :: 27 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135451.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7114"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            complement(<408..553)
                     /locus_tag="FSA55_RS16370"
                     /pseudo=""
     CDS             complement(<408..553)
                     /locus_tag="FSA55_RS16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(854..1297)
                     /locus_tag="FSA55_RS16375"
     CDS             complement(854..1297)
                     /locus_tag="FSA55_RS16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(1270..2424)
                     /locus_tag="FSA55_RS16380"
     CDS             complement(1270..2424)
                     /locus_tag="FSA55_RS16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            complement(3040..3456)
                     /locus_tag="FSA55_RS16385"
     CDS             complement(3040..3456)
                     /locus_tag="FSA55_RS16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(3458..3739)
                     /locus_tag="FSA55_RS16390"
     CDS             complement(3458..3739)
                     /locus_tag="FSA55_RS16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(4035..4523)
                     /locus_tag="FSA55_RS16395"
     CDS             complement(4035..4523)
                     /locus_tag="FSA55_RS16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(4533..5501)
                     /locus_tag="FSA55_RS16400"
     CDS             complement(4533..5501)
                     /locus_tag="FSA55_RS16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     Typing          complement(4534..5501)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attttcctac tttttttctc caaacaggtt cgcgaaattt cctaaactgg gaatctgctc
       61 cctacggtcg ctttaaaggt gtgatcgcta cgctctcaca ttaaactgcg cagtcgccta
      121 aaggctcctt tgcttaaaaa cagttcgtaa ggagtccttt tagaagctct gagcgctcat
      181 ctacggcgtt ttaagacaag tctagataaa atcccttatc ttgtttttaa agcgccttac
      241 aggcggtcag ggcaagccct aacaacccta aaacgttcta cagaacctct aaaattcgtt
      301 ttaaggcgtt ttaaagtcgc tttggtataa ttacatacca gaacgtttaa aacgccttaa
      361 aacgcaaatt agagccctat gtatatatcg actaatgtta cctagacttt tacggaagcc
      421 ggcacactca tttgcaaagc ttcagagttg caaatttgga ttcgctcact cgaaaactga
      481 tttattttga ctctgatcaa taggtgtatt ttttagaagg attgctagcc aaattttgct
      541 ttaatttgct cataagtccc acccttttga tacgtccata ggacgtatcg tttgtaatgt
      601 gaaagtgact ataaggtccc tttctacaaa acacactaaa gggcttggca agctctatat
      661 ttgctagcca aatacctacg tatttggtgc aaataatcgc ttgtcaaagg ggcgcaagct
      721 cccccttgaa tccccctcaa aacccacgct ccacaataca aaaaaggcta gcaaatgcta
      781 gccacttttt gtaaagtttc gcttgtttca ccttcggtga aattattgtt ttgtaatatc
      841 aaacaactat tcatcaatca gctttaaaaa attcaattgg tttagctgtc ccttctgtcc
      901 catcattcgt tgtcaaaata aatacgagct tttttggaga atccaaacta aaagtgtaat
      961 ctaattgttt tttattagag cgcagtgtta gtaaattatt tgttgctttg taaatgaaaa
     1021 cttgttcatt tatatttaaa tttttatcaa tgatttgaat tttatgattg ttctctttta
     1081 ctaagatggt atattgttct atattttttg aagtatttaa attagtgatt actagacgtt
     1141 ctgaatgatc tttagtttgc caagttccta gaaaaggaat cagatcagtc ttttgtattt
     1201 cttgtttttt tgaacaacta gttaagaaaa gaactaacac tagacccata actaaagcaa
     1261 tgggtttatt taccaatttg taatggtatt gtcccaagta aaaccacctc caggtacagg
     1321 aattgtaact ggtcctgcat ttgcacttat tacagttggg tagttatacc aaggattcca
     1381 cacatagtaa gtttgataac caccttgatt gacccaccca aataatgcaa gtgcatgacg
     1441 gctgttacta taatttccat aaccagcagt tcccatataa atggcatttc cttttcttag
     1501 ttgtgtttgg acctctgaaa ggtttagtat tcgtgctact tttttaggat aaacaccaca
     1561 gtaatttgca tattttatta tttgatcttg attaagactt tttgattcaa tttgactgtt
     1621 gtttaaatta ggataggtat aatgcataat ggctctagct gaagtagcac gtacatctgt
     1681 tttgttacat aagatagcag cttctacgta agcagcacac cacggaaaac ttgtttgggt
     1741 ttctgctata cgccaattaa tcataataaa gttatctccg gaggagacac tttttacacg
     1801 tttaaccgca ggaaaatttt gttttttaga gaaattttta attttatttt ttatatttac
     1861 ggatataaga tcattttttt ctgtaaagga agaaacacca tttttttctg tacttaaagg
     1921 tgatttccaa agtagtttaa tttgtccatt atcggaataa taaatgctat tatcttgaaa
     1981 aaataaggtt gtatctgtta tattactatc tactagattt tgaagttctt ttgctaaaaa
     2041 ttgtgaaata cttcctgaat attctccatt tgtatttttt ttgacggtat aaatatattt
     2101 aatcgtatta tcagcgtctg aaaatactgg aaagaaaata gaactatttg ccgtattttc
     2161 tccatcgagc attttaaatg gcgaaccaag agtatagtta atatcagttt cggtgttatt
     2221 ttccattaaa agaattgaag ttatggagtg ttcaaaatca gttttcgcaa aattaattgc
     2281 ttgttcttca attggttggg agttgatatt gaaattagaa ttttctgaag cataggtatt
     2341 tgtaattcct atactaaaaa ttccaatcat caaggaaaag aggagaccta gataaatttt
     2401 ctttttgggt ttcaaaataa tcacgccttt gtgttaaatt taagtggtat acacctataa
     2461 ttaaaatata caatatgcga taacaaattt ttgttatagt tcataaaata ttgttttggg
     2521 aacataatcg ttatttatag cgttagttaa cataatgtcg agtatgccaa gtagcccttg
     2581 tatcaaccga tacaggggct cactttgtga tttttaattg gtctaggtaa aaagctcttt
     2641 ttaaggcttg attttctgca gttgcttctt tccgtttctt tgtgaatttt taccagtcat
     2701 tttttactgg gttgttattt ttcataaaat atggtaaatt tttggcagcg ctttataagc
     2761 gcgttgcttt ttcaagaata aactctcatt atttttttaa ctggctgctc gttagagcag
     2821 tcttttttta tttcagagta cgttctattt ttttgagtta ccacgctatt aaataaccga
     2881 tatttggaga ttggggcagc gccccaaaat gcaaaaagaa ggattcgcac aagcggattt
     2941 ttcttttttt gtgcaagtta ccggtaactt gcaaatcttg ctggaagact gccccctaaa
     3001 aaagaaactg agaaatagaa aaaattttcg gtttcttttt catcttttta gaaagtttgt
     3061 tgggtcaaaa tttctagaag tgaatgtttg attggtttct ttttgacgat ttttgggttg
     3121 atttcgtcgg gagggattga gttgattttc aattttttct ttcacttctt gatatacttt
     3181 agaatttgta ccgataagaa caccagtatc ttttagatat aaatcagcaa attctgcaat
     3241 gatttcggac ataatagaaa tttcttttcc catcgcattt agttttgctt ggttgctggt
     3301 agttgtcgta ttagtattag gttcataact catacataga aaacgaatgg cttccgagta
     3361 gttattgata tctggatttt tttcgatgat ggaatctaaa acgtcaatgt gactgggata
     3421 aagggtaacg tgtttttgaa cagaagtttt tctcatgtta tattcctccg ataagtgttt
     3481 caatgagttg gttgtttgta tgaacatagt cgttcaatgt ttcagtattt tttgcttgta
     3541 tatcgataaa tttttcgagt aataaatcga attgatcttt tttgtaatgc ctaagtgcct
     3601 ggtcaaaatc gttttcgatt agaactttta ggtacgcatt acggctccat tttttgccag
     3661 tttttgttgt gagttcttgg caacgtctat ctatttctga aataactttt ggatcaatat
     3721 tccgaactaa aatgtccata gaagaattcc tttcgcattt gtgtataatt tatgtatatc
     3781 atacaaatat agtatttgta tagctgtttt ttgccgaaac actatacata agctatccac
     3841 aatgacgaaa aagtgatagc aaattttttt cttagaaacg tggtaggcac agcctaccta
     3901 gcacctttcg taagaaagtg ataccgcgtt tccttatgct ctttaaaaaa tagcgacgtg
     3961 agatcatgtc gctatgcttg taaaagcaaa aaaagaaagt aacccaacaa tttttattca
     4021 taaaaattgt tgggttattg ccagaattgc caccattttt tctccttgtt ttgtcctttt
     4081 tgagtttctt cagtaacaga aaggggttct ttttctgaag attcttctgc ataggttagc
     4141 tggagttgtt cgtgaagttt tttgatttgt tgattggctt gtagggtgag ttgttgttgc
     4201 tggtcgagta atttttgctg cgtttctaaa gctatttgaa gtttttcaat ttgcctatct
     4261 ttttcttcaa tatgattatt cttagtttca atctccttga gtaggtaatc ggtaggtaat
     4321 gaatcgggta attcactagg taagttaccc gattcattac ctgataaaac ctttgtaatt
     4381 gttgttactg cttctttttt caggtaggta atatttcctt ttttgtaggt aaaattacta
     4441 ggtaatttcc tcagttgata cttcacttta tcttttgaaa cacctagaat atctgctaat
     4501 tctttgatag ttttatctgt cataaaaacc ccttattctt taaataagtt ttggagatta
     4561 tctagtaagt tttctttaaa ttctggggta atatcggaat tgttatctgc tatttgtttg
     4621 ttaattttct tacttggggt attttgtttt ctttctaatt ctgctaactg ttgtaaaagt
     4681 tctaattttc gtttttgttc tttgattttt tcttgtaatg aagtaggttc tttttcagat
     4741 tgaacctgtt gattagctag ttccaaagct ttattttgtt cttctttggt tggagcatgt
     4801 ttgttaaagt cgtctgattt ttttatctca ggtttaaatg taaattgata gccaatgaca
     4861 ggctttcccc gtccttttcc gtatctcttt tggattgtca aacctttgaa gattgcggtt
     4921 agttcttggc gaataggctt tataattttt ttatttatat cactttctct ataactttta
     4981 gggatatcaa gtaattcatg aaagtcttct tttgaaaaat tggcaattcc tttggttctg
     5041 tattgtttta ataatctaaa catggttttg gaataactgc tttctaattc attgaattgt
     5101 tggagagaat atcgaaccca ttcttcaaga ttattaagta agggtaaggc tttctcatgg
     5161 acttgtattt cagcaaaagg aatatcagct tttccatcga ttttaaattg attaaataaa
     5221 acaaatcgtt cacgttgcaa accgtcagca cttcttctac caaaacgtaa atccattaat
     5281 ttgtcatacg ttgtttctaa atcatctatg aatcgaacat ttgcagttgc tttataattg
     5341 cttaaatctt ttaattgttc gaatgtaaat tgtacttttt tatcgcctac atcacgcatt
     5401 ctagatatta tagagaagaa taaattcatt tctactgacg taaatttgcg taaaggaatc
     5461 gtatttaatt catggtggta tttcacaatt tcatttgcca taaaatcctc cttcaagatt
     5521 aactacttaa caaaattata aagagctttc taatgggggt caacccccta attaaagacc
     5581 cctaaaaaca gacaaaaaga cccctaaaaa cagacaaaaa gacccctaaa aacagacaaa
     5641 aagaccccta aaaacagaca aaaagacccc taaaagattc tgcacacctt gggagagtaa
     5701 ggatacagcc gacggtaaag aggtttaaaa agaggttata aaagagatta taaaagaggg
     5761 gggcaaaatt tcccgcctaa a
//
